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authorroot2012-05-08 18:39:56 -0500
committerroot2012-05-08 18:39:56 -0500
commitea46f42ee640928b92947bfb204c41a482d80937 (patch)
tree9b27a4eb852d12539b543c3efee9d2a47ef470f3 /web/tutorial/ppt/html/webqtl_demo2.ppt_files
parent056b5253fc3857b0444382aa39944f6344dc1ceb (diff)
downloadgenenetwork2-ea46f42ee640928b92947bfb204c41a482d80937.tar.gz
Add all the source codes into the github.
Diffstat (limited to 'web/tutorial/ppt/html/webqtl_demo2.ppt_files')
-rwxr-xr-xweb/tutorial/ppt/html/webqtl_demo2.ppt_files/Slide0001.gifbin0 -> 75611 bytes
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-rwxr-xr-xweb/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0001.htm25
-rwxr-xr-xweb/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0001_image001.pngbin0 -> 105653 bytes
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-rwxr-xr-xweb/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0002.htm25
-rwxr-xr-xweb/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0002_image002.pngbin0 -> 220529 bytes
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new file mode 100755
index 00000000..5aa0f55d
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/frame.htm
@@ -0,0 +1,43 @@
+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script><script language=JavaScript><!--
+base = parent;//-->
+</script><script language=JavaScript src=script.js></script> </head> <script> <!--
+function getSlideName() {
+ return parent.GetHrefObj( parent.g_currentSlide ).m_slideHref;
+}
+
+function getNotesName() {
+ return parent.GetHrefObj( parent.g_currentSlide ).m_notesHref;
+}
+
+function restore() {
+ self.location.reload();
+}
+
+function WriteMe() {
+ if ( true )
+ document.write( '<frameset cols=\"20%,*\" id=PPTHorizAdjust >' +
+ '<frame src=\"outline_collapsed.htm\" name=PPTOtl>' );
+ var distribution;
+ if ( parent.includeNotes )
+ distribution = "*,20%";
+ else
+ distribution = "*,0";
+ document.write('<frameset rows=\"' + distribution + '\" id=PPTVertAdjust >' +
+ '<frame src=' + getSlideName() + ' name=PPTSld>' +
+ '<frame src=' + getNotesName() + ' name=PPTNts>' );
+ document.write('</frameset>');
+ if ( true )
+ document.write(' </frameset>' );
+}
+
+if ( parent.msie < 0 ) {
+ self.onresize = restore;
+}
+
+WriteMe();
+//-->
+</script> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fs_navigation_bar.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fs_navigation_bar.htm
new file mode 100755
index 00000000..8f728017
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fs_navigation_bar.htm
@@ -0,0 +1,84 @@
+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <title>Navigation Bar</title>
+<script>
+<!--
+function restore() {
+if( navigator.platform != "MacPPC" )
+ self.location.reload();
+}
+ mainWin = parent.base;
+ dad = parent;
+function WriteBody()
+{
+ if( window.name != "PPTNav" ) return;
+
+ document.writeln( '<table border=0 valign=top cellpadding=0 cellspacing=0 height=100% width=100%> <tr> <td align=center valign=top> <table border=0 valign=top cellpadding=0 cellspacing=0 height=100%><tr><td align=right valign=top>' );
+ if ( dad.g_hideNav != 1 ) {
+ innerText = "";
+ if ( mainWin.GetHrefObj( mainWin.g_currentSlide ).m_origVisibility == 1 && !mainWin.g_allHidden)
+ innerText = "Slide " + mainWin.GetCurrentSlideNum() + " of " + mainWin.GetNumSlides();
+ else
+ innerText = "Hidden Slide";
+ if( dad.gIsEndShow )
+ innerText = "End of Slide Show";
+
+fEnable = dad.M_HasPrevSld();
+ if( false || fEnable )
+ {
+ prevBtn = "prev_active.gif";
+ document.write( '<a onclick="if(event) event.cancelBubble=true" href="javascript:dad.M_GoPrevSld()" >' );
+ }
+ else
+ prevBtn = "prev_disabled.gif";
+
+ document.write( '<img src="' + prevBtn + '" border=0 alt="Previous Slide" title="Previous Slide">' );
+
+var textAlign;
+ if ( false )
+ textAlign = 'top';
+ else
+ textAlign = 'center';
+
+ if( false || fEnable )
+ document.write( '</a>' );
+
+ document.write( '</td><td align=center valign=' + textAlign + '> &nbsp &nbsp <font face=\"Arial\" color=#000000 size=2>' + innerText + '</font> &nbsp &nbsp </td><td align=left valign=top>' );
+
+
+fEnable = (!dad.gIsEndShow && mainWin.EndSlideShow) || (!mainWin.EndSlideShow &&( mainWin.GetCurrentSlideNum() != mainWin.GetNumSlides() ) );
+ if( false || fEnable )
+ {
+ nextBtn = "next_active.gif";
+ document.write( '<a onclick="if(event) event.cancelBubble=true" href="javascript:dad.M_GoNextSld()" >' );
+ }
+ else
+ nextBtn = "next_disabled.gif";
+ document.write( '<img src="' + nextBtn + '" border=0 alt="Next Slide" title="Next Slide">' );
+
+ if( false || fEnable )
+ document.write( '</a>' );
+ document.write( '</td> </tr></table> </td> <td align=right valign=top>' );
+ document.write( '<a onclick="if(event) event.cancelBubble=true" href="javascript:mainWin.CloseFullScreen()" > ' );
+ document.write( '<img src="fullscrClose.gif"border=0 alt="Close" title="Close">');
+ document.write( ' </a>' );
+ }
+ document.write( '</td> </tr></table>' );
+}
+if ( mainWin.msie < 0 ) {
+ document.captureEvents(Event.MOUSEDOWN);
+ document.onmousedown = handleClick;
+}
+
+function handleClick(event) {
+ if ( event.which == 3 )
+ return false;
+ return true;
+}
+//-->
+</script>
+ </head> <body background=navbg.gif topmargin=2 onresize="restore()" scroll="no" onkeypress="parent.processNavKPH(event)" onclick="parent.processNavClick()"oncontextmenu="parent.processNavClick(); return false"> <center><script language=JavaScript><!--
+WriteBody();//-->
+</script></center> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscr.gif b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscr.gif
new file mode 100755
index 00000000..85842700
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscr.gif
Binary files differ
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscrClose.gif b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscrClose.gif
new file mode 100755
index 00000000..ccd0de13
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscrClose.gif
Binary files differ
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscreen.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscreen.htm
new file mode 100755
index 00000000..8cfab86e
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/fullscreen.htm
@@ -0,0 +1,48 @@
+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! opener.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script><script language=JavaScript src=script.js></script><script language=JavaScript><!--
+var SCREEN_MODE = "FullScreen";
+ base = self;
+ g_fullscrMode = 1;LoadHTMLVersion();
+//-->
+</script> </head> <script> <!--
+closing = false;
+PPTPRESENTATION = 1;
+function getSlideName() {
+ return opener.GetHrefObj( g_currentSlide ).m_slideHref;
+}
+function initpopup() {
+ if ( base.msie < 0 )
+ NavPopup = window.open( "fs_navigation_bar.htm", "controls", "alwaysRaised=yes,WIDTH=300,HEIGHT=50");
+ else
+ NavPopup = window.open( "fs_navigation_bar.htm", "controls", "WIDTH=300,HEIGHT=50");
+ NavPopup.focus();
+}
+
+
+function WriteMe() {
+ document.write( '<frameset rows=\" *,35 \" frameborder=0> ' +
+ ' ' +
+ '<frame src=\"' + getSlideName() + '\" name=PPTSld marginheight=0 marginwidth=0 scrolling=no noresize> ' +
+ ' <frame src="fs_navigation_bar.htm" name=PPTNav marginheight=0 marginwidth=0 scrolling=no noresize> ' +
+ '</frameset>' );
+ window.focus();
+}
+
+function shutdown( ) {
+if ( ! closing )
+
+ if ( window.NavPopup && !window.NavPopup.closed )
+ window.NavPopup.close();
+}
+
+WriteMe();
+// initpopup();
+
+
+ self.onunload = shutdown;
+
+//-->
+</script> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master02.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master02.htm
new file mode 100755
index 00000000..b696e328
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master02.htm
@@ -0,0 +1,11 @@
+<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Robots content=NoIndex> <link rel=Stylesheet href="master03_stylesheet.css"> <script language=JavaScript src=script.js></script>
+<script>
+<!--
+ parent.location.href=document.all.item("Main-File").href
+//-->
+</script>
+ </head> <body> <div class=O style='text-align:right;position:absolute;top:.53%;left:58.01%; width:40.79%;height:2.12%'><span style='color:black'><b>Memphis Microarray 2003</b></span></div> <div class=O style='position:absolute;top:97.52%;left:1.32%;width:42.64%; height:2.12%'><span style='color:black'><b>June 11, 2003, Rob Williams</b></span></div> <div class=O style='text-align:right;position:absolute;top:97.52%;left:58.01%; width:40.79%;height:2.12%'><span style='color:black;mso-field-code:meta16'><b>Ü#Ý</b></span><span style='color:black;mso-special-format:lastCR'><b><br> </b></span></div> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03.htm
new file mode 100755
index 00000000..a31d27f9
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03.htm
@@ -0,0 +1,11 @@
+<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Robots content=NoIndex> <link rel=Stylesheet href="master03_stylesheet.css"> <script language=JavaScript src=script.js></script>
+<script>
+<!--
+ parent.location.href=document.all.item("Main-File").href
+//-->
+</script>
+ </head> <body> <div style='position:absolute;top:.35%;left:6.09%;width:90.33%;height:18.19%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'></div> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03_background.png b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03_background.png
new file mode 100755
index 00000000..abdc767d
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03_background.png
Binary files differ
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03_stylesheet.css b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03_stylesheet.css
new file mode 100755
index 00000000..6c3dc4e2
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master03_stylesheet.css
@@ -0,0 +1 @@
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master05.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master05.htm
new file mode 100755
index 00000000..1dc23a6e
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/master05.htm
@@ -0,0 +1,11 @@
+<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Robots content=NoIndex> <link rel=Stylesheet href="master05_stylesheet.css"> <script language=JavaScript src=script.js></script>
+<script>
+<!--
+ parent.location.href=document.all.item("Main-File").href
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
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+</script> <meta name=Robots content=NoIndex> <style> <!--a {text-decoration: none;}--> </style> </head> <body bgcolor=black text=white link=white vlink=white alink=white onload=" parent.base.highlite();" onresize="self.location.reload();"> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID1 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p1" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('1')" id=AREF>The GeneNetwork and WebQTL : PART 2<span style="mso-spacerun: yes">&nbsp;&nbsp; </span><br> link to www.genenetwork.org</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID2 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p2" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('2')" id=AREF>Slide 2</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID3 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p3" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('3')" id=AREF>Slide 3</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID4 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p4" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('4')" id=AREF><font face=Verdana>Discovering upstream modulatory loci</font></a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID5 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p5" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('5')" id=AREF>WebQTL searches for upstream controllers</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID6 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p6" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('6')" id=AREF>Genetic versus Physical maps for App expression</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID7 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p7" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('7')" id=AREF>Physical map for distal chromosome 7</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID8 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p8" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('8')" id=AREF>Evaluating candidate genes</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID9 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p9" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('9')" id=AREF>Physical maps are zoomable</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID10 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p10" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('10')" id=AREF>Evaluating Ctbp2 as a candidate QTL for App</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID11 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p11" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('11')" id=AREF>Evaluating Ctbp2 using other resources</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID12 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p12" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('12')" id=AREF>&quot;Summary of Part 2&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID13 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p13" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('13')" id=AREF>Test Questions</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID14 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p14" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('14')" id=AREF>Contact for comments and improvements:</a></b></font></td> </tr> </table> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_expand.gif b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_expand.gif
new file mode 100755
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_expanded.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_expanded.htm
new file mode 100755
index 00000000..c33cf334
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_expanded.htm
@@ -0,0 +1,5 @@
+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Robots content=NoIndex> <style> <!--a {text-decoration: none;}--> </style> </head> <body bgcolor=black text=white link=white vlink=white alink=white onload=" parent.base.highlite();" onresize="self.location.reload();"> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID1 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p1" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('1')" id=AREF>The GeneNetwork and WebQTL : PART 2<span style="mso-spacerun: yes">&nbsp;&nbsp; </span><br> link to www.genenetwork.org</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Part 1. How to study expression variation and genetic correlation (slides 2&#8211;17)</font></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Part 2. Discovering upstream modulators (slides 18&#8211;29)</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID2 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p2" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('2')" id=AREF>Slide 2</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID3 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p3" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('3')" id=AREF>Slide 3</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID4 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p4" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('4')" id=AREF><font face=Verdana>Discovering upstream modulatory loci</font></a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID5 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p5" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('5')" id=AREF>WebQTL searches for upstream controllers</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID6 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p6" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('6')" id=AREF>Genetic versus Physical maps for App expression</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID7 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p7" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('7')" id=AREF>Physical map for distal chromosome 7</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID8 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p8" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('8')" id=AREF>Evaluating candidate genes</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID9 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p9" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('9')" id=AREF>Physical maps are zoomable</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID10 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p10" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('10')" id=AREF>Evaluating Ctbp2 as a candidate QTL for App</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID11 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p11" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('11')" id=AREF>Evaluating Ctbp2 using other resources</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID12 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p12" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('12')" id=AREF>&quot;Summary of Part 2&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Summary of Part 2</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID13 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p13" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('13')" id=AREF>Test Questions</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID14 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p14" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('14')" id=AREF>Contact for comments and improvements:</a></b></font></td> </tr> </table> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_navigation_bar.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_navigation_bar.htm
new file mode 100755
index 00000000..85b11c52
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/outline_navigation_bar.htm
@@ -0,0 +1,37 @@
+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script><script language=JavaScript><!--
+
+function restore() {
+if( navigator.platform != "MacPPC" )
+ self.location.reload();
+}
+
+function writebody() {
+
+ if ( parent.g_showoutline ) {
+ document.write('<table border=0 valign=top cellspacing=0 width=\"100%\">' +
+ '<tr valign=top><td align=left valign=top>' );
+ document.write( '<a href=\"javascript:parent.ToggleOutline()\" ><img src=\"outline.gif\" title=\"Show/Hide Outline\" alt=\"Show/Hide Outline\" border=0></a>' );
+ document.write( '</td>' );
+ if( parent.g_outline_href == parent.OUTLINE_EXPAND_HREF ) {
+ document.write( '<td align=right valign=top><a href=\"javascript:parent.CollapseOutline()\">' +
+ '<img src="outline_collapse.gif" title="Collapse Outline" alt="Collapse Outline" border=0>' +
+ '</a></td>');
+ } else {
+ document.write('<td align=right valign=top><a href=\"javascript:parent.ExpandOutline()\">' +
+ '<img src="outline_expand.gif" title="Expand Outline" alt="Expand Outline" border=0>' +
+ '</a></td>');
+ }
+ document.write("</tr></table>");
+ }
+ else {
+ document.write( '<table border=0 valign=top cellspacing=0 width=\"100%\"><td align=\"left\"><a href=\"javascript:parent.ToggleOutline()\" ><img border=0 src=\"outline.gif\" title=\"Show/Hide Outline\" alt=\"Show/Hide Outline\"></a></td></table>' );
+ }
+}//-->
+</script> </head> <body background=navbg.gif topmargin=1 onresize="restore()" scroll="no" > <script language=JavaScript><!--
+writebody();
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/prev_active.gif b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/prev_active.gif
new file mode 100755
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/prev_disabled.gif b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/prev_disabled.gif
new file mode 100755
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Binary files differ
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/script.js b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/script.js
new file mode 100755
index 00000000..8110ab9a
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/script.js
@@ -0,0 +1,1343 @@
+var CtxAlwaysOn = false;
+function LoadSld( slideId )
+{
+ if( !g_supportsPPTHTML ) return
+ if( slideId )
+ parent.base.SldUpdated(slideId)
+ g_origSz=parseInt(SlideObj.style.fontSize)
+ g_origH=SlideObj.style.posHeight
+ g_origW=SlideObj.style.posWidth
+ g_scaleHyperlinks=(document.all.tags("AREA").length>0)
+ if ( IsWin("PPTSld") && !parent.IsFullScrMode() )
+ parent.base.highlite();
+ if( g_scaleHyperlinks )
+ InitHLinkArray()
+ if( g_scaleInFrame||(IsWin("PPTSld") && parent.IsFullScrMode() ) )
+ document.body.scroll="no"
+ _RSW()
+ if( IsWin("PPTSld") && (parent.IsFullScrMode() || CtxAlwaysOn ) ) {
+ document.oncontextmenu=parent._CM;
+ self.focus();
+
+ }
+}
+function MakeSldVis( fTrans )
+{
+ fTrans=fTrans && g_showAnimation
+ if( fTrans )
+ {
+ if( g_bgSound ) {
+ idx=g_bgSound.indexOf(",");
+ pptSound.src=g_bgSound.substr( 0, idx );
+ pptSound.loop= -(parseInt(g_bgSound.substr(idx+1)));
+ }
+ SlideObj.filters.revealtrans.Apply()
+ }
+ SlideObj.style.visibility="visible"
+ if( fTrans )
+ SlideObj.filters.revealtrans.Play()
+}
+function MakeNotesVis()
+{
+ if( !IsNts() ) return false
+ SlideObj.style.display="none"
+ nObj = document.all.item("NotesObj")
+ parent.SetHasNts(0)
+ if( nObj ) {
+ nObj.style.display=""
+ parent.SetHasNts(1)
+ }
+ return 1
+}
+function Redirect( frmId,sId )
+{
+ var str=document.location.hash,idx=str.indexOf('#')
+ if(idx>=0) str=str.substr(1);
+ if( window.name != frmId && ( sId != str) ) {
+ obj = document.all.item("Main-File")
+ window.location.href=obj.href+"#"+sId
+ return 1
+ }
+ return 0
+}
+function HideMenu() { if( frames["PPTSld"] && PPTSld.document.all.item("ctxtmenu") && PPTSld.ctxtmenu.style.display!="none" ) { PPTSld.ctxtmenu.style.display='none'; return true } return false }
+function IsWin( name ) { return window.name == name }
+function IsNts() { return IsWin("PPTNts") }
+function IsSldOrNts() { return( IsWin("PPTSld")||IsWin("PPTNts") ) }
+function SupportsPPTAnimation() { return( navigator.platform == "Win32" && navigator.appVersion.indexOf("Windows")>0 ) }
+function SupportsPPTHTML()
+{
+ var appVer=navigator.appVersion, msie=appVer.indexOf( "MSIE " ), inex = appVer.indexOf( "Internet Explorer " ), ver=0
+ if( msie >= 0 )
+ ver=parseFloat( appVer.substring( msie+5, appVer.indexOf(";",msie) ) )
+ else if( inex >= 0 )
+ ver=parseFloat( appVer.substring( inex+18, appVer.indexOf(";",inex) ) )
+ else
+ ver=parseInt(appVer)
+
+ return( ver >= 4 )
+}
+var MHTMLPrefix = CalculateMHTMLPrefix();
+function CalculateMHTMLPrefix()
+{
+ if ( document.location.protocol == 'mhtml:') {
+ href=new String(document.location.href)
+ Start=href.indexOf('!')+1
+ End=href.lastIndexOf('/')+1
+ if (End < Start)
+ return href.substring(0, Start)
+ else
+ return href.substring(0, End)
+ }
+ return '';
+}
+
+function LoadNavSld(slideId) {
+playList();
+parent.createCM();
+ if( !g_supportsPPTHTML ) return
+ if( IsWin("PPTSld") && slideId )
+ parent.base.SldUpdated(slideId)
+ self.focus();
+
+}
+var hasNarration = false;
+function _RSW()
+{
+ if( !g_supportsPPTHTML || IsNts() ||
+ ( !g_scaleInFrame && (( window.name != "PPTSld" ) ) ) )
+ return
+
+ cltWidth=document.body.clientWidth
+ cltHeight=document.body.clientHeight
+ factor=(1.0*cltWidth)/g_origW
+ if( cltHeight < g_origH*factor )
+ factor=(1.0*cltHeight)/g_origH
+
+ newSize = g_origSz * factor
+ if( newSize < 1 ) newSize=1
+
+ s=SlideObj.style
+ s.fontSize=newSize+"px"
+ s.posWidth=g_origW*factor
+ s.posHeight=g_origH*factor
+ s.posLeft=(cltWidth-s.posWidth)/2
+ s.posTop=(cltHeight-s.posHeight)/2
+
+ if ( hasNarration ) {
+ obj = document.all.NSPlay.style;
+ mySld = document.all.SlideObj.style;
+ obj.position = 'absolute';
+ obj.posTop = mySld.posTop + mySld.posHeight - 20;
+ obj.posLeft = mySld.posLeft + mySld.posWidth - 20;
+ }
+ if( g_scaleHyperlinks )
+ ScaleHyperlinks( factor );
+}
+function IsMac() {
+ return (window.navigator.platform.indexOf("Mac") >= 0 );
+}
+
+function HitOK( evt ) {
+ //Nav Only function
+ return (evt.which == 1 || (IsMac() && evt.which == 3) );
+}
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+ if ( parent.base.msie < 0 ) {
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+ return parent.KPH(event);
+ else if( (event.which == 32) || (event.which == 13) || HitOK( event ) ) {
+ if( window.name == "PPTSld" )
+ parent.PPTSld.DocumentOnClick();
+ else
+ parent.M_GoNextSld();
+ }
+ else if ( parent.IsFullScrMode() && ((event.which == 78) || (event.which == 110) || (event.which == 29) || (event.which == 31) || (event.which == 12)) )
+ parent.M_GoNextSld();
+ else if ( parent.IsFullScrMode() && ( (event.which == 80) || (event.which == 112) || (event.which == 30) || (event.which == 28) || (event.which == 11) || (event.which == 8)) )
+ parent.M_GoPrevSld();
+
+ return;
+ }
+
+ if( IsNts() ) return;
+
+ if(parent.IsFullScrMode() && event.keyCode == 27 && !parent.HideMenu() )
+ parent.base.CloseFullScreen();
+ else if( (event.keyCode == 32) || (event.keyCode == 13) )
+ {
+ if( window.name == "PPTSld" )
+ parent.PPTSld.DocumentOnClick();
+ else
+ parent.M_GoNextSld();
+ }
+ else if ( parent.IsFullScrMode() && ((event.keyCode == 78) || (event.keyCode == 110)) )
+ parent.M_GoNextSld();
+ else if ( parent.IsFullScrMode() && ((event.keyCode == 80) || (event.keyCode == 112)) )
+ parent.M_GoPrevSld();
+}
+
+function DocumentOnClick(event)
+{
+ if ( g_doAdvOnClick && !parent.IsFullScrMode() ) {
+ parent.base.TP_GoToNextSld();
+ return;
+ }
+
+ if ( parent.base.msie < 0 )
+ {
+ if( ( g_allowAdvOnClick && parent.IsFullScrMode() ) || g_doAdvOnClick ||
+ (event && ( (event.which == 32) || (event.which == 13) ) ) )
+ parent.M_GoNextSld();
+ return;
+ }
+ if( IsNts() || (parent.IsFullScrMode() && parent.HideMenu() ) ) return;
+ if( ( g_allowAdvOnClick && parent.IsFullScrMode() ) || g_doAdvOnClick ||
+ (event && ( (event.keyCode==32) || (event.keyCode == 13) ) ) )
+ parent.M_GoNextSld();
+}
+
+
+var g_supportsPPTHTML = SupportsPPTHTML(), g_scaleInFrame = true, gId="", g_bgSound="",
+ g_scaleHyperlinks = false, g_allowAdvOnClick = true, g_showInBrowser = false, g_doAdvOnClick = false;
+
+ var g_showAnimation = 0;
+var g_hasTrans = false, g_autoTrans = false, g_transSecs = 0;
+var g_animManager = null;
+
+var ENDSHOW_MESG="End of slide show, click to exit.", SCREEN_MODE="Frames", gIsEndShow=0, NUM_VIS_SLDS=14, SCRIPT_HREF="script.js", FULLSCR_HREF="fullscreen.htm";
+var gCurSld = gPrevSld = 1, g_offset = 0, gNtsOpen = gHasNts = gOtlTxtExp = gNarrationPaused = false, gOtlOpen = true
+window.gPPTHTML=SupportsPPTHTML()
+var g_hideNav = 0;
+function UpdNtsPane(){ PPTNts.location.replace( MHTMLPrefix+GetHrefObj( gCurSld ).mNtsHref ) }
+function UpdNavPane( sldIndex ){ if(gNavLoaded) PPTNav.UpdNav() }
+function UpdOtNavPane(){ if(gOtlNavLoaded) PPTOtlNav.UpdOtlNav() }
+function UpdOtlPane(){ if(gOtlLoaded) PPTOtl.UpdOtl() }
+function SetHasNts( fVal )
+{
+ if( gHasNts != fVal ) {
+ gHasNts=fVal
+ UpdNavPane()
+ }
+}
+
+function ToggleVNarration()
+{
+ if ( base.msie < 0 ) {
+ PPTSld.ToggleSound( false, PPTSld.document.NSPlay );
+ return;
+ }
+
+ rObj=PPTSld.document.all("NSPlay")
+ if( rObj ) {
+ if( gNarrationPaused )
+ rObj.Play()
+ else
+ rObj.Pause()
+
+ gNarrationPaused=!gNarrationPaused
+ }
+}
+
+function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) }
+function HasPrevSld() { return ( gIsEndShow || ( g_currentSlide != 1 && GetHrefObj( g_currentSlide-1 ).mVis == 1 )||( GetCurrentSlideNum() > 1 ) ) }
+function HasNextSld() { return (GetCurrentSlideNum() != GetNumSlides()) }
+function StartEndShow()
+{
+// g_hideNav = 1;
+// PPTNav.location.reload();
+ if( PPTSld.event ) PPTSld.event.cancelBubble=true
+
+ doc=PPTSld.document
+ doc.open()
+ doc.writeln('<html><head><script > /*defer>*/ g_ctxmenu = 0; ' +
+ 'if( parent.base.msie < 0 ) { document.captureEvents(Event.KEYPRESS); document.captureEvents(Event.MOUSEDOWN); document.onkeypress = _KPH; document.onmousedown = _KPH; } ' +
+ 'function DocumentOnClick(event) { return _KPH(event); } function IsContextMenu() { return (g_ctxmenu ==1); } ' +
+ 'function _KPH(event)' +
+ '{ ' +
+ 'if ( parent.base.msie < 0 && (parent.IsFullScrMode() ) && event ) { if ( (!parent.IsMac() && event.which == 3) || ( parent.IsMac() && (event.modifiers & Event.CONTROL_MASK) && event.which == 1 ) ) { return parent.KPH(event); } ' +
+ ' else if (event.which == 27 || event.which == 32 || event.which == 13 || parent.HitOK( event ) || (event.which == 78) || (event.which == 110) || (event.which == 29) || (event.which == 31) || (event.which == 12) ) { if ( IsContextMenu() ) { return parent.KPH(event); } parent.base.CloseFullScreen(); return; } ' +
+ ' else if ( (event.which == 80) || (event.which == 112) || (event.which == 30) || (event.which == 28) || (event.which == 11) || (event.which == 8) ) { parent.M_GoPrevSld(); } } ' +
+ 'if( parent.HideMenu() ) return; if( (parent.IsFullScrMode() ) && event) { if ( (event.keyCode==27 || event.keyCode == 13 || event.keyCode==32 || event.type=="click" ) || (event.keyCode == 78) || (event.keyCode == 110) ) { parent.base.CloseFullScreen(); }' +
+ ' else if ( (event.keyCode == 80) || (event.keyCode == 112) ) { parent.M_GoPrevSld(); } } } function Unload() { parent.gIsEndShow=0; } function SetupEndShow() { if ( !parent.IsFullScrMode() ) { return; } else { parent.PPTNav.location.reload(); } parent.gIsEndShow=1; if ( parent.g_docTable[0].type != "jpeg" ) { if ( parent.base.msie < 0 ) {parent.createCM(); } document.oncontextmenu=parent._CM; } }</script></head><body scroll=\"no\" onclick=\"DocumentOnClick(event)\" onkeypress=\"_KPH(event)\" bgcolor=\"black\" onload=\"SetupEndShow()\" onunload=\"Unload()\"><center><p><font face=Tahoma color=white size=2><br><b>' + ENDSHOW_MESG + '</b></font></p></center></body></html>')
+ doc.close()
+}
+function SetSldVisited(){ gDocTable[gCurSld-1].mVisited=true }
+function IsSldVisited(){ return gDocTable[gCurSld-1].mVisited }
+function hrefList( sldHref, visible, sldIdx )
+{
+ this.mSldHref= this.mNtsHref = sldHref
+ this.mSldIdx = sldIdx
+ this.mOrigVis= this.mVis = visible
+ this.mVisited= false
+}
+var gDocTable = new Array(
+ new hrefList("slide0001.htm", 1, 1),
+ new hrefList("slide0002.htm", 1, 2),
+ new hrefList("slide0003.htm", 1, 3),
+ new hrefList("slide0004.htm", 1, 4),
+ new hrefList("slide0005.htm", 1, 5),
+ new hrefList("slide0006.htm", 1, 6),
+ new hrefList("slide0007.htm", 1, 7),
+ new hrefList("slide0008.htm", 1, 8),
+ new hrefList("slide0009.htm", 1, 9),
+ new hrefList("slide0010.htm", 1, 10),
+ new hrefList("slide0011.htm", 1, 11),
+ new hrefList("slide0012.htm", 1, 12),
+ new hrefList("slide0013.htm", 1, 13),
+ new hrefList("slide0014.htm", 1, 14)
+);
+
+function ImgBtn( oId,bId,w,action )
+{
+ var t=this
+ t.Perform = _IBP
+ t.SetActive = _IBSetA
+ t.SetInactive= _IBSetI
+ t.SetPressed = _IBSetP
+ t.SetDisabled= _IBSetD
+ t.Enabled = _IBSetE
+ t.ChangeIcon = null
+ t.UserAction = action
+ t.ChgState = _IBUI
+ t.mObjId = oId
+ t.mBorderId= bId
+ t.mWidth = w
+ t.mIsOn = t.mCurState = 0
+}
+function _IBSetA()
+{
+ if( this.mIsOn ) {
+ obj=this.ChgState( gHiliteClr,gShadowClr,2 )
+ obj.style.posTop=0
+ }
+}
+function _IBSetI()
+{
+ if( this.mIsOn ) {
+ obj=this.ChgState( gFaceClr,gFaceClr,1 )
+ obj.style.posTop=0
+ }
+}
+function _IBSetP()
+{
+ if( this.mIsOn ) {
+ obj=this.ChgState( gShadowClr,gHiliteClr,2 )
+ obj.style.posLeft+=1; obj.style.posTop+=1
+ }
+}
+function _IBSetD()
+{
+ obj=this.ChgState( gFaceClr,gFaceClr,0 )
+ obj.style.posTop=0
+}
+function _IBSetE( state )
+{
+ var t=this
+ GetObj( t.mBorderId ).style.visibility="visible"
+ if( state != t.mIsOn ) {
+ t.mIsOn=state
+ if( state )
+ t.SetInactive()
+ else
+ t.SetDisabled()
+ }
+}
+function _IBP()
+{
+ var t=this
+ if( t.mIsOn ) {
+ if( t.UserAction != null )
+ t.UserAction()
+ if( t.ChangeIcon ) {
+ obj=GetObj(t.mObjId)
+ if( t.ChangeIcon() )
+ obj.style.posLeft=obj.style.posLeft+(t.mCurState-4)*t.mWidth
+ else
+ obj.style.posLeft=obj.style.posLeft+(t.mCurState-0)*t.mWidth
+ }
+ t.SetActive()
+ }
+}
+function _IBUI( clr1,clr2,nextState )
+{
+ var t=this
+ SetBorder( GetObj( t.mBorderId ),clr1,clr2 )
+ obj=GetObj( t.mObjId )
+ obj.style.posLeft=obj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj.style.posTop
+ t.mCurState=nextState
+ return obj
+}
+function TxtBtn( oId,oeId,action,chkState )
+{
+ var t=this
+ t.Perform = _TBP
+ t.SetActive = _TBSetA
+ t.SetInactive= _TBSetI
+ t.SetPressed = _TBSetP
+ t.SetDisabled= _TBSetD
+ t.SetEnabled = _TBSetE
+ t.GetState = chkState
+ t.UserAction = action
+ t.ChgState = _TBUI
+ t.mObjId = oId
+ t.m_elementsId= oeId
+ t.mIsOn = 1
+}
+function _TBSetA()
+{
+ var t=this
+ if( t.mIsOn && !t.GetState() )
+ t.ChgState( gHiliteClr,gShadowClr,0,0 )
+}
+function _TBSetI()
+{
+ var t=this
+ if( t.mIsOn && !t.GetState() )
+ t.ChgState( gFaceClr,gFaceClr,0,0 )
+}
+function _TBSetP()
+{
+ if( this.mIsOn )
+ this.ChgState( gShadowClr,gHiliteClr,1,1 )
+}
+function _TBSetD()
+{
+ this.ChgState( gFaceClr,gFaceClr,0,0 )
+ this.mIsOn = 0
+}
+function _TBSetE()
+{
+ var t=this
+ if( !t.GetState() )
+ t.ChgState( gFaceClr,gFaceClr,0,0 )
+ else
+ t.ChgState( gShadowClr,gHiliteClr,1,1 )
+ t.mIsOn = 1
+}
+function _TBP()
+{
+ var t=this
+ if( t.mIsOn ) {
+ if( t.UserAction != null )
+ t.UserAction()
+ if( t.GetState() )
+ t.SetPressed()
+ else
+ t.SetActive()
+ }
+}
+function _TBUI( clr1,clr2,lOffset,tOffset )
+{
+ SetBorder( GetObj( this.mObjId ),clr1,clr2 )
+ Offset( GetObj( this.m_elementsId ),lOffset,tOffset )
+}
+function GetObj( objId ){ return document.all.item( objId ) }
+function Offset( obj, top, left ){ obj.style.top=top; obj.style.left=left }
+function SetBorder( obj, upperLeft, lowerRight )
+{
+ s=obj.style;
+ s.borderStyle = "solid"
+ s.borderWidth = 1
+ s.borderLeftColor = s.borderTopColor = upperLeft
+ s.borderBottomColor= s.borderRightColor = lowerRight
+}
+function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] }
+function BtnOnOver(){ b=GetBtnObj(); if( b != null ) b.SetActive() }
+function BtnOnDown(){ b=GetBtnObj(); if( b != null ) b.SetPressed() }
+function BtnOnOut(){ b=GetBtnObj(); if( b != null ) b.SetInactive() }
+function BtnOnUp()
+{
+ b=GetBtnObj()
+ if( b != null )
+ b.Perform()
+ else
+ Upd()
+}
+function GetNtsState(){ return parent.gNtsOpen }
+function GetOtlState(){ return parent.gOtlOpen }
+function GetOtlTxtState(){ return parent.gOtlTxtExp }
+function NtsBtnSetFlag( fVal )
+{
+ s=document.all.item( this.m_flagId ).style
+ s.display="none"
+ if( fVal )
+ s.display=""
+ else
+ s.display="none"
+}
+
+var gHiliteClr="THREEDHIGHLIGHT",gShadowClr="THREEDSHADOW",gFaceClr="THREEDFACE"
+var gBtnArr = new Array()
+gBtnArr["nb_otl"] = new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPane,GetOtlState )
+gBtnArr["nb_nts"] = new TxtBtn( "nb_nts","nb_ntsElem",parent.ToggleNtsPane,GetNtsState )
+gBtnArr["nb_prev"]= new ImgBtn( "nb_prev","nb_prevBorder",30,parent.GoToPrevSld )
+gBtnArr["nb_next"]= new ImgBtn( "nb_next","nb_nextBorder",30,parent.GoToNextSld )
+gBtnArr["nb_sldshw"]= new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent.FullScreen )
+gBtnArr["nb_voice"] = new ImgBtn( "nb_voice","nb_voiceBorder",18,parent.ToggleVNarration )
+gBtnArr["nb_otlTxt"]= new ImgBtn( "nb_otlTxt","nb_otlTxtBorder",23,parent.ToggleOtlText )
+gBtnArr["nb_nts"].m_flagId= "notes_flag"
+gBtnArr["nb_nts"].SetFlag = NtsBtnSetFlag
+gBtnArr["nb_otlTxt"].ChangeIcon= GetOtlTxtState
+var sNext="Next",sPrev="Previous",sEnd="End Show",sFont="Arial", alwaysOn = false
+function ShowMenu()
+{
+ BuildMenu();
+ var doc=PPTSld.document.body,x=PPTSld.event.clientX+doc.scrollLeft,y=PPTSld.event.clientY+doc.scrollTop
+
+ m = PPTSld.document.all.item("ctxtmenu")
+ m.style.pixelLeft=x
+ if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) )
+ m.style.pixelLeft=x-m.scrollWidth
+
+ m.style.pixelTop=y
+ if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) )
+ m.style.pixelTop=y-m.scrollHeight
+
+ m.style.display=""
+}
+function _CM()
+{
+ if( !parent.IsFullScrMode() && !alwaysOn) return;
+
+ if(!PPTSld.event.ctrlKey) {
+ ShowMenu()
+ return false
+ } else
+ HideMenu()
+}
+
+function processNavKPH(event) {
+ if ( PPTSld && (event.keyCode != 13 || !event.srcElement.href || event.srcElement.href == "" ) )
+ return PPTSld._KPH(event);
+}
+function processNavClick() {
+ HideMenu();
+ return true;
+}
+function BuildMenu()
+{
+ if( PPTSld.document.all.item("ctxtmenu") ) return
+
+ var mObj=CreateItem( PPTSld.document.body )
+mObj.id="ctxtmenu"
+ var s=mObj.style
+ s.position="absolute"
+ s.cursor="default"
+ s.width="100px"
+ SetCMBorder(mObj,"menu","black")
+
+ var iObj=CreateItem( mObj )
+ SetCMBorder( iObj, "threedhighlight","threedshadow" )
+ iObj.style.padding=2
+ if ( self.IsFullScrMode() ) {
+ CreateMenuItem( iObj,sNext,M_GoNextSld,M_True )
+ CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld )
+ }
+ else {
+ CreateMenuItem( iObj,sNext, base.TP_GoToNextSld, base.HasNextSld )
+ CreateMenuItem( iObj,sPrev,base.GoToPrevSld, base.HasPrevSld )
+ }
+ var sObj=CreateItem( iObj )
+ SetCMBorder(sObj,"menu","menu")
+ var s=sObj.style
+ s.borderTopColor="threedshadow"
+ s.borderBottomColor="threedhighlight"
+ s.height=1
+ s.fontSize="0px"
+ if ( self.IsFullScrMode() )
+ CreateMenuItem( iObj,sEnd,M_End,M_True )
+ else
+ CreateMenuItem( iObj,sEnd,M_End,M_False )
+}
+function Highlight() { ChangeClr("activecaption","threedhighlight") }
+function Deselect() { ChangeClr("threedface","menutext") }
+function Perform()
+{
+ e=PPTSld.event.srcElement
+ if( e.type=="menuitem" && e.IsActive() )
+ e.Action()
+ else
+ PPTSld.event.cancelBubble=true
+}
+function ChangeClr( bg,clr )
+{
+ e=PPTSld.event.srcElement
+ if( e.type=="menuitem" && e.IsActive() ) {
+ e.style.backgroundColor=bg
+ e.style.color=clr
+ }
+}
+
+function M_HasPrevSld() { return( base.HasPrevSld() ) }
+function M_GoNextSld() {
+ base.SetFSMode(1);
+ if( gIsEndShow )
+ M_End();
+ else {
+ if ( base.HasNextSld() )
+ base.GoToNextSld();
+ else if ( base.EndSlideShow ) {
+ StartEndShow();
+ gIsEndShow = 1;
+
+ PPTNav.location.reload();
+ }
+ else
+ base.CloseFullScreen();
+ }
+}
+function M_GoPrevSld() {
+ base.SetFSMode(1);
+ g_hideNav = 0;
+ if( gIsEndShow ) {
+ gIsEndShow = 0;
+ if ( base.msie > 0 && IsMac() )
+ ChangeFrame( SLIDE_FRAME, GetHrefObj( g_currentSlide ).m_slideHref );
+ else
+ PPTSld.history.back();
+
+ PPTNav.location.reload();
+ if( PPTSld.event )
+ PPTSld.event.cancelBubble=true;
+ }
+ else
+ base.GoToPrevSld();
+}
+function M_True() { return true }
+function M_False() { return false }
+
+function M_End() {
+ base.CloseFullScreen();
+ /*PPTSld.event.cancelBubble=true;
+ window.close( self )*/
+}
+
+function CreateMenuItem( node,text,action,eval )
+{
+ var e=CreateItem( node )
+ e.type="menuitem"
+ e.Action=action
+ e.IsActive=eval
+ e.innerHTML=text
+
+ if( !e.IsActive() )
+ e.style.color="threedshadow"
+ e.onclick=Perform
+ e.onmouseover=Highlight
+ e.onmouseout=Deselect
+ s=e.style;
+ s.fontFamily=sFont
+ s.fontSize="8pt"
+ s.paddingLeft=2
+}
+function CreateItem( node )
+{
+ var elem=PPTSld.document.createElement("DIV")
+ node.insertBefore( elem )
+ return elem
+}
+function SetCMBorder( o,ltClr,rbClr )
+{
+ var s=o.style
+ s.backgroundColor="menu"
+ s.borderStyle="solid"
+ s.borderWidth=1
+ s.borderColor=ltClr+" "+rbClr+" "+rbClr+" "+ltClr
+}
+
+/* netscape context menu */
+g_ctxmenu = 0;
+function setRect( obj, X, Y, W, H ) {
+ obj.top = Y;
+ obj.left = X;
+ obj.clip.top = 0;
+ obj.clip.left = 0;
+ obj.clip.bottom = H;
+ obj.clip.right = W;
+}
+
+function KPH(event) {
+ if ( ! base.IsFullScrMode() && !alwaysOn )
+ return true;
+
+ if ( (!IsMac() &&event.which == 3) || ( IsMac() && (event.modifiers & Event.CONTROL_MASK) && event.which == 1 ) ) {
+ PPTSld.g_ctxmenu = 1;
+ PPTSld.stripUobj.visibility = "show";
+ PPTSld.stripDobj.visibility = "show";
+ PPTSld.shadeUobj.visibility = "show";
+ PPTSld.shadeDobj.visibility = "show";
+ PPTSld.panelobj.visibility = "show";
+ PPTSld.Fobj.visibility = "show";
+ PPTSld.Bobj.visibility = "show";
+ PPTSld.Eobj.visibility = "show";
+
+ setRect(PPTSld.shadeUobj, event.pageX-2, event.pageY-2, 82, 67 );
+ setRect(PPTSld.shadeDobj, event.pageX, event.pageY, 82, 67 );
+ setRect(PPTSld.panelobj, event.pageX, event.pageY, 80, 65 );
+ setRect(PPTSld.Fobj, event.pageX, event.pageY, 80, 20 );
+ setRect(PPTSld.Bobj, event.pageX, event.pageY+20, 80, 20 );
+ setRect(PPTSld.stripUobj, event.pageX, event.pageY+41, 80, 1 );
+ setRect(PPTSld.stripDobj, event.pageX, event.pageY+43, 80, 1 );
+ setRect(PPTSld.Eobj, event.pageX, event.pageY+45, 80, 20 );
+ return false;
+ }
+ if ( HitOK( event ) ) {
+ PPTSld.g_ctxmenu = 0;
+ PPTSld.stripUobj.visibility = "hide";
+ PPTSld.stripDobj.visibility = "hide";
+ PPTSld.shadeUobj.visibility = "hide";
+ PPTSld.shadeDobj.visibility = "hide";
+ PPTSld.panelobj.visibility = "hide";
+ PPTSld.Fobj.visibility = "hide";
+ PPTSld.Bobj.visibility = "hide";
+ PPTSld.Eobj.visibility = "hide";
+ }
+ return true;
+}
+
+function overMe() {
+ this.bgColor = "blue";
+}
+
+function outMe() {
+ this.bgColor = "#AAAAAA";
+}
+
+function makeElement( whichEl, whichContainer ) {
+ if ( arguments.length == 1 ) {
+ whichContainer = PPTSld;
+ }
+ tmp = new Layer(100,whichContainer);
+ eval( whichEl + " = tmp" );
+ return eval(whichEl);
+}
+
+function initMe( obj, clr, text ) {
+ obj.bgColor = clr;
+// obj.document.write("<a href='javascript:return false'>" + text + "</a>");
+ obj.document.write( "<font size=2 face=Arial " );
+ if ( !M_HasPrevSld() && (obj == PPTSld.Bobj ) ) {
+ obj.document.write( " color='#808080' " );
+ }
+ else {
+ obj.onmouseover = overMe;
+ obj.onmouseout = outMe;
+ }
+ obj.document.write( " > &nbsp " + text +"</font> <layer top=0 left=0 width=100 height=40 ></layer>");
+ obj.document.close();
+ obj.captureEvents(Event.CLICK);
+ obj.color = "black";
+}
+
+function createCM() {
+ if ( base.IsFullScrMode() ) {
+ var clr = "#AAAAAA";
+ PPTSld.shadeUobj = makeElement("SHADEU");
+ PPTSld.shadeDobj = makeElement("SHADED");
+ PPTSld.panelobj = makeElement("PANEL");
+ PPTSld.stripUobj = makeElement("STRIPU");
+ PPTSld.stripDobj = makeElement("STRIPD");
+ PPTSld.shadeUobj.bgColor = "#BBBBBB";
+ PPTSld.shadeDobj.bgColor = "#888888";
+ PPTSld.stripUobj.bgColor = "#777777";
+ PPTSld.stripDobj.bgColor = "#CCCCCC";
+ PPTSld.panelobj.bgColor = clr;
+ PPTSld.Fobj = makeElement("Next");
+ PPTSld.Bobj = makeElement("Previous");
+ PPTSld.Eobj = makeElement("EndShow");
+ initMe( PPTSld.Fobj, clr, "Next" );
+ PPTSld.Fobj.onclick = M_GoNextSld;
+
+ initMe( PPTSld.Bobj, clr, "Previous" );
+ PPTSld.Bobj.onclick = M_GoPrevSld;
+
+ initMe( PPTSld.Eobj, clr, "End Show");
+ PPTSld.Eobj.onclick = base.CloseFullScreen;
+ }
+}
+
+function IsContextMenu() {
+ return (g_ctxmenu == 1)
+}
+var g_notesTable = new Array()
+var g_hiddenSlide = new Array()
+makeSlide( 0,1,1);
+makeSlide( 1,1,1);
+makeSlide( 2,1,1);
+makeSlide( 3,1,1);
+makeSlide( 4,1,1);
+makeSlide( 5,1,1);
+makeSlide( 6,1,1);
+makeSlide( 7,1,1);
+makeSlide( 8,1,1);
+makeSlide( 9,1,1);
+makeSlide( 10,1,1);
+makeSlide( 11,0,1);
+makeSlide( 12,0,1);
+makeSlide( 13,1,1);
+
+var END_SHOW_HREF = "endshow.htm",
+ OUTLINE_EXPAND_HREF = "outline_expanded.htm",
+ OUTLINE_COLLAPSE_HREF = "outline_collapsed.htm",
+ OUTLINE_NAVBAR_HREF = "outline_navigation_bar.htm",
+ NAVBAR_HREF = "navigation_bar.htm",
+ BLANK_NOTES_HREF = "blank_notes.htm",
+ NUM_VISIBLE_SLIDES = 14,
+ SIMPLE_FRAMESET = 0,
+ SLIDE_FRAME = "PPTSld",
+ NOTES_FRAME = "PPTNts",
+ OUTLINE_FRAME = "PPTOtl",
+ OUTLINE_NAVBAR_FRAME = "PPTOtlNav",
+ NAVBAR_FRAME = "PPTNav",
+ MAIN_FRAME = "MainFrame",
+ FS_NAVBAR_HREF = "fs_navigation_bar.htm",
+ isIEFiles = 2,
+ isNAVFiles = 8,
+ isFLATFiles = 16,
+ includeNotes = 1,
+ PPTPRESENTATION = 1;
+var INITSLIDENUM = 1;
+
+var EndSlideShow = 0;
+var g_outline_href = OUTLINE_COLLAPSE_HREF;
+var g_fullscrMode = 0;
+var FSWin = null;
+var gtmpstr = document.location.href;
+var g_baseURL = gtmpstr.substr(0, gtmpstr.lastIndexOf("/") ) + "/" + "webqtl_demo2.ppt_files";
+var g_showoutline = 1;
+var g_shownotes = includeNotes;
+var g_currentSlide = INITSLIDENUM, g_prevSlide = INITSLIDENUM;
+var saveFSSlideNum = saveTPSlideNum = g_currentSlide;
+var saveFSprevSlide = saveTPprevSlide = g_prevSlide;
+var g_slideType="ie";
+var appVer = navigator.appVersion;
+var msie = appVer.indexOf( "MSIE " ) + appVer.indexOf( "Internet Explorer " );
+var isnav = ( navigator.appName.indexOf( "Netscape" ) >= 0 );
+var msieWin31 = (appVer.indexOf( "Windows 3.1" ) > 0);
+var ver = 0;
+var g_done = 0;
+var g_prevotlobjidx = 0;
+var g_ShowFSDefault = 0;
+var g_lastVisibleSld = 1;
+var g_allHidden = false;
+function IsIE() {
+ return (msie >= 0 );
+}
+
+function IsNav() {
+ return (isnav);
+}
+var msiePos = appVer.indexOf( "MSIE " );
+var inexPos = appVer.indexOf( "Internet Explorer " );
+if ( msiePos >= 0 )
+ ver = parseFloat( appVer.substring( msiePos+5, appVer.indexOf ( ";", msiePos ) ) );
+else if( inexPos >= 0 )
+ ver=parseFloat( appVer.substring( inexPos+18, appVer.indexOf(";",inexPos) ) )
+else
+ ver = parseInt( appVer );
+
+//var g_supportsPPTHTML = 0; //!msieWin31 && ( ( msie >= 0 && ver >= 3.02 ) || ( msie < 0 && ver >= 3 ) );
+
+function GetCurrentSlideNum()
+{
+ obj = GetHrefObj( g_currentSlide );
+ if ( GetHrefObj( g_currentSlide ).m_origVisibility == 1 )
+ return obj.m_slideIdx;
+ else
+ return g_currentSlide;
+}
+
+function GetNumSlides()
+{
+ if ( GetHrefObj( g_currentSlide ).m_origVisibility == 1 )
+ return NUM_VISIBLE_SLIDES;
+ else
+ return g_docTable.length;
+}
+
+function GetHrefObj( slideIdx )
+{ return g_docTable[slideIdx - 1];
+}
+
+function GetSlideNum( slideHref )
+{
+ for (ii=0; ii<g_docTable.length; ii++) {
+ if ( g_docTable[ii].m_slideHref == slideHref )
+ return ii+1;
+ }
+ return 1;
+}
+
+function GoToNextSld()
+{
+ targetIdx = g_currentSlide + 1;
+ if ( GetHrefObj( targetIdx-1 ).m_origVisibility == 0 ) {
+ if ( targetIdx<=g_docTable.length ) {
+ obj = GetHrefObj( targetIdx );
+ obj.m_visibility = 1;
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0001.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0001.htm
new file mode 100755
index 00000000..11938baa
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0001.htm
@@ -0,0 +1,25 @@
+<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content="Aug-15-05: The GeneNetwork and WebQTL : PART 2 link to www.genenetwork.org"> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
+gId="slide0001.htm"
+if ( !parent.base.g_done && (parent.base.msie < 0 ) ) {
+ parent.base.g_done = 1;
+ document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref);
+}
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+if( !IsSldOrNts() ) return;
+if( MakeNotesVis() ) return;
+LoadSld( gId );
+playList();MakeSldVis(1);
+//-->
+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:3.53%;left:2.11%;width:96.15%;height:18.19%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-277 0 -1";mso-margin-left-alt:232; mso-text-indent-alt:0;position:absolute;top:11.65%;left:.96%;width:98.2%; height:73.78%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:73%'>The GeneNetwork and WebQTL : PART 2<span style="mso-spacerun: yes">&nbsp;&nbsp; </span><br> </span></span><span style='position:absolute;top:48.68%;left:4.34%;width:95.23%'><span style='font-size:73%'>link to www.genenetwork.org</span><span style='font-size: 73%;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <div style='position:absolute;top:24.02%;left:5.82%;width:54.03%;height:68.55%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='position:absolute;top:0%;left:3.67%;width:96.32%; height:61.34%'> <div style='mso-line-spacing:"-220 0 -216";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:0%;width:100.0%;height:51.68%'><span style='position:absolute;top:0%;left:0%;width:89.05%'><span style='mso-special-format: nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts";font-size: 99%'>l</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>Part 1. How to study </span></span><span style='position:absolute;top:23.57%; left:9.66%;width:79.13%'><span style='font-size:94%;color:#E9EB5D;mso-color-index: 3'>expression variation </span></span><span style='position:absolute; top:47.15%;left:9.66%;width:90.33%'><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>and genetic correlation </span></span><span style='position:absolute;top:70.73%;left:9.66%;width:79.13%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>(slides 2&#8211;17)<br> </span></span></div> <div style='mso-line-spacing:"-220 0 -216";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:60.5%;left:0%;width:93.63%;height:39.91%'><span style='position:absolute;top:0%;left:0%;width:95.1%'><span style='mso-special-format: nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts";font-size: 99%'>l</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>Part 2. Discovering </span></span><span style='position:absolute;top:30.52%; left:10.32%;width:89.67%'><span style='font-size:94%;color:#E9EB5D;mso-color-index: 3'>upstream modulators </span></span><span style='position:absolute;top:61.05%; left:10.32%;width:84.51%'><span style='font-size:94%;color:#E9EB5D;mso-color-index: 3'>(slides 18&#8211;29)</span></span></div> </div> </div> <img border=0 src="slide0001_image001.png" style='position:absolute;top:17.84%; left:74.17%;width:23.57%;height:64.31%'> <div class=O style='position:absolute;top:75.44%;left:74.56%;width:10.46%; height:14.48%'> <div style='mso-line-spacing:"-346 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:42.68%'><span style='font-family:"Arial Rounded MT Bold";font-size:233%;color:#6E6E6E'>RNA</span><span style='font-family:"Arial Rounded MT Bold";font-size:300%;color:#6E6E6E; display:none'><br> </span></div> <div style='mso-line-spacing:"-346 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:45.12%;left:0%;width:82.27%;height:54.87%'><span style='font-family:"Arial Rounded MT Bold";font-size:300%;color:#6E6E6E; mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:82.15%;left:4.1%;width:96.15%;height:18.19%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'></div> <img border=0 src="slide0001_image002.png" style='position:absolute;top:50.53%; left:48.74%;width:25.56%;height:13.07%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Part 2: Discovering upstream modulators and quantitative trait loci (QTLs). A quantitative trait locus is a chromosomal region that harbors one or a few polymorphic gene loci that influence a trait. We are going to be looking for QTLs that modulate the steady state expression level of App in the adult mouse forebrain.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0001_image001.png b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0001_image001.png
new file mode 100755
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Part 2: Discovering upstream modulators and quantitative trait loci (QTLs). A quantitative trait locus is a chromosomal region that harbors one or a few polymorphic gene loci that influence a trait. We are going to be looking for QTLs that modulate the steady state expression level of App in the adult mouse forebrain.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0002.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0002.htm
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+<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Aug-15-05> <link rel=Stylesheet href="master05_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
+gId="slide0002.htm"
+if ( !parent.base.g_done && (parent.base.msie < 0 ) ) {
+ parent.base.g_done = 1;
+ document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref);
+}
+if( !IsNts() ) Redirect( "PPTSld", gId );
+var g_hasTrans = true, g_autoTrans = false, g_transSecs = 1;
+//-->
+</script><script for=window event=onload language=JavaScript><!--
+if( !IsSldOrNts() ) return;
+if( MakeNotesVis() ) return;
+LoadSld( gId );
+playList();MakeSldVis(1);
+//-->
+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master05_background.png" v:shapes="_x0000_s3075" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div class=O style='position:absolute;top:92.93%;left:8.74%;width:18.67%; height:5.83%'> <div style='mso-line-spacing:"-134 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.48%'><span style='font-family:Times;font-size:117%;color:white;display:none'><br> </span></div> <div style='mso-line-spacing:"-134 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.51%;left:0%;width:100.0%;height:48.48%'><span style='font-family:Times;font-size:117%;color:white;mso-special-format:lastCR; display:none'><br> </span></div> </div> <div class=O style='position:absolute;top:92.93%;left:35.49%;width:29.4%; height:5.83%'> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.48%'><span style='font-family:Times;font-size:117%;color:white; display:none'><br> </span></div> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:51.51%;left:0%;width:100.0%; height:48.48%'><span style='font-family:Times;font-size:117%;color:white; mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:2.12%;left:-5.43%;width:105.96%;height:11.13%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:0%;left:0%;width:100.0%;height:92.06%'> <div style='text-align:center;mso-line-spacing:"-240 0 0";mso-margin-left-alt: 16;mso-text-indent-alt:16;position:absolute;top:0%;left:0%;width:100.0%; height:65.51%'><span style='font-family:Arial;font-size:267%;color:#E9EB5D'>How to make recombinant inbred strains (RI)</span><span style='font-family:Arial; font-size:150%;color:aqua;display:none'><br> </span></div> <div style='text-align:center;mso-line-spacing:"-160 0 0";mso-margin-left-alt: 16;mso-text-indent-alt:16;position:absolute;top:62.06%;left:6.37%;width:87.37%; height:37.93%'><span style='font-family:Arial;font-size:150%;color:aqua; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0002_image003.png" style='position:absolute;top:28.79%; left:45.43%;width:18.14%;height:1.06%'><img border=0 src="slide0002_image004.png" style='position:absolute;top:20.31%;left:28.21%; width:1.98%;height:11.83%'> <div style='position:absolute;top:15.01%;left:25.03%;width:19.47%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.09%;left:6.8%;width:93.19%; height:81.81%'> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>C57BL/6J (B)<br> </span></div> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:83.21%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <img border=0 src="slide0002_image005.png" style='position:absolute;top:20.31%; left:30.46%;width:1.98%;height:11.83%'> <div style='position:absolute;top:15.01%;left:62.25%;width:16.55%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.09%;left:8.0%;width:92.8%; height:81.81%'> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>DBA/2J (D)<br> </span></div> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:82.75%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <img border=0 src="slide0002_image006.png" style='position:absolute;top:20.31%; left:66.62%;width:4.23%;height:11.83%'><img border=0 src="slide0002_image007.png" style='position:absolute;top:35.51%;left:23.97%; width:50.86%;height:1.23%'><img border=0 src="slide0002_image008.png" style='position:absolute;top:35.68%;left:24.1%;width:.66%;height:4.06%'><img border=0 src="slide0002_image009.png" style='position:absolute;top:35.68%; left:40.92%;width:.52%;height:4.06%'><img border=0 src="slide0002_image010.png" style='position:absolute;top:35.68%;left:57.61%;width:.52%;height:4.06%'><img border=0 src="slide0002_image011.png" style='position:absolute;top:35.68%; left:74.3%;width:.52%;height:3.88%'> <div style='position:absolute;top:42.75%;left:5.69%;width:6.49%;height:12.36%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.57%;left:20.4%;width:73.46%; height:84.28%'> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F1<br> </b></span></div> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.84%;left:0%;width:83.33%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format:lastCR; display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0002_image012.png" style='position:absolute;top:39.92%; left:22.25%;width:4.23%;height:11.83%'><img border=0 src="slide0002_image013.png" style='position:absolute;top:39.92%;left:38.94%; width:4.23%;height:11.83%'><img border=0 src="slide0002_image014.png" style='position:absolute;top:39.92%;left:55.62%;width:4.23%;height:11.83%'><img border=0 src="slide0002_image015.png" style='position:absolute;top:39.39%; left:72.84%;width:4.23%;height:11.83%'><img border=0 src="slide0002_image016.png" style='position:absolute;top:43.46%;left:43.97%; width:11.12%;height:3.53%'><img border=0 src="slide0002_image017.png" style='position:absolute;top:60.95%;left:20.66%;width:9.8%;height:3.53%'> <div style='position:absolute;top:71.73%;left:58.41%;width:20.26%;height:26.67%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:12.58%;left:6.53%;width:88.23%; height:83.44%'> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:69.84%'><span style='position:absolute;top:0%;left:0%;width:99.25%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'><b>20 generations </b></span></span><span style='position:absolute;top:39.77%;left:0%;width:100.0%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'><b>brother-sister </b></span></span><span style='position:absolute;top:80.68%;left:0%;width:99.25%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'><b>matings</b></span><span style='font-family:Arial;font-size:233%;color:#FFFF99;display:none'><b><br> </b></span></span></div> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:73.8%;left:0%;width:99.25%; height:26.98%'><span style='font-family:Arial;font-size:233%;color:#FFFF99; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0002_image018.png" style='position:absolute;top:60.95%; left:50.72%;width:9.93%;height:3.53%'><img border=0 src="slide0002_image019.png" style='position:absolute;top:62.72%;left:54.03%;width:4.37%;height:28.97%'><img border=0 src="slide0002_image020.png" style='position:absolute;top:61.13%; left:79.86%;width:10.86%;height:3.53%'><img border=0 src="slide0002_image021.png" style='position:absolute;top:62.36%;left:83.97%; width:4.1%;height:28.79%'> <div style='position:absolute;top:92.93%;left:21.72%;width:9.27%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.09%;left:12.85%;width:85.71%; height:81.81%'> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD1</span><span style='font-family:Arial;font-size:150%;color:#484848;display:none'><br> </span></div> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:83.33%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#484848;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:92.75%;left:51.78%;width:9.27%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.09%;left:12.85%;width:85.71%; height:81.81%'> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD2<br> </span></div> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:83.33%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:92.93%;left:80.79%;width:10.99%;height:5.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:15.62%;left:12.04%;width:87.95%; height:68.75%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD80</span></div> </div> <div style='position:absolute;top:90.45%;left:64.1%;width:17.74%;height:17.49%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:6.06%;left:7.46%;width:92.53%; height:88.88%'> <div style='mso-line-spacing:"-341 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.86%'><span style='font-family:Arial;font-size:300%;color:#FFFF99'>+ É +<br> </span></div> <div style='mso-line-spacing:"-341 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.0%;left:0%;width:83.87%;height:48.86%'><span style='font-family:Arial;font-size:300%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:62.89%;left:4.9%;width:6.49%;height:12.36%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:7.14%;left:20.4%;width:73.46%; height:84.28%'> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F2<br> </b></span></div> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.84%;left:0%;width:83.33%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format:lastCR; display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0002_image022.png" style='position:absolute;top:54.24%; left:18.01%;width:75.62%;height:1.06%'><img border=0 src="slide0002_image023.png" style='position:absolute;top:54.06%;left:17.88%; width:.66%;height:3.88%'><img border=0 src="slide0002_image024.png" style='position:absolute;top:54.24%;left:32.45%;width:.52%;height:3.88%'><img border=0 src="slide0002_image025.png" style='position:absolute;top:54.24%; left:48.07%;width:.52%;height:4.06%'><img border=0 src="slide0002_image026.png" style='position:absolute;top:54.24%;left:62.78%;width:.52%;height:4.06%'><img border=0 src="slide0002_image027.png" style='position:absolute;top:54.24%; left:77.74%;width:.52%;height:4.06%'><img border=0 src="slide0002_image028.png" style='position:absolute;top:54.59%;left:92.71%;width:.52%;height:3.71%'><img border=0 src="slide0002_image029.png" style='position:absolute;top:44.87%; left:49.13%;width:.79%;height:10.07%'><img border=0 src="slide0002_image030.png" style='position:absolute;top:57.59%;left:15.89%;width:4.23%;height:11.83%'><img border=0 src="slide0002_image031.png" style='position:absolute;top:57.59%; left:30.86%;width:4.37%;height:11.83%'><img border=0 src="slide0002_image032.png" style='position:absolute;top:57.59%;left:46.35%; width:4.23%;height:11.83%'><img border=0 src="slide0002_image033.png" style='position:absolute;top:57.59%;left:60.92%;width:4.23%;height:11.83%'><img border=0 src="slide0002_image034.png" style='position:absolute;top:57.59%; left:75.62%;width:4.23%;height:11.83%'><img border=0 src="slide0002_image035.png" style='position:absolute;top:57.59%;left:90.86%; width:4.23%;height:11.83%'><img border=0 src="slide0002_image036.png" style='position:absolute;top:84.09%;left:56.42%;width:1.98%;height:1.41%'><img border=0 src="slide0002_image037.png" style='position:absolute;top:80.21%; left:25.96%;width:1.98%;height:11.83%'><img border=0 src="slide0002_image038.png" style='position:absolute;top:84.98%;left:86.09%; width:1.85%;height:2.12%'> <div style='position:absolute;top:75.61%;left:1.58%;width:13.9%;height:13.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:7.69%;left:.95%;width:99.04%; height:85.89%'> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:7.69%;width:83.65%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'><b>BXD RI<br> </b></span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:32.83%;left:0%;width:100.0%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'><b>Strain set<br> </b></span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:67.16%;left:7.69%;width:83.65%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0002_image039.png" style='position:absolute;top:12.36%; left:14.17%;width:10.59%;height:6.36%'><img border=0 src="slide0002_image040.png" style='position:absolute;top:10.6%;left:76.42%; width:14.3%;height:8.3%'> <div style='position:absolute;top:20.84%;left:17.48%;width:9.53%;height:13.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:7.69%;left:6.94%;width:88.88%; height:85.89%'> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:7.81%;width:82.81%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>fully<br> </span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:32.83%;left:0%;width:100.0%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>inbred<br> </span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:67.16%;left:7.81%;width:82.81%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:38.16%;left:2.91%;width:11.78%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.09%;left:3.37%;width:94.38%; height:81.81%'> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>isogenic<br> </span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:51.11%;left:8.33%;width:83.33%; height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:54.41%;left:1.85%;width:12.18%;height:13.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:7.69%;left:2.17%;width:95.65%; height:85.89%'> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:7.95%;width:84.09%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>hetero-<br> </span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:32.83%;left:0%;width:100.0%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>geneous<br> </span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:67.16%;left:7.95%;width:84.09%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:74.91%;left:29.0%;width:21.98%;height:21.73%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:4.06%;left:5.42%;width:89.15%; height:91.86%'> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:79.64%'><span style='position:absolute;top:0%;left:.67%;width:99.32%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>Recombined </span></span><span style='position:absolute;top:25.55%;left:.67%;width:99.32%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>chromosomes </span></span><span style='position:absolute;top:51.11%;left:0%;width:100.0%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>are needed for </span></span><span style='position:absolute;top:75.55%;left:.67%;width:99.32%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>mapping<br> </span></span></div> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:80.53%;left:.67%;width:99.32%; height:19.46%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:10.24%;left:25.56%;width:10.46%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.09%;left:12.65%;width:87.34%; height:81.81%'> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>female<br> </span></div> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:84.05%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:10.24%;left:64.5%;width:7.94%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.09%;left:15.0%;width:83.33%; height:81.81%'> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>male<br> </span></div> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:84.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:30.56%;left:78.01%;width:19.47%;height:9.71%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:16.36%;left:6.12%;width:93.19%; height:74.54%'> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:41.46%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'>chromosome pair</span><span style='font-family:Arial;font-size:150%;color:#FFFF99;display:none'><br> </span></div> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:46.34%;left:0%;width:83.21%;height:53.65%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <img border=0 src="slide0002_image041.png" style='position:absolute;top:31.09%; left:65.82%;width:12.84%;height:2.82%'> <div style='position:absolute;top:86.21%;left:3.97%;width:9.8%;height:14.13%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:5.0%;left:5.4%;width:90.54%; height:87.5%'> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:8.95%;width:82.08%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:#E9EB5D'>Inbred<br> </span></div> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:25.71%;left:0%;width:100.0%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:#E9EB5D'>Isogenic<br> </span></div> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:50.0%;left:4.47%;width:89.55%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:#E9EB5D'>siblings</span><span style='font-family:Arial;font-size:117%;color:aqua;display:none'><br> </span></div> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:75.71%;left:8.95%;width:82.08%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:aqua; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0002_image042.png" style='position:absolute;top:79.32%; left:83.84%;width:1.98%;height:11.83%'><img border=0 src="slide0002_image043.png" style='position:absolute;top:84.8%;left:83.7%; width:1.85%;height:2.12%'><img border=0 src="slide0002_image044.png" style='position:absolute;top:79.85%;left:53.5%;width:1.98%;height:11.83%'><img border=0 src="slide0002_image045.png" style='position:absolute;top:83.92%; left:53.5%;width:1.98%;height:1.41%'><img border=0 src="slide0002_image046.png" style='position:absolute;top:80.21%;left:23.44%;width:1.98%;height:11.83%'> <div style='position:absolute;top:18.72%;left:43.04%;width:13.77%;height:9.54%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='mso-line-spacing:"-341 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:11.11%;left:9.61%;width:90.38%; height:79.62%'><span style='font-family:Arial;font-size:300%;color:aqua'>B</span><span style='font-family:Arial;font-size:300%;color:#FFFF99'>X</span><span style='font-family:Arial;font-size:300%;color:#FF1911'>D</span></div> </div> <img border=0 src="slide0002_image047.png" style='position:absolute;top:27.2%; left:32.98%;width:31.39%;height:4.06%'><img border=0 src="slide0002_image048.png" style='position:absolute;top:66.25%;left:60.92%; width:1.98%;height:3.18%'><img border=0 src="slide0002_image049.png" style='position:absolute;top:64.31%;left:15.89%;width:1.98%;height:5.3%'><img border=0 src="slide0002_image050.png" style='position:absolute;top:57.59%; left:75.62%;width:1.98%;height:5.65%'><img border=0 src="slide0002_image051.png" style='position:absolute;top:28.44%;left:48.87%;width:.92%;height:8.12%'><img border=0 src="slide0002_image052.png" style='position:absolute;top:62.19%; left:23.84%;width:3.57%;height:16.96%'><img border=0 src="slide0002_image053.png" style='position:absolute;top:61.48%;left:63.17%; width:1.98%;height:8.12%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The next few slides provide a short introduction to mapping the loci that are responsible for variation in a trait such as App expression level. These modulatory regions of the genome are sometimes called quantitative trait loci or QTLs. You may want to do some independent reading on this topic if this is your first exposure to QTL analysis.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The genetic reference population (GRP) of BXD recombinant inbred strains were originally generated about 25 years ago by Benjamin Taylor at The Jackson Laboratory. He crossed female C57BL/6J mice with male DBA/2J mice to generate the F1 and F2 progeny. At the bottom of this slide we have schematized one chromosome pair from three of the BXD RI strains.<span style="mso-spacerun: yes">&nbsp; </span>The dashed vertical lines that lead to the final BXD RI lines involve 21 full sib matings (about 7 years of breeding). Some lines die out during inbreeding. For example, there is no longer any BXD3 strain.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. Over the last decade, our group (Lu Lu and Rob Williams) and Jeremy Peirce and Lee Silver at Princeton have enlarged Ben TaylorÕs set. There are now just over 80 BXD strains. They have all been genotyped using about 13,700 markers (SNPs and microsatellites). These markers are used to define the ÒblueÓ and ÒredÓ regions of the chromosomes as shown in the figure above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. Chromosomes of RI GRPs usually have about 4 times as many recombinations as those of F2 animals. However, unlike an F2, both chromosomes of an RI are identical. Therefore, 50 RI strains contain as many recombinations as 100 F2 animals.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. BXD43 through BXD100 were generated using a special method that resulted in a further doubling of the average recombination density per chromosome. The entire set of 80 BXDs therefore contains as many recombinations as about 260 F2 animals.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0002_notes_pane.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0002_notes_pane.htm
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The next few slides provide a short introduction to mapping the loci that are responsible for variation in a trait such as App expression level. These modulatory regions of the genome are sometimes called quantitative trait loci or QTLs. You may want to do some independent reading on this topic if this is your first exposure to QTL analysis.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The genetic reference population (GRP) of BXD recombinant inbred strains were originally generated about 25 years ago by Benjamin Taylor at The Jackson Laboratory. He crossed female C57BL/6J mice with male DBA/2J mice to generate the F1 and F2 progeny. At the bottom of this slide we have schematized one chromosome pair from three of the BXD RI strains.<span style="mso-spacerun: yes">&nbsp; </span>The dashed vertical lines that lead to the final BXD RI lines involve 21 full sib matings (about 7 years of breeding). Some lines die out during inbreeding. For example, there is no longer any BXD3 strain.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>1. Over the last decade, our group (Lu Lu and Rob Williams) and Jeremy Peirce and Lee Silver at Princeton have enlarged Ben TaylorÕs set. There are now just over 80 BXD strains. They have all been genotyped using about 13,700 markers (SNPs and microsatellites). These markers are used to define the ÒblueÓ and ÒredÓ regions of the chromosomes as shown in the figure above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. Chromosomes of RI GRPs usually have about 4 times as many recombinations as those of F2 animals. However, unlike an F2, both chromosomes of an RI are identical. Therefore, 50 RI strains contain as many recombinations as 100 F2 animals.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. BXD43 through BXD100 were generated using a special method that resulted in a further doubling of the average recombination density per chromosome. The entire set of 80 BXDs therefore contains as many recombinations as about 260 F2 animals.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'><img border=0 src="slide0003_image054.png" style='position:absolute;top:40.45%; left:73.24%;width:14.56%;height:5.3%'> <div class=O style='position:absolute;top:40.81%;left:74.56%;width:11.92%; height:7.24%'> <div style='mso-line-spacing:"-126 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white'>aa<br> </span></div> <div style='mso-line-spacing:"-126 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:41.46%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white;mso-special-format:lastCR; display:none'><br> </span></div> </div> <img border=0 src="slide0003_image055.png" style='position:absolute;top:16.6%; left:73.24%;width:14.56%;height:5.3%'> <div class=O style='position:absolute;top:16.96%;left:74.56%;width:11.92%; height:7.24%'> <div style='mso-line-spacing:"-126 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white'>aaaa<br> </span></div> <div style='mso-line-spacing:"-126 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:41.46%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white;mso-special-format:lastCR; display:none'><br> </span></div> </div> <div style='position:absolute;top:20.31%;left:71.65%;width:25.96%;height:19.43%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:10.0%;left:5.1%;width:95.4%; height:84.54%'> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:#E2EBF0'>D2 strain</span><span style='font-family: Palatino;font-size:333%;color:#FBFDB8;display:none'><br> </span></div> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:49.46%;left:0%;width:88.77%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#FBFDB8;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:43.81%;left:71.65%;width:25.56%;height:19.43%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:10.9%;left:5.18%;width:94.81%; height:84.54%'> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:#F6BF69'>B6 strain</span><span style='font-family: Palatino;font-size:333%;color:#F6BF69;display:none'><br> </span></div> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:48.38%;left:0%;width:87.43%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#F6BF69;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0003_image056.png" style='position:absolute;top:17.31%; left:31.25%;width:1.32%;height:42.22%'><img border=0 src="slide0003_image057.png" style='position:absolute;top:40.63%;left:31.39%; width:39.86%;height:3.53%'> <div style='position:absolute;top:20.67%;left:38.27%;width:30.19%;height:26.32%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:6.04%;left:4.38%;width:92.1%; height:89.93%'> <div style='mso-line-spacing:"-320 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:58.2%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size: 233%;color:#E9EB5D'><i>amount of </i></span></span><span style='position:absolute; top:53.84%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'><i>transcript</i></span><span style='font-family:Palatino;font-size:367%;color:#E9EB5D;display:none'><br> </span></span></div> <div style='mso-line-spacing:"-422 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:61.19%;left:0%;width:100.0%;height:38.8%'><span style='font-family:Palatino;font-size:367%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:6.0%;left:72.45%;width:21.05%;height:19.43%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:10.0%;left:6.28%;width:88.67%; height:84.54%'> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:white'>4 </span><span style='font-size:233%; color:white'>units</span><span style='font-family:Palatino;font-size:333%; color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:49.46%;left:0%;width:100.0%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:29.5%;left:72.45%;width:21.05%;height:19.43%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:10.9%;left:6.28%;width:88.67%; height:84.54%'> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:white'>2</span><span style='font-size:233%; color:white'> units</span><span style='font-family:Palatino;font-size:333%; color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:48.38%;left:0%;width:100.0%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0003_image058.png" style='position:absolute;top:15.72%; left:31.25%;width:40.26%;height:5.47%'> <div class=O style='position:absolute;top:17.84%;left:9.93%;width:4.76%; height:16.43%'> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:83.33%;height:50.53%'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div class=O style='position:absolute;top:17.84%;left:17.88%;width:4.23%; height:16.43%'> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:84.37%;height:50.53%'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:79.5%;left:5.43%;width:104.1%;height:13.6%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='mso-line-spacing:"-256 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:3.89%;left:1.27%;width:98.72%; height:93.5%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:233%;color:#E2EBF0'>D</span><span style='font-size:233%; color:#E9EB5D'> </span><span style='font-size:200%;color:#E9EB5D'>and </span><span style='font-size:233%;color:#F6BF69'>B</span><span style='font-size:233%; color:#E9EB5D'> </span><span style='font-size:200%;color:#E9EB5D'>may be SNP-like variants in the promoter </span></span><span style='position:absolute; top:34.72%;left:0%;width:99.74%'><span style='font-size:200%;color:#E9EB5D'>itself (cis QTL) or in </span><span style='font-size:200%;color:#EAEC5E'>upstream genes (trans QTLs)</span><span style='font-size:300%;color:#E9EB5D'>.</span></span></div> </div> <div class=O style='position:absolute;top:7.59%;left:4.23%;width:22.78%; height:16.25%'> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:1.74%;width:97.09%; height:33.69%'><span style='font-size:200%;color:#E9EB5D'>UPSTREAM<br> </span></div> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:32.6%;left:0%;width:100.0%; height:33.69%'><span style='font-size:200%;color:#E9EB5D'>modulators<br> </span></div> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:66.3%;left:4.65%;width:90.69%; height:33.69%'><span style='font-size:200%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:7.59%;left:44.5%;width:16.95%;height:17.84%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:3.96%;left:7.03%;width:85.15%; height:92.07%'> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0'>High<br> </span></div> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:49.46%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:63.78%;left:8.74%;width:6.35%;height:17.84%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:3.96%;left:20.83%;width:75.0%; height:92.07%'> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:83.33%;height:50.53%'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:63.78%;left:15.09%;width:5.96%;height:17.84%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:3.96%;left:20.0%;width:71.11%; height:92.07%'> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:84.37%;height:50.53%'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:71.02%;left:7.54%;width:16.02%;height:12.54%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:5.63%;left:7.43%;width:91.73%; height:87.32%'> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.0%'><span style='font-size:200%;color:#E9EB5D'><i>cis QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:48.38%;left:0%;width:82.88%;height:50.0%'><span style='font-size:200%;color:#E9EB5D;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0003_image059.png" style='position:absolute;top:57.24%; left:-1.05%;width:101.98%;height:7.59%'> <div class=O style='position:absolute;top:44.16%;left:47.15%;width:9.4%; height:12.72%'> <div style='mso-line-spacing:"-272 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:51.38%'><span style='font-size:233%;color:#F6BF69'>Low<br> </span></div> <div style='mso-line-spacing:"-272 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.0%;left:0%;width:83.09%;height:51.38%'><span style='font-size:233%;color:#F6BF69;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:58.12%;left:.79%;width:113.37%;height:6.36%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='mso-line-spacing:"-200 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:11.11%;left:1.16%;width:98.83%; height:75.0%'><span style='font-family:Gadget;font-size:167%;color:white'>&gt;&gt;&gt;&gt;PROMOTER--ATG-Exon1-Intron1-Exon2-Intron2 - etc-3'UTR &gt;&gt;&gt;&gt;&gt;</span></div> </div> <img border=0 src="slide0003_image060.png" style='position:absolute;top:37.98%; left:8.6%;width:13.77%;height:20.67%'> <div class=O style='position:absolute;top:40.28%;left:10.99%;width:1.72%; height:11.48%'> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:46.15%'><span style='font-family:"\30D2\30E9\30AE\30CE\89D2\30B4 Pro W3"; font-size:233%;color:white'><b>&#4;</b></span><span style='font-family:Palatino; font-size:233%;color:white;display:none'><b><br> </b></span></div> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:50.76%;left:7.69%;width:84.61%; height:50.76%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <div class=O style='position:absolute;top:40.63%;left:18.41%;width:1.72%; height:11.48%'> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:46.15%'><span style='font-family:"\30D2\30E9\30AE\30CE\89D2\30B4 Pro W3"; font-size:233%;color:white'><b>&#4;</b></span><span style='font-family:Palatino; font-size:233%;color:white;display:none'><b><br> </b></span></div> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:49.23%;left:7.69%;width:84.61%; height:50.76%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <img border=0 src="slide0003_image061.png" style='position:absolute;top:33.74%; left:8.6%;width:13.77%;height:20.67%'> <div class=O style='position:absolute;top:36.39%;left:18.41%;width:1.72%; height:11.48%'> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:46.15%'><span style='font-family:"\30D2\30E9\30AE\30CE\89D2\30B4 Pro W3"; font-size:233%;color:white'><b>&#4;</b></span><span style='font-family:Palatino; font-size:233%;color:white;display:none'><b><br> </b></span></div> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:49.23%;left:7.69%;width:84.61%; height:50.76%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <div class=O style='position:absolute;top:36.04%;left:10.99%;width:1.72%; height:11.48%'> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:46.15%'><span style='font-family:"\30D2\30E9\30AE\30CE\89D2\30B4 Pro W3"; font-size:233%;color:white'><b>&#4;</b></span><span style='font-family:Palatino; font-size:233%;color:white;display:none'><b><br> </b></span></div> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:49.23%;left:7.69%;width:84.61%; height:50.76%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <div class=O style='position:absolute;top:37.8%;left:20.66%;width:.13%; height:12.01%'> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; display:none'><b><br> </b></span></div> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:51.47%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <img border=0 src="slide0003_image062.png" style='position:absolute;top:27.38%; left:4.9%;width:21.45%;height:21.37%'> <div style='position:absolute;top:27.38%;left:5.03%;width:20.92%;height:12.54%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:5.63%;left:6.32%;width:94.3%; height:87.32%'> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.0%'><span style='font-size:200%;color:#E9EB5D'><i>trans QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:48.38%;left:0%;width:83.22%;height:50.0%'><span style='font-size:200%;color:#E9EB5D;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>This slide is illustrates two major types of QTLs that modulate variability in transcript-relative steady state abundance.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>1. cis QTLs are defined as QTLs that are closely linked to the gene whose transcript is the measured trait. For example, a polymorphism in the promoter that affects binding of a transcription factor. However, cis QTLs can be far upstream or downstream polymorphisms in enhancers or may be in 3Õ UTR binding sites that affect message stability.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. trans QTLs map far enough away from the location of the gene that gives rise to the transcript that is being measured so that we can be fairly certain that the QTL is not in the gene itself. The most blatant type of trans QTL would be a polymorphism in a transcription factor. But in the majority of cases, the trans QTLs can be far removed in a mechanistic sense from the actual events modulating transcript abundance. That is why there are three overlapping arrows in the figure.<span style="mso-spacerun: yes">&nbsp; </span>The way in which an upstream polymorphism influences a downstream difference in mRNA abundance can be indirect. Effects can:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>a.<span style="mso-spacerun: yes">&nbsp; </span>cross tissue types (a polymorphic liver enzyme may affect CNS gene expression)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>b.<span style="mso-spacerun: yes">&nbsp; </span>cross time (the modulator is only expressed for one day during development but has permanent effects in adults)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>c.<span style="mso-spacerun: yes">&nbsp; </span>may be contingent on environmental factors (heat shock may trigger the expression of a polymorphic factor that affects mRNA abundance).</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>This slide is illustrates two major types of QTLs that modulate variability in transcript-relative steady state abundance.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>1. cis QTLs are defined as QTLs that are closely linked to the gene whose transcript is the measured trait. For example, a polymorphism in the promoter that affects binding of a transcription factor. However, cis QTLs can be far upstream or downstream polymorphisms in enhancers or may be in 3Õ UTR binding sites that affect message stability.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>2. trans QTLs map far enough away from the location of the gene that gives rise to the transcript that is being measured so that we can be fairly certain that the QTL is not in the gene itself. The most blatant type of trans QTL would be a polymorphism in a transcription factor. But in the majority of cases, the trans QTLs can be far removed in a mechanistic sense from the actual events modulating transcript abundance. That is why there are three overlapping arrows in the figure.<span style="mso-spacerun: yes">&nbsp; </span>The way in which an upstream polymorphism influences a downstream difference in mRNA abundance can be indirect. Effects can:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>a.<span style="mso-spacerun: yes">&nbsp; </span>cross tissue types (a polymorphic liver enzyme may affect CNS gene expression)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>b.<span style="mso-spacerun: yes">&nbsp; </span>cross time (the modulator is only expressed for one day during development but has permanent effects in adults)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>c.<span style="mso-spacerun: yes">&nbsp; </span>may be contingent on environmental factors (heat shock may trigger the expression of a polymorphic factor that affects mRNA abundance).</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'><img border=0 src="slide0004_image063.png" style='position:absolute;top:38.86%; left:5.03%;width:84.9%;height:45.75%'> <div style='position:absolute;top:5.47%;left:2.51%;width:93.64%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";position:absolute;top:20.4%; left:1.41%;width:97.31%;height:75.51%'><span style='font-family:Verdana; font-size:64%'>Discovering upstream modulatory loci</span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </div> <img border=0 src="slide0004_image064.png" style='position:absolute;top:20.31%; left:18.01%;width:19.2%;height:25.97%'><img border=0 src="slide0004_image065.png" style='position:absolute;top:32.5%;left:78.01%; width:19.2%;height:25.97%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Please bring the Trait Data and Analysis window to the front and look for the Interval Mapping button. Confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Mapping Scale is set to GENETIC</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Permutation test YES<span style="mso-spacerun: yes">&nbsp; </span>(2000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Bootstrap test YES (2000 is the default number)</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+</script> </body> </html> \ No newline at end of file
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Please bring the Trait Data and Analysis window to the front and look for the Interval Mapping button. Confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Select Mapping Scale is set to GENETIC</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Options: Permutation test YES<span style="mso-spacerun: yes">&nbsp; </span>(2000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Options: Bootstrap test YES (2000 is the default number)</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0005.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0005.htm
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+<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+</script> <meta name=Description content="Aug-15-05: WebQTL searches for upstream controllers"> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
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+</script><script for=window event=onload language=JavaScript><!--
+if( !IsSldOrNts() ) return;
+if( MakeNotesVis() ) return;
+LoadSld( gId );
+playList();MakeSldVis(1);
+//-->
+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-.53%;left:1.72%;width:101.19%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:18.36%;left:.91%;width:98.95%; height:75.51%'><span style='font-family:Verdana;font-size:64%'><i>WebQTL searches for upstream controllers</i></span><span style='font-family:Verdana; font-size:73%;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </div> <div style='position:absolute;top:71.02%;left:34.96%;width:45.96%;height:19.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:3.57%;left:2.3%;width:97.69%; height:91.96%'><span style='position:absolute;top:0%;left:0%;width:98.82%'><span style='font-size:167%;color:#E9EB5D'><i>App maps on Chr 16 (blue </i></span></span><span style='position:absolute;top:25.24%;left:0%;width:100.0%'><span style='font-size:167%;color:#E9EB5D'><i>arrow points to the orange </i></span></span><span style='position:absolute;top:49.51%;left:0%;width:86.72%'><span style='font-size:167%;color:#E9EB5D'><i>triangle) but the best </i></span></span><span style='position:absolute;top:74.75%;left:0%;width:86.72%'><span style='font-size:167%;color:#E9EB5D'><i>locus is on Chr 7.</i></span><span style='font-size:233%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <img border=0 src="slide0005_image066.png" style='position:absolute;top:8.83%; left:1.19%;width:98.27%;height:53.88%'><img border=0 src="slide0005_image067.png" style='position:absolute;top:46.64%;left:15.76%; width:15.89%;height:53.35%'><img border=0 src="slide0005_image068.png" style='position:absolute;top:46.64%;left:80.0%;width:10.99%;height:53.35%'><img border=0 src="slide0005_image069.png" style='position:absolute;top:41.34%; left:76.02%;width:11.65%;height:22.79%'><img border=0 src="slide0005_image070.png" style='position:absolute;top:41.51%;left:29.13%; width:12.71%;height:24.38%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This is a major output type: a so-called full-genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromosomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans at almost 0 megabases). The blue labels along the bottom of the figure list a subset of the 3795 markers that were used in mapping.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The thick blue wavy line running across chromosomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of all markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The red line and the red axis to the far right provide an estimate of the effect that a QTL has on expression of App (this estimate of the so-called additive effect tends to be too high). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher trait values. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and those that have the D/D genotype at a specific marker. For example, on distal Chr 7 the red line peaks at a value of about 0.2. That means that this region of chromosome 2 is responsible for a 0.4 unit expression difference between B/B strains and the D/D strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides a method to evaluate whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But using the bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 3 and Chr 7 under the LRS peaks. That is somewhat reassuring. But notice that a substantial number of bootstrap are scattered around on other chromosomes. About 30% of the bootstrap resamples have a peak on Chr 7. That is pretty good, but does makes us realize that the sample we are working with is still quite small and fragile.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The horizontal dashed lines at 10.5 and 17.3 are the likelihood ratio statistic (LRS) values associated with the suggestive and significant genome-wide probabilities that were established by permutations of phenotypes across genotypes. We shuffle randomly 2000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 17.3. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 3 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations. Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. If you click on the body of the map, say on the blue line, then you will generate a view on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. If you click on a marker symbol, then you will generate a new Trait data and Analysis window with the genotypes loaded into the window just like any other trait. More on this in Section 3.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4. You can drag these maps off of the browser window and onto your desktop. They will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5. There is also an option at the bottom of the page to download a 2X higher resolution image of this plot for papers and presentations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>6. You can also download the results of the analysis in a text format</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>This is a major output type: a so-called full-genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromosomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans at almost 0 megabases). The blue labels along the bottom of the figure list a subset of the 3795 markers that were used in mapping.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The thick blue wavy line running across chromosomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of all markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The red line and the red axis to the far right provide an estimate of the effect that a QTL has on expression of App (this estimate of the so-called additive effect tends to be too high). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher trait values. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and those that have the D/D genotype at a specific marker. For example, on distal Chr 7 the red line peaks at a value of about 0.2. That means that this region of chromosome 2 is responsible for a 0.4 unit expression difference between B/B strains and the D/D strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides a method to evaluate whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But using the bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 3 and Chr 7 under the LRS peaks. That is somewhat reassuring. But notice that a substantial number of bootstrap are scattered around on other chromosomes. About 30% of the bootstrap resamples have a peak on Chr 7. That is pretty good, but does makes us realize that the sample we are working with is still quite small and fragile.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The horizontal dashed lines at 10.5 and 17.3 are the likelihood ratio statistic (LRS) values associated with the suggestive and significant genome-wide probabilities that were established by permutations of phenotypes across genotypes. We shuffle randomly 2000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 17.3. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 3 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations. Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. If you click on the body of the map, say on the blue line, then you will generate a view on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. If you click on a marker symbol, then you will generate a new Trait data and Analysis window with the genotypes loaded into the window just like any other trait. More on this in Section 3.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>4. You can drag these maps off of the browser window and onto your desktop. They will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>5. There is also an option at the bottom of the page to download a 2X higher resolution image of this plot for papers and presentations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>6. You can also download the results of the analysis in a text format</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> <meta name=Description content="Aug-15-05: Genetic versus Physical maps for App expression"> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-.53%;left:1.72%;width:116.02%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:18.36%;left:.79%;width:99.2%; height:75.51%'><span style='font-family:Verdana;font-size:64%'><i>Genetic versus Physical maps for App expression</i></span><span style='font-family: Verdana;font-size:73%;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </div> <img border=0 src="slide0006_image071.png" style='position:absolute;top:8.83%; left:1.72%;width:98.27%;height:39.92%'><img border=0 src="slide0006_image072.png" style='position:absolute;top:35.51%;left:1.85%; width:98.27%;height:54.06%'> <div class=O style='position:absolute;top:91.69%;left:5.96%;width:95.23%; height:7.42%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:117%;color:#E9EB5D'><i>The difference between genetic and physical scale is analogous to measuring the </i></span></span><span style='position:absolute;top:42.85%;left:0%;width:96.66%'><span style='font-size:117%;color:#E9EB5D'><i>separation between New York and Boston in either travel hours or kilometers</i></span><span style='font-size:150%; color:#E9EB5D'><i>.</i></span><span style='font-size:167%;color:#E9EB5D; mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The map on the top has an X-axis scale based on frequency of recombinations events between markers (B to D transitions, see slide 19 for a color-coded example). These so-called genetic maps are scaled in centimorgan (recombinations per 100 gametes). In contrast, the physical map shown below the genetic map has an X-axis scale based on DNA length measured in nucleotides or base-pairs. Notice the large difference between the two maps in the size of Chr 19 (large on the genetic scale but small on the physical scale).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Also notice the large difference in the width of the chromosome 7 QTL peak. In mice, recombinations occur with higher frequency toward the telomeric side (right side) of each chromosome. As a result, genetic maps are stretched out more toward the telomere relative to a physical map. The QTL on distal Chr 7 is therefore actually more precisely mapped than might appear looking at the genetic map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The physical scale is becoming more useful than the genetic scale primarily because many other data types can be easily superimposed on a physical map. You will see more examples in the next several slides.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The map on the top has an X-axis scale based on frequency of recombinations events between markers (B to D transitions, see slide 19 for a color-coded example). These so-called genetic maps are scaled in centimorgan (recombinations per 100 gametes). In contrast, the physical map shown below the genetic map has an X-axis scale based on DNA length measured in nucleotides or base-pairs. Notice the large difference between the two maps in the size of Chr 19 (large on the genetic scale but small on the physical scale).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Also notice the large difference in the width of the chromosome 7 QTL peak. In mice, recombinations occur with higher frequency toward the telomeric side (right side) of each chromosome. As a result, genetic maps are stretched out more toward the telomere relative to a physical map. The QTL on distal Chr 7 is therefore actually more precisely mapped than might appear looking at the genetic map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The physical scale is becoming more useful than the genetic scale primarily because many other data types can be easily superimposed on a physical map. You will see more examples in the next several slides.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-.53%;left:1.72%;width:101.05%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:18.36%;left:.91%;width:97.9%; height:75.51%'><span style='font-family:Verdana;font-size:64%'>Physical map for distal chromosome 7</span><span style='font-family:Verdana;font-size:73%; mso-special-format:lastCR;display:none'><i><br> </i></span></div> </div> <div style='position:absolute;top:7.77%;left:5.82%;width:94.03%;height:6.18%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:11.42%;left:1.12%;width:98.87%; height:74.28%'><span style='font-size:167%;color:#E9EB5D'>Distal Chr 7 from ~120 and 132 Mb may modulate</span><span style='font-size:167%;color:#E9EB5D'><i> App</i></span><span style='font-size:200%;color:#E9EB5D;mso-special-format: lastCR;display:none'><i><br> </i></span></div> </div> <img border=0 src="slide0007_image073.png" style='position:absolute;top:93.28%; left:6.62%;width:75.23%;height:3.71%'><img border=0 src="slide0007_image075.png" style='position:absolute;top:15.54%;left:6.62%;width:68.34%;height:75.61%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Physical map of variation in App expression in brain on distal Chr 7 (a blow up of the whole-genome map on the previous slide).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Verdana size=3> You can now see that the X-axis is on a physical scale of megabases (Mb). The QTL peak is roughly between 120 and 132 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. The small irregular colored blocks and marks toward the top of the map mark the locations of genes superimposed on the physical map. Neighboring genes are offset slightly in the vertical axis for display purpose. Note one region of very high gene density from about 120 to 123 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. The orange hash marks along the X-axis represent the number of single nucleotide polymorphisms that distinguish the two parental strains (C57BL/6J and DBA/2J) from each other. We call this the SNP seismograph track (see Glossary for more details). Regions with low numbers of SNPs have closely matched sequences and are less likely to contain QTLs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4. As before, the thin red line shows the additive effect size. By convention the positive values signify the D alleles are associated with higher expression of App in this region of Chr 7 than the B alleles. The maximum effect size is about +0.20 log2 expression units per D allele. The differences been the BB and DD genotypes (BB and DD because each strain has two alleles; one per chromosome) is therefore about 2^0.4 = 1.32 or a 32% increment in DD relative to BB at this locus.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5. If you scroll just under the Physical Map you will see text that reads ÒDISPLAY from XXX Mb TO YYY MbÉ..Ó<span style="mso-spacerun: yes">&nbsp; </span>These physical maps are zoomable, a feature we will exploit to evaluate candidate genes in this QTL interval.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Physical map of variation in App expression in brain on distal Chr 7 (a blow up of the whole-genome map on the previous slide).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=2>1.</font><font face=Verdana size=2> You can now see that the X-axis is on a physical scale of megabases (Mb). The QTL peak is roughly between 120 and 132 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. The small irregular colored blocks and marks toward the top of the map mark the locations of genes superimposed on the physical map. Neighboring genes are offset slightly in the vertical axis for display purpose. Note one region of very high gene density from about 120 to 123 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. The orange hash marks along the X-axis represent the number of single nucleotide polymorphisms that distinguish the two parental strains (C57BL/6J and DBA/2J) from each other. We call this the SNP seismograph track (see Glossary for more details). Regions with low numbers of SNPs have closely matched sequences and are less likely to contain QTLs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>4. As before, the thin red line shows the additive effect size. By convention the positive values signify the D alleles are associated with higher expression of App in this region of Chr 7 than the B alleles. The maximum effect size is about +0.20 log2 expression units per D allele. The differences been the BB and DD genotypes (BB and DD because each strain has two alleles; one per chromosome) is therefore about 2^0.4 = 1.32 or a 32% increment in DD relative to BB at this locus.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>5. If you scroll just under the Physical Map you will see text that reads ÒDISPLAY from XXX Mb TO YYY MbÉ..Ó<span style="mso-spacerun: yes">&nbsp; </span>These physical maps are zoomable, a feature we will exploit to evaluate candidate genes in this QTL interval.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'><img border=0 src="slide0008_image076.png" style='position:absolute;top:34.09%; left:1.72%;width:97.48%;height:61.48%'> <div style='position:absolute;top:-1.06%;left:.79%;width:93.64%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";position:absolute;top:18.36%; left:1.41%;width:97.31%;height:75.51%'><span style='font-family:Verdana; font-size:64%'>Evaluating candidate genes</span><span style='font-family:Verdana; font-size:73%;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </div> <img border=0 src="slide0008_image077.png" style='position:absolute;top:8.83%; left:1.72%;width:34.83%;height:22.61%'> <div style='position:absolute;top:3.35%;left:69.53%;width:33.9%;height:17.84%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:3.96%;left:2.73%;width:93.75%; height:91.08%'> <div style='text-align:center;position:absolute;top:0%;left:0%;width:100.0%; height:33.69%'><span style='font-size:200%;color:#E9EB5D'><i>Right position<br> </i></span></div> <div style='text-align:center;position:absolute;top:32.6%;left:0%;width:100.0%; height:67.39%'><span style='position:absolute;top:0%;left:.41%;width:100.0%'><span style='font-size:200%;color:#E9EB5D'><i>and high </i></span></span><span style='position:absolute;top:50.0%;left:0%;width:100.0%'><span style='font-size:200%;color:#E9EB5D'><i>correlation</i></span></span></div> </div> </div> <div style='position:absolute;top:17.84%;left:75.89%;width:21.72%;height:14.31%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:3.7%;left:0%;width:100.0%;height: 88.88%'> <div style='text-align:center;position:absolute;top:0%;left:6.7%;width:86.58%; height:58.33%'><span style='font-size:267%;color:#E9EB5D'><i><span style="mso-spacerun: yes">&nbsp;</span></i></span><span style='font-size:200%; color:#E9EB5D'><i>= better<br> </i></span></div> <div style='text-align:center;position:absolute;top:56.94%;left:0%;width:100.0%; height:43.05%'><span style='font-size:200%;color:#E9EB5D'><i>candidates</i></span></div> </div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Evaluating candidate genes (CHECKED BOXES) responsible for variability in APP expression:</b></font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>A large number of genes are usually in the QTL interval and are therefore POSITIONAL CANDIDATES, but they will differ greatly in their biological and bioinformatic plausibility. Assume that the QTL has been located between 119 and 131 Mb (12 Mb). There will typically be 12 to 15 genes per Mb, so we might need to evaluate several hundred positional candidates. In this particular case there are about 100 known genes in this interval. Eight of these are highlighted in the table above with check marks in the boxes to the left.<span style="mso-spacerun: yes">&nbsp; </span>We need to highlight and objectively score the biologically relevant subset of all 100 positional candidate genes. We could look through gene ontologies and expression levels to help us shorten the list. An alternate way available using WebQTL is to generate a list of those genes in this interval that have transcripts that co-vary in expression with App expression. That is what the table shows.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. To replicate this table go back to the Trait Data and Analysis Form. Choose to sort correlations by POSITION and select RETURN = 500. Then scroll down the list to Chr 7 and review the subset of positional candidates that share expression with App. You should see a list similar to that shown above. Gtf3c1 is a good biological candidate and has a high covariation in expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>2. Caveat:<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>Of course, the gene or genes that control App expression may not be in this list. A protein coding difference might be the ultimate cause of variation in App transcript level and the expression covariation might be close to zero. Our list may also simply be missing the right transcript since the microarray is not truly comprehensive. Furthermore, even if the list contains the QTL gene, an expression difference may only have been expressed early in development or even in another tissue such as liver. While it is important to recognize these caveats, it is equally important to devise a rational way to rank candidates given existing data. Coexpression is one of several criteria used to evaluate positional candidates. We will see others in the next slide.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>3. We can also assess the likelihood that candidates contain functional polymorphism in promoters and enhancers that affect their expression simply by mapping the transcripts of all candidate genes to see if they Òmap backÓ to the location of gene itself. A transcript that maps to its own location is referred to as a cis QTL. We essentially ask: Which of the transcripts listed in the Correlation Table above (from Gtf3c1 to Zranb1) has variation in expression that maps to Chr 7 at about 120 Mb?<span style="mso-spacerun: yes">&nbsp; </span>The logic of this search is that if a gene controls the level of its own expression it is also much more likely to generate other downstream effects. The Gtf3c1 transcript is a weak cis QTL with a local LRS maximum of about 7.0 (D alleles are high). That is just about sufficient to declare it to be a cis QTL. [No whole genome correction is required and a point-wise p-value of 0.05 is the appropriate test. A p-value of 0.05 is roughly equivalent to an LRS of 6.0 (LOD = 1.3).]</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Evaluating candidate genes (CHECKED BOXES) responsible for variability in APP expression:</b></font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>A large number of genes are usually in the QTL interval and are therefore POSITIONAL CANDIDATES, but they will differ greatly in their biological and bioinformatic plausibility. Assume that the QTL has been located between 119 and 131 Mb (12 Mb). There will typically be 12 to 15 genes per Mb, so we might need to evaluate several hundred positional candidates. In this particular case there are about 100 known genes in this interval. Eight of these are highlighted in the table above with check marks in the boxes to the left.<span style="mso-spacerun: yes">&nbsp; </span>We need to highlight and objectively score the biologically relevant subset of all 100 positional candidate genes. We could look through gene ontologies and expression levels to help us shorten the list. An alternate way available using WebQTL is to generate a list of those genes in this interval that have transcripts that co-vary in expression with App expression. That is what the table shows.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. To replicate this table go back to the Trait Data and Analysis Form. Choose to sort correlations by POSITION and select RETURN = 500. Then scroll down the list to Chr 7 and review the subset of positional candidates that share expression with App. You should see a list similar to that shown above. Gtf3c1 is a good biological candidate and has a high covariation in expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>2. Caveat:<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>Of course, the gene or genes that control App expression may not be in this list. A protein coding difference might be the ultimate cause of variation in App transcript level and the expression covariation might be close to zero. Our list may also simply be missing the right transcript since the microarray is not truly comprehensive. Furthermore, even if the list contains the QTL gene, an expression difference may only have been expressed early in development or even in another tissue such as liver. While it is important to recognize these caveats, it is equally important to devise a rational way to rank candidates given existing data. Coexpression is one of several criteria used to evaluate positional candidates. We will see others in the next slide.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>3. We can also assess the likelihood that candidates contain functional polymorphism in promoters and enhancers that affect their expression simply by mapping the transcripts of all candidate genes to see if they Òmap backÓ to the location of gene itself. A transcript that maps to its own location is referred to as a cis QTL. We essentially ask: Which of the transcripts listed in the Correlation Table above (from Gtf3c1 to Zranb1) has variation in expression that maps to Chr 7 at about 120 Mb?<span style="mso-spacerun: yes">&nbsp; </span>The logic of this search is that if a gene controls the level of its own expression it is also much more likely to generate other downstream effects. The Gtf3c1 transcript is a weak cis QTL with a local LRS maximum of about 7.0 (D alleles are high). That is just about sufficient to declare it to be a cis QTL. [No whole genome correction is required and a point-wise p-value of 0.05 is the appropriate test. A p-value of 0.05 is roughly equivalent to an LRS of 6.0 (LOD = 1.3).]</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-.53%;left:1.72%;width:101.05%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:18.36%;left:.91%;width:97.9%; height:75.51%'><span style='font-family:Verdana;font-size:64%'>Physical maps are zoomable</span><span style='font-family:Verdana;font-size:73%;mso-special-format: lastCR;display:none'><i><br> </i></span></div> </div> <img border=0 src="slide0009_image078.png" style='position:absolute;top:8.83%; left:3.31%;width:89.93%;height:41.69%'><img border=0 src="slide0009_image079.png" style='position:absolute;top:44.52%;left:7.54%; width:42.91%;height:48.76%'><img border=0 src="slide0009_image080.png" style='position:absolute;top:94.52%;left:0%;width:97.08%;height:3.71%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>An even higher blow-up of part of the Chr 7 physical map of variation in App expression in brain.<span style="mso-spacerun: yes">&nbsp; </span>The QTL region actually extends from about 119 to 129.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. As mentioned in the previous slide another important approach to ranking candidates is based on the number of sequence variants that distinguish the parental strains. If we were sure that the sequences of the gene, its promoter, and its enhancers were identical between the strains then we could discount--but not eliminate--that gene as a candidate. The Gtf3c1 candidate almost falls into this category: of 663 known SNPs in and around this gene, only four differ between C57BL/6J and DBA/2J. Gtf3c1 is essentially identical-by-descent in these strains and is a less likely candidate. In contrast, if the two alleles of the gene have dozens of functional variants in exons, promoters, enhancers, and splice sites, then it becomes a higher priority candidate.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Of course it only takes a single critical sequence variant to generate downstream effects. The argument above is really about the prior probabilities. Where would you place your bets given the information at hand?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2.<span style="mso-spacerun: yes">&nbsp; </span>If you scroll down the INTERVAL ANALYST you will find that Ctbp2 is a particularly interesting candidate that contains lots of SNPs (n = 75 and a SNP density of 0.55 SNP/Kb). Ctbp2 is also closer to our QTL peak than was Gtf3c1. Not only does Ctbp2 contain lots of SNPs but it is also is associated with a powerful cis QTL with an LRS of 24.2 (divide by 4.61 to get the equivalent LOD score of 5.25).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3.<span style="mso-spacerun: yes">&nbsp; </span>At this high magnification, individual genes are distinct. They are color coded by their density of SNPs. Bright orange represents those genes that have a high SNP density (C57BL/6J versus DBA/2J), black represents genes with low SNP density. Roll the cursor over a gene block and its name will pop up, along with information on exon number.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4.<span style="mso-spacerun: yes">&nbsp; </span>Beneath the physical map you will find an INTERVAL ANALYST table that lists information on known genes in the region on which you have zoomed the Physical Map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5.<span style="mso-spacerun: yes">&nbsp; </span>As always: error-checking is important. Some genes may be missing from the Interval Analyst (recent additions or errors of omission). In this case the Zranb1 gene that is located just proximal to Ctbp2 is not listed in the INTERVAL ANALYST. Double-check the interval using the Genome Browser links (blue and beige horizontal bars) at the top of the PHYSICAL MAP.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>An even higher blow-up of part of the Chr 7 physical map of variation in App expression in brain.<span style="mso-spacerun: yes">&nbsp; </span>The QTL region actually extends from about 119 to 129.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. As mentioned in the previous slide another important approach to ranking candidates is based on the number of sequence variants that distinguish the parental strains. If we were sure that the sequences of the gene, its promoter, and its enhancers were identical between the strains then we could discount--but not eliminate--that gene as a candidate. The Gtf3c1 candidate almost falls into this category: of 663 known SNPs in and around this gene, only four differ between C57BL/6J and DBA/2J. Gtf3c1 is essentially identical-by-descent in these strains and is a less likely candidate. In contrast, if the two alleles of the gene have dozens of functional variants in exons, promoters, enhancers, and splice sites, then it becomes a higher priority candidate.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Of course it only takes a single critical sequence variant to generate downstream effects. The argument above is really about the prior probabilities. Where would you place your bets given the information at hand?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2.<span style="mso-spacerun: yes">&nbsp; </span>If you scroll down the INTERVAL ANALYST you will find that Ctbp2 is a particularly interesting candidate that contains lots of SNPs (n = 75 and a SNP density of 0.55 SNP/Kb). Ctbp2 is also closer to our QTL peak than was Gtf3c1. Not only does Ctbp2 contain lots of SNPs but it is also is associated with a powerful cis QTL with an LRS of 24.2 (divide by 4.61 to get the equivalent LOD score of 5.25).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3.<span style="mso-spacerun: yes">&nbsp; </span>At this high magnification, individual genes are distinct. They are color coded by their density of SNPs. Bright orange represents those genes that have a high SNP density (C57BL/6J versus DBA/2J), black represents genes with low SNP density. Roll the cursor over a gene block and its name will pop up, along with information on exon number.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>4.<span style="mso-spacerun: yes">&nbsp; </span>Beneath the physical map you will find an INTERVAL ANALYST table that lists information on known genes in the region on which you have zoomed the Physical Map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>5.<span style="mso-spacerun: yes">&nbsp; </span>As always: error-checking is important. Some genes may be missing from the Interval Analyst (recent additions or errors of omission). In this case the Zranb1 gene that is located just proximal to Ctbp2 is not listed in the INTERVAL ANALYST. Double-check the interval using the Genome Browser links (blue and beige horizontal bars) at the top of the PHYSICAL MAP.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:0%;left:1.72%;width:106.35%;height:7.24%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:12.19%;left:.87%;width:99.12%; height:90.24%'><span style='font-family:Verdana;font-size:64%'>Evaluating </span><span style='font-family:Verdana;font-size:64%'><i>Ctbp2</i></span><span style='font-family:Verdana;font-size:64%'> as a candidate QTL for </span><span style='font-family:Verdana;font-size:64%'><i>App</i></span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </div> <img border=0 src="slide0010_image082.png" style='position:absolute;top:94.52%; left:3.31%;width:96.68%;height:4.24%'><img border=0 src="slide0010_image083.png" style='position:absolute;top:7.77%;left:3.31%;width:48.34%;height:40.28%'> <div class=O style='position:absolute;top:52.82%;left:55.23%;width:35.76%; height:37.98%'><span style='position:absolute;top:0%;left:0%;width:94.81%'><span style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%; color:#E9EB5D'><i> the </i></span></span><span style='position:absolute; top:16.74%;left:0%;width:95.92%'><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2 </i></span><span style='font-size:233%;color:#E9EB5D'>cis QTL, </span></span><span style='position:absolute;top:33.48%;left:0%;width:98.14%'><span style='font-size:233%;color:#E9EB5D'>but is detected </span></span><span style='position:absolute;top:49.76%;left:0%;width:94.81%'><span style='font-size:233%;color:#E9EB5D'>only in the </span></span><span style='position:absolute;top:66.51%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'>Rosen striatum </span></span><span style='position:absolute;top:83.25%;left:0%;width:94.81%'><span style='font-size:233%;color:#E9EB5D'>data set.</span><span style='font-size: 233%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> <div class=O style='position:absolute;top:16.25%;left:56.02%;width:48.21%; height:12.72%'><span style='position:absolute;top:0%;left:0%;width:94.78%'><span style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%; color:#E9EB5D'><i> the App QTL </i></span></span><span style='position:absolute; top:48.61%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'>in the INIA data set. </span><span style='font-size:233%;color:#E9EB5D;mso-special-format: lastCR;display:none'><i><br> </i></span></span></div> <img border=0 src="slide0010_image084.png" style='position:absolute;top:39.39%; left:3.31%;width:48.47%;height:53.35%'><img border=0 src="slide0010_image085.png" style='position:absolute;top:10.95%;left:35.36%; width:.39%;height:72.79%'><img border=0 src="slide0010_image086.png" style='position:absolute;top:10.95%;left:31.52%;width:.39%;height:72.79%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This slide illustrates one reason why Ctbp2 should be considered a high priority positional candidate gene that may modulate the expression level of App.<span style="mso-spacerun: yes">&nbsp; </span>Ctbp2 is a strong cis QTL in some brain regions (here the data are taken from the striatum).<span style="mso-spacerun: yes">&nbsp; </span>If Ctbp2 contains variants that modulate its own expression then these expression differences may produce many downstream effects. Of course, we now want to know much more about the known biology of Ctbp2. What kind of gene is it? To begin to answer that question we can use a number of resources listed in the LINKS page.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Verdana size=3> The App QTL is bimodal. Perhaps there are actually two causal factors in this region--one close to 123 Mb and the other close to 127 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. The precision of QTL mapping depends on several factors, including the effect size and interactions among QTLs modulating a trait, the number of genetic individuals that are studied, and the distribution of recombinations in the study population.<span style="mso-spacerun: yes">&nbsp; </span>In the case above, the QTL(s) are likely to be confined to the interval from 120 to 132 Mb. The bootstrap test (yellow bars shown in some of the previous slides) can be usual for estimating the consistency of QTL peaks.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>This slide illustrates one reason why Ctbp2 should be considered a high priority positional candidate gene that may modulate the expression level of App.<span style="mso-spacerun: yes">&nbsp; </span>Ctbp2 is a strong cis QTL in some brain regions (here the data are taken from the striatum).<span style="mso-spacerun: yes">&nbsp; </span>If Ctbp2 contains variants that modulate its own expression then these expression differences may produce many downstream effects. Of course, we now want to know much more about the known biology of Ctbp2. What kind of gene is it? To begin to answer that question we can use a number of resources listed in the LINKS page.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=2>1.</font><font face=Verdana size=2> The App QTL is bimodal. Perhaps there are actually two causal factors in this region--one close to 123 Mb and the other close to 127 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. The precision of QTL mapping depends on several factors, including the effect size and interactions among QTLs modulating a trait, the number of genetic individuals that are studied, and the distribution of recombinations in the study population.<span style="mso-spacerun: yes">&nbsp; </span>In the case above, the QTL(s) are likely to be confined to the interval from 120 to 132 Mb. The bootstrap test (yellow bars shown in some of the previous slides) can be usual for estimating the consistency of QTL peaks.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+LoadSld( gId );
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:0%;left:1.72%;width:101.05%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:18.36%;left:.91%;width:97.9%; height:75.51%'><span style='font-family:Verdana;font-size:64%'>Evaluating </span><span style='font-family:Verdana;font-size:64%'><i>Ctbp2</i></span><span style='font-family:Verdana;font-size:64%'> using other resources</span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </div> <img border=0 src="slide0011_image087.png" style='position:absolute;top:10.07%; left:1.72%;width:48.21%;height:53.35%'><img border=0 src="slide0011_image088.png" style='position:absolute;top:40.81%;left:19.2%; width:78.54%;height:54.77%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Ctbp2 should also be considered a high priority biological candidate gene responsible for modulating App expression levels. The C-terminal binding protein 2 is a transcriptional co-repressor also known as Ribeye.</font><font face=Times-Roman size=3> The gene produces two transcripts encoding distinct proteins. The short form is a transcriptional repressor that binds a Pro-X-Asp-Leu-Ser peptide motif and interacts with several transcription factors including EVI1, ZFPM1, and ZFHX1A (aka TCF8, deltaEF1). The longer isoform is a major component of specialized synapses in photoreceptors. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. To find out more about CTBP2 protein and the Ctbp2 gene, link to iHOP at http://www.pdg.cnb.uam.es/UniPub/iHOP/ and type in CTBP2</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Try Arrowsmith at http://arrowsmith.psych.uic.edu/cgi-test/arrowsmith_uic/pubsmith.cgi</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. Both APP and CTBP2 are involved in oxidoreducatase activity or Notch signaling. To establish this common gene ontology visit NCBI<span style="mso-spacerun: yes">&nbsp; </span>http://www.ncbi.nih.gov/entrez/query.fcgi?db=gene and enter each gene symbol.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. You can get interesting hints regarding Ctbp2 expression partners by examining the genetic correlations between Ctbp2 probe set 1422887_a_at and all other transcripts on the M430 Affymetrix array. Use the Striatum data set because we already know from previous work (the previous slide) that this gene is a cis QTL.<span style="mso-spacerun: yes">&nbsp; </span>You should be able to show that Ctbp2 and Notch3 have antagonistic expression patterns in striatum. The negative genetic correlation with E2f4 is even stronger. The transcript also has a high positive genetic correlation with Rdh14. Of particular interest with respect to APP protein processing, Ctbp2 covaries positively with Bace2 (the transcript of the beta site APP-cleaving enzyme 2).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Ctbp2 should also be considered a high priority biological candidate gene responsible for modulating App expression levels. The C-terminal binding protein 2 is a transcriptional co-repressor also known as Ribeye.</font><font face=Times-Roman size=2> The gene produces two transcripts encoding distinct proteins. The short form is a transcriptional repressor that binds a Pro-X-Asp-Leu-Ser peptide motif and interacts with several transcription factors including EVI1, ZFPM1, and ZFHX1A (aka TCF8, deltaEF1). The longer isoform is a major component of specialized synapses in photoreceptors. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>1. To find out more about CTBP2 protein and the Ctbp2 gene, link to iHOP at http://www.pdg.cnb.uam.es/UniPub/iHOP/ and type in CTBP2</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Try Arrowsmith at http://arrowsmith.psych.uic.edu/cgi-test/arrowsmith_uic/pubsmith.cgi</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. Both APP and CTBP2 are involved in oxidoreducatase activity or Notch signaling. To establish this common gene ontology visit NCBI<span style="mso-spacerun: yes">&nbsp; </span>http://www.ncbi.nih.gov/entrez/query.fcgi?db=gene and enter each gene symbol.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. You can get interesting hints regarding Ctbp2 expression partners by examining the genetic correlations between Ctbp2 probe set 1422887_a_at and all other transcripts on the M430 Affymetrix array. Use the Striatum data set because we already know from previous work (the previous slide) that this gene is a cis QTL.<span style="mso-spacerun: yes">&nbsp; </span>You should be able to show that Ctbp2 and Notch3 have antagonistic expression patterns in striatum. The negative genetic correlation with E2f4 is even stronger. The transcript also has a high positive genetic correlation with Rdh14. Of particular interest with respect to APP protein processing, Ctbp2 covaries positively with Bace2 (the transcript of the beta site APP-cleaving enzyme 2).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-.35%;left:3.31%;width:93.64%;height:14.48%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:"-220 0 -216";mso-margin-left-alt:292; mso-text-indent-alt:0;position:absolute;top:0%;left:1.13%;width:97.59%; height:51.21%'><span style='mso-special-format:nobullet;display:none; color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'>Summary of Part 2</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR; display:none'><br> </span></div> </div> <div style='position:absolute;top:15.01%;left:5.96%;width:101.19%;height:31.09%'> <div class=O1 style='mso-margin-left-alt:432'></div> <div class=O2 style='mso-margin-left-alt:720'></div> <div class=O3 style='mso-margin-left-alt:1008'></div> <div class=O4 style='mso-margin-left-alt:1296'></div> <div class=O style='mso-margin-left-alt:144;position:absolute;top:0%; left:0%;width:86.25%;height:14.2%'><span style='position:absolute;top:0%; left:2.88%;width:97.11%'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:"numbullet3\,1"; position:absolute;left:-2.96%;font-family:Arial'>1.</span></span><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>Covered the basics of QTL analysis and mapping.<br> </span></span></div> <div class=O style='mso-margin-left-alt:144;position:absolute;top:14.2%; left:0%;width:86.25%;height:14.2%'><span style='position:absolute;top:0%; left:2.88%;width:97.11%'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:"numbullet3\,1"; position:absolute;left:-2.96%;font-family:Arial'>2.</span></span><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>Reviewed difference between genetic and physical maps.<br> </span></span></div> <div class=O style='mso-margin-left-alt:144;position:absolute;top:28.4%; left:0%;width:100.0%;height:42.61%'><span style='position:absolute;top:0%; left:2.48%;width:97.51%'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:"numbullet3\,1"; position:absolute;left:-2.55%;font-family:Arial'>3.</span></span><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>Discussed interpreting features of QTL maps including the LRS function, </span></span><span style='position: absolute;top:33.33%;left:2.48%;width:91.23%'><span style='font-family:"Gill Sans"; font-size:167%;color:#E9EB5D;mso-color-index:3'>the additive effect function, the bootstrap bars, and the permutation </span></span><span style='position: absolute;top:68.0%;left:2.48%;width:83.76%'><span style='font-family:"Gill Sans"; font-size:167%;color:#E9EB5D;mso-color-index:3'>thresholds.<br> </span></span></div> <div class=O style='mso-margin-left-alt:144;position:absolute;top:71.59%; left:0%;width:88.35%;height:14.2%'><span style='position:absolute;top:0%; left:2.81%;width:97.18%'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:"numbullet3\,1"; position:absolute;left:-2.89%;font-family:Arial'>4.</span></span><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>Illustrated techniques to generate a list of positional candidates.<br> </span></span></div> <div class=O style='mso-margin-left-alt:144;position:absolute;top:85.79%; left:0%;width:86.25%;height:14.2%'><span style='position:absolute;top:0%; left:2.88%;width:97.11%'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:"numbullet3\,1"; position:absolute;left:-2.96%;font-family:Arial'>5.</span></span><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>Discussed some factors used to evaluate candidate genes.</span></span></div> </div> <div class=O style='position:absolute;top:58.65%;left:5.96%;width:106.22%; height:22.26%'><span style='position:absolute;top:0%;left:0%;width:84.53%'><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'>What does a QTL signify? A good QTL is a claim that a particular </span></span><span style='position:absolute;top:19.84%;left:0%;width:96.38%'><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'>chromosomal region contains a causal source of variation in the phenotype. </span></span><span style='position:absolute;top:39.68%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'>The importance of this hypothesis depends on the quality and relevance of the </span></span><span style='position:absolute;top:59.52%;left:0%;width:90.89%'><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'>phenotype and the statistical strength of the QTL. As usual, test and be </span></span><span style='position:absolute;top:79.36%;left:0%;width:84.53%'><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'>skeptical.</span></span></div> </div> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+}
+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>By clicking on the CORRELATION of the Atcay transcript to the App transcript, you can generate a Correlation plot between these two transcripts. In this App and Atcay scatterplot, each point is a strain mean value. For example, BXD33 and BXD8 have low App and Atcay expressions. The two parental strains and the F1 are also included in this plot.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content="Aug-15-05: Test Questions"> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
+gId="slide0013.htm"
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+</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:0%;left:1.72%;width:101.05%;height:8.65%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:18.36%;left:.91%;width:97.9%; height:75.51%'><span style='font-family:Verdana;font-size:64%'>Test Questions</span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </div> <div class=O style='position:absolute;top:11.83%;left:4.37%;width:108.34%; height:6.53%'><span style='font-size:233%;color:#E9EB5D'>1. Evaluate candidates for the Chr 3 </span><span style='font-size:233%;color:#E9EB5D'><i>App</i></span><span style='font-size:233%;color:#E9EB5D'> QTL.</span><span style='font-size:233%; color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></div> <div class=O style='position:absolute;top:24.02%;left:4.9%;width:100.92%; height:12.72%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'>2. Do </span><span style='font-size:233%; color:#E9EB5D'><i>App</i></span><span style='font-size:233%;color:#E9EB5D'> and </span><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2</i></span><span style='font-size:233%;color:#E9EB5D'> expression share any </span></span><span style='position:absolute;top:50.0%;left:0%;width:93.43%'><span style='font-size:233%;color:#E9EB5D'>other QTLs beside that on Chr 7?</span><span style='font-size:233%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> <div class=O style='position:absolute;top:43.99%;left:4.9%;width:102.91%; height:19.08%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'>3. Can you exploit literature mining tools to </span></span><span style='position:absolute;top:33.33%;left:0%;width:98.45%'><span style='font-size:233%;color:#E9EB5D'>find a strong relationship between </span><span style='font-size:233%;color:#E9EB5D'><i>App</i></span><span style='font-size: 233%;color:#E9EB5D'> and </span></span><span style='position:absolute; top:65.74%;left:0%;width:91.63%'><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2</i></span><span style='font-size:233%;color:#E9EB5D'>? </span><span style='font-size:233%; color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> <div class=O style='position:absolute;top:69.08%;left:4.9%;width:110.19%; height:12.72%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'>4. Why might the cis QTL for </span><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2</i></span><span style='font-size: 233%;color:#E9EB5D'> expression </span></span><span style='position:absolute; top:50.0%;left:0%;width:89.66%'><span style='font-size:233%;color:#E9EB5D'>only be detected in the striatum data set?</span><span style='font-size:233%; color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>A group of traits from many different databases can be selected and brought together for joint analysis. In this case all of the content of the BXD SELECTIONS is from a single BRAIN database, the top 20 neighbors of the App transcript from the Correlation Results table. Eight of these neighbors plus App is shown in the slide.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=2>1.</font><font face=Helvetica size=2>All of items in the BXD SELECTIONS were selected using the SELECT ALL button</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>2. The buttons at the top (and bottom) of this page can do some cool stuff. We will work with NETWORK GRAPH first.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>3. Think of the SELECTIONS as your shopping cart. You go to different aisles in the supermarket to acquire different types of items of interest. These could include transcripts, classical phenotypes (longevity, brain weight, prepulse inhibition, iron levels in midbrain). ÒChecking outÓ in this case involves doing some analysis with the items in the cart.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>4. Different tools handle different numbers of items. Most will handle up to 100 traits.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-277 0 -1";mso-margin-left-alt:232; mso-text-indent-alt:0;mso-line-spacing:"-277 0 -1";mso-margin-left-alt:232; mso-text-indent-alt:0'><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:73%'>The GeneNetwork and WebQTL : PART 2<span style="mso-spacerun: yes">&nbsp;&nbsp; </span><br> </span></span></layer><script>
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0015_image034.png b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0015_image034.png
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0015_notes_pane.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0015_notes_pane.htm
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Going back to the Trait Data and Analysis Form window, we have computed the correlations between strain variation in App expression level and other classical phenotypes that have already been measured in many of the same BXD strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=2>1.</font><font face=Helvetica size=2>The number of common strains varies widely--in this case from 14 to 23 strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>2. We can add these traits (four are selected) to our BXD SELECTIONS window.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0016.htm
@@ -0,0 +1,919 @@
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+wd = 0.09 * g_width;
+document.write(
+'<img border=0 src="slide0002_image017.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.71 * g_height; myleft = 0.58 * g_width; myheight = 0.26 * g_height; mywidth = 0.2 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.03 * g_height; myleft = 0.01 * g_width; myheight = 0.22 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.15 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>20 generations </b></span></span></layer><script>
+ mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>brother-sister </b></span></span></layer><script>
+ mytop = 0.12 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>matings</b></span><span style='font-family:Arial;font-size: 233%;color:#FFFF99;display:none'><b><br> </b></span></span></layer></div> </layer><script>
+ mytop = 0.16 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:233%; color:#FFFF99;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.6 * g_height; myleft = 0.5 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.5 * g_width;
+yy = 0.6 * g_height;
+ht = 0.03 * g_height;
+wd = 0.09 * g_width;
+document.write(
+'<img border=0 src="slide0002_image018.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.62 * g_height; myleft = 0.54 * g_width; myheight = 0.28 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.54 * g_width;
+yy = 0.62 * g_height;
+ht = 0.28 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image019.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.61 * g_height; myleft = 0.79 * g_width; myheight = 0.03 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.79 * g_width;
+yy = 0.61 * g_height;
+ht = 0.03 * g_height;
+wd = 0.1 * g_width;
+document.write(
+'<img border=0 src="slide0002_image020.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.62 * g_height; myleft = 0.83 * g_width; myheight = 0.28 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.83 * g_width;
+yy = 0.62 * g_height;
+ht = 0.28 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image021.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.92 * g_height; myleft = 0.21 * g_width; myheight = 0.09 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD1</span><span style='font-family:Arial;font-size:150%;color:#484848;display:none'><br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#484848;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.92 * g_height; myleft = 0.51 * g_width; myheight = 0.09 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD2<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.92 * g_height; myleft = 0.8 * g_width; myheight = 0.05 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:"-173 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;mso-line-spacing:"-173 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>BXD80</span></div> </layer></div> </layer><script>
+ mytop = 0.9 * g_height; myleft = 0.64 * g_width; myheight = 0.17 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.15 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-341 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:300%;color:#FFFF99'>+ É +<br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-341 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:300%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.62 * g_height; myleft = 0.04 * g_width; myheight = 0.12 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F2<br> </b></span></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format: lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.18 * g_width; myheight = 0.01 * g_height; mywidth = 0.75 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.18 * g_width;
+yy = 0.54 * g_height;
+ht = 0.01 * g_height;
+wd = 0.75 * g_width;
+document.write(
+'<img border=0 src="slide0002_image022.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.17 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.17 * g_width;
+yy = 0.54 * g_height;
+ht = 0.03 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0002_image023.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.32 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.32 * g_width;
+yy = 0.54 * g_height;
+ht = 0.03 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0002_image024.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.48 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.48 * g_width;
+yy = 0.54 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0002_image025.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.62 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.62 * g_width;
+yy = 0.54 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0002_image026.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.77 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.77 * g_width;
+yy = 0.54 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0002_image027.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.92 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.92 * g_width;
+yy = 0.54 * g_height;
+ht = 0.03 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0002_image028.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.44 * g_height; myleft = 0.49 * g_width; myheight = 0.1 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.49 * g_width;
+yy = 0.44 * g_height;
+ht = 0.1 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0002_image029.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.15 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.15 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image030.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.3 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.3 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image031.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.46 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.46 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image032.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.6 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.6 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image033.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.75 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.75 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image034.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.9 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.9 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0002_image035.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.84 * g_height; myleft = 0.56 * g_width; myheight = 0.01 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.56 * g_width;
+yy = 0.84 * g_height;
+ht = 0.01 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0002_image036.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.8 * g_height; myleft = 0.25 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.25 * g_width;
+yy = 0.8 * g_height;
+ht = 0.11 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0002_image037.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.84 * g_height; myleft = 0.86 * g_width; myheight = 0.02 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.86 * g_width;
+yy = 0.84 * g_height;
+ht = 0.02 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0002_image038.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.75 * g_height; myleft = 0.01 * g_width; myheight = 0.13 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'><b>BXD RI<br> </b></span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'><b>Strain set<br> </b></span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.12 * g_height; myleft = 0.14 * g_width; myheight = 0.06 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.14 * g_width;
+yy = 0.12 * g_height;
+ht = 0.06 * g_height;
+wd = 0.1 * g_width;
+document.write(
+'<img border=0 src="slide0002_image039.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.76 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.76 * g_width;
+yy = 0.1 * g_height;
+ht = 0.08 * g_height;
+wd = 0.14 * g_width;
+document.write(
+'<img border=0 src="slide0002_image040.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.2 * g_height; myleft = 0.17 * g_width; myheight = 0.13 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>fully<br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>inbred<br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.38 * g_height; myleft = 0.02 * g_width; myheight = 0.09 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>isogenic<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.01 * g_width; myheight = 0.13 * g_height; mywidth = 0.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>hetero-<br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>geneous<br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.74 * g_height; myleft = 0.29 * g_width; myheight = 0.21 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.19 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.15 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>Recombined </span></span></layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>chromosomes </span></span></layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>are needed for </span></span></layer><script>
+ mytop = 0.12 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>mapping<br> </span></span></layer></div> </layer><script>
+ mytop = 0.16 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.25 * g_width; myheight = 0.09 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>female<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.64 * g_width; myheight = 0.09 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>male<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.3 * g_height; myleft = 0.78 * g_width; myheight = 0.09 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:117%;color:#FFFF99'>chromosome pair</span><span style='font-family:Arial;font-size:150%;color:#FFFF99; display:none'><br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.15 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.31 * g_height; myleft = 0.65 * g_width; myheight = 0.02 * g_height; mywidth = 0.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.65 * g_width;
+yy = 0.31 * g_height;
+ht = 0.02 * g_height;
+wd = 0.12 * g_width;
+document.write(
+'<img border=0 src="slide0002_image041.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.86 * g_height; myleft = 0.03 * g_width; myheight = 0.14 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.12 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:#E9EB5D'>Inbred<br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The next few slides provide a short introduction to mapping the loci that are responsible for variation in a trait such as App expression level. These modulatory regions of the genome are sometimes called quantitative trait loci or QTLs. You may want to do some independent reading on this topic if this is your first exposure to QTL analysis.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The genetic reference population (GRP) of BXD recombinant inbred strains were originally generated about 25 years ago by Benjamin Taylor at The Jackson Laboratory. He crossed female C57BL/6J mice with male DBA/2J mice to generate the F1 and F2 progeny. At the bottom of this slide we have schematized one chromosome pair from three of the BXD RI strains.<span style="mso-spacerun: yes">&nbsp; </span>The dashed vertical lines that lead to the final BXD RI lines involve 21 full sib matings (about 7 years of breeding). Some lines die out during inbreeding. For example, there is no longer any BXD3 strain.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. Over the last decade, our group (Lu Lu and Rob Williams) and Jeremy Peirce and Lee Silver at Princeton have enlarged Ben TaylorÕs set. There are now just over 80 BXD strains. They have all been genotyped using about 13,700 markers (SNPs and microsatellites). These markers are used to define the ÒblueÓ and ÒredÓ regions of the chromosomes as shown in the figure above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. Chromosomes of RI GRPs usually have about 4 times as many recombinations as those of F2 animals. However, unlike an F2, both chromosomes of an RI are identical. Therefore, 50 RI strains contain as many recombinations as 100 F2 animals.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. BXD43 through BXD100 were generated using a special method that resulted in a further doubling of the average recombination density per chromosome. The entire set of 80 BXDs therefore contains as many recombinations as about 260 F2 animals.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0016_image036.png b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0016_image036.png
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0016_notes_pane.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0016_notes_pane.htm
new file mode 100755
index 00000000..f5776dad
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0016_notes_pane.htm
@@ -0,0 +1,5 @@
+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>We have computed the Network Graph, now using other types of traits.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Saline Hot Plate Latency is the green node labeled 10020.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Freezing (fear) is the green node labeled 10447.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Notes:</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.24 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.24 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:267%;color:#E2EBF0'>D2 strain</span><span style='font-family:Palatino;font-size:333%;color:#FBFDB8;display:none'><br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:333%;color:#FBFDB8;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.43 * g_height; myleft = 0.71 * g_width; myheight = 0.19 * g_height; mywidth = 0.25 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.02 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.24 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.24 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:267%;color:#F6BF69'>B6 strain</span><span style='font-family:Palatino;font-size:333%;color:#F6BF69;display:none'><br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:333%;color:#F6BF69;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.17 * g_height; myleft = 0.31 * g_width; myheight = 0.42 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.31 * g_width;
+yy = 0.17 * g_height;
+ht = 0.42 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0003_image056.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.4 * g_height; myleft = 0.31 * g_width; myheight = 0.03 * g_height; mywidth = 0.39 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.31 * g_width;
+yy = 0.4 * g_height;
+ht = 0.03 * g_height;
+wd = 0.39 * g_width;
+document.write(
+'<img border=0 src="slide0003_image057.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.2 * g_height; myleft = 0.38 * g_width; myheight = 0.26 * g_height; mywidth = 0.3 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.23 * g_height; mywidth = 0.27 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.13 * g_height; mywidth = 0.27 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-320 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.27 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'><i>amount of </i></span></span></layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.27 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'><i>transcript</i></span><span style='font-family:Palatino;font-size:367%;color:#E9EB5D;display:none'><br> </span></span></layer></div> </layer><script>
+ mytop = 0.14 * g_height; myleft = 0 * g_width; myheight = 0.09 * g_height; mywidth = 0.27 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-422 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:367%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.06 * g_height; myleft = 0.72 * g_width; myheight = 0.19 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:267%;color:white'>4 </span><span style='font-size: 233%;color:white'>units</span><span style='font-family:Palatino;font-size:333%; color:#E9EB5D;display:none'><br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.29 * g_height; myleft = 0.72 * g_width; myheight = 0.19 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.02 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:267%;color:white'>2</span><span style='font-size: 233%;color:white'> units</span><span style='font-family:Palatino;font-size: 333%;color:#E9EB5D;display:none'><br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.15 * g_height; myleft = 0.31 * g_width; myheight = 0.05 * g_height; mywidth = 0.4 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.31 * g_width;
+yy = 0.15 * g_height;
+ht = 0.05 * g_height;
+wd = 0.4 * g_width;
+document.write(
+'<img border=0 src="slide0003_image058.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.17 * g_height; myleft = 0.09 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.17 * g_height; myleft = 0.17 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.79 * g_height; myleft = 0.05 * g_width; myheight = 0.13 * g_height; mywidth = 1.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.12 * g_height; mywidth = 1.02 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:"-256 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;mso-line-spacing:"-256 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.02 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E2EBF0'>D</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%; color:#E9EB5D'>and </span><span style='font-size:233%;color:#F6BF69'>B</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%; color:#E9EB5D'>may be SNP-like variants in the promoter </span></span></layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 1.02 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:200%;color:#E9EB5D'>itself (cis QTL) or in </span><span style='font-size:200%;color:#EAEC5E'>upstream genes (trans QTLs)</span><span style='font-size:300%;color:#E9EB5D'>.</span></span></layer></div> </layer></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0.04 * g_width; myheight = 0.16 * g_height; mywidth = 0.22 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.22 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D'>UPSTREAM<br> </span></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.22 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D'>modulators<br> </span></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.01 * g_width; myheight = 0.05 * g_height; mywidth = 0.2 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D; mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0.44 * g_width; myheight = 0.17 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:300%;color:#E2EBF0'>High<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.63 * g_height; myleft = 0.08 * g_width; myheight = 0.17 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.63 * g_height; myleft = 0.15 * g_width; myheight = 0.17 * g_height; mywidth = 0.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+//-->
+</script></div> </layer><script>
+ mytop = 0.27 * g_height; myleft = 0.05 * g_width; myheight = 0.12 * g_height; mywidth = 0.2 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+</script> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:200%;color:#E9EB5D'><i>trans QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>This slide is illustrates two major types of QTLs that modulate variability in transcript-relative steady state abundance.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>1. cis QTLs are defined as QTLs that are closely linked to the gene whose transcript is the measured trait. For example, a polymorphism in the promoter that affects binding of a transcription factor. However, cis QTLs can be far upstream or downstream polymorphisms in enhancers or may be in 3Õ UTR binding sites that affect message stability.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. trans QTLs map far enough away from the location of the gene that gives rise to the transcript that is being measured so that we can be fairly certain that the QTL is not in the gene itself. The most blatant type of trans QTL would be a polymorphism in a transcription factor. But in the majority of cases, the trans QTLs can be far removed in a mechanistic sense from the actual events modulating transcript abundance. That is why there are three overlapping arrows in the figure.<span style="mso-spacerun: yes">&nbsp; </span>The way in which an upstream polymorphism influences a downstream difference in mRNA abundance can be indirect. Effects can:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>a.<span style="mso-spacerun: yes">&nbsp; </span>cross tissue types (a polymorphic liver enzyme may affect CNS gene expression)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>b.<span style="mso-spacerun: yes">&nbsp; </span>cross time (the modulator is only expressed for one day during development but has permanent effects in adults)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>c.<span style="mso-spacerun: yes">&nbsp; </span>may be contingent on environmental factors (heat shock may trigger the expression of a polymorphic factor that affects mRNA abundance).</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Please bring the Trait Data and Analysis window to the front and look for the Interval Mapping button. Confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Mapping Scale is set to GENETIC</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Permutation test YES<span style="mso-spacerun: yes">&nbsp; </span>(2000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Bootstrap test YES (2000 is the default number)</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Part 2: Discovering upstream modulators and quantitative trait loci (QTLs). A quantitative trait locus is a chromosomal region that harbors one or a few polymorphic gene loci that influence a trait. We are going to be looking for QTLs that modulate the steady state expression level of App in the adult mouse forebrain.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'><i>WebQTL searches for upstream controllers</i></span><span style='font-family:Verdana; font-size:73%;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This is a major output type: a so-called full-genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromosomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans at almost 0 megabases). The blue labels along the bottom of the figure list a subset of the 3795 markers that were used in mapping.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The thick blue wavy line running across chromosomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of all markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The red line and the red axis to the far right provide an estimate of the effect that a QTL has on expression of App (this estimate of the so-called additive effect tends to be too high). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher trait values. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and those that have the D/D genotype at a specific marker. For example, on distal Chr 7 the red line peaks at a value of about 0.2. That means that this region of chromosome 2 is responsible for a 0.4 unit expression difference between B/B strains and the D/D strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides a method to evaluate whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But using the bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 3 and Chr 7 under the LRS peaks. That is somewhat reassuring. But notice that a substantial number of bootstrap are scattered around on other chromosomes. About 30% of the bootstrap resamples have a peak on Chr 7. That is pretty good, but does makes us realize that the sample we are working with is still quite small and fragile.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The horizontal dashed lines at 10.5 and 17.3 are the likelihood ratio statistic (LRS) values associated with the suggestive and significant genome-wide probabilities that were established by permutations of phenotypes across genotypes. We shuffle randomly 2000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 17.3. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 3 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations. Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. If you click on the body of the map, say on the blue line, then you will generate a view on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. If you click on a marker symbol, then you will generate a new Trait data and Analysis window with the genotypes loaded into the window just like any other trait. More on this in Section 3.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4. You can drag these maps off of the browser window and onto your desktop. They will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5. There is also an option at the bottom of the page to download a 2X higher resolution image of this plot for papers and presentations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>6. You can also download the results of the analysis in a text format</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The next few slides provide a short introduction to mapping the loci that are responsible for variation in a trait such as App expression level. These modulatory regions of the genome are sometimes called quantitative trait loci or QTLs. You may want to do some independent reading on this topic if this is your first exposure to QTL analysis.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The genetic reference population (GRP) of BXD recombinant inbred strains were originally generated about 25 years ago by Benjamin Taylor at The Jackson Laboratory. He crossed female C57BL/6J mice with male DBA/2J mice to generate the F1 and F2 progeny. At the bottom of this slide we have schematized one chromosome pair from three of the BXD RI strains.<span style="mso-spacerun: yes">&nbsp; </span>The dashed vertical lines that lead to the final BXD RI lines involve 21 full sib matings (about 7 years of breeding). Some lines die out during inbreeding. For example, there is no longer any BXD3 strain.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>1. Over the last decade, our group (Lu Lu and Rob Williams) and Jeremy Peirce and Lee Silver at Princeton have enlarged Ben TaylorÕs set. There are now just over 80 BXD strains. They have all been genotyped using about 13,700 markers (SNPs and microsatellites). These markers are used to define the ÒblueÓ and ÒredÓ regions of the chromosomes as shown in the figure above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. Chromosomes of RI GRPs usually have about 4 times as many recombinations as those of F2 animals. However, unlike an F2, both chromosomes of an RI are identical. Therefore, 50 RI strains contain as many recombinations as 100 F2 animals.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. BXD43 through BXD100 were generated using a special method that resulted in a further doubling of the average recombination density per chromosome. The entire set of 80 BXDs therefore contains as many recombinations as about 260 F2 animals.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'><i>Genetic versus Physical maps for App expression</i></span><span style='font-family: Verdana;font-size:73%;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:117%;color:#E9EB5D'><i>The difference between genetic and physical scale is analogous to measuring the </i></span></span></layer><script>
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+</script><span style='text-align:left'><span style='font-size:117%;color:#E9EB5D'><i>separation between New York and Boston in either travel hours or kilometers</i></span><span style='font-size:150%;color:#E9EB5D'><i>.</i></span><span style='font-size: 167%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></layer></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The map on the top has an X-axis scale based on frequency of recombinations events between markers (B to D transitions, see slide 19 for a color-coded example). These so-called genetic maps are scaled in centimorgan (recombinations per 100 gametes). In contrast, the physical map shown below the genetic map has an X-axis scale based on DNA length measured in nucleotides or base-pairs. Notice the large difference between the two maps in the size of Chr 19 (large on the genetic scale but small on the physical scale).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Also notice the large difference in the width of the chromosome 7 QTL peak. In mice, recombinations occur with higher frequency toward the telomeric side (right side) of each chromosome. As a result, genetic maps are stretched out more toward the telomere relative to a physical map. The QTL on distal Chr 7 is therefore actually more precisely mapped than might appear looking at the genetic map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The physical scale is becoming more useful than the genetic scale primarily because many other data types can be easily superimposed on a physical map. You will see more examples in the next several slides.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>This slide is illustrates two major types of QTLs that modulate variability in transcript-relative steady state abundance.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>1. cis QTLs are defined as QTLs that are closely linked to the gene whose transcript is the measured trait. For example, a polymorphism in the promoter that affects binding of a transcription factor. However, cis QTLs can be far upstream or downstream polymorphisms in enhancers or may be in 3Õ UTR binding sites that affect message stability.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>2. trans QTLs map far enough away from the location of the gene that gives rise to the transcript that is being measured so that we can be fairly certain that the QTL is not in the gene itself. The most blatant type of trans QTL would be a polymorphism in a transcription factor. But in the majority of cases, the trans QTLs can be far removed in a mechanistic sense from the actual events modulating transcript abundance. That is why there are three overlapping arrows in the figure.<span style="mso-spacerun: yes">&nbsp; </span>The way in which an upstream polymorphism influences a downstream difference in mRNA abundance can be indirect. Effects can:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>a.<span style="mso-spacerun: yes">&nbsp; </span>cross tissue types (a polymorphic liver enzyme may affect CNS gene expression)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>b.<span style="mso-spacerun: yes">&nbsp; </span>cross time (the modulator is only expressed for one day during development but has permanent effects in adults)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>c.<span style="mso-spacerun: yes">&nbsp; </span>may be contingent on environmental factors (heat shock may trigger the expression of a polymorphic factor that affects mRNA abundance).</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.08 * g_height; mywidth = 1.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'>Physical map for distal chromosome 7</span><span style='font-family:Verdana;font-size: 73%;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </layer></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0.05 * g_width; myheight = 0.06 * g_height; mywidth = 0.94 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.04 * g_height; mywidth = 0.92 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><span style='font-size:167%;color:#E9EB5D'>Distal Chr 7 from ~120 and 132 Mb may modulate</span><span style='font-size:167%;color:#E9EB5D'><i> App</i></span><span style='font-size:200%;color:#E9EB5D;mso-special-format: lastCR;display:none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Physical map of variation in App expression in brain on distal Chr 7 (a blow up of the whole-genome map on the previous slide).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Verdana size=3> You can now see that the X-axis is on a physical scale of megabases (Mb). The QTL peak is roughly between 120 and 132 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. The small irregular colored blocks and marks toward the top of the map mark the locations of genes superimposed on the physical map. Neighboring genes are offset slightly in the vertical axis for display purpose. Note one region of very high gene density from about 120 to 123 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. The orange hash marks along the X-axis represent the number of single nucleotide polymorphisms that distinguish the two parental strains (C57BL/6J and DBA/2J) from each other. We call this the SNP seismograph track (see Glossary for more details). Regions with low numbers of SNPs have closely matched sequences and are less likely to contain QTLs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4. As before, the thin red line shows the additive effect size. By convention the positive values signify the D alleles are associated with higher expression of App in this region of Chr 7 than the B alleles. The maximum effect size is about +0.20 log2 expression units per D allele. The differences been the BB and DD genotypes (BB and DD because each strain has two alleles; one per chromosome) is therefore about 2^0.4 = 1.32 or a 32% increment in DD relative to BB at this locus.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5. If you scroll just under the Physical Map you will see text that reads ÒDISPLAY from XXX Mb TO YYY MbÉ..Ó<span style="mso-spacerun: yes">&nbsp; </span>These physical maps are zoomable, a feature we will exploit to evaluate candidate genes in this QTL interval.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+</script><script language=JavaScript><!--
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+
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+</script> </body> </html> \ No newline at end of file
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Please bring the Trait Data and Analysis window to the front and look for the Interval Mapping button. Confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Select Mapping Scale is set to GENETIC</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Options: Permutation test YES<span style="mso-spacerun: yes">&nbsp; </span>(2000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Options: Bootstrap test YES (2000 is the default number)</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ mytop = -0.01 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.93 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.06 * g_height; mywidth = 0.91 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-line-spacing:"-358 0 -1"'><span style='font-family:Verdana;font-size:64%'>Evaluating candidate genes</span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ mytop = 0.03 * g_height; myleft = 0.69 * g_width; myheight = 0.17 * g_height; mywidth = 0.33 * g_width;
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.31 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center'><span style='font-size:200%;color:#E9EB5D'><i>Right position<br> </i></span></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.31 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.12 * g_height; mywidth = 0.21 * g_width;
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center'><span style='font-size:200%;color:#E9EB5D'><i>candidates</i></span></div> </layer></div> </layer></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Evaluating candidate genes (CHECKED BOXES) responsible for variability in APP expression:</b></font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>A large number of genes are usually in the QTL interval and are therefore POSITIONAL CANDIDATES, but they will differ greatly in their biological and bioinformatic plausibility. Assume that the QTL has been located between 119 and 131 Mb (12 Mb). There will typically be 12 to 15 genes per Mb, so we might need to evaluate several hundred positional candidates. In this particular case there are about 100 known genes in this interval. Eight of these are highlighted in the table above with check marks in the boxes to the left.<span style="mso-spacerun: yes">&nbsp; </span>We need to highlight and objectively score the biologically relevant subset of all 100 positional candidate genes. We could look through gene ontologies and expression levels to help us shorten the list. An alternate way available using WebQTL is to generate a list of those genes in this interval that have transcripts that co-vary in expression with App expression. That is what the table shows.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. To replicate this table go back to the Trait Data and Analysis Form. Choose to sort correlations by POSITION and select RETURN = 500. Then scroll down the list to Chr 7 and review the subset of positional candidates that share expression with App. You should see a list similar to that shown above. Gtf3c1 is a good biological candidate and has a high covariation in expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>2. Caveat:<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>Of course, the gene or genes that control App expression may not be in this list. A protein coding difference might be the ultimate cause of variation in App transcript level and the expression covariation might be close to zero. Our list may also simply be missing the right transcript since the microarray is not truly comprehensive. Furthermore, even if the list contains the QTL gene, an expression difference may only have been expressed early in development or even in another tissue such as liver. While it is important to recognize these caveats, it is equally important to devise a rational way to rank candidates given existing data. Coexpression is one of several criteria used to evaluate positional candidates. We will see others in the next slide.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>3. We can also assess the likelihood that candidates contain functional polymorphism in promoters and enhancers that affect their expression simply by mapping the transcripts of all candidate genes to see if they Òmap backÓ to the location of gene itself. A transcript that maps to its own location is referred to as a cis QTL. We essentially ask: Which of the transcripts listed in the Correlation Table above (from Gtf3c1 to Zranb1) has variation in expression that maps to Chr 7 at about 120 Mb?<span style="mso-spacerun: yes">&nbsp; </span>The logic of this search is that if a gene controls the level of its own expression it is also much more likely to generate other downstream effects. The Gtf3c1 transcript is a weak cis QTL with a local LRS maximum of about 7.0 (D alleles are high). That is just about sufficient to declare it to be a cis QTL. [No whole genome correction is required and a point-wise p-value of 0.05 is the appropriate test. A p-value of 0.05 is roughly equivalent to an LRS of 6.0 (LOD = 1.3).]</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>This is a major output type: a so-called full-genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromosomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans at almost 0 megabases). The blue labels along the bottom of the figure list a subset of the 3795 markers that were used in mapping.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The thick blue wavy line running across chromosomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of all markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The red line and the red axis to the far right provides an estimate of the effect that a QTL has on expression of App (this estimate of the so-called additive effect tends to be too high). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher trait values. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and those that have the D/D genotype at a specific marker. For example, on distal Chr 7 the red line peaks at a value of about 0.2. That means that this region of chromosome 2 is responsible for a 0.4 unit expression difference between B/B strains and the D/D strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides a method to evaluate whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But using the bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 3 and Chr 7 under the LRS peaks. That is somewhat reassuring. But notice that a substantial number of bootstrap are scattered around on other chromosomes. About 30% of the bootstrap resamples have a peak on Chr 7. That is pretty good, but does makes us realize that the sample we are working with is still quite small and fragile.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The horizontal dashed lines at 10.5 and 17.3 are the likelihood ratio statistic (LRS) values associated with the suggestive and significant genome-wide probabilities that were established by permutations of phenotypes across genotypes. We shuffle randomly 2000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 17.3. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 3 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations. Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. If you click on the body of the map, say on the blue line, then you will generate a view on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. If you click on a marker symbol, then you will generate a new Trait data and Analysis window with the genotypes loaded into the window just like any other trait. More on this in Section 3.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>4. You can drag these maps off of the browser window and onto your desktop. They will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>5. There is also an option at the bottom of the page to download a 2X higher resolution image of this plot for papers and presentations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>6. You can also download the results of the analysis in a text format</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>An even higher blow-up of part of the Chr 7 physical map of variation in App expression in brain.<span style="mso-spacerun: yes">&nbsp; </span>The QTL region actually extends from about 119 to 129.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. As mentioned in the previous slide another important approach to ranking candidates is based on the number of sequence variants that distinguish the parental strains. If we were sure that the sequences of the gene, its promoter, and its enhancers were identical between the strains then we could discount--but not eliminate--that gene as a candidate. The Gtf3c1 candidate almost falls into this category: of 663 known SNPs in and around this gene, only four differ between C57BL/6J and DBA/2J. Gtf3c1 is essentially identical-by-descent in these strains and is a less likely candidate. In contrast, if the two alleles of the gene have dozens of functional variants in exons, promoters, enhancers, and splice sites, then it becomes a higher priority candidate.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Of course it only takes a single critical sequence variant to generate downstream effects. The argument above is really about the prior probabilities. Where would you place your bets given the information at hand?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2.<span style="mso-spacerun: yes">&nbsp; </span>If you scroll down the INTERVAL ANALYST you will find that Ctbp2 is a particularly interesting candidate that contains lots of SNPs (n = 75 and a SNP density of 0.55 SNP/Kb). Ctbp2 is also closer to our QTL peak than was Gtf3c1. Not only does Ctbp2 contain lots of SNPs but it is also is associated with a powerful cis QTL with an LRS of 24.2 (divide by 4.61 to get the equivalent LOD score of 5.25).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3.<span style="mso-spacerun: yes">&nbsp; </span>At this high magnification, individual genes are distinct. They are color coded by their density of SNPs. Bright orange represents those genes that have a high SNP density (C57BL/6J versus DBA/2J), black represents genes with low SNP density. Roll the cursor over a gene block and its name will pop up, along with information on exon number.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4.<span style="mso-spacerun: yes">&nbsp; </span>Beneath the physical map you will find an INTERVAL ANALYST table that lists information on known genes in the region on which you have zoomed the Physical Map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5.<span style="mso-spacerun: yes">&nbsp; </span>As always: error-checking is important. Some genes may be missing from the Interval Analyst (recent additions or errors of omission). In this case the Zranb1 gene that is located just proximal to Ctbp2 is not listed in the INTERVAL ANALYST. Double-check the interval using the Genome Browser links (blue and beige horizontal bars) at the top of the PHYSICAL MAP.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The map on the top has an X-axis scale based on frequency of recombinations events between markers (B to D transitions, see slide 19 for a color-coded example). These so-called genetic maps are scaled in centimorgan (recombinations per 100 gametes). In contrast, the physical map shown below the genetic map has an X-axis scale based on DNA length measured in nucleotides or base-pairs. Notice the large difference between the two maps in the size of Chr 19 (large on the genetic scale but small on the physical scale).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Also notice the large difference in the width of the chromosome 7 QTL peak. In mice, recombinations occur with higher frequency toward the telomeric side (righ sidet) of each chromosome. As a result, genetic maps are stretched out more toward the telomere relative to a physical map. The QTL on distal Chr 7 is therefore actually more precisely mapped than might appear looking at the genetic map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The physical scale is becoming more useful than the genetic scale primarily because many other data types can be easily superimposed on a physical map. You will see more examples in the next several slides.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'>Evaluating </span><span style='font-family:Verdana;font-size:64%'><i>Ctbp2</i></span><span style='font-family:Verdana;font-size:64%'> as a candidate QTL for </span><span style='font-family:Verdana;font-size:64%'><i>App</i></span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%;color:#E9EB5D'><i> the </i></span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%;color:#E9EB5D'><i> the App QTL </i></span></span></layer><script>
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+</script></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This slide illustrates one reason why Ctbp2 should be considered a high priority positional candidate gene that may modulate the expression level of App.<span style="mso-spacerun: yes">&nbsp; </span>Ctbp2 is a strong cis QTL in some brain regions (here the data are taken from the striatum).<span style="mso-spacerun: yes">&nbsp; </span>If Ctbp2 contains variants that modulate its own expression then these expression differences may produce many downstream effects. Of course, we now want to know much more about the known biology of Ctbp2. What kind of gene is it? To begin to answer that question we can use a number of resources listed in the LINKS page.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Verdana size=3> The App QTL is bimodal. Perhaps there are actually two causal factors in this region--one close to 123 Mb and the other close to 127 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. The precision of QTL mapping depends on several factors, including the effect size and interactions among QTLs modulating a trait, the number of genetic individuals that are studied, and the distribution of recombinations in the study population.<span style="mso-spacerun: yes">&nbsp; </span>In the case above, the QTL(s) are likely to be confined to the interval from 120 to 132 Mb. The bootstrap test (yellow bars shown in some of the previous slides) can be usual for estimating the consistency of QTL peaks.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
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+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0024_image109.png b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0024_image109.png
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0024_notes_pane.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0024_notes_pane.htm
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Physical map of variation in App expression in brain on distal Chr 7 (a blow up of the whole-genome map on the previous slide).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=2>1.</font><font face=Verdana size=2> You can now see that the X-axis is on a physical scale of megabases (Mb). The QTL peak is roughly between 120 and 132 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. The small irregular colored blocks and marks toward the top of the map mark the locations of genes superimposed on the physical map. Neighboring genes are offset slightly in the vertical axis for display purpose. Note one region of very high gene density from about 120 to 123 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. The orange hash marks along the X-axis represent the number of single nucleotide polymorphisms that distinguish the two parental strains (C57BL/6J and DBA/2J) from each other. We call this the SNP seismograph track (see Glossary for more details). Regions with low numbers of SNP have closely matched sequences and are less likely to contain QTLs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>4. As before, the thin red line shows the additive effect size. By convention the positive values signify the D alleles are associated with higher expression of App in this region of Chr 7 than the B alleles. The maximum effect size is about +0.20 log2 expression units per D allele. The differences been the BB and DD genotypes (BB and DD because each strain has two alleles; one per chromosome) is therefore about 2^0.4 = 1.32; or a 32% increment in DD relative to BB at this locus.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>5. If you scroll just under the Physical Map you will see text that reads ÒDISPLAY from XXX Mb TO YYY MbÉ..Ó<span style="mso-spacerun: yes">&nbsp; </span>These physical maps are zoomable, a feature we will exploit to evaluate candidate genes in this QTL interval.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'>Evaluating </span><span style='font-family:Verdana;font-size:64%'><i>Ctbp2</i></span><span style='font-family:Verdana;font-size:64%'> using other resources</span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.19 * g_width;
+yy = 0.4 * g_height;
+ht = 0.54 * g_height;
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+document.write(
+'<img border=0 src="slide0011_image088.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Ctbp2 should also be considered a high priority biological candidate gene responsible for modulating App expression levels. The C-terminal binding protein 2 is a transcriptional co-repressor also known as Ribeye.</font><font face=Times-Roman size=3> The gene produces two transcripts encoding distinct proteins. The short form is a transcriptional repressor that binds a Pro-X-Asp-Leu-Ser peptide motif and interacts with several transcription factors including EVI1, ZFPM1, and ZFHX1A (aka TCF8, deltaEF1). The longer isoform is a major component of specialized synapses in photoreceptors. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. To find out more about CTBP2 protein and the Ctbp2 gene, link to iHOP at http://www.pdg.cnb.uam.es/UniPub/iHOP/ and type in CTBP2</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Try Arrowsmith at http://arrowsmith.psych.uic.edu/cgi-test/arrowsmith_uic/pubsmith.cgi</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. Both APP and CTBP2 are involved in oxidoreducatase activity or Notch signaling. To establish this common gene ontology visit NCBI<span style="mso-spacerun: yes">&nbsp; </span>http://www.ncbi.nih.gov/entrez/query.fcgi?db=gene and enter each gene symbol.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. You can get interesting hints regarding Ctbp2 expression partners by examining the genetic correlations between Ctbp2 probe set 1422887_a_at and all other transcripts on the M430 Affymetrix array. Use the Striatum data set because we already know from previous work (the previous slide) that this gene is a cis QTL.<span style="mso-spacerun: yes">&nbsp; </span>You should be able to show that Ctbp2 and Notch3 have antagonistic expression patterns in striatum. The negative genetic correlation with E2f4 is even stronger. The transcript also has a high positive genetic correlation with Rdh14. Of particular interest with respect to APP protein processing, Ctbp2 covaries positively with Bace2 (the transcript of the beta site APP-cleaving enzyme 2).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0025_image112.png b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0025_image112.png
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+}
+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Evaluating candidate genes (CHECKED BOXES) responsible for variability in APP expression:</b></font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>A large number of genes are usually in the QTL interval and are therefore POSITIONAL CANDIDATES, but they will differ greatly in their biological and bioinformatic plausibility. Assume that the QTL has been located between 119 and 131 Mb (12 Mb). There will typically be 12 to 15 genes per Mb, so we might need to evaluate several hundred positional candidates. In this particular case there are about 100 known genes in this interval. Eight of these are highlighted in the table above with check marks in the boxes to the left.<span style="mso-spacerun: yes">&nbsp; </span>We need to highlight and objectively score the biologically relevant subset of all 100 positional candidate genes. We could look through gene ontologies and expression levels to help us shorten the list. An alternate way available using WebQTL is to generate a list of those genes in this interval that have transcripts that co-vary in expression with App expression. That is what the table shows.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. To replicate this table go back to the Trait Data and Analysis Form. Choose to sort correlations by POSITION and select RETURN = 500. Then scroll down the list to Chr 7 and review the subset of positional candidates that share expression with App. You should see a list similar to that shown above. Gtf3c1 is a good biological candidate and has a high covariation in expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>2. Caveat:<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>Of course, the gene or genes that control App expression may not be in this list. A protein coding difference might be the ultimate cause of variation in App transcript level and the expression covariation might be close to zero. Our list may also simply be missing the right transcript since the microarray is not truly comprehensive. Furthermore, even if the list contains the QT gene, an expression difference may only have been expressed early in development or even in another tissue such as liver. While it is important to recognize these caveats, it is equally important to devise a rational way to rank candidates given existing data. Coexpression is one of several criteria used to evaluate positional candidates. We will see others in the next slide.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>3. We can also assess the likelihood that candidates contain functional polymorphism in promoters and enhancers that affect their expression simply by mapping the transcripts of all candidate genes to see if they Òmap backÓ to the location of gene itself. A transcript that maps to its own location is referred to as a cis QTL. We essentially ask: Which of the the transcripts listed in the Correlation Table above (from Gtf3c1 to Zranb1) has variation in expression that maps to Chr 7 at about 120 Mb?<span style="mso-spacerun: yes">&nbsp; </span>The logic of this search is that if a gene controls the level of its own expression it is also much more likely to generate other downstream effects. The Gtf3c1 transcript is a weak cis QTL with a local LRS maximum of about 7.0 (D alleles are high). That is just about sufficient to declare it to be a cis QTL. [No whole genome correction is required and a point-wise p-value of 0.05 is the appropriate test. A p-value of 0.05 is roughly equivalent to an LRS of 6.0 (LOD = 1.3).]</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:nobullet;text-align:left;font-family:"Gill Sans"; font-weight:normal;font-style:normal;text-decoration:none;text-shadow:none; text-effect:none;mso-fareast-hint:no;layout-flow:horizontal;color:#FBFDB8; font-size:213%;mso-text-raise:0%;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0'><span style='mso-special-format:nobullet;display: none;color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'>Summary of Part 2</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
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+</script> <div> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.04 * g_height; mywidth = 0.87 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.04 * g_height; mywidth = 0.84 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:numbullet; position:absolute;left:-2.96%;font-family:Arial'>1.</span></span><span style='font-family:"Gill Sans";font-size:167%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>Covered the basics of QTL analysis and mapping.<br> </span></span></layer></div> </layer><script>
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+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.04 * g_height; mywidth = 0.84 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.04 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'>the additive effect function, the bootstrap bars, and the permutation </span></span></layer><script>
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+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.04 * g_height; mywidth = 0.86 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.04 * g_height; mywidth = 0.84 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.04 * g_height; mywidth = 0.89 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'>What does a QTL signify? A good QTL is a claim that a particular </span></span></layer><script>
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+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'>chromosomal region contains a causal source of variation in the phenotype. </span></span></layer><script>
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+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'>The importance of this hypothesis depends on the quality and relevance of the </span></span></layer><script>
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+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'>phenotype and the statistical strength of the QTL. As usual, test and be </span></span></layer><script>
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+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:167%; color:#E9EB5D;mso-color-index:3'>skeptical.</span></span></layer></div> </layer></div> </LAYER>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Even higher blow-up of part of the Chr 7 physical map of variation in App expression in brain.<span style="mso-spacerun: yes">&nbsp; </span>The QTL region actually extends from about 119 to 129.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. As mentioned in the previous slide another important approach to ranking candidates is based on the number of sequence variants that distinguish the parental strains. If we were sure that the sequences of the gene, its promoter, and its enhancers were identical between the strains then we could discount--but not eliminate--that gene as a candidate. The Gtf3c1 candidate almost falls into this category: of 663 known SNPs in and around this gene, only four differ between C57BL/6J and DBA/2J. Gtf3c1 is essentially identical-by-descent in these strains and is a less likely candidate. In contrast, if the two alleles of the gene have dozens of functional variants in exons, promoters, enhancers, and splice sites, then it becomes a higher priority candidate.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Of course it only takes a single critical sequence variant to generate downstream effects. The argument above is really about the prior probabilities. Where would you place your bets given the information at hand?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2.<span style="mso-spacerun: yes">&nbsp; </span>If you scroll down the INTERVAL ANALYST you will find that Ctbp2 is a particularly interesting candidate that contains lots of SNPs (n = 75 and a SNP density of 0.55 SNP/Kb). Ctbp2 is also closer to our QTL peak than was Gtf3c1. Not only does Ctbp2 contain lots of SNPs but it is also is associated with a powerful cis QTL with an LRS of 24.2 (divide by 4.61 to get the equivalent LOD score of 5.25).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3.<span style="mso-spacerun: yes">&nbsp; </span>At this high magnification, individual genes are distinct. They are color coded by their density of SNPs. Bright orange represents those genes that have a high SNP density (C57BL/6J versus DBA/2J), black represents genes with low SNP density. Roll the cursor over a gene block and its name will pop up, along with information on exon number.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>4.<span style="mso-spacerun: yes">&nbsp; </span>Beneath the physical map you will find an INTERVAL ANALYST table that lists information on known genes in the region on which you have zoomed the Physical Map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>5.<span style="mso-spacerun: yes">&nbsp; </span>As always: error-checking is important. Some genes may be missing from the Interval Analyst (recent additions or errors of omission). In this case the Zranb1 gene that is located just proximal to Ctbp2 is not listed in the INTERVAL ANALYST. Double-check the interval using the Genome Browser links (blue and beige horizontal bars) at the top of the PHYSICAL MAP.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.08 * g_height; mywidth = 1.01 * g_width;
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>2. Do </span><span style='font-size:233%;color:#E9EB5D'><i>App</i></span><span style='font-size: 233%;color:#E9EB5D'> and </span><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2</i></span><span style='font-size:233%;color:#E9EB5D'> expression share any </span></span></layer><script>
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+}
+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>This slide illustrates one reason why Ctbp2 should be considered a high priority positional candidate gene that may modulate the expression level of App.<span style="mso-spacerun: yes">&nbsp; </span>Ctbp2 is a strong cis QTL in some brain regions (here the data are taken from the striatum).<span style="mso-spacerun: yes">&nbsp; </span>If Ctbp2 contains variants that modulate its own expression then these expression differencess may produce many downstream effects. Of course, we now want to know much more about the known biology of Ctbp2. What kind of gene is it? To begin to answer that question we can use a number of resources listed in the LINKS page.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=2>1.</font><font face=Verdana size=2> The App QTL is bimodal. Perhaps there are actually two causal factors in this region--one close to 123 Mb and the other close to 127 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. The precision of QTL mapping depends on several factors, including the effect size and interactions among QTLs modulating a trait, the number of genetic individuals that are studied, and the distribution of recombinations in the study population.<span style="mso-spacerun: yes">&nbsp; </span>In the case above, the QTL(s) are likely to be confined to the interval from 120 to 132 Mb. The bootstrap test (yellow bars shown in some of the previous slides) can be usual for estimating the consiistency of QTL peaks.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><span style='font-family:"Gill Sans";font-size:300%; color:#E9EB5D'>kmanly@utmem.edu</span></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0028_image123.png b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0028_image123.png
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Ctbp2 should also be considered a high priority biological candidate gene responsible for modulating App expression levels. The<span style="mso-spacerun: yes">&nbsp; </span>C-terminal binding protein 2 is a transcriptional co-repressor also known as Ribeye.</font><font face=Times-Roman size=2> The gene produces two transcripts encoding distinct proteins. The short form is a transcriptional repressor that binds a Pro-X-Asp-Leu-Ser peptide motif common to adenoviral oncoprotein E1a and a related motif in BKLF. This short form also interacts<span style="mso-spacerun: yes">&nbsp; </span>with several transcription factors including EVI1, ZFPM1, and<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>ZFHX1A (aka TCF8, deltaEF1). The longer isoform is a major component of specialized synapses in photoreceptors. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>1. To find out more about CTBP2 protein and the Ctbp2 gene, link to iHOP at http://www.pdg.cnb.uam.es/UniPub/iHOP/ and type in CTBP2</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Try Arrowsmith at http://arrowsmith.psych.uic.edu/cgi-test/arrowsmith_uic/pubsmith.cgi</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. Both APP and CTBP2 are involved in oxidoreducatase activity or Notch signalling. To estabilish this common gene ontology visit NCBI<span style="mso-spacerun: yes">&nbsp; </span>http://www.ncbi.nih.gov/entrez/query.fcgi?db=gene<span style="mso-spacerun: yes">&nbsp; </span>and enter each gene symbol.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. You can get intersting hints regarding Ctbp2 expression partners by examining the genetic correlations between Ctbp2 probe set 1422887_a_at and all other transcripts on the M430 Affymetrix array. Use the Striatum data set because we already know from previous work (the previous slide) that this gene is a cis QTL.<span style="mso-spacerun: yes">&nbsp; </span>You should be able to show that Ctbp2 and Notch3 have antagonistic expression patterns in striatum. The negative genetic correlation with E2f4 is even stronger. The transcript also has a high positive genetic correlation with Rdh14. Of particualr interest with respect to APP protein processing, Ctbp2 covaries positiviely with Bace2 (the transcript of the beta site APP-cleaving enzyme 2).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.9 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:nobullet;text-align:left;font-family:"Gill Sans"; font-weight:normal;font-style:normal;text-decoration:none;text-shadow:none; text-effect:none;mso-fareast-hint:no;layout-flow:horizontal;color:#FBFDB8; font-size:213%;mso-text-raise:0%;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 348;mso-text-indent-alt:56;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 348;mso-text-indent-alt:56'><span style='mso-special-format:nobullet; display:none;color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'><i>App</i></span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'> and </span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'><i>Atcay</i></span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'> transcript scatterplot </span></div> </layer></div> </layer><script>
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+//-->
+</script><script language=JavaScript><!--
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+
+}
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0044.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0044.htm
new file mode 100755
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+wd = g_width;
+document.write(
+'<img src="master03_background.png" height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.14 * g_height; mywidth = 0.99 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.96 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:nobullet;text-align:left;font-family:"Gill Sans"; font-weight:normal;font-style:normal;text-decoration:none;text-shadow:none; text-effect:none;mso-fareast-hint:no;layout-flow:horizontal;color:#FBFDB8; font-size:213%;mso-text-raise:0%;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 348;mso-text-indent-alt:56;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 348;mso-text-indent-alt:56'><span style='mso-special-format:nobullet; display:none;color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'><i>App</i></span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'> transcript and eight of its neighbors</span></div> </layer></div> </layer><script>
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+</script> <div><script language=JavaScript><!--
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+yy = 0.09 * g_height;
+ht = 0.9 * g_height;
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+//-->
+</script></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>A group of traits from many different databases can be selected and brought together for joint analysis. In this case all of the content of the BXD SELECTIONS is from a single BRAIN database, the top 20 neighbors of the App transcript from the Correlation Results table. Eight of these neighbors plus App is shown in the slide.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Helvetica size=3>All of items in the BXD SELECTIONS were selected using the SELECT ALL button</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. The buttons at the top (and bottom) of this page can do some cool stuff. We will work with NETWORK GRAPH first.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>3. Think of the SELECTIONS as your shopping cart. You go to different aisles in the supermarket to acquire different types of items of interest. These could include transcripts, classical phenotypes (longevity, brain weight, prepulse inhibition, iron levels in midbrain). ÒChecking outÓ in this case involves doing some analysis with the items in the cart.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>4. Different tools handle different numbers of items. Most will handle up to 100 traits.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0045.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0045.htm
new file mode 100755
index 00000000..4e705866
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0045.htm
@@ -0,0 +1,118 @@
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+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
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+<script language=JavaScript>
+function font_size() {
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+}
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+}
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+document.captureEvents(Event.KEYPRESS);
+document.onkeypress = _KPH ;
+document.onmousedown = _KPH ;
+ parent.base.highlite();
+</script><script language=JavaScript><!--
+function DoOnLoad() {
+ LoadNavSld("slide0045.htm");
+}//-->
+</script> </head> <body onresize="restore()" onload="DoOnLoad()" style='font-size:&{font_size()};' bgcolor="#000000"> <script>
+
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+
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+ht = g_height;
+wd = g_width;
+document.write(
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+//-->
+</script></div> </layer><script>
+ mytop = 0.82 * g_height; myleft = 0.04 * g_width; myheight = 0.18 * g_height; mywidth = 0.96 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div></div> </layer><script>
+ mytop = -0.02 * g_height; myleft = 0 * g_width; myheight = 0.12 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.02 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 1.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+</script> <div><script language=JavaScript><!--
+xx = 0.01 * g_width;
+yy = 0.37 * g_height;
+ht = 0.62 * g_height;
+wd = 0.97 * g_width;
+document.write(
+'<img border=0 src="slide0014_image029.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.11 * g_height; myleft = 0.01 * g_width; myheight = 0.25 * g_height; mywidth = 0.79 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.01 * g_width;
+yy = 0.11 * g_height;
+ht = 0.25 * g_height;
+wd = 0.79 * g_width;
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+//-->
+</script></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.81 * g_width; myheight = 0.09 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.81 * g_width;
+yy = 0.1 * g_height;
+ht = 0.09 * g_height;
+wd = 0.18 * g_width;
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+//-->
+</script></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Output of the Network Graph. Warm colors (orange and red) are positive correlations above 0.5 whereas cool colors (green and blue) are negative correlations. Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Helvetica size=3> All of the nodes (gene/transcripts) on this graph are clickable.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. For this graph the App expression values have ÒrevertedÓ to their pre-Windsorized values.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>3. To generate this graph, we used the default setting:<span style="mso-spacerun: yes">&nbsp; </span>Size of 16 by 16 inches; Gene Symbols; Don't Show Correlations; Use curved lines (aka ÒedgesÓ).</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0046.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0046.htm
new file mode 100755
index 00000000..93429543
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0046.htm
@@ -0,0 +1,104 @@
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+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Jul-18-05> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=Javascript src=script.js></script>
+<script language=JavaScript>
+function font_size() {
+ var min = window.innerWidth/20;
+ if ( min > window.innerHeight/20 )
+ min = window.innerHeight/20;
+
+ return min+"pt";
+}
+var SoundEnabled = navigator.plugins && navigator.plugins["LiveAudio"];
+function restore() {
+ self.location.reload();
+}
+function set_size() {
+ document.SlideLayer.width = window.innerWidth;
+ document.SlideLayer.height = window.innerHeight;
+}
+document.captureEvents(Event.MOUSEDOWN);
+document.captureEvents(Event.KEYPRESS);
+document.onkeypress = _KPH ;
+document.onmousedown = _KPH ;
+ parent.base.highlite();
+</script><script language=JavaScript><!--
+function DoOnLoad() {
+ LoadNavSld("slide0046.htm");
+}//-->
+</script> </head> <body onresize="restore()" onload="DoOnLoad()" style='font-size:&{font_size()};' bgcolor="#000000"> <script>
+
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+</script> <div><script language=JavaScript><!--
+ht = g_height;
+wd = g_width;
+document.write(
+'<img src="master03_background.png" height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.14 * g_height; mywidth = 0.93 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.91 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:nobullet;text-align:left;font-family:"Gill Sans"; font-weight:normal;font-style:normal;text-decoration:none;text-shadow:none; text-effect:none;mso-fareast-hint:no;layout-flow:horizontal;color:#FBFDB8; font-size:213%;mso-text-raise:0%;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0'><span style='mso-special-format:nobullet;display: none;color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'>Correlations of </span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'><i>App</i></span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'> with classical traits</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.04 * g_width;
+yy = 0.28 * g_height;
+ht = 0.7 * g_height;
+wd = 0.78 * g_width;
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+//-->
+</script></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0.04 * g_width; myheight = 0.19 * g_height; mywidth = 0.33 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.04 * g_width;
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+//-->
+</script></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Going back to the Trait Data and Analysis Form window, we have computed the correlations between strain variation in App expression level and other classical phenotypes that have already been measured in many of the same BXD strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Helvetica size=3>The number of common strains varies widely--in this case from 14 to 23 strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. We can add these traits (four are selected) to our BXD SELECTIONS window.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0047.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0047.htm
new file mode 100755
index 00000000..e7568e44
--- /dev/null
+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0047.htm
@@ -0,0 +1,93 @@
+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
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+}
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+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:nobullet;text-align:left;font-family:"Gill Sans"; font-weight:normal;font-style:normal;text-decoration:none;text-shadow:none; text-effect:none;mso-fareast-hint:no;layout-flow:horizontal;color:#FBFDB8; font-size:213%;mso-text-raise:0%;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0'><span style='mso-special-format:nobullet;display: none;color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'>Network Graph of </span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'><i>App</i></span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'> with classical traits</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>We have computed the Network Graph, now using other types of traits.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Saline Hot Plate Latency is the green node labeled 10020.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Freezing (fear) is the green node labeled 10447.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Notes:</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+ mytop = 0.01 * g_height; myleft = 0.03 * g_width; myheight = 0.14 * g_height; mywidth = 0.93 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.91 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:nobullet;text-align:left;font-family:"Gill Sans"; font-weight:normal;font-style:normal;text-decoration:none;text-shadow:none; text-effect:none;mso-fareast-hint:no;layout-flow:horizontal;color:#FBFDB8; font-size:213%;mso-text-raise:0%;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0'><span style='mso-special-format:nobullet;display: none;color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'>Summary of Part 1:</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.08 * g_width; myheight = 0.47 * g_height; mywidth = 1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <layer> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'></div> </layer> <script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.9 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.88 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:numbullet; position:absolute;left:-2.84%;font-family:Arial'>1.</span></span><span style='font-family:"Gill Sans";font-size:200%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>You have learned the basics about searching for traits<br> </span></span></layer></div> </layer><script>
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+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.83 * g_width;
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+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.83 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.88 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:numbullet; position:absolute;left:-2.85%;font-family:Arial'>4.</span></span><span style='font-family:"Gill Sans";font-size:200%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>You know how to review the basic statistics of a trait<br> </span></span></layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.91 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>traits using the Traits Correlation tool<br> </span></span></layer></div> </layer><script>
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+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.97 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>co-vary. </span></span></layer></div> </layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+}
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0050.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0050.htm
new file mode 100755
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+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.02 * g_height; mywidth = 0.29 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Times;font-size:117%; color:white;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.02 * g_height; myleft = -0.05 * g_width; myheight = 0.11 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.1 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-240 0 0";mso-margin-left-alt: 16;mso-text-indent-alt:16'><span style='font-family:Arial;font-size:267%; color:#E9EB5D'>How to make recombinant inbred strains (RI)</span><span style='font-family:Arial;font-size:150%;color:aqua;display:none'><br> </span></div> </layer><script>
+ mytop = 0.06 * g_height; myleft = 0.06 * g_width; myheight = 0.03 * g_height; mywidth = 0.92 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-160 0 0";mso-margin-left-alt: 16;mso-text-indent-alt:16'><span style='font-family:Arial;font-size:150%; color:aqua;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.28 * g_height; myleft = 0.45 * g_width; myheight = 0.01 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.45 * g_width;
+yy = 0.28 * g_height;
+ht = 0.01 * g_height;
+wd = 0.18 * g_width;
+document.write(
+'<img border=0 src="slide0019_image039.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.2 * g_height; myleft = 0.28 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.28 * g_width;
+yy = 0.2 * g_height;
+ht = 0.11 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image040.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.15 * g_height; myleft = 0.25 * g_width; myheight = 0.09 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>C57BL/6J (B)<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.15 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.2 * g_height; myleft = 0.3 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.3 * g_width;
+yy = 0.2 * g_height;
+ht = 0.11 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image041.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.15 * g_height; myleft = 0.62 * g_width; myheight = 0.09 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.15 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.15 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>DBA/2J (D)<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.2 * g_height; myleft = 0.66 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.66 * g_width;
+yy = 0.2 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image042.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.35 * g_height; myleft = 0.23 * g_width; myheight = 0.01 * g_height; mywidth = 0.5 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.23 * g_width;
+yy = 0.35 * g_height;
+ht = 0.01 * g_height;
+wd = 0.5 * g_width;
+document.write(
+'<img border=0 src="slide0019_image043.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.35 * g_height; myleft = 0.24 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.24 * g_width;
+yy = 0.35 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image044.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.35 * g_height; myleft = 0.4 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.4 * g_width;
+yy = 0.35 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image045.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.35 * g_height; myleft = 0.57 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.57 * g_width;
+yy = 0.35 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image046.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.35 * g_height; myleft = 0.74 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.74 * g_width;
+yy = 0.35 * g_height;
+ht = 0.03 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image047.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.42 * g_height; myleft = 0.05 * g_width; myheight = 0.12 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F1<br> </b></span></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format: lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.39 * g_height; myleft = 0.22 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.22 * g_width;
+yy = 0.39 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image048.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.39 * g_height; myleft = 0.38 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.38 * g_width;
+yy = 0.39 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image049.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.39 * g_height; myleft = 0.55 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.55 * g_width;
+yy = 0.39 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image050.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.39 * g_height; myleft = 0.72 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.72 * g_width;
+yy = 0.39 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image051.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.43 * g_height; myleft = 0.43 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.43 * g_width;
+yy = 0.43 * g_height;
+ht = 0.03 * g_height;
+wd = 0.11 * g_width;
+document.write(
+'<img border=0 src="slide0019_image052.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.6 * g_height; myleft = 0.2 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.2 * g_width;
+yy = 0.6 * g_height;
+ht = 0.03 * g_height;
+wd = 0.09 * g_width;
+document.write(
+'<img border=0 src="slide0019_image053.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.71 * g_height; myleft = 0.58 * g_width; myheight = 0.26 * g_height; mywidth = 0.2 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.03 * g_height; myleft = 0.01 * g_width; myheight = 0.22 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.15 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>20 generations </b></span></span></layer><script>
+ mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>brother-sister </b></span></span></layer><script>
+ mytop = 0.12 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>matings</b></span><span style='font-family:Arial;font-size: 233%;color:#FFFF99;display:none'><b><br> </b></span></span></layer></div> </layer><script>
+ mytop = 0.16 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-269 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:233%; color:#FFFF99;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.6 * g_height; myleft = 0.5 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.5 * g_width;
+yy = 0.6 * g_height;
+ht = 0.03 * g_height;
+wd = 0.09 * g_width;
+document.write(
+'<img border=0 src="slide0019_image054.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.62 * g_height; myleft = 0.54 * g_width; myheight = 0.28 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.54 * g_width;
+yy = 0.62 * g_height;
+ht = 0.28 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image055.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.61 * g_height; myleft = 0.79 * g_width; myheight = 0.03 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.79 * g_width;
+yy = 0.61 * g_height;
+ht = 0.03 * g_height;
+wd = 0.1 * g_width;
+document.write(
+'<img border=0 src="slide0019_image056.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.62 * g_height; myleft = 0.83 * g_width; myheight = 0.28 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.83 * g_width;
+yy = 0.62 * g_height;
+ht = 0.28 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image057.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.92 * g_height; myleft = 0.21 * g_width; myheight = 0.09 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD1</span><span style='font-family:Arial;font-size:150%;color:#484848;display:none'><br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#484848;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.92 * g_height; myleft = 0.51 * g_width; myheight = 0.09 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD2<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.92 * g_height; myleft = 0.8 * g_width; myheight = 0.05 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:"-173 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;mso-line-spacing:"-173 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>BXD80</span></div> </layer></div> </layer><script>
+ mytop = 0.9 * g_height; myleft = 0.64 * g_width; myheight = 0.17 * g_height; mywidth = 0.17 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.15 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-341 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:300%;color:#FFFF99'>+ É +<br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-341 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:300%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.62 * g_height; myleft = 0.04 * g_width; myheight = 0.12 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F2<br> </b></span></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-230 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format: lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.18 * g_width; myheight = 0.01 * g_height; mywidth = 0.75 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.18 * g_width;
+yy = 0.54 * g_height;
+ht = 0.01 * g_height;
+wd = 0.75 * g_width;
+document.write(
+'<img border=0 src="slide0019_image058.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.17 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.17 * g_width;
+yy = 0.54 * g_height;
+ht = 0.03 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image059.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.32 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.32 * g_width;
+yy = 0.54 * g_height;
+ht = 0.03 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image060.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.48 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.48 * g_width;
+yy = 0.54 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image061.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.62 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.62 * g_width;
+yy = 0.54 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image062.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.77 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.77 * g_width;
+yy = 0.54 * g_height;
+ht = 0.04 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image063.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.92 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.92 * g_width;
+yy = 0.54 * g_height;
+ht = 0.03 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image064.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.44 * g_height; myleft = 0.49 * g_width; myheight = 0.1 * g_height; mywidth = 0 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.49 * g_width;
+yy = 0.44 * g_height;
+ht = 0.1 * g_height;
+wd = 0 * g_width;
+document.write(
+'<img border=0 src="slide0019_image065.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.15 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.15 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image066.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.3 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.3 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image067.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.46 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.46 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image068.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.6 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.6 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image069.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.75 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.75 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image070.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = 0.9 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.9 * g_width;
+yy = 0.57 * g_height;
+ht = 0.11 * g_height;
+wd = 0.04 * g_width;
+document.write(
+'<img border=0 src="slide0019_image071.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.84 * g_height; myleft = 0.56 * g_width; myheight = 0.01 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.56 * g_width;
+yy = 0.84 * g_height;
+ht = 0.01 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image072.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.8 * g_height; myleft = 0.25 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.25 * g_width;
+yy = 0.8 * g_height;
+ht = 0.11 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image073.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.84 * g_height; myleft = 0.86 * g_width; myheight = 0.02 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.86 * g_width;
+yy = 0.84 * g_height;
+ht = 0.02 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image074.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.75 * g_height; myleft = 0.01 * g_width; myheight = 0.13 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'><b>BXD RI<br> </b></span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'><b>Strain set<br> </b></span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.12 * g_height; myleft = 0.14 * g_width; myheight = 0.06 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.14 * g_width;
+yy = 0.12 * g_height;
+ht = 0.06 * g_height;
+wd = 0.1 * g_width;
+document.write(
+'<img border=0 src="slide0019_image075.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.76 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.76 * g_width;
+yy = 0.1 * g_height;
+ht = 0.08 * g_height;
+wd = 0.14 * g_width;
+document.write(
+'<img border=0 src="slide0019_image076.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.2 * g_height; myleft = 0.17 * g_width; myheight = 0.13 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>fully<br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>inbred<br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.38 * g_height; myleft = 0.02 * g_width; myheight = 0.09 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>isogenic<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.54 * g_height; myleft = 0.01 * g_width; myheight = 0.13 * g_height; mywidth = 0.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>hetero-<br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>geneous<br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.74 * g_height; myleft = 0.29 * g_width; myheight = 0.21 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.19 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.15 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>Recombined </span></span></layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>chromosomes </span></span></layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>are needed for </span></span></layer><script>
+ mytop = 0.12 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>mapping<br> </span></span></layer></div> </layer><script>
+ mytop = 0.16 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-173 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.25 * g_width; myheight = 0.09 * g_height; mywidth = 0.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>female<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.64 * g_width; myheight = 0.09 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>male<br> </span></div> </layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.3 * g_height; myleft = 0.78 * g_width; myheight = 0.09 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:117%;color:#FFFF99'>chromosome pair</span><span style='font-family:Arial;font-size:150%;color:#FFFF99; display:none'><br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.15 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-173 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.31 * g_height; myleft = 0.65 * g_width; myheight = 0.02 * g_height; mywidth = 0.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.65 * g_width;
+yy = 0.31 * g_height;
+ht = 0.02 * g_height;
+wd = 0.12 * g_width;
+document.write(
+'<img border=0 src="slide0019_image077.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.86 * g_height; myleft = 0.03 * g_width; myheight = 0.14 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.12 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:#E9EB5D'>Inbred<br> </span></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:#E9EB5D'>Isogenic<br> </span></div> </layer><script>
+ mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:#E9EB5D'>siblings</span><span style='font-family:Arial;font-size:117%; color:aqua;display:none'><br> </span></div> </layer><script>
+ mytop = 0.09 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-134 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:aqua;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.79 * g_height; myleft = 0.83 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.83 * g_width;
+yy = 0.79 * g_height;
+ht = 0.11 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image078.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.84 * g_height; myleft = 0.83 * g_width; myheight = 0.02 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.83 * g_width;
+yy = 0.84 * g_height;
+ht = 0.02 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image079.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.79 * g_height; myleft = 0.53 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.53 * g_width;
+yy = 0.79 * g_height;
+ht = 0.11 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image080.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.83 * g_height; myleft = 0.53 * g_width; myheight = 0.01 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.53 * g_width;
+yy = 0.83 * g_height;
+ht = 0.01 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image081.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.8 * g_height; myleft = 0.23 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.23 * g_width;
+yy = 0.8 * g_height;
+ht = 0.11 * g_height;
+wd = 0.01 * g_width;
+document.write(
+'<img border=0 src="slide0019_image082.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.18 * g_height; myleft = 0.43 * g_width; myheight = 0.09 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The next few slides provide a short introduction to mapping the loci that are responsible for variation in a trait such as App expression level. These modulatory regions of the genome are sometimes called quantitative trait loci or QTLs. You may want to do some independent reading on this topic if this is your first exposure to QTL analysis.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The genetic reference population (GRP) of BXD recombinant inbred strains were originally generated about 25 years ago by Benjamin Taylor at The Jackson Laboratory. He crossed female C57BL/6J mice with male DBA/2J mice to generate the F1 and F2 progeny. At the bottom of this slide we have schematized one chromosome pair from three of the BXD RI strains.<span style="mso-spacerun: yes">&nbsp; </span>The dashed vertical lines that lead to the final BXD RI lines involve 21 full sib matings (about 7 years of breeding). Some lines die out during inbreeding. For example, there is no longer any BXD3 strain.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. Over the last decade, our group (Lu Lu and Rob Williams) and Jeremy Peirce and Lee Silver at Princeton have enlarged Ben TaylorÕs set. There are now just over 80 BXD strains. They have all been genotyped using about 13,700 markers (SNPs and microsatellites). These markers are used to define the ÒblueÓ and ÒredÓ regions of the chromosomes as shown in the figure above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. Chromosomes of RI GRPs usually have about 4 times as many recombinations as those of F2 animals. However, unlike an F2, both chromosomes of an RI are identical. Therefore, 50 RI strains contain as many recombinations as 100 F2 animals.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. BXD43 through BXD100 were generated using a special method that resulted in a further doubling of the average recombination density per chromosome. The entire set of 80 BXDs therefore contains as many recombinations as about 260 F2 animals.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-422 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:367%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.06 * g_height; myleft = 0.72 * g_width; myheight = 0.19 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:267%;color:white'>4 </span><span style='font-size: 233%;color:white'>units</span><span style='font-family:Palatino;font-size:333%; color:#E9EB5D;display:none'><br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.29 * g_height; myleft = 0.72 * g_width; myheight = 0.19 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.02 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:267%;color:white'>2</span><span style='font-size: 233%;color:white'> units</span><span style='font-family:Palatino;font-size: 333%;color:#E9EB5D;display:none'><br> </span></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-384 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.15 * g_height; myleft = 0.31 * g_width; myheight = 0.05 * g_height; mywidth = 0.4 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.31 * g_width;
+yy = 0.15 * g_height;
+ht = 0.05 * g_height;
+wd = 0.4 * g_width;
+document.write(
+'<img border=0 src="slide0020_image094.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.17 * g_height; myleft = 0.09 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.17 * g_height; myleft = 0.17 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.79 * g_height; myleft = 0.05 * g_width; myheight = 0.13 * g_height; mywidth = 1.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.12 * g_height; mywidth = 1.02 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:"-256 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;mso-line-spacing:"-256 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.02 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E2EBF0'>D</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%; color:#E9EB5D'>and </span><span style='font-size:233%;color:#F6BF69'>B</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%; color:#E9EB5D'>may be SNP-like variants in the promoter </span></span></layer><script>
+ mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 1.02 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:200%;color:#E9EB5D'>itself (cis QTL) or in </span><span style='font-size:200%;color:#EAEC5E'>upstream genes (trans QTLs)</span><span style='font-size:300%;color:#E9EB5D'>.</span></span></layer></div> </layer></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0.04 * g_width; myheight = 0.16 * g_height; mywidth = 0.22 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.22 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D'>UPSTREAM<br> </span></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.22 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D'>modulators<br> </span></div> </layer><script>
+ mytop = 0.1 * g_height; myleft = 0.01 * g_width; myheight = 0.05 * g_height; mywidth = 0.2 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center;mso-line-spacing:"-233 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D; mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.07 * g_height; myleft = 0.44 * g_width; myheight = 0.17 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:300%;color:#E2EBF0'>High<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.63 * g_height; myleft = 0.08 * g_width; myheight = 0.17 * g_height; mywidth = 0.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.63 * g_height; myleft = 0.15 * g_width; myheight = 0.17 * g_height; mywidth = 0.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> </layer><script>
+ mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-350 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.71 * g_height; myleft = 0.07 * g_width; myheight = 0.12 * g_height; mywidth = 0.16 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.14 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:200%;color:#E9EB5D'><i>cis QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:200%;color:#E9EB5D;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
+ mytop = 0.57 * g_height; myleft = -0.01 * g_width; myheight = 0.07 * g_height; mywidth = 1.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = -0.01 * g_width;
+yy = 0.57 * g_height;
+ht = 0.07 * g_height;
+wd = 1.01 * g_width;
+document.write(
+'<img border=0 src="slide0020_image095.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0.44 * g_height; myleft = 0.47 * g_width; myheight = 0.12 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.09 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-272 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:233%;color:#F6BF69'>Low<br> </span></div> </layer><script>
+ mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.07 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-272 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:233%;color:#F6BF69;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
+ mytop = 0.58 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.04 * g_height; mywidth = 1.12 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:"-200 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;mso-line-spacing:"-200 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Gadget;font-size:167%; color:white'>&gt;&gt;&gt;&gt;PROMOTER--ATG-Exon1-Intron1-Exon2-Intron2 - etc-3'UTR &gt;&gt;&gt;&gt;&gt;</span></div> </layer></div> </layer><script>
+ mytop = 0.37 * g_height; myleft = 0.08 * g_width; myheight = 0.2 * g_height; mywidth = 0.13 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script language=JavaScript><!--
+xx = 0.08 * g_width;
+yy = 0.37 * g_height;
+ht = 0.2 * g_height;
+wd = 0.13 * g_width;
+document.write(
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+//-->
+</script></div> </layer><script>
+ mytop = 0.27 * g_height; myleft = 0.05 * g_width; myheight = 0.12 * g_height; mywidth = 0.2 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.19 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-line-spacing:"-233 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:200%;color:#E9EB5D'><i>trans QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>This slide is illustrates two major types of QTLs that modulate variability in transcript-relative steady state abundance.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>1. cis QTLs are defined as QTLs that are closely linked to the gene whose transcript is the measured trait. For example, a polymorphism in the promoter that affects binding of a transcription factor. However, cis QTLs can be far upstream or downstream polymorphisms in enhancers or may be in 3Õ UTR binding sites that affect message stability.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. trans QTLs map far enough away from the location of the gene that gives rise to the transcript that is being measured so that we can be fairly certain that the QTL is not in the gene itself. The most blatant type of trans QTL would be a polymorphism in a transcription factor. But in the majority of cases, the trans QTLs can be far removed in a mechanistic sense from the actual events modulating transcript abundance. That is why there are three overlapping arrows in the figure.<span style="mso-spacerun: yes">&nbsp; </span>The way in which an upstream polymorphism influences a downstream difference in mRNA abundance can be indirect. Effects can:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>a.<span style="mso-spacerun: yes">&nbsp; </span>cross tissue types (a polymorphic liver enzyme may affect CNS gene expression)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>b.<span style="mso-spacerun: yes">&nbsp; </span>cross time (the modulator is only expressed for one day during development but has permanent effects in adults)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>c.<span style="mso-spacerun: yes">&nbsp; </span>may be contingent on environmental factors (heat shock may trigger the expression of a polymorphic factor that affects mRNA abundance).</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
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+
+}
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0052.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0052.htm
new file mode 100755
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+'<img border=0 src="slide0021_image099.png" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
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+</script></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0.02 * g_width; myheight = 0.08 * g_height; mywidth = 0.93 * g_width;
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+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.06 * g_height; mywidth = 0.91 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-line-spacing:"-358 0 -1"'><span style='font-family:Verdana;font-size:64%'>Discovering upstream modulatory loci</span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Please bring the Trait Data and Analysis window to the front and look for the Interval Mapping button. Confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Mapping Scale is set to GENETIC</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Permutation test YES<span style="mso-spacerun: yes">&nbsp; </span>(2000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Bootstrap test YES (2000 is the default number)</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0053.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0053.htm
new file mode 100755
index 00000000..d729b467
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+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0053.htm
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+if ( ! top.PPTPRESENTATION ) {
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This is a major output type: a so-called full-genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromosomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans at almost 0 megabases). The blue labels along the bottom of the figure list a subset of the 3795 markers that were used in mapping.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The thick blue wavy line running across chromosomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of all markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The red line and the red axis to the far right provides an estimate of the effect that a QTL has on expression of App (this estimate of the so-called additive effect tends to be too high). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher trait values. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and those that have the D/D genotype at a specific marker. For example, on distal Chr 7 the red line peaks at a value of about 0.2. That means that this region of chromosome 2 is responsible for a 0.4 unit expression difference between B/B strains and the D/D strains.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides a method to evaluate whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But using the bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 3 and Chr 7 under the LRS peaks. That is somewhat reassuring. But notice that a substantial number of bootstrap are scattered around on other chromosomes. About 30% of the bootstrap resamples have a peak on Chr 7. That is pretty good, but does makes us realize that the sample we are working with is still quite small and fragile.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The horizontal dashed lines at 10.5 and 17.3 are the likelihood ratio statistic (LRS) values associated with the suggestive and significant genome-wide probabilities that were established by permutations of phenotypes across genotypes. We shuffle randomly 2000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 17.3. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 3 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations. Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. If you click on the body of the map, say on the blue line, then you will generate a view on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. If you click on a marker symbol, then you will generate a new Trait data and Analysis window with the genotypes loaded into the window just like any other trait. More on this in Section 3.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4. You can drag these maps off of the browser window and onto your desktop. They will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5. There is also an option at the bottom of the page to download a 2X higher resolution image of this plot for papers and presentations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>6. You can also download the results of the analysis in a text format</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+</script><span style='text-align:left'><span style='font-size:117%;color:#E9EB5D'><i>separation between New York and Boston in either travel hours or kilometers</i></span><span style='font-size:150%;color:#E9EB5D'><i>.</i></span><span style='font-size: 167%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></layer></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The map on the top has an X-axis scale based on frequency of recombinations events between markers (B to D transitions, see slide 19 for a color-coded example). These so-called genetic maps are scaled in centimorgan (recombinations per 100 gametes). In contrast, the physical map shown below the genetic map has an X-axis scale based on DNA length measured in nucleotides or base-pairs. Notice the large difference between the two maps in the size of Chr 19 (large on the genetic scale but small on the physical scale).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Also notice the large difference in the width of the chromosome 7 QTL peak. In mice, recombinations occur with higher frequency toward the telomeric side (righ sidet) of each chromosome. As a result, genetic maps are stretched out more toward the telomere relative to a physical map. The QTL on distal Chr 7 is therefore actually more precisely mapped than might appear looking at the genetic map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The physical scale is becoming more useful than the genetic scale primarily because many other data types can be easily superimposed on a physical map. You will see more examples in the next several slides.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+</script><script language=JavaScript><!--
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+
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+//-->
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0055.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0055.htm
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+</script> <meta name=Description content="Jul-18-05: Physical map for distal chromosome 7"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=Javascript src=script.js></script>
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+wd = g_width;
+document.write(
+'<img src="master03_background.png" height=' + ht + ' width=' + wd + '>' );
+//-->
+</script></div> </layer><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.08 * g_height; mywidth = 1.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'>Physical map for distal chromosome 7</span><span style='font-family:Verdana;font-size: 73%;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.04 * g_height; mywidth = 0.92 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><span style='font-size:167%;color:#E9EB5D'>Distal Chr 7 from ~120 and 132 Mb may modulate</span><span style='font-size:167%;color:#E9EB5D'><i> App</i></span><span style='font-size:200%;color:#E9EB5D;mso-special-format: lastCR;display:none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Physical map of variation in App expression in brain on distal Chr 7 (a blow up of the whole-genome map on the previous slide).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Verdana size=3> You can now see that the X-axis is on a physical scale of megabases (Mb). The QTL peak is roughly between 120 and 132 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. The small irregular colored blocks and marks toward the top of the map mark the locations of genes superimposed on the physical map. Neighboring genes are offset slightly in the vertical axis for display purpose. Note one region of very high gene density from about 120 to 123 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. The orange hash marks along the X-axis represent the number of single nucleotide polymorphisms that distinguish the two parental strains (C57BL/6J and DBA/2J) from each other. We call this the SNP seismograph track (see Glossary for more details). Regions with low numbers of SNP have closely matched sequences and are less likely to contain QTLs.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4. As before, the thin red line shows the additive effect size. By convention the positive values signify the D alleles are associated with higher expression of App in this region of Chr 7 than the B alleles. The maximum effect size is about +0.20 log2 expression units per D allele. The differences been the BB and DD genotypes (BB and DD because each strain has two alleles; one per chromosome) is therefore about 2^0.4 = 1.32; or a 32% increment in DD relative to BB at this locus.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5. If you scroll just under the Physical Map you will see text that reads ÒDISPLAY from XXX Mb TO YYY MbÉ..Ó<span style="mso-spacerun: yes">&nbsp; </span>These physical maps are zoomable, a feature we will exploit to evaluate candidate genes in this QTL interval.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0056.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0056.htm
new file mode 100755
index 00000000..eba3fc05
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+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0056.htm
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content="Jul-18-05: Evaluating candidate genes"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=Javascript src=script.js></script>
+<script language=JavaScript>
+function font_size() {
+ var min = window.innerWidth/20;
+ if ( min > window.innerHeight/20 )
+ min = window.innerHeight/20;
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+function DoOnLoad() {
+ LoadNavSld("slide0056.htm");
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+</script> </head> <body onresize="restore()" onload="DoOnLoad()" style='font-size:&{font_size()};' bgcolor="#000000"> <script>
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+ mytop = -0.01 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.93 * g_width;
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+</script></div> </layer><script>
+ mytop = 0.03 * g_height; myleft = 0.69 * g_width; myheight = 0.17 * g_height; mywidth = 0.33 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.16 * g_height; mywidth = 0.31 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.31 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center'><span style='font-size:200%;color:#E9EB5D'><i>Right position<br> </i></span></div> </layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.1 * g_height; mywidth = 0.31 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.31 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-size:200%;color:#E9EB5D'><i>and high </i></span></span></layer><script>
+ mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.31 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:center'><span style='font-size:200%;color:#E9EB5D'><i>correlation</i></span></span></layer></div> </layer></div> </layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.12 * g_height; mywidth = 0.21 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center'><span style='font-size:267%;color:#E9EB5D'><i><span style="mso-spacerun: yes">&nbsp;</span></i></span><span style='font-size:200%; color:#E9EB5D'><i>= better<br> </i></span></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:center'><span style='font-size:200%;color:#E9EB5D'><i>candidates</i></span></div> </layer></div> </layer></div> </layer></div> </LAYER>
+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Evaluating candidate genes (CHECKED BOXES) responsible for variability in APP expression:</b></font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>A large number of genes are usually in the QTL interval and are therefore POSITIONAL CANDIDATES, but they will differ greatly in their biological and bioinformatic plausibility. Assume that the QTL has been located between 119 and 131 Mb (12 Mb). There will typically be 12 to 15 genes per Mb, so we might need to evaluate several hundred positional candidates. In this particular case there are about 100 known genes in this interval. Eight of these are highlighted in the table above with check marks in the boxes to the left.<span style="mso-spacerun: yes">&nbsp; </span>We need to highlight and objectively score the biologically relevant subset of all 100 positional candidate genes. We could look through gene ontologies and expression levels to help us shorten the list. An alternate way available using WebQTL is to generate a list of those genes in this interval that have transcripts that co-vary in expression with App expression. That is what the table shows.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. To replicate this table go back to the Trait Data and Analysis Form. Choose to sort correlations by POSITION and select RETURN = 500. Then scroll down the list to Chr 7 and review the subset of positional candidates that share expression with App. You should see a list similar to that shown above. Gtf3c1 is a good biological candidate and has a high covariation in expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>2. Caveat:<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>Of course, the gene or genes that control App expression may not be in this list. A protein coding difference might be the ultimate cause of variation in App transcript level and the expression covariation might be close to zero. Our list may also simply be missing the right transcript since the microarray is not truly comprehensive. Furthermore, even if the list contains the QT gene, an expression difference may only have been expressed early in development or even in another tissue such as liver. While it is important to recognize these caveats, it is equally important to devise a rational way to rank candidates given existing data. Coexpression is one of several criteria used to evaluate positional candidates. We will see others in the next slide.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>3. We can also assess the likelihood that candidates contain functional polymorphism in promoters and enhancers that affect their expression simply by mapping the transcripts of all candidate genes to see if they Òmap backÓ to the location of gene itself. A transcript that maps to its own location is referred to as a cis QTL. We essentially ask: Which of the the transcripts listed in the Correlation Table above (from Gtf3c1 to Zranb1) has variation in expression that maps to Chr 7 at about 120 Mb?<span style="mso-spacerun: yes">&nbsp; </span>The logic of this search is that if a gene controls the level of its own expression it is also much more likely to generate other downstream effects. The Gtf3c1 transcript is a weak cis QTL with a local LRS maximum of about 7.0 (D alleles are high). That is just about sufficient to declare it to be a cis QTL. [No whole genome correction is required and a point-wise p-value of 0.05 is the appropriate test. A p-value of 0.05 is roughly equivalent to an LRS of 6.0 (LOD = 1.3).]</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Even higher blow-up of part of the Chr 7 physical map of variation in App expression in brain.<span style="mso-spacerun: yes">&nbsp; </span>The QTL region actually extends from about 119 to 129.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>1. As mentioned in the previous slide another important approach to ranking candidates is based on the number of sequence variants that distinguish the parental strains. If we were sure that the sequences of the gene, its promoter, and its enhancers were identical between the strains then we could discount--but not eliminate--that gene as a candidate. The Gtf3c1 candidate almost falls into this category: of 663 known SNPs in and around this gene, only four differ between C57BL/6J and DBA/2J. Gtf3c1 is essentially identical-by-descent in these strains and is a less likely candidate. In contrast, if the two alleles of the gene have dozens of functional variants in exons, promoters, enhancers, and splice sites, then it becomes a higher priority candidate.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>Of course it only takes a single critical sequence variant to generate downstream effects. The argument above is really about the prior probabilities. Where would you place your bets given the information at hand?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2.<span style="mso-spacerun: yes">&nbsp; </span>If you scroll down the INTERVAL ANALYST you will find that Ctbp2 is a particularly interesting candidate that contains lots of SNPs (n = 75 and a SNP density of 0.55 SNP/Kb). Ctbp2 is also closer to our QTL peak than was Gtf3c1. Not only does Ctbp2 contain lots of SNPs but it is also is associated with a powerful cis QTL with an LRS of 24.2 (divide by 4.61 to get the equivalent LOD score of 5.25).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3.<span style="mso-spacerun: yes">&nbsp; </span>At this high magnification, individual genes are distinct. They are color coded by their density of SNPs. Bright orange represents those genes that have a high SNP density (C57BL/6J versus DBA/2J), black represents genes with low SNP density. Roll the cursor over a gene block and its name will pop up, along with information on exon number.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>4.<span style="mso-spacerun: yes">&nbsp; </span>Beneath the physical map you will find an INTERVAL ANALYST table that lists information on known genes in the region on which you have zoomed the Physical Map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>5.<span style="mso-spacerun: yes">&nbsp; </span>As always: error-checking is important. Some genes may be missing from the Interval Analyst (recent additions or errors of omission). In this case the Zranb1 gene that is located just proximal to Ctbp2 is not listed in the INTERVAL ANALYST. Double-check the interval using the Genome Browser links (blue and beige horizontal bars) at the top of the PHYSICAL MAP.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0058.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0058.htm
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index 00000000..47e08c87
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+++ b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0058.htm
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+<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 11"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href="webqtl_demo2.ppt.ppt"> <script>
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+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 1.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'>Evaluating </span><span style='font-family:Verdana;font-size:64%'><i>Ctbp2</i></span><span style='font-family:Verdana;font-size:64%'> as a candidate QTL for </span><span style='font-family:Verdana;font-size:64%'><i>App</i></span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ mytop = 0.52 * g_height; myleft = 0.55 * g_width; myheight = 0.37 * g_height; mywidth = 0.35 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.33 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%;color:#E9EB5D'><i> the </i></span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%;color:#E9EB5D'><i> the App QTL </i></span></span></layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This slide illustrates one reason why Ctbp2 should be considered a high priority positional candidate gene that may modulate the expression level of App.<span style="mso-spacerun: yes">&nbsp; </span>Ctbp2 is a strong cis QTL in some brain regions (here the data are taken from the striatum).<span style="mso-spacerun: yes">&nbsp; </span>If Ctbp2 contains variants that modulate its own expression then these expression differencess may produce many downstream effects. Of course, we now want to know much more about the known biology of Ctbp2. What kind of gene is it? To begin to answer that question we can use a number of resources listed in the LINKS page.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Arial size=3>1.</font><font face=Verdana size=3> The App QTL is bimodal. Perhaps there are actually two causal factors in this region--one close to 123 Mb and the other close to 127 Mb.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. The precision of QTL mapping depends on several factors, including the effect size and interactions among QTLs modulating a trait, the number of genetic individuals that are studied, and the distribution of recombinations in the study population.<span style="mso-spacerun: yes">&nbsp; </span>In the case above, the QTL(s) are likely to be confined to the interval from 120 to 132 Mb. The bootstrap test (yellow bars shown in some of the previous slides) can be usual for estimating the consiistency of QTL peaks.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'>Evaluating </span><span style='font-family:Verdana;font-size:64%'><i>Ctbp2</i></span><span style='font-family:Verdana;font-size:64%'> using other resources</span><span style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display: none'><i><br> </i></span></div> </layer></div> </layer><script>
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+ <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Ctbp2 should also be considered a high priority biological candidate gene responsible for modulating App expression levels. The<span style="mso-spacerun: yes">&nbsp; </span>C-terminal binding protein 2 is a transcriptional co-repressor also known as Ribeye.</font><font face=Times-Roman size=3> The gene produces two transcripts encoding distinct proteins. The short form is a transcriptional repressor that binds a Pro-X-Asp-Leu-Ser peptide motif common to adenoviral oncoprotein E1a and a related motif in BKLF. This short form also interacts<span style="mso-spacerun: yes">&nbsp; </span>with several transcription factors including EVI1, ZFPM1, and<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>ZFHX1A (aka TCF8, deltaEF1). The longer isoform is a major component of specialized synapses in photoreceptors. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notes:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. To find out more about CTBP2 protein and the Ctbp2 gene, link to iHOP at http://www.pdg.cnb.uam.es/UniPub/iHOP/ and type in CTBP2</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Try Arrowsmith at http://arrowsmith.psych.uic.edu/cgi-test/arrowsmith_uic/pubsmith.cgi</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. Both APP and CTBP2 are involved in oxidoreducatase activity or Notch signalling. To estabilish this common gene ontology visit NCBI<span style="mso-spacerun: yes">&nbsp; </span>http://www.ncbi.nih.gov/entrez/query.fcgi?db=gene<span style="mso-spacerun: yes">&nbsp; </span>and enter each gene symbol.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. You can get intersting hints regarding Ctbp2 expression partners by examining the genetic correlations between Ctbp2 probe set 1422887_a_at and all other transcripts on the M430 Affymetrix array. Use the Striatum data set because we already know from previous work (the previous slide) that this gene is a cis QTL.<span style="mso-spacerun: yes">&nbsp; </span>You should be able to show that Ctbp2 and Notch3 have antagonistic expression patterns in striatum. The negative genetic correlation with E2f4 is even stronger. The transcript also has a high positive genetic correlation with Rdh14. Of particualr interest with respect to APP protein processing, Ctbp2 covaries positiviely with Bace2 (the transcript of the beta site APP-cleaving enzyme 2).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0060.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0060.htm
new file mode 100755
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.91 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:nobullet;text-align:left;font-family:"Gill Sans"; font-weight:normal;font-style:normal;text-decoration:none;text-shadow:none; text-effect:none;mso-fareast-hint:no;layout-flow:horizontal;color:#FBFDB8; font-size:213%;mso-text-raise:0%;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0;mso-line-spacing:"-220 0 -216";mso-margin-left-alt: 292;mso-text-indent-alt:0'><span style='mso-special-format:nobullet;display: none;color:#FBFDB8;font-family:"Monotype Sorts";font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'>Summary of Part 2:</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
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+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.84 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.95 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'><span style='mso-special-format:numbullet; position:absolute;left:-2.62%;font-family:Arial'>3.</span></span><span style='font-family:"Gill Sans";font-size:200%;color:#E9EB5D;mso-color-index: 3'><span style="mso-spacerun: yes">&nbsp;</span>Discussed interpreting features of QTL maps including the </span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>LRS function, the additive effect function, the bootstrap bars, </span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>and the permutation thresholds.<br> </span></span></layer></div> </layer><script>
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+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 1 * g_width;
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='mso-special-format:numbullet;font-family:Arial;text-align:left; font-family:Verdana;font-weight:normal;font-style:normal;text-decoration:none; text-shadow:none;text-effect:none;mso-fareast-hint:no;layout-flow:horizontal; color:black;mso-color-index:1;font-size:80%;mso-text-raise:0%;mso-margin-left-alt: 144;mso-margin-left-alt:144'><script>
+ mytop = 0 * g_height; myleft = 0.02 * g_width; myheight = 0.05 * g_height; mywidth = 0.94 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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+ mytop = 0.58 * g_height; myleft = 0.05 * g_width; myheight = 0.26 * g_height; mywidth = 1.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 1.06 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>What does a QTL signify? A good QTL is a claim that a particular </span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>chromosomal region contains a causal source of variation in the </span></span></layer><script>
+ mytop = 0.1 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>phenotype. The importance of this hypothesis depends on the </span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>quality and relevance of the phenotype and the statistical </span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:200%; color:#E9EB5D;mso-color-index:3'>strength of the QTL. As usual, test and be skeptical.</span></span></layer></div> </layer></div> </LAYER>
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+//-->
+</script><script language=JavaScript><!--
+function playList() {
+
+}
+//-->
+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0061.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0061.htm
new file mode 100755
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+</script></div> </layer><script>
+ mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.08 * g_height; mywidth = 1.01 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div><script>
+ mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.98 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:"Gill Sans";font-weight:normal; font-style:normal;text-decoration:none;text-shadow:none;text-effect:none; mso-fareast-hint:no;layout-flow:horizontal;color:#E9EB5D;font-size:293%; mso-text-raise:0%;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0;mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233; mso-text-indent-alt:0'><span style='font-family:Verdana;font-size:64%'>Test Questions</span><span style='font-family:Verdana;font-size:73%;mso-special-format: lastCR;display:none'><i><br> </i></span></div> </layer></div> </layer><script>
+ mytop = 0.11 * g_height; myleft = 0.04 * g_width; myheight = 0.06 * g_height; mywidth = 1.08 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><span style='font-size:233%;color:#E9EB5D'>1. Evaluate candidates for the Chr 3 </span><span style='font-size:233%;color:#E9EB5D'><i>App</i></span><span style='font-size:233%;color:#E9EB5D'> QTL.</span><span style='font-size:233%; color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>2. Do </span><span style='font-size:233%;color:#E9EB5D'><i>App</i></span><span style='font-size: 233%;color:#E9EB5D'> and </span><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2</i></span><span style='font-size:233%;color:#E9EB5D'> expression share any </span></span></layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>other QTLs beside that on Chr 7?</span><span style='font-size:233%;color:#E9EB5D; mso-special-format:lastCR;display:none'><i><br> </i></span></span></layer></div> </layer><script>
+ mytop = 0.45 * g_height; myleft = 0.04 * g_width; myheight = 0.12 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.02 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>3. Can you exploit literature mining tools to </span></span></layer><script>
+ mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.05 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>find strong relation between </span><span style='font-size:233%;color:#E9EB5D'><i>App</i></span><span style='font-size:233%;color:#E9EB5D'> and </span><span style='font-size:233%; color:#E9EB5D'><i>Ctbp2</i></span><span style='font-size:233%;color:#E9EB5D'>? </span><span style='font-size:233%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></layer></div> </layer><script>
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
+ mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.1 * g_width;
+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:233%;color:#E9EB5D'>4. Why might the cis QTL for </span><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2</i></span><span style='font-size:233%;color:#E9EB5D'> expression </span></span></layer><script>
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+</script> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0062.htm b/web/tutorial/ppt/html/webqtl_demo2.ppt_files/slide0062.htm
new file mode 100755
index 00000000..6b671799
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+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
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+<script language=JavaScript>
+function font_size() {
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+ document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
+</script><span style='text-align:left'><span style='font-size:82%'>rwilliam@nb.utmem.edu<br> </span></span></layer><script>
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+}
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+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Jul-18-05> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:80% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript>
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+}
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+<script language=JavaScript>
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+function DoOnLoad() {
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+ window.location.replace( "endshow.htm" );
+}
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+ self.location.reload();
+}
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+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
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+
+ if ( parent.IsFullScrMode() && parent.NavPopup && !parent.NavPopup.closed )
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+ self.focus();
+ parent.base.SldUpdated( "slide0075.htm" );
+ }
+ </script><script language=JavaScript><!--
+function DoOnLoad() {
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+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0013.gif border=0 usemap="#Slide0013.gif_map"></center> </body> </html> \ No newline at end of file
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+ window.location.replace( "endshow.htm" );
+}
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+function restore() {
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+ self.location.reload();
+}
+</script>
+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
+DoOnLoad();
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+ self.focus();
+ parent.base.SldUpdated( "slide0076.htm" );
+ }
+ </script><script language=JavaScript><!--
+function DoOnLoad() {
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+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0014.gif border=0 usemap="#Slide0014.gif_map"></center> </body> </html> \ No newline at end of file
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+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Jul-18-05> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:80% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript>
+ if ( parent.base.msie < 0 ) {
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+function restore() {
+ if ( parent.base.msie < 0 )
+ self.location.reload();
+}
+</script>
+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
+DoOnLoad();
+
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+ if ( parent.IsFullScrMode() && parent.NavPopup && !parent.NavPopup.closed )
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+ self.focus();
+ parent.base.SldUpdated( "slide0077.htm" );
+ }
+ </script><script language=JavaScript><!--
+function DoOnLoad() {
+ }//-->
+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0015.gif border=0 usemap="#Slide0015.gif_map"></center> </body> </html> \ No newline at end of file
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new file mode 100755
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+if ( ! top.PPTPRESENTATION ) {
+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Jul-18-05> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:80% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript>
+ if ( parent.base.msie < 0 ) {
+ document.captureEvents(Event.MOUSEDOWN);
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+ document.onmousedown = _KPH;
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+function restore() {
+ if ( parent.base.msie < 0 )
+ self.location.reload();
+}
+</script>
+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
+DoOnLoad();
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+
+ if ( parent.IsFullScrMode() && parent.NavPopup && !parent.NavPopup.closed )
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+ else
+ self.focus();
+ parent.base.SldUpdated( "slide0078.htm" );
+ }
+ </script><script language=JavaScript><!--
+function DoOnLoad() {
+ }//-->
+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0016.gif border=0 usemap="#Slide0016.gif_map"></center> </body> </html> \ No newline at end of file
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+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Jul-18-05> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:80% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript>
+ if ( parent.base.msie < 0 ) {
+ document.captureEvents(Event.MOUSEDOWN);
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+ document.onmousedown = _KPH;
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+function restore() {
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+ self.location.reload();
+}
+</script>
+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
+DoOnLoad();
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+
+ if ( parent.IsFullScrMode() && parent.NavPopup && !parent.NavPopup.closed )
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+ self.focus();
+ parent.base.SldUpdated( "slide0079.htm" );
+ }
+ </script><script language=JavaScript><!--
+function DoOnLoad() {
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+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0017.gif border=0 usemap="#Slide0017.gif_map"></center> </body> </html> \ No newline at end of file
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new file mode 100755
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+ window.location.replace( "endshow.htm" );
+}
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+ if ( parent.base.msie < 0 ) {
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+function restore() {
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+ self.location.reload();
+}
+</script>
+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
+DoOnLoad();
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+ if ( parent.IsFullScrMode() && parent.NavPopup && !parent.NavPopup.closed )
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+ self.focus();
+ parent.base.SldUpdated( "slide0080.htm" );
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+ </script><script language=JavaScript><!--
+function DoOnLoad() {
+ }//-->
+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0018.gif border=0 usemap="#Slide0018.gif_map"></center> </body> </html> \ No newline at end of file
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new file mode 100755
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+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Jul-18-05> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:80% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript>
+ if ( parent.base.msie < 0 ) {
+ document.captureEvents(Event.MOUSEDOWN);
+ document.captureEvents(Event.KEYPRESS);
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+ document.onmousedown = _KPH;
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+function restore() {
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+ self.location.reload();
+}
+</script>
+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
+DoOnLoad();
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+ if ( parent.IsFullScrMode() && parent.NavPopup && !parent.NavPopup.closed )
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+ self.focus();
+ parent.base.SldUpdated( "slide0081.htm" );
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+ </script><script language=JavaScript><!--
+function DoOnLoad() {
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+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0019.gif border=0 usemap="#Slide0019.gif_map"></center> </body> </html> \ No newline at end of file
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+ window.location.replace( "endshow.htm" );
+}
+</script> <meta name=Description content=Jul-18-05> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:80% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript>
+ if ( parent.base.msie < 0 ) {
+ document.captureEvents(Event.MOUSEDOWN);
+ document.captureEvents(Event.KEYPRESS);
+ document.onkeypress = _KPH;
+ document.onmousedown = _KPH;
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+function restore() {
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+ self.location.reload();
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+</script>
+<script language=JavaScript>
+function flatLoad() {
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+ parent.base.highlite();
+DoOnLoad();
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+ if ( parent.IsFullScrMode() && parent.NavPopup && !parent.NavPopup.closed )
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+ parent.base.SldUpdated( "slide0082.htm" );
+ }
+ </script><script language=JavaScript><!--
+function DoOnLoad() {
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+</script> </head> <body bgcolor="#000000" onresize="restore()" onload="flatLoad()" onclick="DocumentOnClick(event)" onkeypress="_KPH(event)"> <center><img src=Slide0020.gif border=0 usemap="#Slide0020.gif_map"></center> </body> </html> \ No newline at end of file
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