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path: root/qc_app/upload/rqtl2.py
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"""Module to handle uploading of R/qtl2 bundles."""
import sys
import json
from pathlib import Path
from datetime import date
from uuid import UUID, uuid4
from zipfile import ZipFile, is_zipfile

from redis import Redis
from MySQLdb.cursors import DictCursor
from flask import (
    flash,
    request,
    url_for,
    redirect,
    Blueprint,
    render_template,
    current_app as app)

from r_qtl import r_qtl2
from r_qtl.errors import InvalidFormat

from qc_app import jobs
from qc_app.files import save_file, fullpath
from qc_app.dbinsert import species as all_species
from qc_app.db_utils import with_db_connection, database_connection

from qc_app.db.tissues import all_tissues, tissue_by_id
from qc_app.db.platforms import platform_by_id, platforms_by_species
from qc_app.db.averaging import averaging_methods, averaging_method_by_id
from qc_app.db import (
    species_by_id,
    save_population,
    populations_by_species,
    population_by_species_and_id,)
from qc_app.db.datasets import (
    geno_dataset_by_id,
    geno_datasets_by_species_and_population,

    probeset_study_by_id,
    probeset_create_study,
    probeset_dataset_by_id,
    probeset_create_dataset,
    probeset_datasets_by_study,
    probeset_studies_by_species_and_population)

rqtl2 = Blueprint("rqtl2", __name__)

@rqtl2.route("/", methods=["GET", "POST"])
@rqtl2.route("/select-species", methods=["POST"])
def select_species():
    """Select the species."""
    if request.method == "GET":
        return render_template("rqtl2/index.html", species=with_db_connection(all_species))

    species_id = request.form.get("species_id")
    species = with_db_connection(
        lambda conn: species_by_id(conn, species_id))
    if bool(species):
        return redirect(url_for(
            "upload.rqtl2.select_population", species_id=species_id))
    flash("Invalid species or no species selected!", "alert-error alert-rqtl2")
    return redirect(url_for("upload.rqtl2.select_species"))

@rqtl2.route("/upload/species/<int:species_id>/select-population",
             methods=["GET", "POST"])
def select_population(species_id: int):
    """Select/Create the population to organise data under."""
    with database_connection(app.config["SQL_URI"]) as conn:
        species = species_by_id(conn, species_id)
        if not bool(species):
            flash("Invalid species selected!", "alert-error alert-rqtl2")
            return redirect(url_for("upload.rqtl2.select_species"))

        if request.method == "GET":
            return render_template(
                "rqtl2/select-population.html",
                species=species,
                populations=populations_by_species(conn, species_id))

        population = population_by_species_and_id(
            conn, species["SpeciesId"], request.form.get("inbredset_id"))
        if not bool(population):
            flash("Invalid Population!", "alert-error alert-rqtl2")
            return redirect(
                url_for("upload.rqtl2.select_population", pgsrc="error"),
                code=307)

        return redirect(url_for("upload.rqtl2.upload_rqtl2_bundle",
                                species_id=species["SpeciesId"],
                                population_id=population["InbredSetId"]))

@rqtl2.route("/upload/species/<int:species_id>/create-population",
             methods=["POST"])
def create_population(species_id: int):
    """Create a new population for the given species."""
    population_page = redirect(url_for("upload.rqtl2.select_population"))
    with database_connection(app.config["SQL_URI"]) as conn:
        species = species_by_id(conn, species_id)
        population_name = request.form.get("inbredset_name", "").strip()
        population_fullname = request.form.get("inbredset_fullname", "").strip()
        if not bool(species):
            flash("Invalid species!", "alert-error alert-rqtl2")
            return redirect(url_for("upload.rqtl2.select_species"))
        if not bool(population_name):
            flash("Invalid Population Name!", "alert-error alert-rqtl2")
            return population_page
        if not bool(population_fullname):
            flash("Invalid Population Full Name!", "alert-error alert-rqtl2")
            return population_page
        new_population = save_population(conn, {
            "SpeciesId": species["SpeciesId"],
            "Name": population_name,
            "InbredSetName": population_fullname,
            "FullName": population_fullname,
            "Family": request.form.get("inbredset_family") or None,
            "Description": request.form.get("description") or None
        })

    flash("Population created successfully.", "alert-success")
    return redirect(
        url_for("upload.rqtl2.upload_rqtl2_bundle",
                population_id=new_population["population_id"],
                pgsrc="create-population"),
        code=307)

class __RequestError__(Exception): #pylint: disable=[invalid-name]
    """Internal class to avoid pylint's `too-many-return-statements` error."""

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle"),
    methods=["GET", "POST"])
def upload_rqtl2_bundle(species_id: int, population_id: int):
    """Allow upload of R/qtl2 bundle."""
    this_page_with_errors = redirect(url_for("upload.rqtl2.upload_rqtl2_bundle",
                                             species_id=species_id,
                                             population_id=population_id,
                                             pgsrc="error"),
                                     code=307)

    with database_connection(app.config["SQL_URI"]) as conn:
        species = species_by_id(conn, species_id)
        population = population_by_species_and_id(
            conn, species["SpeciesId"], population_id)
        if not bool(species):
            flash("Invalid species!", "alert-error alert-rqtl2")
            return redirect(url_for("upload.rqtl2.select_species"))
        if not bool(population):
            flash("Invalid Population!", "alert-error alert-rqtl2")
            return redirect(
                url_for("upload.rqtl2.select_population", pgsrc="error"),
                code=307)
        if request.method == "GET" or (
                request.method == "POST"
                and bool(request.args.get("pgsrc"))):
            return render_template("rqtl2/upload-rqtl2-bundle-step-01.html",
                                   species=species,
                                   population=population)

        if not bool(request.files.get("rqtl2_bundle")):
            raise __RequestError__("No R/qtl2 zip bundle provided.")

        the_file = save_file(
            request.files["rqtl2_bundle"], Path(app.config["UPLOAD_FOLDER"]))
        if not bool(the_file):
            raise __RequestError__("Please provide a valid R/qtl2 zip bundle.")

        if not is_zipfile(str(the_file)):
            raise __RequestError__("Invalid file! Expected a zip file.")

        try:
            with ZipFile(str(the_file), "r") as zfile:
                r_qtl2.validate_bundle(zfile)
                return render_template(
                    "rqtl2/upload-rqtl2-bundle-step-02.html",
                    species=species,
                    population=population,
                    rqtl2_bundle_file=the_file.name)#type: ignore[union-attr]
        except (InvalidFormat, __RequestError__) as exc:
            flash("".join(exc.args), "alert-error alert-rqtl2")
            return this_page_with_errors

def check_errors(conn, *args, **kwargs):#pylint: disable=[too-many-return-statements]
    """Check for select errors in the forms and return a page to redirect to."""
    species_id = kwargs.get("species_id") or request.form.get("species_id")
    population_id = (kwargs.get("population_id")
                     or request.form.get("population_id"))
    species = species_by_id(conn, species_id)
    population = population_by_species_and_id(conn, species_id, population_id)

    if "species" in args and not bool(species):
        flash("Invalid species!", "alert-error alert-rqtl2")
        return redirect(url_for("upload.rqtl2.select_species"))

    if "population" in args and not bool(population):
        flash("Invalid Population!", "alert-error alert-rqtl2")
        return redirect(
            url_for("upload.rqtl2.select_population", pgsrc="error"),
            code=307)

    if ("rqtl2_bundle_file" in args
        and not bool(request.form.get("rqtl2_bundle_file"))):
        flash("There is no file to process.", "alert-error alert-rqtl2")
        return redirect(url_for("upload.rqtl2.upload_rqtl2_bundle",
                                species_id=species_id,
                                population_id=population_id,
                                pgsrc="error"),
                        code=307)

    if ("geno-dataset" in args and
        not bool(request.form.get("geno-dataset-id"))):
        flash("No genotype dataset was provided!", "alert-error alert-rqtl2")
        return redirect(url_for("upload.rqtl2.select_geno_dataset",
                                species_id=species_id,
                                population_id=population_id,
                                pgsrc="error"),
                        code=307)

    summary_page = redirect(url_for("upload.rqtl2.select_dataset_info",
                                    species_id=species_id,
                                    population_id=population_id,
                                    pgsrc="error"),
                            code=307)

    if ("probe-study-id" in args and
        not bool(request.form.get("probe-study-id"))):
        flash("No probeset study was selected!", "alert-error alert-rqtl2")
        return summary_page

    if ("probe-dataset-id" in args and
        not bool(request.form.get("probe-dataset-id"))):
        flash("No probeset dataset was selected!", "alert-error alert-rqtl2")
        return summary_page

    return None

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/select-geno-dataset"),
             methods=["POST"])
def select_geno_dataset(species_id: int, population_id: int):
    """Select from existing geno datasets."""
    with database_connection(app.config["SQL_URI"]) as conn:
        error = check_errors(
            conn, "species", "population", "rqtl2_bundle_file", "geno-dataset")
        if bool(error):
            return error

        geno_dset = geno_datasets_by_species_and_population(
            conn, species_id, population_id)
        if not bool(geno_dset):
            flash("No genotype dataset was provided!",
                  "alert-error alert-rqtl2")
            return redirect(url_for("upload.rqtl2.select_geno_dataset",
                                    species_id=species_id,
                                    population_id=population_id,
                                    pgsrc="error"),
                            code=307)

        flash("Genotype accepted", "alert-success alert-rqtl2")
        return redirect(url_for("upload.rqtl2.select_dataset_info",
                                species_id=species_id,
                                population_id=population_id,
                                pgsrc="upload.rqtl2.select_geno_dataset"),
                        code=307)

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/create-geno-dataset"),
             methods=["POST"])
def create_geno_dataset(species_id: int, population_id: int):
    """Create a new geno dataset."""
    with database_connection(app.config["SQL_URI"]) as conn:
        error = check_errors(conn, "species", "population", "rqtl2_bundle_file")
        if bool(error):
            return error

        sgeno_page = redirect(url_for("upload.rqtl2.select_geno_dataset",
                                      species_id=species_id,
                                      population_id=population_id,
                                      pgsrc="error"),
                              code=307)
        if not bool(request.form.get("dataset-name")):
            flash("You must provide the dataset name",
                  "alert-error alert-rqtl2")
            return sgeno_page
        if not bool(request.form.get("dataset-fullname")):
            flash("You must provide the dataset full name",
                  "alert-error alert-rqtl2")
            return sgeno_page
        if not bool(request.form.get("dataset-shortname")):
            flash("You must provide the dataset short name",
                  "alert-error alert-rqtl2")
            return sgeno_page
        public = 2 if request.form.get("dataset-public") == "on" else 0

        with conn.cursor(cursorclass=DictCursor) as cursor:
            new_dataset = {
                "name": request.form.get("dataset-name"),
                "fname": request.form.get("dataset-fullname"),
                "sname": request.form.get("dataset-shortname"),
                "today": date.today().isoformat(),
                "pub": public,
                "isetid": population_id
            }
            cursor.execute(
                "INSERT INTO GenoFreeze("
                "Name, FullName, ShortName, CreateTime, public, InbredSetId"
                ") "
                "VALUES("
                "%(name)s, %(fname)s, %(sname)s, %(today)s, %(pub)s, %(isetid)s"
                ")",
                new_dataset)
            flash("Created dataset successfully.", "alert-success")
            return render_template(
                "rqtl2/create-geno-dataset-success.html",
                species=species_by_id(conn, species_id),
                population=population_by_species_and_id(
                    conn, species_id, population_id),
                rqtl2_bundle_file=request.form["rqtl2_bundle_file"],
                geno_dataset={**new_dataset, "id": cursor.lastrowid})

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/select-probeset-study"),
             methods=["POST"])
def select_probeset_study(species_id: int, population_id: int):
    """Select or create a probeset study."""
    with database_connection(app.config["SQL_URI"]) as conn:
        error = check_errors(
            conn, "species", "population", "rqtl2_bundle_file", "geno-dataset",
            "probe-study-id")
        if bool(error):
            return error

        summary_page = redirect(url_for("upload.rqtl2.select_dataset_info",
                                        species_id=species_id,
                                        population_id=population_id),
                                code=307)
        if not bool(probeset_study_by_id(conn, int(request.form["probe-study-id"]))):
            flash("Invalid study selected!", "alert-error alert-rqtl2")
            return summary_page

        return summary_page

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/select-probeset-dataset"),
             methods=["POST"])
def select_probeset_dataset(species_id: int, population_id: int):
    """Select or create a probeset dataset."""
    with database_connection(app.config["SQL_URI"]) as conn:
        error = check_errors(
            conn, "species", "population", "rqtl2_bundle_file", "geno-dataset",
            "probe-study-id", "probe-dataset-id")
        if bool(error):
            return error

        summary_page = redirect(url_for("upload.rqtl2.select_dataset_info",
                                        species_id=species_id,
                                        population_id=population_id),
                                code=307)
        if not bool(probeset_study_by_id(conn, int(request.form["probe-study-id"]))):
            flash("Invalid study selected!", "alert-error alert-rqtl2")
            return summary_page

        return summary_page

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/create-probeset-study"),
             methods=["POST"])
def create_probeset_study(species_id: int, population_id: int):
    """Create a new probeset study."""
    with database_connection(app.config["SQL_URI"]) as conn:
        error = check_errors(
            conn, "species", "population", "rqtl2_bundle_file", "geno-dataset")
        if bool(error):
            return error

        form = request.form
        select_study_page = redirect(
            url_for("upload.rqtl2.select_probeset_study",
                    species_id=species_id,
                    population_id=population_id),
            code=307)

        if not (bool(form.get("platformid")) and
                bool(platform_by_id(conn, int(form["platformid"])))):
            flash("Invalid platform selected.", "alert-error alert-rqtl2")
            return select_study_page

        if not (bool(form.get("tissueid")) and
                bool(tissue_by_id(conn, int(form["tissueid"])))):
            flash("Invalid tissue selected.", "alert-error alert-rqtl2")
            return select_study_page

        study = probeset_create_study(
            conn, population_id, int(form["platformid"]), int(form["tissueid"]),
            form["studyname"], form.get("studyfullname") or "",
            form.get("studyshortname") or "")
        return render_template(
            "rqtl2/create-probe-study-success.html",
            species=species_by_id(conn, species_id),
            population=population_by_species_and_id(
                conn, species_id, population_id),
            rqtl2_bundle_file=request.form["rqtl2_bundle_file"],
            geno_dataset=geno_dataset_by_id(
                conn,
                int(request.form["geno-dataset-id"])),
            study=study)

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/create-probeset-dataset"),
             methods=["POST"])
def create_probeset_dataset(species_id: int, population_id: int):#pylint: disable=[too-many-return-statements]
    """Create a new probeset dataset."""
    with database_connection(app.config["SQL_URI"]) as conn:
        error = check_errors(
            conn, "species", "population", "rqtl2_bundle_file", "geno-dataset",
            "probe-study-id")
        if bool(error):
            return error

        form = request.form
        summary_page = redirect(url_for("upload.rqtl2.select_dataset_info",
                                        species_id=species_id,
                                        population_id=population_id),
                                code=307)
        if not bool(form.get("averageid")):
            flash("Averaging method not selected!", "alert-error alert-rqtl2")
            return summary_page
        if not bool(form.get("datasetname")):
            flash("Dataset name not provided!", "alert-error alert-rqtl2")
            return summary_page
        if not bool(form.get("datasetfullname")):
            flash("Dataset full name not provided!", "alert-error alert-rqtl2")
            return summary_page

        study = probeset_study_by_id(conn, int(form["probe-study-id"]))
        if not bool(study):
            flash("Invalid ProbeSet study provided!", "alert-error alert-rqtl2")
            return summary_page

        avgmethod = averaging_method_by_id(conn, int(form["averageid"]))
        if not bool(avgmethod):
            flash("Invalid averaging method provided!", "alert-error alert-rqtl2")
            return summary_page

        dset = probeset_create_dataset(conn,
                                       int(form["probe-study-id"]),
                                       int(form["averageid"]),
                                       form["datasetname"],
                                       form["datasetfullname"],
                                       form["datasetshortname"],
                                       form["datasetpublic"] == "on",
                                       form.get("datasetdatascale", "log2"))
        return render_template(
            "rqtl2/create-probe-dataset-success.html",
            species=species_by_id(conn, species_id),
            population=population_by_species_and_id(
                conn, species_id, population_id),
            rqtl2_bundle_file=request.form["rqtl2_bundle_file"],
            geno_dataset=geno_dataset_by_id(
                conn,
                int(request.form["geno-dataset-id"])),
            study=study,
            avgmethod=avgmethod,
            dataset=dset)

@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/dataset-info"),
             methods=["POST"])
def select_dataset_info(species_id: int, population_id: int):
    """
    If `geno` files exist in the R/qtl2 bundle, prompt user to provide the
    dataset the genotypes belong to.
    """
    form = request.form
    with database_connection(app.config["SQL_URI"]) as conn:
        error_page = check_errors(conn, "species", "population", "rqtl2_bundle_file")
        if bool(error_page):
            return error_page

        species = species_by_id(conn, species_id)
        population = population_by_species_and_id(
            conn, species_id, population_id)
        thefile = fullpath(form["rqtl2_bundle_file"])
        with ZipFile(str(thefile), "r") as zfile:
            cdata = r_qtl2.control_data(zfile)
            if "geno" in cdata and not bool(form.get("geno-dataset-id")):
                return render_template(
                    "rqtl2/select-geno-dataset.html",
                    species=species,
                    population=population,
                    rqtl2_bundle_file=thefile.name,
                    datasets=geno_datasets_by_species_and_population(
                        conn, species_id, population_id))
            geno_dataset = geno_dataset_by_id(conn, int(form["geno-dataset-id"]))

            if "pheno" in cdata and not bool(form.get("probe-study-id")):
                return render_template(
                    "rqtl2/select-probeset-study-id.html",
                    species=species,
                    population=population,
                    rqtl2_bundle_file=thefile.name,
                    geno_dataset=geno_dataset,
                    studies=probeset_studies_by_species_and_population(
                            conn, species_id, population_id),
                    platforms=platforms_by_species(conn, species_id),
                    tissues=all_tissues(conn))
            probeset_study = probeset_study_by_id(
                conn, int(form["probe-study-id"]))

            if "pheno" in cdata and not bool(form.get("probe-dataset-id")):
                return render_template(
                    "rqtl2/select-probeset-dataset.html",
                    species=species,
                    population=population,
                    rqtl2_bundle_file=thefile.name,
                    geno_dataset=geno_dataset,
                    probe_study=probeset_study,
                    datasets=probeset_datasets_by_study(
                        conn, int(form["probe-study-id"])),
                    avgmethods=averaging_methods(conn))
            probeset_study = probeset_study_by_id(
                conn, int(form["probe-study-id"]))
            probeset_dataset = probeset_dataset_by_id(
                conn, int(form["probe-dataset-id"]))

    return render_template("rqtl2/summary-info.html",
                           species=species,
                           population=population,
                           rqtl2_bundle_file=thefile.name,
                           geno_dataset=geno_dataset,
                           probe_study=probeset_study,
                           probe_dataset=probeset_dataset)



@rqtl2.route(("/upload/species/<int:species_id>/population/<int:population_id>"
              "/rqtl2-bundle/confirm-bundle-details"),
             methods=["POST"])
def confirm_bundle_details(species_id: int, population_id: int):
    """Confirm the details and trigger R/qtl2 bundle processing..."""
    redisuri = app.config["REDIS_URL"]
    with (database_connection(app.config["SQL_URI"]) as conn,
          Redis.from_url(redisuri, decode_responses=True) as rconn):
        error = check_errors(
            conn, "species", "population", "rqtl2_bundle_file", "geno-dataset",
            "probe-study-id", "probe-dataset-id")
        if bool(error):
            return error

        redis_ttl_seconds = app.config["JOBS_TTL_SECONDS"]
        jobid = str(uuid4())
        _job = jobs.launch_job(
            jobs.initialise_job(
                rconn,
                jobid,
                [
                    sys.executable, "-m", "scripts.process_rqtl2_bundle",
                    app.config["SQL_URI"], app.config["REDIS_URL"], jobid,
                    "--redisexpiry", str(redis_ttl_seconds)],
                "R/qtl2 Bundle Upload",
                redis_ttl_seconds,
                {
                    "bundle-metadata": json.dumps({
                        "speciesid": species_id,
                        "populationid": population_id,
                        "rqtl2-bundle-file": str(fullpath(
                            request.form["rqtl2_bundle_file"])),
                        "geno-dataset-id": request.form.get(
                            "geno-dataset-id", ""),
                        "probe-study-id": request.form.get(
                            "probe-study-id", ""),
                        "probe-dataset-id": request.form.get(
                            "probe-dataset-id", ""),
                        **({
                            "platformid": probeset_study_by_id(
                                conn,
                                int(request.form["probe-study-id"]))["ChipId"]
                        } if bool(request.form.get("probe-study-id")) else {})
                    })
                }),
            redisuri,
            f"{app.config['UPLOAD_FOLDER']}/job_errors")

        return redirect(url_for("upload.rqtl2.rqtl2_processing_status",
                                jobid=jobid))

@rqtl2.route("/status/<uuid:jobid>")
def rqtl2_processing_status(jobid: UUID):
    """Retrieve the status of the job processing the uploaded R/qtl2 bundle."""
    with Redis.from_url(app.config["REDIS_URL"], decode_responses=True) as rconn:
        thejob = jobs.job(rconn, jobid)
        if not bool(thejob):
            return render_template("rqtl2/no-such-job.html", jobid=jobid)

        messagelistname = thejob.get("log-messagelist")
        logmessages = (rconn.lrange(messagelistname, 0, -1)
                       if bool(messagelistname) else [])

        if thejob["status"] == "error":
            return render_template(
                "rqtl2/rqtl2-job-error.html", job=thejob, messages=logmessages)
        if thejob["status"] == "success":
            return render_template("rqtl2/rqtl2-job-results.html",
                                   job=thejob,
                                   messages=logmessages)

        return render_template(
            "rqtl2/rqtl2-job-status.html", job=thejob, messages=logmessages)