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<p>Brown adipose was later shattered in liquid nitrogen and about half was taken for each sample, although the size of the BAT varied dramatically. All ~5 animals per cohort had their RNA prepared, and then were pooled evenly (by µg of RNA) into a single RNA sample for each cohort. These pooled RNA samples were then purified using RNEasy, then sent out for array analysis. All RIN values were > 8.0.</p>
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