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diff --git a/general/datasets/Tigem_hg38_ret_rna_seq_0916/experiment-design.rtf b/general/datasets/Tigem_hg38_ret_rna_seq_0916/experiment-design.rtf new file mode 100644 index 0000000..f9da145 --- /dev/null +++ b/general/datasets/Tigem_hg38_ret_rna_seq_0916/experiment-design.rtf @@ -0,0 +1,3 @@ +<h3>RNA extraction, library preparation and sequencing</h3>
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+<p>Total RNA was extracted from the 50 human retina samples using the miRNeasy Kit (QIAGEN) according to the manufacturer's instructions. RNA was quantified using a NanoDrop ND-8000 spectrophotometer (NanoDrop Technologies) and the integrity was evaluated using an RNA 6000 Nano chip on a Bioanalyzer (Agilent Technologies). The RNA of the 50 samples had an average RNA integrity number (RIN) of 8.7 (ranging from 7.2 to 9.7). Libraries were prepared according to manufacturer's instructions (TruSeq RNA Sample Preparation kit) with an initial amount of 4 μg of total RNA. Quality control of library templates was performed using a High Sensitivity DNA Assay kit (Agilent Technologies) on a Bioanalyzer (Agilent Technologies). Qubit quantification platform was used to normalize samples for the library preparation (Qubit 2.0 Fluorometer, Life Technologies). Libraries were sequenced via a paired-end chemistry on an Illumina HiSeq1000 platform with an average yield of ∼6 Mb.</p>
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