diff options
Diffstat (limited to 'general/datasets/HC_U_0304_R/experiment-design.rtf')
| -rw-r--r-- | general/datasets/HC_U_0304_R/experiment-design.rtf | 7 |
1 files changed, 7 insertions, 0 deletions
diff --git a/general/datasets/HC_U_0304_R/experiment-design.rtf b/general/datasets/HC_U_0304_R/experiment-design.rtf new file mode 100644 index 0000000..5b0ad70 --- /dev/null +++ b/general/datasets/HC_U_0304_R/experiment-design.rtf @@ -0,0 +1,7 @@ +<p>About amplification and hybridization:</p>
+
+<p>Total RNA was quantified using RiboGreen and split into equal aliquots of approximately 10 ng, representing RNA from approximately 10,000 cells, and labeled using a total of three rounds of RNA amplification, exactly as described previously (Scherer et al. <a class="normal" href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12661160&dopt=Abstract" target="_blank">2003</a>). Labeled cRNA was fractionated and hybridized to the U74Av2 microarray following standard Affymetrix protocols.</p>
+
+<p>About the chromosome and megabase position values:</p>
+
+<blockquote>The chromosomal locations of probe sets and gene markers were determined by BLAT analysis using the Mouse Genome Sequencing Consortium Oct 2003 Assembly (see <a class="normal" href="http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&org=mouse">http://genome.ucsc.edu/</a>). We thank Yan Cui (UTHSC) for allowing us to use his Linux cluster to perform this analysis.</blockquote>
|