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authorBonface2024-02-13 23:52:26 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commitb2feda451ccfbeaed02dce9088d6dd228cf15861 (patch)
tree3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/Lvf2_m_0704_r/cases.rtf
parentd029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff)
downloadgn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz
Update dataset RTF Files.
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+<blockquote>The F<sub>2</sub>-ob/ob mice were chosen from a mapping panel that we created to map diabetes related physiological phenotypes (Stoehr et al. 2000). About 110 of these F<sub>2</sub>-ob/ob mice were also used to map mRNA abundance traits derived by quantitative real-time RT-PCR (Lan et al. 2003). The sixty F<sub>2</sub>-ob/ob mice that were used to generate microarray-derived mRNA abundance traits were selected from the 110 mice based on a selective phenotyping algorithm (Jin et al. 2004). The F<sub>2</sub>-ob/ob mice were housed at weaning at the University of Wisconsin-Madison animal care facility on a 12-h light/dark cycle. Mice were provided Purina Formulab Chow 5008 (6.5% fat) and acidified water ad libitum. Mice were killed at 14 weeks of age by CO<sub>2</sub> asphyxiation after a 4-hour fast. The livers, along with other tissues, were immediately foil wrapped and frozen in liquid nitrogen, and subsequently transferred to -80 &deg;C freezers for storage.</blockquote>