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authorBonface2024-02-15 06:09:54 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commite34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534 (patch)
tree67c6bdeb413af7d1dd6c4d02f37b206850a78531 /general/datasets/KIN_YSM_AMY_0711/experiment-design.rtf
parentb2feda451ccfbeaed02dce9088d6dd228cf15861 (diff)
downloadgn-docs-e34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534.tar.gz
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-<p>Human Brain Specimens and Tissue Processing This study was carried out using post-mortem human brain specimens collected from the Human Fetal Tissue Repository at the Albert Einstein College of Medicine (AECOM). Dissected tissue was fresh-frozen in Trizol for RNA and DNA extraction, with a post-mortem interval of less than 1 hour. Remaining tissue was fixed and frozen, and sections were analyzed for neuropathological or developmental defects. Details of specimens, tissue processing, microdissection, and neuropathological assessment are given in the Supplemental Experimental Procedures and Table S1. These studies were approved by the Human Investigation Committees of AECOM and Yale University.</p>
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-<p>For initial analysis, all neocortex samples were grouped together. In subsequent analyses, neocortex areas were compared with each other. In most analyses reported, left and right side samples were treated as additional biological replicates.</p>