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| author | Bonface | 2024-02-13 23:52:26 -0600 |
|---|---|---|
| committer | Munyoki Kilyungi | 2024-08-09 13:30:43 +0300 |
| commit | b2feda451ccfbeaed02dce9088d6dd228cf15861 (patch) | |
| tree | 3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/Hzi_ltcf_0313/processing.rtf | |
| parent | d029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff) | |
| download | gn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz | |
Update dataset RTF Files.
Diffstat (limited to 'general/datasets/Hzi_ltcf_0313/processing.rtf')
| -rw-r--r-- | general/datasets/Hzi_ltcf_0313/processing.rtf | 1 |
1 files changed, 1 insertions, 0 deletions
diff --git a/general/datasets/Hzi_ltcf_0313/processing.rtf b/general/datasets/Hzi_ltcf_0313/processing.rtf new file mode 100644 index 0000000..5502082 --- /dev/null +++ b/general/datasets/Hzi_ltcf_0313/processing.rtf @@ -0,0 +1 @@ +<p>Read Mapping and Gene Expression Quantification RNA-seq reads were quality-trimmed using Trim Galore (31) and mapped to the mm10 reference genome or to the IAV PR8M genome using STAR (32). Counts were summarized at the gene level using the R-package Rsubread (33), normalized and log transformed using the R-package DESeq2 (34), and batchcorrected using the ComBat function of the R-package sva (35, 36). For annotations of genes, ENTREZID from Rsubread were matched to RefSeq annotations using R-package biomart (37).</p>
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