diff options
| author | Bonface | 2024-02-13 23:52:26 -0600 |
|---|---|---|
| committer | Munyoki Kilyungi | 2024-08-09 13:30:43 +0300 |
| commit | b2feda451ccfbeaed02dce9088d6dd228cf15861 (patch) | |
| tree | 3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/Hc_u_0303_m/experiment-design.rtf | |
| parent | d029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff) | |
| download | gn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz | |
Update dataset RTF Files.
Diffstat (limited to 'general/datasets/Hc_u_0303_m/experiment-design.rtf')
| -rw-r--r-- | general/datasets/Hc_u_0303_m/experiment-design.rtf | 7 |
1 files changed, 7 insertions, 0 deletions
diff --git a/general/datasets/Hc_u_0303_m/experiment-design.rtf b/general/datasets/Hc_u_0303_m/experiment-design.rtf new file mode 100644 index 0000000..5b0ad70 --- /dev/null +++ b/general/datasets/Hc_u_0303_m/experiment-design.rtf @@ -0,0 +1,7 @@ +<p>About amplification and hybridization:</p>
+
+<p>Total RNA was quantified using RiboGreen and split into equal aliquots of approximately 10 ng, representing RNA from approximately 10,000 cells, and labeled using a total of three rounds of RNA amplification, exactly as described previously (Scherer et al. <a class="normal" href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12661160&dopt=Abstract" target="_blank">2003</a>). Labeled cRNA was fractionated and hybridized to the U74Av2 microarray following standard Affymetrix protocols.</p>
+
+<p>About the chromosome and megabase position values:</p>
+
+<blockquote>The chromosomal locations of probe sets and gene markers were determined by BLAT analysis using the Mouse Genome Sequencing Consortium Oct 2003 Assembly (see <a class="normal" href="http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&org=mouse">http://genome.ucsc.edu/</a>). We thank Yan Cui (UTHSC) for allowing us to use his Linux cluster to perform this analysis.</blockquote>
|