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| author | Bonface | 2024-02-09 09:41:28 -0600 |
|---|---|---|
| committer | Munyoki Kilyungi | 2024-08-09 13:30:43 +0300 |
| commit | d029d5d7f8ead1f1de8d318045004a4a6f68f5fb (patch) | |
| tree | 33c7ff40e3f953d030ed08f468f7afb1dfcba9e6 /general/datasets/HZI_LungBXD_RNA_Seq_1116/processing.rtf | |
| parent | 769ff7825f5d8d36d541e90534c07f1985899973 (diff) | |
| download | gn-docs-d029d5d7f8ead1f1de8d318045004a4a6f68f5fb.tar.gz | |
Update dataset RTF Files.
Diffstat (limited to 'general/datasets/HZI_LungBXD_RNA_Seq_1116/processing.rtf')
| -rw-r--r-- | general/datasets/HZI_LungBXD_RNA_Seq_1116/processing.rtf | 1 |
1 files changed, 1 insertions, 0 deletions
diff --git a/general/datasets/HZI_LungBXD_RNA_Seq_1116/processing.rtf b/general/datasets/HZI_LungBXD_RNA_Seq_1116/processing.rtf new file mode 100644 index 0000000..5502082 --- /dev/null +++ b/general/datasets/HZI_LungBXD_RNA_Seq_1116/processing.rtf @@ -0,0 +1 @@ +<p>Read Mapping and Gene Expression Quantification RNA-seq reads were quality-trimmed using Trim Galore (31) and mapped to the mm10 reference genome or to the IAV PR8M genome using STAR (32). Counts were summarized at the gene level using the R-package Rsubread (33), normalized and log transformed using the R-package DESeq2 (34), and batchcorrected using the ComBat function of the R-package sva (35, 36). For annotations of genes, ENTREZID from Rsubread were matched to RefSeq annotations using R-package biomart (37).</p>
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