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authorBonface2024-02-09 09:41:28 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commitd029d5d7f8ead1f1de8d318045004a4a6f68f5fb (patch)
tree33c7ff40e3f953d030ed08f468f7afb1dfcba9e6 /general/datasets/HZI_LTCF_0313/processing.rtf
parent769ff7825f5d8d36d541e90534c07f1985899973 (diff)
downloadgn-docs-d029d5d7f8ead1f1de8d318045004a4a6f68f5fb.tar.gz
Update dataset RTF Files.
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+<p>Read Mapping and Gene Expression Quantification RNA-seq reads were quality-trimmed using Trim Galore (31) and mapped to the mm10 reference genome or to the IAV PR8M genome using STAR (32). Counts were summarized at the gene level using the R-package Rsubread (33), normalized and log transformed using the R-package DESeq2 (34), and batchcorrected using the ComBat function of the R-package sva (35, 36). For annotations of genes, ENTREZID from Rsubread were matched to RefSeq annotations using R-package biomart (37).</p>