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| author | Bonface | 2024-02-13 23:52:26 -0600 |
|---|---|---|
| committer | Munyoki Kilyungi | 2024-08-09 13:30:43 +0300 |
| commit | b2feda451ccfbeaed02dce9088d6dd228cf15861 (patch) | |
| tree | 3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/HZI_LTCF_0313/cases.rtf | |
| parent | d029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff) | |
| download | gn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz | |
Update dataset RTF Files.
Diffstat (limited to 'general/datasets/HZI_LTCF_0313/cases.rtf')
| -rw-r--r-- | general/datasets/HZI_LTCF_0313/cases.rtf | 1 |
1 files changed, 0 insertions, 1 deletions
diff --git a/general/datasets/HZI_LTCF_0313/cases.rtf b/general/datasets/HZI_LTCF_0313/cases.rtf deleted file mode 100644 index 50868a3..0000000 --- a/general/datasets/HZI_LTCF_0313/cases.rtf +++ /dev/null @@ -1 +0,0 @@ -<p>Mice We used females from 41 BXD RI strains and both parental strains—B6 and D2. Mice were between 8 and 12 weeks of age when infected. They were housed and maintained on a 12:12 light/dark cycle, with ad libitum access to food and water. Virus Original stocks of mouse-adapted A/Puerto Rico/8/34 (H1N1, PR8M) virus were obtained from Stefan Ludwig, University of Münster (28). Virus stocks were propagated in the chorioallantoic cavity of 10-day-old pathogen-free embryonated chicken eggs for 48 h at 37°C as described previously (29). Viral titer was determined using a focus-forming unit (FFU) assay as described previously (29). Infection of Mice Animals were anesthetized by intraperitoneal injection of ketamine/xylazine (10 % (v/v) of 100 mg/ml ketamine and 5 % (v/v) of 20 mg/ml xylazine in 0.9 % (w/v) NaCl with a dose adjusted to body weight (200 μl/20 g body weight). Infection was performed by intranasal application of virus solution in 20 μl sterile phosphate-buffered saline (PBS), with a PR8M dosage of 2×103 FFU. Mice were bred and infected at the animal facilities at UTHSC</p>
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