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| author | Bonface | 2024-02-09 09:41:28 -0600 |
|---|---|---|
| committer | Munyoki Kilyungi | 2024-08-09 13:30:43 +0300 |
| commit | d029d5d7f8ead1f1de8d318045004a4a6f68f5fb (patch) | |
| tree | 33c7ff40e3f953d030ed08f468f7afb1dfcba9e6 /general/datasets/HC_U_0903_M/experiment-design.rtf | |
| parent | 769ff7825f5d8d36d541e90534c07f1985899973 (diff) | |
| download | gn-docs-d029d5d7f8ead1f1de8d318045004a4a6f68f5fb.tar.gz | |
Update dataset RTF Files.
Diffstat (limited to 'general/datasets/HC_U_0903_M/experiment-design.rtf')
| -rw-r--r-- | general/datasets/HC_U_0903_M/experiment-design.rtf | 7 |
1 files changed, 7 insertions, 0 deletions
diff --git a/general/datasets/HC_U_0903_M/experiment-design.rtf b/general/datasets/HC_U_0903_M/experiment-design.rtf new file mode 100644 index 0000000..5b0ad70 --- /dev/null +++ b/general/datasets/HC_U_0903_M/experiment-design.rtf @@ -0,0 +1,7 @@ +<p>About amplification and hybridization:</p>
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+<p>Total RNA was quantified using RiboGreen and split into equal aliquots of approximately 10 ng, representing RNA from approximately 10,000 cells, and labeled using a total of three rounds of RNA amplification, exactly as described previously (Scherer et al. <a class="normal" href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12661160&dopt=Abstract" target="_blank">2003</a>). Labeled cRNA was fractionated and hybridized to the U74Av2 microarray following standard Affymetrix protocols.</p>
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+<p>About the chromosome and megabase position values:</p>
+
+<blockquote>The chromosomal locations of probe sets and gene markers were determined by BLAT analysis using the Mouse Genome Sequencing Consortium Oct 2003 Assembly (see <a class="normal" href="http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&org=mouse">http://genome.ucsc.edu/</a>). We thank Yan Cui (UTHSC) for allowing us to use his Linux cluster to perform this analysis.</blockquote>
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