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authorBonface2024-02-15 06:09:54 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commite34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534 (patch)
tree67c6bdeb413af7d1dd6c4d02f37b206850a78531 /general/datasets/HC_U_0303_M/experiment-design.rtf
parentb2feda451ccfbeaed02dce9088d6dd228cf15861 (diff)
downloadgn-docs-e34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534.tar.gz
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-<p>About amplification and hybridization:</p>
-
-<p>Total RNA was quantified using RiboGreen and split into equal aliquots of approximately 10 ng, representing RNA from approximately 10,000 cells, and labeled using a total of three rounds of RNA amplification, exactly as described previously (Scherer et al. <a class="normal" href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=12661160&amp;dopt=Abstract" target="_blank">2003</a>). Labeled cRNA was fractionated and hybridized to the U74Av2 microarray following standard Affymetrix protocols.</p>
-
-<p>About the chromosome and megabase position values:</p>
-
-<blockquote>The chromosomal locations of probe sets and gene markers were determined by BLAT analysis using the Mouse Genome Sequencing Consortium Oct 2003 Assembly (see <a class="normal" href="http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&amp;org=mouse">http://genome.ucsc.edu/</a>). We thank Yan Cui (UTHSC) for allowing us to use his Linux cluster to perform this analysis.</blockquote>