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author | Bonface | 2024-02-13 23:52:26 -0600 |
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committer | Munyoki Kilyungi | 2024-08-09 13:30:43 +0300 |
commit | b2feda451ccfbeaed02dce9088d6dd228cf15861 (patch) | |
tree | 3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/Gse15222_f_n_ri_0409/platform.rtf | |
parent | d029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff) | |
download | gn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz |
Update dataset RTF Files.
Diffstat (limited to 'general/datasets/Gse15222_f_n_ri_0409/platform.rtf')
-rw-r--r-- | general/datasets/Gse15222_f_n_ri_0409/platform.rtf | 5 |
1 files changed, 5 insertions, 0 deletions
diff --git a/general/datasets/Gse15222_f_n_ri_0409/platform.rtf b/general/datasets/Gse15222_f_n_ri_0409/platform.rtf new file mode 100644 index 0000000..5bcda8b --- /dev/null +++ b/general/datasets/Gse15222_f_n_ri_0409/platform.rtf @@ -0,0 +1,5 @@ +<p>Illumina Human 50 mer probes. Total of 24357 probes according to Myers et al. A total of 24354 probes included in this GeneNetwork file.</p>
+
+<p>From the Methods section of the paper:</p>
+
+<p>Genotyping and Expression Proï¬ling DNA was hybridized to the Affymetrix GeneChip Human Mapping 500K Array Set (502,627 SNPs) as previously described.11,12 Genotypes were extracted with the use of both SNiPer-HD13 and BRLMM (Affymetrix, Santa Clara, CA) algorithms. Genotypes that exhibited less than 98% concordance between calls were excluded. SNPs with call rates less than 90% were excluded from the analysis. HardyWeinberg equilibrium (HWE) was assessed with exact tests and the PLINK analysis toolset.14 SNPs with HWE exact-test p values less than 0.05, as well as SNPs with minor-allele frequencies less than 1%, were excluded. Allele calls had a mean of 97% and a range of 90%–99%. cRNA was hybridized to Illumina Human Refseq-8 Expression BeadChip (24,357 transcripts) via standard protocols. Expression proï¬les were extracted and rank invariant normalized15–17 with the use of the BeadStudio software available from Illumina, with the Illumina custom error model used. Rankinvariant-normalized expression data were log10 transformed, and missing data were encoded as missing, rather than as a zero level of expression.</p>
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