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authorBonface2024-02-15 06:09:54 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commite34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534 (patch)
tree67c6bdeb413af7d1dd6c4d02f37b206850a78531 /general/datasets/GSE15222_F_N_RI_0409/platform.rtf
parentb2feda451ccfbeaed02dce9088d6dd228cf15861 (diff)
downloadgn-docs-e34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534.tar.gz
Update dataset RTF Files.
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-<p>Illumina Human 50 mer probes. Total of 24357 probes according to Myers et al. A total of 24354 probes included in this GeneNetwork file.</p>
-
-<p>From the Methods section of the paper:</p>
-
-<p>Genotyping and Expression Profiling DNA was hybridized to the Affymetrix GeneChip Human Mapping 500K Array Set (502,627 SNPs) as previously described.11,12 Genotypes were extracted with the use of both SNiPer-HD13 and BRLMM (Affymetrix, Santa Clara, CA) algorithms. Genotypes that exhibited less than 98% concordance between calls were excluded. SNPs with call rates less than 90% were excluded from the analysis. HardyWeinberg equilibrium (HWE) was assessed with exact tests and the PLINK analysis toolset.14 SNPs with HWE exact-test p values less than 0.05, as well as SNPs with minor-allele frequencies less than 1%, were excluded. Allele calls had a mean of 97% and a range of 90%&ndash;99%. cRNA was hybridized to Illumina Human Refseq-8 Expression BeadChip (24,357 transcripts) via standard protocols. Expression profiles were extracted and rank invariant normalized15&ndash;17 with the use of the BeadStudio software available from Illumina, with the Illumina custom error model used. Rankinvariant-normalized expression data were log10 transformed, and missing data were encoded as missing, rather than as a zero level of expression.</p>