diff options
author | Bonface | 2024-02-13 23:52:26 -0600 |
---|---|---|
committer | Munyoki Kilyungi | 2024-08-09 13:30:43 +0300 |
commit | b2feda451ccfbeaed02dce9088d6dd228cf15861 (patch) | |
tree | 3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/Eye_m2_1105_r/tissue.rtf | |
parent | d029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff) | |
download | gn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz |
Update dataset RTF Files.
Diffstat (limited to 'general/datasets/Eye_m2_1105_r/tissue.rtf')
-rw-r--r-- | general/datasets/Eye_m2_1105_r/tissue.rtf | 1554 |
1 files changed, 1554 insertions, 0 deletions
diff --git a/general/datasets/Eye_m2_1105_r/tissue.rtf b/general/datasets/Eye_m2_1105_r/tissue.rtf new file mode 100644 index 0000000..ec39390 --- /dev/null +++ b/general/datasets/Eye_m2_1105_r/tissue.rtf @@ -0,0 +1,1554 @@ +<blockquote>
+<p><strong>Tissue preparation protocol</strong>. Animal were killed by rapid cervical dislocation. Eyes were removed immediately and placed in RNA<em>later</em> at room temperature. Usually six eyes from animals with a common sex, age, and strain were stored in a single tube.</p>
+
+<p>Each array was hybridized with a pool of RNA from 4 to 8 eyes from 2 to 4 animals. RNA was extracted at UTHSC by Zhiping Jia. If tissue was saved for RNA extraction at a later time, eyes were placed directly in RNA<em>later</em> (Ambion, Inc.) and treated per the manufacturer’s directions. If eyes were used for immediate RNA extraction then we proceeded immediately to the next steps.</p>
+
+<p>Dissecting and preparing eyes for RNA extraction</p>
+
+<ol>
+ <li>Place eyes for RNA extraction in RNA STAT-60 (Tel-Test Inc.) and process per manufacturer’s instructions (in brief form below).</li>
+ <li>Store RNA in 75% ethanol at –80 deg. C until use.</li>
+</ol>
+
+<p>Total RNA was extracted with RNA STAT-60 (Tel-Test Inc.) according to the manufacturer's instructions. Briefly we:</p>
+
+<ol>
+ <li>homogenize tissue samples in the RNA STAT-60 (1 ml/50 to 100 mg tissue)</li>
+ <li>allowed the homogenate to stand for 5 min at room temperature</li>
+ <li>added 0.2 ml of chloroform per 1 ml RNA STAT-60</li>
+ <li>shook the sample vigorously for 15 sec and let the sample sit at room temperature for 3 min</li>
+ <li>centrifuged at 12,000 G for 15 min</li>
+ <li>transfered the aqueous phase to a fresh tube</li>
+ <li>added 0.5 ml of isopropanol per 1 ml RNA STAT-60</li>
+ <li>vortexed and allowed sample to stand at room temperature for 5-10 min</li>
+ <li>centrifuged at 12,000 G for 10-15 min</li>
+ <li>removed the supernatant and washed the RNA pellet with 75% ethanol</li>
+ <li>stored the pellet in 75% ethanol at -80 deg C until use</li>
+</ol>
+
+<p><strong>Sample Processing</strong>. Samples were processed in the VA Medical Center, Memphis, Rheumatology Disease Research Core Center lead by Drs. John Stuart and Weikuan Gu. All processing steps were performed by Dr. Yan Jiao. In brief, samples were purified using a standard sodium acetate in alcohol method (recommended by Affymetrix). The RNA quality was checked using a 1% agarose gel. The 18S and 28S bands had to be clear and the 28S band had to be more prominent. RNA concentation was measured using a spectrophotometer. The 260/280 ratios had to be greater than 1.7, and the majority were 1.8 or higher. We used a total of 8 micrograms of RNA as starting amount for cDNA synthesis using a standard Eberwine T7 polymerase method (Superscript II RT, Invitrogen Inc., Affy Part No 900431, GeneChip Expression 3' Amplification One-Cyle cDNA Synthesis Kit). The Affymetrix IVT labeling kit (Affy 900449) was used to generate labeled cRNA. At this point the cRNA was evaluated again using both the 260/280 ratio (values of 2.0 or above were acceptable) and 1% agarose gel inspection of the product (a size range from 200 to 7000 bp is considered suitable for use). We used 45 micrograms of labeled cRNA for fragmentation. Those samples that passed both QC steps (<10% usually fail) were then sheared using a fragmentation buffer included in the Affymetrix GeneChip Sample Cleanup Module (Part No.900371). After fragmentation, samples were either stored at -80 deg. centrigrade until use (one third roughly) or were immediately used for hybridization.</p>
+
+<p><strong>Dealing with Ocular Pigmentation</strong>: Variable ocular pigmentation is a potential confound in a study of the whole eye transcriptome. Even the most careful RNA preparations of brown and beige colored mice tend to have faint residual pigmentation that does affect hybridization signal. The key determinant of this interesting effect is the <em>Tyrp1</em> (brown) locus on Chr 5 at about 80 Mb. Loci on Chr 4 that map at this location should be considered with skepticism and reviewed carefully. To address this problem Yan Jiao purified total RNA a second time using the Qiagen RNeasy MinElute Cleanup Kit (Cat No. 74204). This was done for 8 colored samples (R2534E2, R2578E2, R1441E2, R2537E2, R2536E2, R2589E2, R2539E2), of which 5 were finally included in this data set (cases in which the strain ID is labeled with asterisks in the table).</p>
+
+<p><strong>Replication and Sample Balance:</strong> Our goal is to obtain data for independent biological sample pools from at least one of sample from each sex for all BXD strains. The current first batch of array data, represents a balanced sample of males and females, but without within-strain replication. We expect to add roughly 100 additional samples inthe next few months.</p>
+
+<p><strong>Batch Structure:</strong> This data set consists of a single batch. The great majority of arrays are from a single lot.</p>
+
+<p>The table below summarizes information on strain, age, sex, original CEL filename, several quality control values, and source of mice. Columns labeled "PDNN 2Z outlier" and "RMA 2Z outlier" list the fraction of probe sets with values that deviated more than 2 z units from the mean. Scale factor, background average, present, absent, marginal and 3'/5' ratios for actin and Gapdh were collated from the Affymetrix Report (RPT) files.</p>
+</blockquote>
+
+<blockquote>
+<table align="Center" border="1" cellpadding="0" cellspacing="0" style="width:75%">
+ <tbody>
+ <tr>
+ <td>
+ <table border="1" cellpadding="7" cellspacing="1" style="width:100%">
+ <tbody>
+ <tr>
+ <td>Id</td>
+ <td>tube ID</td>
+ <td>group type</td>
+ <td>Strain</td>
+ <td>age</td>
+ <td>sex</td>
+ <td>original CEL filename</td>
+ <td>PDNN 2Z outlier</td>
+ <td>RMA 2Z outlier</td>
+ <td>scale factor</td>
+ <td>background average</td>
+ <td>present</td>
+ <td>absent</td>
+ <td>marginal</td>
+ <td>AFFX-b-ActinMur(3'/5')</td>
+ <td>AFFX-GapdhMur(3'/5')</td>
+ <td>source</td>
+ </tr>
+ <tr>
+ <td>1</td>
+ <td>R2607E1</td>
+ <td>GDP BXD</td>
+ <td>C57BL/6J</td>
+ <td>67</td>
+ <td>F</td>
+ <td>R2605E.CEL</td>
+ <td>0.005</td>
+ <td>0.009</td>
+ <td>2.428</td>
+ <td>115.12</td>
+ <td>0.586</td>
+ <td>0.4</td>
+ <td>0.014</td>
+ <td>1.31</td>
+ <td>0.76</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>2</td>
+ <td>R0872E2</td>
+ <td>GDP BXD</td>
+ <td>C57BL/6J</td>
+ <td>66</td>
+ <td>M</td>
+ <td>R0872E.CEL</td>
+ <td>0.013</td>
+ <td>0.012</td>
+ <td>3.128</td>
+ <td>88.58</td>
+ <td>0.589</td>
+ <td>0.396</td>
+ <td>0.015</td>
+ <td>1.3</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>3</td>
+ <td>R2572E1</td>
+ <td>GDP BXD</td>
+ <td>DBA/2J</td>
+ <td>65</td>
+ <td>M</td>
+ <td>R2572E.CEL</td>
+ <td>0.041</td>
+ <td>0.051</td>
+ <td>2.406</td>
+ <td>79.07</td>
+ <td>0.555</td>
+ <td>0.429</td>
+ <td>0.016</td>
+ <td>1.37</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>4</td>
+ <td>R2601E1</td>
+ <td>GDP BXD</td>
+ <td>B6D2F1</td>
+ <td>73</td>
+ <td>F</td>
+ <td>R2601E.CEL</td>
+ <td>0.003</td>
+ <td>0.004</td>
+ <td>2.545</td>
+ <td>91.96</td>
+ <td>0.589</td>
+ <td>0.396</td>
+ <td>0.015</td>
+ <td>1.44</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>5</td>
+ <td>R2602E1</td>
+ <td>GDP BXD</td>
+ <td>B6D2F1</td>
+ <td>73</td>
+ <td>M</td>
+ <td>R2602E.CEL</td>
+ <td>0.001</td>
+ <td>0.004</td>
+ <td>2.599</td>
+ <td>84.44</td>
+ <td>0.597</td>
+ <td>0.388</td>
+ <td>0.015</td>
+ <td>1.37</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>6</td>
+ <td>R2600E1</td>
+ <td>GDP BXD</td>
+ <td>D2B6F1</td>
+ <td>72</td>
+ <td>F</td>
+ <td>R2600E.CEL</td>
+ <td>0.003</td>
+ <td>0.008</td>
+ <td>2.47</td>
+ <td>94.75</td>
+ <td>0.581</td>
+ <td>0.402</td>
+ <td>0.017</td>
+ <td>1.41</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>7</td>
+ <td>R2604E1</td>
+ <td>GDP BXD</td>
+ <td>D2B6F1</td>
+ <td>69</td>
+ <td>M</td>
+ <td>R2604E.CEL</td>
+ <td>0.003</td>
+ <td>0.007</td>
+ <td>2.657</td>
+ <td>89.63</td>
+ <td>0.594</td>
+ <td>0.392</td>
+ <td>0.015</td>
+ <td>1.28</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>8</td>
+ <td>R2597E1</td>
+ <td>BXD</td>
+ <td>BXD2</td>
+ <td>61</td>
+ <td>M</td>
+ <td>R2597E.CEL</td>
+ <td>0.003</td>
+ <td>0.007</td>
+ <td>2.374</td>
+ <td>93.56</td>
+ <td>0.603</td>
+ <td>0.383</td>
+ <td>0.015</td>
+ <td>1.34</td>
+ <td>0.77</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>9</td>
+ <td>R2591E1</td>
+ <td>BXD</td>
+ <td>BXD5</td>
+ <td>60</td>
+ <td>F</td>
+ <td>R2591E.CEL</td>
+ <td>0.051</td>
+ <td>0.009</td>
+ <td>1.7</td>
+ <td>136.48</td>
+ <td>0.585</td>
+ <td>0.4</td>
+ <td>0.015</td>
+ <td>1.33</td>
+ <td>0.78</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>10</td>
+ <td>R2570E1</td>
+ <td>BXD</td>
+ <td>BXD6</td>
+ <td>65</td>
+ <td>F</td>
+ <td>R2570E.CEL</td>
+ <td>0.002</td>
+ <td>0.006</td>
+ <td>1.987</td>
+ <td>86.73</td>
+ <td>0.585</td>
+ <td>0.4</td>
+ <td>0.015</td>
+ <td>1.46</td>
+ <td>0.76</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>11</td>
+ <td>R2538E1</td>
+ <td>BXD</td>
+ <td>BXD8</td>
+ <td>77</td>
+ <td>F</td>
+ <td>R2538E.CEL</td>
+ <td>0.037</td>
+ <td>0.028</td>
+ <td>1.905</td>
+ <td>101.98</td>
+ <td>0.612</td>
+ <td>0.373</td>
+ <td>0.015</td>
+ <td>1.52</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>12</td>
+ <td>R2569E1</td>
+ <td>BXD</td>
+ <td>BXD9</td>
+ <td>67</td>
+ <td>M</td>
+ <td>R2569E.CEL</td>
+ <td>0.014</td>
+ <td>0.027</td>
+ <td>1.753</td>
+ <td>87.36</td>
+ <td>0.551</td>
+ <td>0.434</td>
+ <td>0.015</td>
+ <td>2.82</td>
+ <td>3.14</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>13</td>
+ <td>R2581E1</td>
+ <td>BXD</td>
+ <td>BXD11</td>
+ <td>65</td>
+ <td>F</td>
+ <td>R2581E.CEL</td>
+ <td>0.006</td>
+ <td>0.012</td>
+ <td>1.941</td>
+ <td>88.55</td>
+ <td>0.621</td>
+ <td>0.364</td>
+ <td>0.016</td>
+ <td>1.55</td>
+ <td>0.81</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>14</td>
+ <td>R2543E1</td>
+ <td>BXD</td>
+ <td>BXD12</td>
+ <td>63</td>
+ <td>M</td>
+ <td>R2543E.CEL</td>
+ <td>0.036</td>
+ <td>0.007</td>
+ <td>1.605</td>
+ <td>117.69</td>
+ <td>0.586</td>
+ <td>0.399</td>
+ <td>0.016</td>
+ <td>1.43</td>
+ <td>0.77</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>15</td>
+ <td>R2586E1</td>
+ <td>BXD</td>
+ <td>BXD13</td>
+ <td>60</td>
+ <td>F</td>
+ <td>R2586E.CEL</td>
+ <td>0.020</td>
+ <td>0.035</td>
+ <td>2.006</td>
+ <td>73.61</td>
+ <td>0.564</td>
+ <td>0.42</td>
+ <td>0.016</td>
+ <td>2.85</td>
+ <td>3.81</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>16</td>
+ <td>R2557E1</td>
+ <td>BXD</td>
+ <td>BXD14</td>
+ <td>60</td>
+ <td>F</td>
+ <td>R2557E.CEL</td>
+ <td>0.014</td>
+ <td>0.017</td>
+ <td>1.83</td>
+ <td>98.76</td>
+ <td>0.625</td>
+ <td>0.361</td>
+ <td>0.014</td>
+ <td>1.31</td>
+ <td>0.78</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>17</td>
+ <td>R2567E1</td>
+ <td>BXD</td>
+ <td>BXD16</td>
+ <td>60</td>
+ <td>M</td>
+ <td>R2567E.CEL</td>
+ <td>0.016</td>
+ <td>0.025</td>
+ <td>2.239</td>
+ <td>82.35</td>
+ <td>0.567</td>
+ <td>0.416</td>
+ <td>0.017</td>
+ <td>1.37</td>
+ <td>0.75</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>18</td>
+ <td>R2559E1</td>
+ <td>BXD</td>
+ <td>BXD18</td>
+ <td>59</td>
+ <td>M</td>
+ <td>R2559E.CEL</td>
+ <td>0.035</td>
+ <td>0.006</td>
+ <td>1.654</td>
+ <td>103.68</td>
+ <td>0.608</td>
+ <td>0.377</td>
+ <td>0.015</td>
+ <td>1.27</td>
+ <td>0.78</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>19</td>
+ <td>R2560E1</td>
+ <td>BXD</td>
+ <td>BXD19</td>
+ <td>60</td>
+ <td>F</td>
+ <td>R2560E.CEL</td>
+ <td>0.026</td>
+ <td>0.007</td>
+ <td>1.792</td>
+ <td>98.33</td>
+ <td>0.609</td>
+ <td>0.375</td>
+ <td>0.016</td>
+ <td>1.35</td>
+ <td>0.8</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>20</td>
+ <td>R2584E1</td>
+ <td>BXD</td>
+ <td>BXD20</td>
+ <td>59</td>
+ <td>F</td>
+ <td>R2584E.CEL</td>
+ <td>0.003</td>
+ <td>0.007</td>
+ <td>2.07</td>
+ <td>83.82</td>
+ <td>0.593</td>
+ <td>0.391</td>
+ <td>0.016</td>
+ <td>1.4</td>
+ <td>0.76</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>21</td>
+ <td>R2541E2</td>
+ <td>BXD</td>
+ <td>BXD21</td>
+ <td>61</td>
+ <td>M</td>
+ <td>R2541E2.CEL</td>
+ <td>0.049</td>
+ <td>0.036</td>
+ <td>2.625</td>
+ <td>125.08</td>
+ <td>0.56</td>
+ <td>0.424</td>
+ <td>0.015</td>
+ <td>1.29</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>22</td>
+ <td>R2553E1</td>
+ <td>BXD</td>
+ <td>BXD22</td>
+ <td>58</td>
+ <td>F</td>
+ <td>R2553E.CEL</td>
+ <td>0.003</td>
+ <td>0.005</td>
+ <td>1.952</td>
+ <td>111.3</td>
+ <td>0.599</td>
+ <td>0.385</td>
+ <td>0.015</td>
+ <td>1.28</td>
+ <td>0.76</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>23</td>
+ <td>R2558E1</td>
+ <td>BXD</td>
+ <td>BXD23</td>
+ <td>60</td>
+ <td>F</td>
+ <td>R2558E2.CEL</td>
+ <td>0.013</td>
+ <td>0.015</td>
+ <td>1.908</td>
+ <td>114.53</td>
+ <td>0.599</td>
+ <td>0.388</td>
+ <td>0.014</td>
+ <td>1.2</td>
+ <td>0.82</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>24</td>
+ <td>R2589E2</td>
+ <td>BXD</td>
+ <td>BXD24-rd*</td>
+ <td>59</td>
+ <td>M</td>
+ <td>R2589E2.CEL</td>
+ <td>0.098</td>
+ <td>0.098</td>
+ <td>2.606</td>
+ <td>112.19</td>
+ <td>0.575</td>
+ <td>0.409</td>
+ <td>0.016</td>
+ <td>1.24</td>
+ <td>0.8</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>25</td>
+ <td>R2573E1</td>
+ <td>BXD</td>
+ <td>BXD25</td>
+ <td>67</td>
+ <td>F</td>
+ <td>R2573E2.CEL</td>
+ <td>0.009</td>
+ <td>0.018</td>
+ <td>3.153</td>
+ <td>71.88</td>
+ <td>0.579</td>
+ <td>0.407</td>
+ <td>0.014</td>
+ <td>1.77</td>
+ <td>0.97</td>
+ <td>UAB</td>
+ </tr>
+ <tr>
+ <td>26</td>
+ <td>R2562E1</td>
+ <td>BXD</td>
+ <td>BXD28</td>
+ <td>60</td>
+ <td>F</td>
+ <td>R2562E.CEL</td>
+ <td>0.003</td>
+ <td>0.005</td>
+ <td>1.649</td>
+ <td>116.35</td>
+ <td>0.599</td>
+ <td>0.384</td>
+ <td>0.017</td>
+ <td>1.37</td>
+ <td>0.79</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>27</td>
+ <td>R2561E1</td>
+ <td>BXD</td>
+ <td>BXD29</td>
+ <td>60</td>
+ <td>F</td>
+ <td>R2561E.CEL</td>
+ <td>0.019</td>
+ <td>0.029</td>
+ <td>1.952</td>
+ <td>93.32</td>
+ <td>0.583</td>
+ <td>0.402</td>
+ <td>0.015</td>
+ <td>2.19</td>
+ <td>1</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>28</td>
+ <td>R2598E1</td>
+ <td>BXD</td>
+ <td>BXD31</td>
+ <td>61</td>
+ <td>M</td>
+ <td>R2598E.CEL</td>
+ <td>0.003</td>
+ <td>0.006</td>
+ <td>1.989</td>
+ <td>106.48</td>
+ <td>0.609</td>
+ <td>0.376</td>
+ <td>0.015</td>
+ <td>1.27</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>29</td>
+ <td>R2563E1</td>
+ <td>BXD</td>
+ <td>BXD32</td>
+ <td>63</td>
+ <td>F</td>
+ <td>R2563E.CEL</td>
+ <td>0.008</td>
+ <td>0.011</td>
+ <td>1.547</td>
+ <td>101.52</td>
+ <td>0.619</td>
+ <td>0.367</td>
+ <td>0.014</td>
+ <td>1.5</td>
+ <td>0.8</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>30</td>
+ <td>R2542E1</td>
+ <td>BXD</td>
+ <td>BXD33</td>
+ <td>67</td>
+ <td>F</td>
+ <td>R2542E.CEL</td>
+ <td>0.010</td>
+ <td>0.016</td>
+ <td>2.128</td>
+ <td>97.08</td>
+ <td>0.565</td>
+ <td>0.418</td>
+ <td>0.016</td>
+ <td>1.91</td>
+ <td>0.93</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>31</td>
+ <td>R2585E1</td>
+ <td>BXD</td>
+ <td>BXD34</td>
+ <td>60</td>
+ <td>M</td>
+ <td>R2585E.CEL</td>
+ <td>0.007</td>
+ <td>0.014</td>
+ <td>2.64</td>
+ <td>75.13</td>
+ <td>0.583</td>
+ <td>0.4</td>
+ <td>0.017</td>
+ <td>1.25</td>
+ <td>0.77</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>32</td>
+ <td>R2532E1</td>
+ <td>BXD</td>
+ <td>BXD38</td>
+ <td>62</td>
+ <td>M</td>
+ <td>R2532E.CEL</td>
+ <td>0.002</td>
+ <td>0.003</td>
+ <td>2.038</td>
+ <td>93.65</td>
+ <td>0.598</td>
+ <td>0.387</td>
+ <td>0.015</td>
+ <td>1.37</td>
+ <td>0.8</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>33</td>
+ <td>R2574E1</td>
+ <td>BXD</td>
+ <td>BXD39</td>
+ <td>70</td>
+ <td>F</td>
+ <td>R2574E.CEL</td>
+ <td>0.001</td>
+ <td>0.004</td>
+ <td>1.981</td>
+ <td>90.64</td>
+ <td>0.612</td>
+ <td>0.373</td>
+ <td>0.015</td>
+ <td>1.39</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>34</td>
+ <td>R2590E1</td>
+ <td>BXD</td>
+ <td>BXD40</td>
+ <td>60</td>
+ <td>M</td>
+ <td>R2590E.CEL</td>
+ <td>0.004</td>
+ <td>0.007</td>
+ <td>2.708</td>
+ <td>77.3</td>
+ <td>0.591</td>
+ <td>0.393</td>
+ <td>0.015</td>
+ <td>1.4</td>
+ <td>0.77</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>35</td>
+ <td>R2596E1</td>
+ <td>BXD</td>
+ <td>BXD42</td>
+ <td>59</td>
+ <td>M</td>
+ <td>R2596E.CEL</td>
+ <td>0.013</td>
+ <td>0.017</td>
+ <td>2.632</td>
+ <td>108.46</td>
+ <td>0.59</td>
+ <td>0.396</td>
+ <td>0.015</td>
+ <td>1.24</td>
+ <td>0.8</td>
+ <td>Glenn</td>
+ </tr>
+ <tr>
+ <td>36</td>
+ <td>R2605E1</td>
+ <td>BXD</td>
+ <td>BXD43</td>
+ <td>79</td>
+ <td>M</td>
+ <td>R2607E.CEL</td>
+ <td>0.003</td>
+ <td>0.006</td>
+ <td>1.817</td>
+ <td>131.22</td>
+ <td>0.605</td>
+ <td>0.382</td>
+ <td>0.013</td>
+ <td>1.32</td>
+ <td>0.8</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>37</td>
+ <td>R2594E1</td>
+ <td>BXD</td>
+ <td>BXD44</td>
+ <td>63</td>
+ <td>F</td>
+ <td>R2594E.CEL</td>
+ <td>0.004</td>
+ <td>0.009</td>
+ <td>1.766</td>
+ <td>117.33</td>
+ <td>0.598</td>
+ <td>0.388</td>
+ <td>0.014</td>
+ <td>1.35</td>
+ <td>0.85</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>38</td>
+ <td>R2592E1</td>
+ <td>BXD</td>
+ <td>BXD45</td>
+ <td>62</td>
+ <td>M</td>
+ <td>R2592E.CEL</td>
+ <td>0.002</td>
+ <td>0.004</td>
+ <td>1.85</td>
+ <td>106.16</td>
+ <td>0.601</td>
+ <td>0.386</td>
+ <td>0.013</td>
+ <td>1.43</td>
+ <td>0.85</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>39</td>
+ <td>R2606E1</td>
+ <td>BXD</td>
+ <td>BXD48</td>
+ <td>78</td>
+ <td>M</td>
+ <td>R2606E.CEL</td>
+ <td>0.003</td>
+ <td>0.010</td>
+ <td>2.556</td>
+ <td>106.16</td>
+ <td>0.589</td>
+ <td>0.397</td>
+ <td>0.014</td>
+ <td>1.35</td>
+ <td>0.83</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>40</td>
+ <td>R2603E1</td>
+ <td>BXD</td>
+ <td>BXD51</td>
+ <td>66</td>
+ <td>F</td>
+ <td>R2603E.CEL</td>
+ <td>0.003</td>
+ <td>0.009</td>
+ <td>2.488</td>
+ <td>115.16</td>
+ <td>0.577</td>
+ <td>0.408</td>
+ <td>0.015</td>
+ <td>1.24</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>41</td>
+ <td>R2534E2</td>
+ <td>BXD</td>
+ <td>BXD61*</td>
+ <td>70</td>
+ <td>F</td>
+ <td>R2534E2.CEL</td>
+ <td>0.030</td>
+ <td>0.028</td>
+ <td>2.473</td>
+ <td>117.76</td>
+ <td>0.579</td>
+ <td>0.406</td>
+ <td>0.015</td>
+ <td>1.42</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>42</td>
+ <td>R2611E1</td>
+ <td>BXD</td>
+ <td>BXD64</td>
+ <td>68</td>
+ <td>M</td>
+ <td>R2611E.CEL</td>
+ <td>0.013</td>
+ <td>0.022</td>
+ <td>2.292</td>
+ <td>91.99</td>
+ <td>0.58</td>
+ <td>0.405</td>
+ <td>0.015</td>
+ <td>1.57</td>
+ <td>1.06</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>43</td>
+ <td>R2583E1</td>
+ <td>BXD</td>
+ <td>BXD65</td>
+ <td>60</td>
+ <td>M</td>
+ <td>R2583E.CEL</td>
+ <td>0.005</td>
+ <td>0.010</td>
+ <td>2.492</td>
+ <td>70.43</td>
+ <td>0.569</td>
+ <td>0.415</td>
+ <td>0.016</td>
+ <td>1.67</td>
+ <td>1.01</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>44</td>
+ <td>R2536E2</td>
+ <td>BXD</td>
+ <td>BXD66*</td>
+ <td>64</td>
+ <td>F</td>
+ <td>R2536E2.CEL</td>
+ <td>0.039</td>
+ <td>0.065</td>
+ <td>2.74</td>
+ <td>108.62</td>
+ <td>0.561</td>
+ <td>0.423</td>
+ <td>0.017</td>
+ <td>1.28</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>45</td>
+ <td>R2551E1</td>
+ <td>BXD</td>
+ <td>BXD68</td>
+ <td>67</td>
+ <td>F</td>
+ <td>R2551E.CEL</td>
+ <td>0.037</td>
+ <td>0.039</td>
+ <td>2.493</td>
+ <td>92.38</td>
+ <td>0.543</td>
+ <td>0.441</td>
+ <td>0.016</td>
+ <td>2.91</td>
+ <td>1.55</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>46</td>
+ <td>R2593E1</td>
+ <td>BXD</td>
+ <td>BXD69</td>
+ <td>59</td>
+ <td>F</td>
+ <td>R2593E.CEL</td>
+ <td>0.008</td>
+ <td>0.013</td>
+ <td>1.672</td>
+ <td>127.6</td>
+ <td>0.592</td>
+ <td>0.395</td>
+ <td>0.013</td>
+ <td>1.47</td>
+ <td>0.92</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>47</td>
+ <td>R2537E2</td>
+ <td>BXD</td>
+ <td>BXD70*</td>
+ <td>59</td>
+ <td>M</td>
+ <td>R2537E2.CEL</td>
+ <td>0.046</td>
+ <td>0.044</td>
+ <td>2.93</td>
+ <td>98.66</td>
+ <td>0.58</td>
+ <td>0.405</td>
+ <td>0.016</td>
+ <td>1.29</td>
+ <td>0.75</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>48</td>
+ <td>R2565E1</td>
+ <td>BXD</td>
+ <td>BXD75</td>
+ <td>61</td>
+ <td>F</td>
+ <td>R2565E.CEL</td>
+ <td>0.009</td>
+ <td>0.017</td>
+ <td>1.79</td>
+ <td>101.68</td>
+ <td>0.58</td>
+ <td>0.405</td>
+ <td>0.015</td>
+ <td>2.31</td>
+ <td>3.47</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>49</td>
+ <td>R2579E1</td>
+ <td>BXD</td>
+ <td>BXD80</td>
+ <td>65</td>
+ <td>F</td>
+ <td>R2579E.CEL</td>
+ <td>0.005</td>
+ <td>0.010</td>
+ <td>2.419</td>
+ <td>72.13</td>
+ <td>0.592</td>
+ <td>0.394</td>
+ <td>0.015</td>
+ <td>1.73</td>
+ <td>0.82</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>50</td>
+ <td>R2540E1</td>
+ <td>BXD</td>
+ <td>BXD87</td>
+ <td>63</td>
+ <td>M</td>
+ <td>R2540E.CEL</td>
+ <td>0.013</td>
+ <td>0.016</td>
+ <td>2.333</td>
+ <td>93.15</td>
+ <td>0.611</td>
+ <td>0.374</td>
+ <td>0.014</td>
+ <td>1.22</td>
+ <td>0.81</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>51</td>
+ <td>R2545E1</td>
+ <td>BXD</td>
+ <td>BXD89</td>
+ <td>67</td>
+ <td>M</td>
+ <td>R2546E.CEL</td>
+ <td>0.046</td>
+ <td>0.046</td>
+ <td>1.667</td>
+ <td>104.76</td>
+ <td>0.562</td>
+ <td>0.423</td>
+ <td>0.015</td>
+ <td>3.6</td>
+ <td>9.84</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>52</td>
+ <td>R2578E2</td>
+ <td>BXD</td>
+ <td>BXD90*</td>
+ <td>61</td>
+ <td>F</td>
+ <td>R2578E2.CEL</td>
+ <td>0.033</td>
+ <td>0.034</td>
+ <td>2.785</td>
+ <td>92.27</td>
+ <td>0.586</td>
+ <td>0.398</td>
+ <td>0.016</td>
+ <td>1.52</td>
+ <td>0.77</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>53</td>
+ <td>R2554E1</td>
+ <td>BXD</td>
+ <td>BXD96</td>
+ <td>67</td>
+ <td>M</td>
+ <td>R2554E.CEL</td>
+ <td>0.004</td>
+ <td>0.004</td>
+ <td>2.177</td>
+ <td>93.02</td>
+ <td>0.602</td>
+ <td>0.383</td>
+ <td>0.015</td>
+ <td>1.46</td>
+ <td>0.77</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>54</td>
+ <td>R2577E1</td>
+ <td>BXD</td>
+ <td>BXD97</td>
+ <td>55</td>
+ <td>M</td>
+ <td>R2577E.CEL</td>
+ <td>0.019</td>
+ <td>0.016</td>
+ <td>2.07</td>
+ <td>76.58</td>
+ <td>0.595</td>
+ <td>0.391</td>
+ <td>0.014</td>
+ <td>1.87</td>
+ <td>1.29</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>55</td>
+ <td>R2595E1</td>
+ <td>GDP</td>
+ <td>129S1/SvImJ</td>
+ <td>59</td>
+ <td>F</td>
+ <td>R2595E.CEL</td>
+ <td>0.017</td>
+ <td>0.021</td>
+ <td>1.792</td>
+ <td>115.39</td>
+ <td>0.61</td>
+ <td>0.375</td>
+ <td>0.015</td>
+ <td>1.46</td>
+ <td>0.77</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>56</td>
+ <td>R2533E1</td>
+ <td>GDP</td>
+ <td>129S1/SvImJ</td>
+ <td>60</td>
+ <td>M</td>
+ <td>R2533E.CEL</td>
+ <td>0.021</td>
+ <td>0.013</td>
+ <td>2.107</td>
+ <td>93.55</td>
+ <td>0.579</td>
+ <td>0.405</td>
+ <td>0.016</td>
+ <td>1.37</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>57</td>
+ <td>R2546E1</td>
+ <td>GDP</td>
+ <td>A/J</td>
+ <td>66</td>
+ <td>F</td>
+ <td>R2545E.CEL</td>
+ <td>0.018</td>
+ <td>0.014</td>
+ <td>1.989</td>
+ <td>95.59</td>
+ <td>0.586</td>
+ <td>0.397</td>
+ <td>0.017</td>
+ <td>1.47</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>58</td>
+ <td>R0754E2</td>
+ <td>GDP</td>
+ <td>A/J</td>
+ <td>60</td>
+ <td>M</td>
+ <td>R0754E.CEL</td>
+ <td>0.014</td>
+ <td>0.016</td>
+ <td>2.718</td>
+ <td>85.63</td>
+ <td>0.598</td>
+ <td>0.387</td>
+ <td>0.015</td>
+ <td>1.36</td>
+ <td>0.76</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>59</td>
+ <td>R1676E1</td>
+ <td>GDP</td>
+ <td>BALB/cByJ</td>
+ <td>83</td>
+ <td>F</td>
+ <td>R1676E.CEL</td>
+ <td>0.042</td>
+ <td>0.041</td>
+ <td>2.685</td>
+ <td>98.37</td>
+ <td>0.589</td>
+ <td>0.396</td>
+ <td>0.015</td>
+ <td>1.46</td>
+ <td>0.74</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>60</td>
+ <td>R1672E1</td>
+ <td>GDP</td>
+ <td>BALB/cByJ</td>
+ <td>83</td>
+ <td>M</td>
+ <td>R1672E.CEL</td>
+ <td>0.022</td>
+ <td>0.022</td>
+ <td>2.216</td>
+ <td>110.52</td>
+ <td>0.599</td>
+ <td>0.386</td>
+ <td>0.015</td>
+ <td>1.26</td>
+ <td>0.8</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>61</td>
+ <td>R1700E1</td>
+ <td>GDP</td>
+ <td>C3H/HeJ</td>
+ <td>83</td>
+ <td>F</td>
+ <td>R1700E.CEL</td>
+ <td>0.090</td>
+ <td>0.092</td>
+ <td>2.978</td>
+ <td>68.77</td>
+ <td>0.608</td>
+ <td>0.379</td>
+ <td>0.014</td>
+ <td>1.48</td>
+ <td>0.78</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>62</td>
+ <td>R1704E1</td>
+ <td>GDP</td>
+ <td>C3H/HeJ</td>
+ <td>83</td>
+ <td>M</td>
+ <td>R1704E.CEL</td>
+ <td>0.086</td>
+ <td>0.089</td>
+ <td>2.581</td>
+ <td>88.29</td>
+ <td>0.601</td>
+ <td>0.386</td>
+ <td>0.013</td>
+ <td>1.38</td>
+ <td>0.84</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>63</td>
+ <td>R2564E1</td>
+ <td>GDP</td>
+ <td>CAST/Ei</td>
+ <td>64</td>
+ <td>F</td>
+ <td>R2564E.CEL</td>
+ <td>0.078</td>
+ <td>0.064</td>
+ <td>1.937</td>
+ <td>88.89</td>
+ <td>0.585</td>
+ <td>0.399</td>
+ <td>0.016</td>
+ <td>1.6</td>
+ <td>0.77</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>64</td>
+ <td>R2580E1</td>
+ <td>GDP</td>
+ <td>CAST/Ei</td>
+ <td>64</td>
+ <td>M</td>
+ <td>R2580E.CEL</td>
+ <td>0.076</td>
+ <td>0.067</td>
+ <td>2.089</td>
+ <td>94.64</td>
+ <td>0.582</td>
+ <td>0.401</td>
+ <td>0.017</td>
+ <td>1.4</td>
+ <td>0.76</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>65</td>
+ <td>R2636E1</td>
+ <td>GDP</td>
+ <td>KK/HIJ</td>
+ <td>64</td>
+ <td>F</td>
+ <td>R2636E.CEL</td>
+ <td>0.023</td>
+ <td>0.026</td>
+ <td>2.61</td>
+ <td>93.1</td>
+ <td>0.589</td>
+ <td>0.395</td>
+ <td>0.015</td>
+ <td>1.39</td>
+ <td>0.76</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>66</td>
+ <td>R2637E1</td>
+ <td>GDP</td>
+ <td>KK/HIJ</td>
+ <td>64</td>
+ <td>M</td>
+ <td>R2637E.CEL</td>
+ <td>0.039</td>
+ <td>0.020</td>
+ <td>2.189</td>
+ <td>102.78</td>
+ <td>0.594</td>
+ <td>0.39</td>
+ <td>0.015</td>
+ <td>1.3</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>67</td>
+ <td>R0999E1</td>
+ <td>GDP</td>
+ <td>LG/J</td>
+ <td>57</td>
+ <td>F</td>
+ <td>R0999E.CEL</td>
+ <td>0.012</td>
+ <td>0.012</td>
+ <td>2.448</td>
+ <td>82.09</td>
+ <td>0.594</td>
+ <td>0.391</td>
+ <td>0.015</td>
+ <td>1.38</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>68</td>
+ <td>R1004E1</td>
+ <td>GDP</td>
+ <td>LG/J</td>
+ <td>65</td>
+ <td>M</td>
+ <td>R1004E.CEL</td>
+ <td>0.013</td>
+ <td>0.015</td>
+ <td>2.438</td>
+ <td>91.71</td>
+ <td>0.587</td>
+ <td>0.398</td>
+ <td>0.015</td>
+ <td>1.38</td>
+ <td>0.79</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>69</td>
+ <td>R1688E1</td>
+ <td>GDP</td>
+ <td>NOD/LtJ</td>
+ <td>66</td>
+ <td>F</td>
+ <td>R1688E.CEL</td>
+ <td>0.017</td>
+ <td>0.019</td>
+ <td>2.664</td>
+ <td>97.65</td>
+ <td>0.586</td>
+ <td>0.399</td>
+ <td>0.015</td>
+ <td>1.26</td>
+ <td>0.8</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>70</td>
+ <td>R2566E1</td>
+ <td>GDP</td>
+ <td>NOD/LtJ</td>
+ <td>76</td>
+ <td>M</td>
+ <td>R2566E2.CEL</td>
+ <td>0.019</td>
+ <td>0.025</td>
+ <td>3.031</td>
+ <td>69.44</td>
+ <td>0.598</td>
+ <td>0.388</td>
+ <td>0.015</td>
+ <td>1.38</td>
+ <td>0.75</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>71</td>
+ <td>R2550E1</td>
+ <td>GDP</td>
+ <td>NZO/HlLtJ</td>
+ <td>96</td>
+ <td>M</td>
+ <td>R2550E.CEL</td>
+ <td>0.023</td>
+ <td>0.015</td>
+ <td>1.794</td>
+ <td>87.16</td>
+ <td>0.607</td>
+ <td>0.378</td>
+ <td>0.015</td>
+ <td>1.52</td>
+ <td>0.82</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>72</td>
+ <td>R2535E1</td>
+ <td>GDP</td>
+ <td>NZO/HlLtJ</td>
+ <td>62</td>
+ <td>F</td>
+ <td>R2535E.CEL</td>
+ <td>0.046</td>
+ <td>0.025</td>
+ <td>1.893</td>
+ <td>85.67</td>
+ <td>0.604</td>
+ <td>0.382</td>
+ <td>0.014</td>
+ <td>1.41</td>
+ <td>0.85</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>73</td>
+ <td>R2634E1</td>
+ <td>GDP</td>
+ <td>PWD/PhJ</td>
+ <td>62</td>
+ <td>F</td>
+ <td>R2635E.CEL</td>
+ <td>0.077</td>
+ <td>0.069</td>
+ <td>3.292</td>
+ <td>89.8</td>
+ <td>0.559</td>
+ <td>0.425</td>
+ <td>0.016</td>
+ <td>1.57</td>
+ <td>0.81</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>74</td>
+ <td>R2635E1</td>
+ <td>GDP</td>
+ <td>PWD/PhJ</td>
+ <td>62</td>
+ <td>M</td>
+ <td>R2634E.CEL</td>
+ <td>0.088</td>
+ <td>0.081</td>
+ <td>3.722</td>
+ <td>80.05</td>
+ <td>0.542</td>
+ <td>0.441</td>
+ <td>0.017</td>
+ <td>1.53</td>
+ <td>0.85</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>75</td>
+ <td>R2544E1</td>
+ <td>GDP</td>
+ <td>PWK/PhJ</td>
+ <td>63</td>
+ <td>F</td>
+ <td>R2544E.CEL</td>
+ <td>0.106</td>
+ <td>0.100</td>
+ <td>2.196</td>
+ <td>107.51</td>
+ <td>0.549</td>
+ <td>0.435</td>
+ <td>0.017</td>
+ <td>1.36</td>
+ <td>0.82</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>76</td>
+ <td>R2549E1</td>
+ <td>GDP</td>
+ <td>PWK/PhJ</td>
+ <td>83</td>
+ <td>M</td>
+ <td>R2549E.CEL</td>
+ <td>0.065</td>
+ <td>0.048</td>
+ <td>2.275</td>
+ <td>83.8</td>
+ <td>0.573</td>
+ <td>0.412</td>
+ <td>0.015</td>
+ <td>1.57</td>
+ <td>0.83</td>
+ <td>JAX</td>
+ </tr>
+ <tr>
+ <td>77</td>
+ <td>R2368E1</td>
+ <td>GDP</td>
+ <td>WSB/EiJ</td>
+ <td>67</td>
+ <td>F</td>
+ <td>R2368E.CEL</td>
+ <td>0.025</td>
+ <td>0.028</td>
+ <td>2.567</td>
+ <td>85.7</td>
+ <td>0.595</td>
+ <td>0.391</td>
+ <td>0.014</td>
+ <td>1.29</td>
+ <td>0.74</td>
+ <td>UTM RW</td>
+ </tr>
+ <tr>
+ <td>78</td>
+ <td>R2547E1</td>
+ <td>GDP</td>
+ <td>WSB/EiJ</td>
+ <td>67</td>
+ <td>M</td>
+ <td>R2547E.CEL</td>
+ <td>0.032</td>
+ <td>0.021</td>
+ <td>2.135</td>
+ <td>90.04</td>
+ <td>0.582</td>
+ <td>0.401</td>
+ <td>0.016</td>
+ <td>1.32</td>
+ <td>0.77</td>
+ <td>UTM RW</td>
+ </tr>
+ </tbody>
+ </table>
+ </td>
+ </tr>
+ </tbody>
+</table>
+</blockquote>
|