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authorBonface2024-02-13 23:52:26 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commitb2feda451ccfbeaed02dce9088d6dd228cf15861 (patch)
tree3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/El_bxdcdhfdscwat_0216/tissue.rtf
parentd029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff)
downloadgn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz
Update dataset RTF Files.
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+<blockquote>
+<table border="0" cellpadding="5" cellspacing="0" style="width:100%">
+ <tbody>
+ <tr>
+ <td>
+ <p>Subcuteaneous WAT was later shattered in liquid nitrogen, and around 100 mg was taken for preparation. All ~5 animals per cohort had their RNA prepared, and then were pooled evenly (by &micro;g of RNA) into a single RNA sample for each cohort. The pooled RNA samples were then purified using RNEasy, then sent out for array analysis. All RIN values were &gt; 8.0. RNA was prepared in the summer of 2013, while the RNEasy cleanup occurred in the winter of 2015; unlike the other tissues run as a part of this study, these RNA samples spent a significant time in the -80&deg; freezers. However, the samples only underwent one freeze&mdash;thaw cycle.</p>
+ </td>
+ </tr>
+ </tbody>
+</table>
+</blockquote>