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authorBonface2024-02-09 09:41:28 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commitd029d5d7f8ead1f1de8d318045004a4a6f68f5fb (patch)
tree33c7ff40e3f953d030ed08f468f7afb1dfcba9e6 /general/datasets/EPFL_LISPBXDHeCD0114/tissue.rtf
parent769ff7825f5d8d36d541e90534c07f1985899973 (diff)
downloadgn-docs-d029d5d7f8ead1f1de8d318045004a4a6f68f5fb.tar.gz
Update dataset RTF Files.
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+<p>Hearts were later shattered in liquid nitrogen, usually broken into 2-4 pieces, and around half of the sample (at random) was taken for preparation. All ~5 animals per cohort had their RNA prepared, and then were pooled evenly (by &micro;g of RNA) into a single RNA sample for each cohort, thus differences due to different segments of the heart being taken should be mitigated, although not eliminated, and researchers examining extremely specific transcriptional regulation in the heart should keep this in mind. The pooled RNA samples were then purified using RNEasy, then sent out for array analysis. All RIN values were &gt; 8.0.</p>