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authorBonface2024-02-13 23:52:26 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commitb2feda451ccfbeaed02dce9088d6dd228cf15861 (patch)
tree3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/Br_u_1203_m/summary.rtf
parentd029d5d7f8ead1f1de8d318045004a4a6f68f5fb (diff)
downloadgn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz
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+<p>This August 2005 data freeze provides estimates of mRNA expression in brains of BXD recombinant inbred mice measured using Affymetrix U74Av2 microarrays. Data were generated at the University of Tennessee Health Science Center (UTHSC). Over 300 brain samples from 35 strains were hybridized in small pools (n=3) to 97 arrays. Data were processed using the Microarray Suite 5 (<a class="fs14" href="www.affymetrix.com/support/technical/whitepapers/sadd_whitepaper.pdf">MAS 5</a>) protocol of Affymetrix. To simplify comparison between transforms, MAS 5 values of each array were log2 transformed and adjusted to an average of 8 units. In general, MAS 5 data do not perform as well as RMA, PDNN, or the new heritability weighted transforms (HW1PM).</p>