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authorBonface2024-02-09 09:41:28 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
commitd029d5d7f8ead1f1de8d318045004a4a6f68f5fb (patch)
tree33c7ff40e3f953d030ed08f468f7afb1dfcba9e6 /general/datasets/Br_U_0503_M/summary.rtf
parent769ff7825f5d8d36d541e90534c07f1985899973 (diff)
downloadgn-docs-d029d5d7f8ead1f1de8d318045004a4a6f68f5fb.tar.gz
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+<p>This May 2003 freeze provides estimates of mRNA expression in brains of BXD recombinant inbred mice measured using Affymetrix U74Av2 microarrays. New users are encouraged to use one of the more recent data sets (December 2003 or March 2004). Data were generated at the University of Tennessee Health Science Center (UTHSC). Over 300 brain samples from 33 strains were hybridized in small pools (n=3) to 97 arrays. Data were processed using the Microarray Suite 5 (<a class="fs14" href="www.affymetrix.com/support/technical/whitepapers/sadd_whitepaper.pdf">MAS 5</a>) protocol of Affymetrix. To simplify comparison between transforms, MAS 5 values of each array were adjusted to an average of 8 units and a variance of 2 units. In general, these MAS 5 transforms do not perform as well as RMA, PDNN, or the new heritability weighted transforms (HW1PM).</p>