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authorAlexander_Kabui2024-11-11 14:58:40 +0300
committerAlexander_Kabui2024-11-11 14:58:40 +0300
commit8dc26d3907374198bdea28cd7b0a0499cfb96557 (patch)
tree18f5daefee9e987a453b6e8afca778a9344d9747 /scripts
parent172f8c30dadc5cb8517112939202c6ced94361c2 (diff)
downloadgenenetwork3-8dc26d3907374198bdea28cd7b0a0499cfb96557.tar.gz
refactor: rename dataset to cross.
Diffstat (limited to 'scripts')
-rw-r--r--scripts/rqtl2_wrapper.R82
1 files changed, 41 insertions, 41 deletions
diff --git a/scripts/rqtl2_wrapper.R b/scripts/rqtl2_wrapper.R
index 753a88d..5908934 100644
--- a/scripts/rqtl2_wrapper.R
+++ b/scripts/rqtl2_wrapper.R
@@ -126,32 +126,32 @@ cat("Generating the cross object at ", control_file_path, "\n")
generate_cross_object(control_file_path, json_data)
cat("reading the cross object from", control_file_path, "\n")
-dataset <- read_cross2(control_file_path, quiet = FALSE) # replace this with a dynamic path
+cross <- read_cross2(control_file_path, quiet = FALSE) # replace this with a dynamic path
# check integrity of the cross
cat("Check the integrity of the cross object")
-check_cross2(dataset)
-if (check_cross2(dataset)) {
- print("Dataset meets required specifications for a cross")
+check_cross2(cross)
+if (check_cross2(cross)) {
+ print("Cross meets required specifications for a cross")
} else {
- print("Dataset does not meet required specifications")
+ print("Cross does not meet required specifications")
}
-# Dataset Summarys
-cat("A Summary about the Dataset You Provided\n")
-summary(dataset)
-n_ind(dataset)
-n_chr(dataset)
+# Cross Summarys
+cat("A Summary about the Cross You Provided\n")
+summary(cross)
+n_ind(cross)
+n_chr(cross)
cat("names of markers in the object\n")
-marker_names(dataset)
+marker_names(cross)
cat("names of phenotypes in a the object")
-pheno_names(dataset)
-cat("IDs for all individuals in the dataset cross object that have genotype data\n")
-ind_ids_geno(dataset)
-cat(" IDs for all individuals in the dataset object that have phenotype data")
-ind_ids_pheno(dataset)
+pheno_names(cross)
+cat("IDs for all individuals in the cross cross object that have genotype data\n")
+ind_ids_geno(cross)
+cat(" IDs for all individuals in the cross object that have phenotype data")
+ind_ids_pheno(cross)
cat("Name of the founder Strains/n")
-founders(dataset)
+founders(cross)
# Function for computing the genetic probabilities
@@ -185,11 +185,11 @@ perform_genetic_pr <- function(cross,
# Step: calculate the genetic probabilities
cat("Calculating the genetic probabilities\n")
-Pr = perform_genetic_pr(dataset)
+Pr = perform_genetic_pr(cross)
# Step: perform allele probabilites if cross ways
-if (dataset$crosstype == "4way"){
+if (cross$crosstype == "4way"){
cat("Calculating Allele Genetic probability for 4way cross\n")
aPr <- genoprob_to_alleleprob(pr)
}
@@ -198,7 +198,7 @@ if (dataset$crosstype == "4way"){
#Function to Calculate genotyping error LOD scores
cat("Calculating the genotype error LOD scores\n")
-error_lod <- calc_errorlod(dataset, Pr, quiet = FALSE, cores = NO_OF_CORES)
+error_lod <- calc_errorlod(cross, Pr, quiet = FALSE, cores = NO_OF_CORES)
# combine into one matrix
error_lod <- do.call("cbind", error_lod)
@@ -208,16 +208,16 @@ error_lod <- do.call("cbind", error_lod)
## grab phenotypes and covariates; ensure that covariates have names attribute
# TODO rework on this
cat("Getting the phenotypes and covariates\n")
-pheno <- dataset$pheno
+pheno <- cross$pheno
-covar <- match(dataset$covar$sex, c("f", "m")) # make numeric
+covar <- match(cross$covar$sex, c("f", "m")) # make numeric
if (!is.null(covar)){
- names(covar) <- rownames(dataset$covar)
+ names(covar) <- rownames(cross$covar)
}
print("The covariates are")
print(covar)
-Xcovar <- get_x_covar(dataset)
+Xcovar <- get_x_covar(cross)
print("The Xcovar are ")
print(Xcovar)
@@ -234,7 +234,7 @@ if (opt$method == "LMM"){
cat("Calculating the kinship for the genetic probability\n")
-if (dataset$crosstype == "4way"){
+if (cross$crosstype == "4way"){
kinship <- get_kinship(aPr, opt$method)
} else {
kinship <- get_kinship(Pr, "loco")
@@ -307,13 +307,13 @@ perform_genome_scan <- function(cross,
}
# Perform the genome scan for the cross object
-if (dataset$crosstype == "4way"){
+if (cross$crosstype == "4way"){
sex <- (DOex$covar$Sex == "male")*1
- names(sex) <- rownames(dataset$covar)
- sex <- setNames( (dataset$covar$Sex == "male")*1, rownames(DOex$covar))
- scan_results <- perform_genome_scan(aPr, dataset, kinship=kinship, method = "LOCO", addcovar = sex)
+ names(sex) <- rownames(cross$covar)
+ sex <- setNames( (cross$covar$Sex == "male")*1, rownames(DOex$covar))
+ scan_results <- perform_genome_scan(aPr, cross, kinship=kinship, method = "LOCO", addcovar = sex)
} else {
- scan_results <- perform_genome_scan(cross = dataset,
+ scan_results <- perform_genome_scan(cross = cross,
genome_prob = Pr,
kinship = kinship,
method = SCAN_METHOD)
@@ -357,7 +357,7 @@ generate_lod_plot <- function(cross, scan_result, method, base_dir = ".") {
}
-lod_plot_path <- generate_lod_plot(dataset, scan_results, "HK", base_dir=opt$directory)
+lod_plot_path <- generate_lod_plot(cross, scan_results, "HK", base_dir=opt$directory)
cat("Generated the lod plot at ", lod_plot_path, "\n")
@@ -417,7 +417,7 @@ perm_strata <- NULL
}
# Step: Performing the permutation test
-perm <- perform_permutation_test(dataset, Pr, n_perm = NO_OF_PERMUTATION,perm_strata = perm_strata, method = opt$method)
+perm <- perform_permutation_test(cross, Pr, n_perm = NO_OF_PERMUTATION,perm_strata = perm_strata, method = opt$method)
# get the permutation summary with a significance threshold
@@ -436,7 +436,7 @@ cat("Fetching the lod peaks with threshold", opt$threshold, "\n")
lod_peaks = find_peaks(
scan_results,
threshold =opt$threshold,
- map = dataset$gmap,
+ map = cross$gmap,
cores = NO_OF_CORES
)
@@ -461,12 +461,12 @@ get_qtl_effect <- function(chromosome,geno_prob,pheno,covar=NULL,LOCO= NULL){
-# take the first phenotype in the dataset
+# take the first phenotype in the cross
# grab phenotypes and covariates; ensure that covariates have names attribute
-pheno <- dataset$pheno[,1]
-if (!is.null(dataset$covar) && !is.null(dataset$covar$sex)){
- covar <- match(dataset$covar$sex, c("f", "m")) # make numeric
- names(covar) <- rownames(dataset$covar)
+pheno <- cross$pheno[,1]
+if (!is.null(cross$covar) && !is.null(cross$covar$sex)){
+ covar <- match(cross$covar$sex, c("f", "m")) # make numeric
+ names(covar) <- rownames(cross$covar)
} else {
covar <- NULL
}
@@ -474,9 +474,9 @@ covar <- NULL
meffects <- c()
meffects_plots <- c()
# TODO add plots for meffects
-for (chr in chr_names(dataset)){
+for (chr in chr_names(cross)){
cat("Getting the qtl effect for chromosome", chr, "\n")
- if (dataset$crosstype == "4way"){
+ if (cross$crosstype == "4way"){
coeff_results <- get_qtl_effect(chr, aPr, pheno, LOCO="LOCO", covar = sex)
cat("Generating the qtl effects plots\n")
file_name = genRandomFileName(prefix = "RQTL_EFFECT_", file_ext = ".png")
@@ -504,7 +504,7 @@ output = list(lod_peaks = lod_peaks,
lod_significance = lod_significance,
permutation_results = perm,
lod_peaks = lod_peaks,
- chromosomes = chr_names(dataset),
+ chromosomes = chr_names(cross),
meffects = meffects,
error_lod = error_lod,
meffects_plots = meffects_plots,