1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
|
<html>
<head>
<meta http-equiv=Content-Type content="text/html; charset=macintosh">
<meta name=ProgId content=PowerPoint.Slide>
<meta name=Generator content="Microsoft Macintosh PowerPoint 11">
<link id=Main-File rel=Main-File href="webqtl_demo2.ppt.htm">
<title>PowerPoint Presentation - Complex trait analysis, develop-ment, and
genomics</title>
<link title="Presentation File" type="application/powerpoint" rel=alternate
href="webqtl_demo2.ppt.ppt">
<script>
if ( ! top.PPTPRESENTATION ) {
window.location.replace( "endshow.htm" );
}
</script>
<meta name=Description
content="Aug-15-05: Evaluating Ctbp2 as a candidate QTL for App">
<link rel=Stylesheet href="master03_stylesheet.css">
<style media=print>
<!--.sld
{left:0px !important;
width:6.0in !important;
height:4.5in !important;
font-size:76% !important;}
-->
</style>
<script language=JavaScript src=script.js></script><script language=JavaScript><!--
gId="slide0010.htm"
if ( !parent.base.g_done && (parent.base.msie < 0 ) ) {
parent.base.g_done = 1;
document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref);
}
if( !IsNts() ) Redirect( "PPTSld", gId );
var g_hasTrans = true, g_autoTrans = false, g_transSecs = 1;
//-->
</script><script for=window event=onload language=JavaScript><!--
if( !IsSldOrNts() ) return;
if( MakeNotesVis() ) return;
LoadSld( gId );
playList();MakeSldVis(1);
//-->
</script>
</head>
<body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)"
onresize="_RSW()" onkeypress="_KPH(event)">
<div id=SlideObj class=sld style='position:absolute;top:0px;left:0px;
width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%);
visibility:hidden'><img src="master03_background.png" v:shapes="_x0000_s1026"
style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'>
<div style='position:absolute;top:0%;left:1.72%;width:106.35%;height:7.24%;
filter:DropShadow(Color=#000000, OffX=2, OffY=2)'>
<div class=T style='mso-line-spacing:"-358 0 -1";mso-margin-left-alt:233;
mso-text-indent-alt:0;position:absolute;top:12.19%;left:.87%;width:99.12%;
height:90.24%'><span style='font-family:Verdana;font-size:64%'>Evaluating </span><span
style='font-family:Verdana;font-size:64%'><i>Ctbp2</i></span><span
style='font-family:Verdana;font-size:64%'> as a candidate QTL for </span><span
style='font-family:Verdana;font-size:64%'><i>App</i></span><span
style='font-family:Verdana;font-size:73%;mso-special-format:lastCR;display:
none'><i><br>
</i></span></div>
</div>
<img border=0 src="slide0010_image082.png" style='position:absolute;top:94.52%;
left:3.31%;width:96.68%;height:4.24%'><img border=0 src="slide0010_image083.png"
style='position:absolute;top:7.77%;left:3.31%;width:48.34%;height:40.28%'>
<div class=O style='position:absolute;top:52.82%;left:55.23%;width:35.76%;
height:37.98%'><span style='position:absolute;top:0%;left:0%;width:94.81%'><span
style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%;
color:#E9EB5D'><i> the </i></span></span><span style='position:absolute;
top:16.74%;left:0%;width:95.92%'><span style='font-size:233%;color:#E9EB5D'><i>Ctbp2
</i></span><span style='font-size:233%;color:#E9EB5D'>cis QTL, </span></span><span
style='position:absolute;top:33.48%;left:0%;width:98.14%'><span
style='font-size:233%;color:#E9EB5D'>but is detected </span></span><span
style='position:absolute;top:49.76%;left:0%;width:94.81%'><span
style='font-size:233%;color:#E9EB5D'>only in the </span></span><span
style='position:absolute;top:66.51%;left:0%;width:100.0%'><span
style='font-size:233%;color:#E9EB5D'>Rosen striatum </span></span><span
style='position:absolute;top:83.25%;left:0%;width:94.81%'><span
style='font-size:233%;color:#E9EB5D'>data set.</span><span style='font-size:
233%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br>
</i></span></span></div>
<div class=O style='position:absolute;top:16.25%;left:56.02%;width:48.21%;
height:12.72%'><span style='position:absolute;top:0%;left:0%;width:94.78%'><span
style='font-size:233%;color:#E9EB5D'>This is</span><span style='font-size:233%;
color:#E9EB5D'><i> the App QTL </i></span></span><span style='position:absolute;
top:48.61%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'>in
the INIA data set. </span><span style='font-size:233%;color:#E9EB5D;mso-special-format:
lastCR;display:none'><i><br>
</i></span></span></div>
<img border=0 src="slide0010_image084.png" style='position:absolute;top:39.39%;
left:3.31%;width:48.47%;height:53.35%'><img border=0
src="slide0010_image085.png" style='position:absolute;top:10.95%;left:35.36%;
width:.39%;height:72.79%'><img border=0 src="slide0010_image086.png"
style='position:absolute;top:10.95%;left:31.52%;width:.39%;height:72.79%'></div>
<div id=NotesObj style='display:none'>
<table style='color:white' border=0 width="100%">
<tr>
<td width=5 nowrap></td>
<td width="100%"></td>
</tr>
<tr>
<td colspan=1></td>
<td align=left colspan=1><font face=Verdana size=3>This slide illustrates one
reason why Ctbp2 should be considered a high priority positional candidate
gene that may modulate the expression level of App.<span style="mso-spacerun:
yes"> </span>Ctbp2 is a strong cis QTL in some brain regions (here the
data are taken from the striatum).<span style="mso-spacerun: yes">
</span>If Ctbp2 contains variants that modulate its own expression then these
expression differences may produce many downstream effects. Of course, we now
want to know much more about the known biology of Ctbp2. What kind of gene is
it? To begin to answer that question we can use a number of resources listed
in the LINKS page.</font><br>
</td>
</tr>
<tr>
<td colspan=1></td>
<td align=left colspan=1><br>
</td>
</tr>
<tr>
<td colspan=1></td>
<td align=left colspan=1><font face=Verdana size=3>Notes:</font><br>
</td>
</tr>
<tr>
<td colspan=1></td>
<td align=left colspan=1><font face=Arial size=3>1.</font><font face=Verdana
size=3> The App QTL is bimodal. Perhaps there are actually two causal factors
in this region--one close to 123 Mb and the other close to 127 Mb.</font><br>
</td>
</tr>
<tr>
<td colspan=1></td>
<td align=left colspan=1><font face=Verdana size=3>2. The precision of QTL
mapping depends on several factors, including the effect size and
interactions among QTLs modulating a trait, the number of genetic individuals
that are studied, and the distribution of recombinations in the study
population.<span style="mso-spacerun: yes"> </span>In the case above,
the QTL(s) are likely to be confined to the interval from 120 to 132 Mb. The
bootstrap test (yellow bars shown in some of the previous slides) can be
usual for estimating the consistency of QTL peaks.</font><br>
</td>
</tr>
</table>
</div>
<script language=JavaScript><!--
function playList() {
}
//-->
</script>
</body>
</html>
|
