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<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD><TITLE> UNC Agilent G4121A Liver Database (January/06 Freeze) Orig LOWESS/ WebQTL</TITLE>
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<P class="title">UNC Agilent G4121A Liver Database (Jan06 Freeze) Orig LOWESS <A HREF="/webqtl/main.py?FormID=editHtml"><img src="/images/modify.gif" alt="modify this page" border= 0 valign="middle"></A><BR><BR>Accession number: <A HREF="/webqtl/main.py?FormID=sharinginfo&GN_AccessionId=104">GN104</A></P>
<P class="subtitle"> Summary:</P>
<Blockquote>
This data set provides estimates of mRNA expression in livers of 38 adult BXD recombinant inbred mice measured using Agilent G4121A microarray. Data were generated by a consortium of investigators at the University of North Carolina at Chapel Hill (Akira Maki, Daniel Gatti, David Threadgill, and Ivan Rusyn) and at the University of Tennessee Heath Science Center (Lu Lu, Elissa Chesler, Ken Manly, and Rob Williams). Image intensity data were processed using a locally weighted scatterplot smooth (LOWESS) and are presented without further modification (Orig LOWESS; see section below on Data Processing). This is the final data freeze.
</Blockquote>
<P class="subtitle"> About the cases used to generate this set of data:</P>
<Blockquote>Ninety-six BXD liver sample pools were obtained from animals reared at UTHSC in a pathogen-free vivarium. Mice were experimentally naive and housed at weaning (20 to 24 days-of-age) in same-sex groups in standard mouse shoebox cages. Mice were 56 to 177 days old at the time of sacrifice. Forty mouse strains were used of which 37 were represented by both sexes.</Blockquote>
<Blockquote>
<table border="0" cellpadding="0" cellspacing="0" bgcolor="#000000" width="60%" align="Center">
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<td>
<table width="100%" border="0" cellpadding="5" cellspacing="1">
<tr bgcolor="royalblue"><td><font color=#FFFFFF>Strain Name</font></td>
<td><font color=#FFFFFF><center>Sex</center></font></td>
<td><font color=#FFFFFF><center>WebQTL Strain ID</center></font></td></tr>
<tr bgcolor="#eeeeee"><td>C57BL/6J</td><td><center>M and F</center></td><td><center>C57BL/6J<center></td></tr>
<tr bgcolor="#eeeeee"><td>DBA/2J</td><td><center>M and F</center></td><td><center>DBA/2J<center></td></tr>
<tr bgcolor="#eeeeee"><td>B6D2F1</td><td><center>M and F</center></td><td><center>F1<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD1</td><td><center>M and F</center></td><td><center>BXD1<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD2</td><td><center>M and F</center></td><td><center>BXD2<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD5</td><td><center>M and F</center></td><td><center>BXD5<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD6</td><td><center>M and F</center></td><td><center>BXD6<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD8</td><td><center>M and F</center></td><td><center>BXD8<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD9</td><td><center>M and F</center></td><td><center>BXD9<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD11</td><td><center>M and F</center></td><td><center>BXD11<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD12</td><td><center>M and F</center></td><td><center>BXD12<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD13</td><td><center>M and F</center></td><td><center>BXD13<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD14</td><td><center>F</center></td><td><center>BXD14<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD15</td><td><center>M and F</center></td><td><center>BXD15<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD21</td><td><center>M and F</center></td><td><center>BXD21<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD23</td><td><center>M and F</center></td><td><center>BXD23<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD24</td><td><center>M</center></td><td><center>BXD24<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD28</td><td><center>M and F</center></td><td><center>BXD28<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD29</td><td><center>M and F</center></td><td><center>BXD29<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD31</td><td><center>M and F</center></td><td><center>BXD31<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD32</td><td><center>M and F</center></td><td><center>BXD32<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD33</td><td><center>F</center></td><td><center>BXD33<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD34</td><td><center>M and F</center></td><td><center>BXD34<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD38</td><td><center>M and F</center></td><td><center>BXD38<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD39</td><td><center>M and F</center></td><td><center>BXD39<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD40</td><td><center>M and F</center></td><td><center>BXD40<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD42</td><td><center>M and F</center></td><td><center>BXD42<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD43</td><td><center>M and F</center></td><td><center>BXD43<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD44</td><td><center>M and F</center></td><td><center>BXD44<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD45</td><td><center>M and F</center></td><td><center>BXD45<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD48</td><td><center>M and F</center></td><td><center>BXD48<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD51</td><td><center>M and F</center></td><td><center>BXD51<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD60</td><td><center>M and F</center></td><td><center>BXD60<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD62</td><td><center>M and F</center></td><td><center>BXD62<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD69</td><td><center>M and F</center></td><td><center>BXD69<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD73</td><td><center>M and F</center></td><td><center>BXD73<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD77</td><td><center>M and F</center></td><td><center>BXD77<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD85</td><td><center>M and F</center></td><td><center>BXD85<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD86</td><td><center>M and F</center></td><td><center>BXD86<center></td></tr>
<tr bgcolor="#eeeeee"><td>BXD92</td><td><center>M and F</center></td><td><center>BXD92<center></td></tr> </table>
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</Blockquote>
<Blockquote>
C57BL/6J, DBA/2J, and BXD1 through BXD42 were originally obtained from The Jackson Laboratory. Advanced intercross BXD strains (BXD43 and higher) were generated at Princeton University and UTHSC (Peirce and Lu, <A href="http://www.biomedcentral.com/1471-2156/5/7" class="normal" target="_Blank" >2004</A>). All of these new strains were inbred for at least 14 generations.
</Blockquote>
<P class="subtitle"> About the tissue used to generate these data:</P>
<Blockquote><P>Animals were killed by cervical dislocation. The entire liver was removed within less than 5 minutes by Zhiping Jia or Hongtao Zhai and placed in RNAlater (Ambion) overnight at 4 degrees C. Tissue was stored in single vials (2 to 3 cases per vial) at -80 degrees C. Tissue vials were shipped to UNC on ice by FedEX. Prior to isolation of RNA, liver samples from the same strain and sex (2 to 3 animals) were pooled in equal amount and minced in a homogenization buffer using an electric homogenizer. Total RNA was isolated using Qiagen RNeasy Mini kits according to the manufacturer's instructions. RNA purity and quality were verified using a BioAnalyzer 2100 and Low RNA Input Linear Amplification kits (Agilent Technologies, Wilmington, DE) in these experiments. RNA labeling, array hybridization and washing and other procedures were performed according to the manufacturer's protocols. A common reference design was used. Male C57BL/6J mouse pooled (equal amounts of RNA from liver, kidney, lung, brain and spleen) RNA provided by the Toxicogenomics Research Consortium was used as a common reference in all these experiments.
</P></Blockquote>
<P class="subtitle"> About the array platform</P>
<Blockquote>Samples were assayed using <A HREF="http://www.chem.agilent.com/scripts/generic.asp?lPage=10408&indcol=Y&prodcol=Y" target="_Blank" class="normal"> G4121A</A> Agilent oligomer microarray glass slides (<A HREF="http://www.ncbi.nlm.nih.gov/projects/geo/query/acc.cgi?acc=GPL891 target="_Blank" class="normal"> GEO Platform ID GPL891</A>). This microarray estimate expression of approximately 20,842 mouse genes, including a special set of toxicology transcripts nominated by a collaboration that included the National Institute of Environmental Health Sciences (NIEHS) and members of the Toxicogenomics Research Consortium.
</Blockquote>
<Blockquote>
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<tr bgcolor="royalblue"><td><center><font color=#FFFFFF>Experiment ID</font></center></td>
<td><center><font color=#FFFFFF>Experiment Name</font></center></td>
<td><center><font color=#FFFFFF>Batch#</font></center></td>
<td><center><font color=#FFFFFF>Sample ID</font></center></td>
<td><center><font color=#FFFFFF>Strain</font></center></td>
<td><center><font color=#FFFFFF>Sex</font></center></td>
<td><center><font color=#FFFFFF>Array Barcode#</font></center></td>
<td><center><font color=#FFFFFF>Pool Size</font></center></td></tr>
<tr bgcolor=#eeeeee><td><center>35783</center></td><td><center>DBA/2J F B1</center></td><td><center>1</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197818090</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35782</center></td><td><center>C57BL/6J F B1</center></td><td><center>1</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197818089</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35770</center></td><td><center>BXD1 M</center></td><td><center>1</center></td><td><center>9</center></td><td><center>BXD1</center></td><td><center>M</center></td><td><center>251197817936</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35771</center></td><td><center>BXD12 F</center></td><td><center>1</center></td><td><center>13</center></td><td><center>BXD12</center></td><td><center>F</center></td><td><center>251197817937</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35772</center></td><td><center>BXD23 M</center></td><td><center>1</center></td><td><center>24</center></td><td><center>BXD14</center></td><td><center>M</center></td><td><center>251197817938</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35774</center></td><td><center>BXD28 M</center></td><td><center>1</center></td><td><center>29</center></td><td><center>BXD28</center></td><td><center>M</center></td><td><center>251197817940</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35775</center></td><td><center>BXD29 F</center></td><td><center>1</center></td><td><center>30</center></td><td><center>BXD29</center></td><td><center>F</center></td><td><center>251197817941</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35773</center></td><td><center>BXD34 F</center></td><td><center>1</center></td><td><center>43</center></td><td><center>BXD34</center></td><td><center>F</center></td><td><center>251197817939</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35776</center></td><td><center>BXD39 M</center></td><td><center>1</center></td><td><center>54</center></td><td><center>BXD39</center></td><td><center>M</center></td><td><center>251197817942</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35777</center></td><td><center>BXD40 F</center></td><td><center>1</center></td><td><center>59</center></td><td><center>BXD40</center></td><td><center>F</center></td><td><center>251197817943</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35778</center></td><td><center>BXD77 M</center></td><td><center>1</center></td><td><center>500</center></td><td><center>BXD77</center></td><td><center>M</center></td><td><center>251197817959</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35768</center></td><td><center>B6D2F1 M B1</center></td><td><center>1</center></td><td><center>609</center></td><td><center>B6D2F1</center></td><td><center>M</center></td><td><center>251197817934</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35779</center></td><td><center>BXD8 F</center></td><td><center>1</center></td><td><center>613</center></td><td><center>BXD8</center></td><td><center>F</center></td><td><center>251197817960</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35781</center></td><td><center>C57BL/6J M B1</center></td><td><center>1</center></td><td><center>614</center></td><td><center>C57BL/6J</center></td><td><center>M</center></td><td><center>251197818088</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35780</center></td><td><center>C57BL/6J M B1R</center></td><td><center>1</center></td><td><center>614</center></td><td><center>C57BL/6J</center></td><td><center>M</center></td><td><center>251197817961</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35535</center></td><td><center>DBA/2J F B2</center></td><td><center>2</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197818160</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35537</center></td><td><center>DBA/2J F B2(3)</center></td><td><center>2</center></td><td><center>3</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197818162</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35536</center></td><td><center>DBA/2J M B2</center></td><td><center>2</center></td><td><center>4</center></td><td><center>DBA/2J</center></td><td><center>M</center></td><td><center>251197818161</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35941</center></td><td><center>C57BL/6J F</center></td><td><center>2</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197818159</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35509</center></td><td><center>C57BL/6J M B2</center></td><td><center>2</center></td><td><center>8</center></td><td><center>C57BL/6J</center></td><td><center>M</center></td><td><center>251197818158</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35529</center></td><td><center>C57BL/6J M B2R</center></td><td><center>2</center></td><td><center>8</center></td><td><center>C57BL/6J</center></td><td><center>M</center></td><td><center>251197818086</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35540</center></td><td><center>BXD11 M</center></td><td><center>2</center></td><td><center>12</center></td><td><center>BXD11</center></td><td><center>M</center></td><td><center>251197818178</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35541</center></td><td><center>BXD13 F</center></td><td><center>2</center></td><td><center>16</center></td><td><center>BXD13</center></td><td><center>F</center></td><td><center>251197818179</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35542</center></td><td><center>BXD21 M</center></td><td><center>2</center></td><td><center>21</center></td><td><center>BXD21</center></td><td><center>M</center></td><td><center>251197818180</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35514</center></td><td><center>BXD31 M</center></td><td><center>2</center></td><td><center>34</center></td><td><center>BXD31</center></td><td><center>M</center></td><td><center>251197817944</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35515</center></td><td><center>BXD32 F</center></td><td><center>2</center></td><td><center>36</center></td><td><center>BXD32</center></td><td><center>F</center></td><td><center>251197817945</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35516</center></td><td><center>BXD42 M</center></td><td><center>2</center></td><td><center>63</center></td><td><center>BXD42</center></td><td><center>M</center></td><td><center>251197817946</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35517</center></td><td><center>BXD5 F</center></td><td><center>2</center></td><td><center>65</center></td><td><center>BXD5</center></td><td><center>F</center></td><td><center>251197817947</center></td><td><center>2</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35527</center></td><td><center>BXD62 M</center></td><td><center>2</center></td><td><center>79</center></td><td><center>BXD62</center></td><td><center>M</center></td><td><center>251197817948</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35531</center></td><td><center>BXD43 F</center></td><td><center>2</center></td><td><center>81</center></td><td><center>BXD43</center></td><td><center>F</center></td><td><center>251197818085</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35539</center></td><td><center>B6D2F1 F</center></td><td><center>2</center></td><td><center>601</center></td><td><center>B6D2F1</center></td><td><center>F</center></td><td><center>251197818177</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35528</center></td><td><center>B6D2F1 M</center></td><td><center>2</center></td><td><center>603</center></td><td><center>B6D2F1</center></td><td><center>M</center></td><td><center>251197818084</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35919</center></td><td><center>DBA/2J F B3</center></td><td><center>3</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197817968</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35918</center></td><td><center>C57BL/6J F B3</center></td><td><center>3</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197817967</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35923</center></td><td><center>BXD14 M</center></td><td><center>3</center></td><td><center>17</center></td><td><center>BXD14</center></td><td><center>M</center></td><td><center>251197817972</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35924</center></td><td><center>BXD15 F</center></td><td><center>3</center></td><td><center>19</center></td><td><center>BXD15</center></td><td><center>F</center></td><td><center>251197817973</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35938</center></td><td><center>BXD36M</center></td><td><center>3</center></td><td><center>46</center></td><td><center>BXD36</center></td><td><center>M</center></td><td><center>16011978011758</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35937</center></td><td><center>BXD38F</center></td><td><center>3</center></td><td><center>52</center></td><td><center>BXD38</center></td><td><center>F</center></td><td><center>16011978011757</center></td><td><center>3</center></td></tr>
<tr bgcolor=#eeeeee><td><center>35939</center></td><td><center>BXD6 M</center></td><td><center>3</center></td><td><center>69</center></td><td><center>BXD6</center></td><td><center>M</center></td><td><center>16011978011759</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35936</center></td><td><center>BXD9F</center></td><td><center>3</center></td><td><center>70</center></td><td><center>BXD9</center></td><td><center>F</center></td><td><center>16011978011756</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35930</center></td><td><center>BXD60 M</center></td><td><center>3</center></td><td><center>86</center></td><td><center>BXD60</center></td><td><center>M</center></td><td><center>16011978011860</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35931</center></td><td><center>BXD44 F</center></td><td><center>3</center></td><td><center>87</center></td><td><center>BXD44</center></td><td><center>F</center></td><td><center>16011978011861</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35916</center></td><td><center>C57BL/6J M B3</center></td><td><center>3</center></td><td><center>506</center></td><td><center>C57BL/6J</center></td><td><center>M</center></td><td><center>251197817964</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35932</center></td><td><center>C57BL/6J M B3R</center></td><td><center>3</center></td><td><center>506</center></td><td><center>C57BL/6J</center></td><td><center>M</center></td><td><center>16011978011862</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35917</center></td><td><center>DBA/2J M B3</center></td><td><center>3</center></td><td><center>509</center></td><td><center>DBA/2J</center></td><td><center>M</center></td><td><center>251197817966</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35921</center></td><td><center>B6D2F1 F B3</center></td><td><center>3</center></td><td><center>602</center></td><td><center>B6D2F1</center></td><td><center>F</center></td><td><center>251197817970</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35920</center></td><td><center>C57BL/6J F B3(605)</center></td><td><center>3</center></td><td><center>605</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197817969</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35922</center></td><td><center>B6D2F1 M B3</center></td><td><center>3</center></td><td><center>702</center></td><td><center>B6D2F1</center></td><td><center>M</center></td><td><center>251197817971</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39357</center></td><td><center>B4R DBA/2J F</center></td><td><center>4R</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197826041</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39356</center></td><td><center>B4R C57BL/6J F B4</center></td><td><center>4R</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197826040</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39383</center></td><td><center>B4R C57BL/6J F B4R</center></td><td><center>4R</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197826108</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39352</center></td><td><center>B4R BXD24 F</center></td><td><center>4R</center></td><td><center>25</center></td><td><center>BXD24</center></td><td><center>M</center></td><td><center>251197826026</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39355</center></td><td><center>B4R BXD40 M</center></td><td><center>4R</center></td><td><center>60</center></td><td><center>BXD40</center></td><td><center>M</center></td><td><center>251197826029</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39361</center></td><td><center>B4R BXD51 M</center></td><td><center>4R</center></td><td><center>77</center></td><td><center>BXD51</center></td><td><center>M</center></td><td><center>251197826105</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39360</center></td><td><center>B4R DBA/2J F (607)</center></td><td><center>4R</center></td><td><center>607</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197826043</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39354</center></td><td><center>B4R BXD32 M</center></td><td><center>4R</center></td><td><center>701</center></td><td><center>BXD32</center></td><td><center>M</center></td><td><center>251197826028</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39351</center></td><td><center>B4R BXD23 F</center></td><td><center>4R</center></td><td><center>704</center></td><td><center>BXD14</center></td><td><center>F</center></td><td><center>251197826025</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39359</center></td><td><center>B4R BXD16 M</center></td><td><center>4R</center></td><td><center>803</center></td><td><center>BXD16</center></td><td><center>M</center></td><td><center>251197826044</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39353</center></td><td><center>B4R BXD19 F</center></td><td><center>4R</center></td><td><center>804</center></td><td><center>BXD19</center></td><td><center>F</center></td><td><center>251197826027</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39381</center></td><td><center>B4R BXD62 F</center></td><td><center>4R</center></td><td><center>812</center></td><td><center>BXD62</center></td><td><center>F</center></td><td><center>251197826106</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39348</center></td><td><center>B4R BXD69 F</center></td><td><center>4R</center></td><td><center>813</center></td><td><center>BXD69</center></td><td><center>F</center></td><td><center>251197825672</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39349</center></td><td><center>B4R BXD73 M</center></td><td><center>4R</center></td><td><center>816</center></td><td><center>BXD73</center></td><td><center>M</center></td><td><center>251197825673</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39347</center></td><td><center>B4R BXD8 M</center></td><td><center>4R</center></td><td><center>817</center></td><td><center>BXD8</center></td><td><center>M</center></td><td><center>251197825670</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39382</center></td><td><center>B4R BXD85 F</center></td><td><center>4R</center></td><td><center>818</center></td><td><center>BXD85</center></td><td><center>F</center></td><td><center>251197826107</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39346</center></td><td><center>B4R BXD86 F</center></td><td><center>4R</center></td><td><center>819</center></td><td><center>BXD86</center></td><td><center>F</center></td><td><center>251197825669</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39350</center></td><td><center>B4R BXD92 F</center></td><td><center>4R</center></td><td><center>821</center></td><td><center>BXD92</center></td><td><center>F</center></td><td><center>251197825674</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>39358</center></td><td><center>B4R C57BL/6J F B4(823)</center></td><td><center>4R</center></td><td><center>823</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197826042</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35550</center></td><td><center>DBA/2J F B5</center></td><td><center>5</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197817950</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35549</center></td><td><center>C57BL/6J F B5</center></td><td><center>5</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197817949</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35587</center></td><td><center>C57BL/6J F B5R</center></td><td><center>5</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197818022</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35558</center></td><td><center>BXD1 F</center></td><td><center>5</center></td><td><center>11</center></td><td><center>BXD1</center></td><td><center>F</center></td><td><center>251197818036</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35551</center></td><td><center>BXD12 M</center></td><td><center>5</center></td><td><center>14</center></td><td><center>BXD12</center></td><td><center>M</center></td><td><center>251197817952</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35552</center></td><td><center>BXD13 M</center></td><td><center>5</center></td><td><center>15</center></td><td><center>BXD13</center></td><td><center>M</center></td><td><center>251197817953</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35584</center></td><td><center>BXD15 M</center></td><td><center>5</center></td><td><center>18</center></td><td><center>BXD15</center></td><td><center>M</center></td><td><center>251197818034</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35557</center></td><td><center>BXD28 F</center></td><td><center>5</center></td><td><center>28</center></td><td><center>BXD28</center></td><td><center>F</center></td><td><center>251197818035</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35553</center></td><td><center>BXD60 F</center></td><td><center>5</center></td><td><center>84</center></td><td><center>BXD60</center></td><td><center>F</center></td><td><center>251197818019</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35585</center></td><td><center>BXD77 F</center></td><td><center>5</center></td><td><center>499</center></td><td><center>BXD77</center></td><td><center>F</center></td><td><center>251197818037</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35555</center></td><td><center>BXD45 F</center></td><td><center>5</center></td><td><center>515</center></td><td><center>BXD45</center></td><td><center>F</center></td><td><center>251197818021</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35787</center></td><td><center>DBA/2J F B6</center></td><td><center>6</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197818005</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35786</center></td><td><center>C57BL/6J F B6</center></td><td><center>6</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197818004</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35800</center></td><td><center>C57BL/6J F B6R</center></td><td><center>6</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197818123</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35795</center></td><td><center>BXD21 F</center></td><td><center>6</center></td><td><center>20</center></td><td><center>BXD21</center></td><td><center>F</center></td><td><center>251197818114</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35791</center></td><td><center>BXD24 M</center></td><td><center>6</center></td><td><center>26</center></td><td><center>BXD24</center></td><td><center>M</center></td><td><center>251197818115</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35794</center></td><td><center>BXD31 F</center></td><td><center>6</center></td><td><center>32</center></td><td><center>BXD31</center></td><td><center>F</center></td><td><center>251197818118</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35792</center></td><td><center>BXD33 F</center></td><td><center>6</center></td><td><center>40</center></td><td><center>BXD33</center></td><td><center>F</center></td><td><center>251197818116</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35790</center></td><td><center>BXD34 M</center></td><td><center>6</center></td><td><center>42</center></td><td><center>BXD34</center></td><td><center>M</center></td><td><center>251197818008</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35793</center></td><td><center>BXD9 M</center></td><td><center>6</center></td><td><center>71</center></td><td><center>BXD9</center></td><td><center>M</center></td><td><center>251197818117</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35797</center></td><td><center>BXD44 M</center></td><td><center>6</center></td><td><center>502</center></td><td><center>BXD44</center></td><td><center>M</center></td><td><center>251197818120</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35788</center></td><td><center>C57BL/6J M B6(507)</center></td><td><center>6</center></td><td><center>507</center></td><td><center>C57BL/6J</center></td><td><center>M</center></td><td><center>251197818006</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35789</center></td><td><center>DBA/2J M B6(510)</center></td><td><center>6</center></td><td><center>510</center></td><td><center>DBA/2J</center></td><td><center>M</center></td><td><center>251197818007</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35798</center></td><td><center>BXD48 M</center></td><td><center>6</center></td><td><center>512</center></td><td><center>BXD48</center></td><td><center>M</center></td><td><center>251197818121</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35796</center></td><td><center>BXD2 F</center></td><td><center>6</center></td><td><center>612</center></td><td><center>BXD2</center></td><td><center>F</center></td><td><center>251197818119</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35567</center></td><td><center>DBA/2J F B7</center></td><td><center>7</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197818069</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35566</center></td><td><center>C57BL/6J F B7</center></td><td><center>7</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197818023</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35577</center></td><td><center>C57BL/6J F B7R</center></td><td><center>7</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197818156</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35568</center></td><td><center>BXD29 M</center></td><td><center>7</center></td><td><center>31</center></td><td><center>BXD29</center></td><td><center>M</center></td><td><center>251197818071</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35570</center></td><td><center>BXD38 M</center></td><td><center>7</center></td><td><center>51</center></td><td><center>BXD38</center></td><td><center>M</center></td><td><center>251197818073</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35573</center></td><td><center>BXD42 F</center></td><td><center>7</center></td><td><center>62</center></td><td><center>BXD42</center></td><td><center>F</center></td><td><center>251197818126</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35574</center></td><td><center>BXD5 M</center></td><td><center>7</center></td><td><center>66</center></td><td><center>BXD5</center></td><td><center>M</center></td><td><center>251197818127</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35940</center></td><td><center>BXD6 F</center></td><td><center>7</center></td><td><center>68</center></td><td><center>BXD6</center></td><td><center>F</center></td><td><center>251197818128</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35508</center></td><td><center>BXD43 M</center></td><td><center>7</center></td><td><center>82</center></td><td><center>BXD43</center></td><td><center>M</center></td><td><center>251197818157</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>35575</center></td><td><center>DBA/2J M B7</center></td><td><center>7</center></td><td><center>511</center></td><td><center>DBA/2J</center></td><td><center>M</center></td><td><center>251197818070</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38670</center></td><td><center>DBA/2J F B8-02 (1)</center></td><td><center>8</center></td><td><center>1</center></td><td><center>DBA/2J</center></td><td><center>F</center></td><td><center>251197828123</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38669</center></td><td><center>C57BL/6J F </center></td><td><center>8</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197828122</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38668</center></td><td><center>C57BL/6J F </center></td><td><center>8</center></td><td><center>5</center></td><td><center>C57BL/6J</center></td><td><center>F</center></td><td><center>251197828099</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38697</center></td><td><center>BXD36 F R</center></td><td><center>8</center></td><td><center>48</center></td><td><center>BXD36</center></td><td><center>F</center></td><td><center>251197828218</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38698</center></td><td><center>BXD39 F R</center></td><td><center>8</center></td><td><center>56</center></td><td><center>BXD39</center></td><td><center>F</center></td><td><center>251197828219</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38661</center></td><td><center>BXD48 F</center></td><td><center>8</center></td><td><center>92</center></td><td><center>BXD48</center></td><td><center>F</center></td><td><center>251197828096</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38673</center></td><td><center>BXD14 F</center></td><td><center>8</center></td><td><center>610</center></td><td><center>BXD23</center></td><td><center>F</center></td><td><center>251197828126</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38686</center></td><td><center>BXD2 M</center></td><td><center>8</center></td><td><center>611</center></td><td><center>BXD2</center></td><td><center>M</center></td><td><center>251197828134</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38672</center></td><td><center>BXD11/TY F</center></td><td><center>8</center></td><td><center>703</center></td><td><center>BXD11/TY</center></td><td><center>F</center></td><td><center>251197828125</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38671</center></td><td><center>B6D2F1 F</center></td><td><center>8</center></td><td><center>801</center></td><td><center>B6D2F1</center></td><td><center>F</center></td><td><center>251197828124</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38694</center></td><td><center>BXD16 F</center></td><td><center>8</center></td><td><center>802</center></td><td><center>BXD16</center></td><td><center>F</center></td><td><center>251197828215</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38696</center></td><td><center>BXD19 M</center></td><td><center>8</center></td><td><center>805</center></td><td><center>BXD19</center></td><td><center>M</center></td><td><center>251197828217</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38695</center></td><td><center>BXD33 F</center></td><td><center>8</center></td><td><center>809</center></td><td><center>BXD33</center></td><td><center>F</center></td><td><center>251197828216</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38667</center></td><td><center>BXD45 M</center></td><td><center>8</center></td><td><center>811</center></td><td><center>BXD45</center></td><td><center>M</center></td><td><center>251197828098</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38687</center></td><td><center>BXD69 M</center></td><td><center>8</center></td><td><center>814</center></td><td><center>BXD69</center></td><td><center>M</center></td><td><center>251197828135</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38688</center></td><td><center>BXD73 F</center></td><td><center>8</center></td><td><center>815</center></td><td><center>BXD73</center></td><td><center>F</center></td><td><center>251197828136</center></td><td><center>2</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38689</center></td><td><center>BXD92 M</center></td><td><center>8</center></td><td><center>822</center></td><td><center>BXD92</center></td><td><center>M</center></td><td><center>251197828146</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38685</center></td><td><center>BXD86 M</center></td><td><center>8</center></td><td><center>901</center></td><td><center>BXD86</center></td><td><center>M</center></td><td><center>251197828133</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38660</center></td><td><center>BXD51 F</center></td><td><center>8</center></td><td><center>902</center></td><td><center>BXD51</center></td><td><center>F</center></td><td><center>251197828092</center></td><td><center>3</center></td></tr>
<tr bgcolor="#eeeeee"><td><center>38666</center></td><td><center>BXD85 M</center></td><td><center>8</center></td><td><center>903</center></td><td><center>BXD85</center></td><td><center>M</center></td><td><center>251197828097</center></td><td><center>3</center></td></tr>
</table>
</center></td>
</tr>
</table>
</Blockquote>
<Blockquote>
<P>The strains of pooled samples were verified by Daniel Gatti and Rob Williams by comparing the genotype on the arrays with the known genotype for each strain. Sample 17 (BXD14M) did not match any other strain. This sample was removed from the dataset. Sample 610 (BXD14F) was found to match the BXD23F genotype and was reassigned. Samples 24 & 704 (BXD23M & F) matched the BXD14 genotype and were reassigned as BXD14 mice. Samples 46 & 48 were found to be of mixed genotype and were removed from the dataset. </P>
<P>The sexes of each pooled sample were checked by Daniel Gatti using Y chromosome expression. Sample 25 (BXD24F) was found to be male. This change was made and there are two BXD24 male samples. Samples 40 & 809 (BXD33) were both found to be female.</P>
</Blockquote>
<P class="subtitle"> About data processing:</P>
<Blockquote>Expression data were initially expressed as the ratio of the liver fluorescence signal to that generated by the reference mRNA sample (liver, kidney, lung, brain, and spleen) for each probe. Data were normalized using a robust <A HREF="http://cm.bell-labs.com/cm/ms/departments/sia/wsc/smoothsoft.html" target="_blank" class="normal">LOWESS</A> smoothing method that adjusts for non-linearity of signal in the two channels. We then computed the log base 2 of these ratios (median). A value of -1 indicates that expression in liver is roughly 1/2 that in the control; a value of -2 indicates that expression in the liver is roughly 1/4 that in the control, etc. Conversely, a value of +2 indicates that the expression in liver is 4-fold greater in liver.
</Blockquote>
<!-- Download the entire original data <a href="./dbdoc/Liver_95_UNCMD.xls" class="normal">file</a> -->
<P class="subtitle"> About the chromosome and megabase position values:</P>
<Blockquote>The chromosomal locations of probes were determined by <A target="_blank" href="http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi?CMD=Web&LAYOUT=TwoWindows&AUTO_FORMAT=Semiauto&ALIGNMENTS=50&ALIGNMENT_VIEW=Tabular&CLIENT=web&DATABASE=nr&DESCRIPTIONS=100&ENTREZ_QUERY=%28none%29&EXPECT=10&FILTER=L&FORMAT_OBJECT=Alignment&FORMAT_TYPE=HTML&NCBI_GI=on&PAGE=MegaBlast&SERVICE=plain&SET_DEFAULTS.x=34&SET_DEFAULTS.y=8&SHOW_OVERVIEW=on&END_OF_HTTPGET=Yes&SHOW_LINKOUT=yes&GET_SEQUENCE=yes&PERC_IDENT=None,1,-2" class="normal">NCBI's megablast</A> using the NCBI M32 genomic sequence. Gene markers were determined by BLAT analysis using the Mouse Genome Sequencing Consortium May 2004 Assembly (see <A href="http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&org=mouse" target="_blank" class="normal">http://genome.ucsc.edu/</A> ). We thank Kenneth Phillips (Paradigm Genetics, Inc) for helping generate the probes position data set.
</Blockquote>
<P class="subtitle"> Data source acknowledgment:</P>
<Blockquote>This project was supported by ES10126, ES11391, ES11660 and P20-MH 62009 to KFM and RW. Ivan Rusyn was a recipient of a Transition to Independent Position Award (ES11660) from the National Institute of Environmental Health Sciences. Work by Paradigm Genetics, Inc. in design of the Toxicogenomics Micro (G4121A) array was supported by NIEHS contract N01-ES-25497.</blockquote>
<Blockquote>Please contact either:</Blockquote>
<Blockquote><A HREF="http://www.sph.unc.edu/search/profile.cfm?id=2510" target="_blank" class="normal">Ivan Rusyn</A> at the Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7431, USA or </Blockquote>
<Blockquote><A target="_blank" HREF="http://www.nervenet.org/people/rob_cv.html" class="normal">Rob Williams</A> at the Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 855 Monroe Avenue, Memphis, TN 38163, USA </Blockquote>
</Blockquote>
<P class="subtitle"> About this text file:</P>
<Blockquote>
This text file originally generated by Ivan Rusyn, David W. Threadgill and Robert W. Williams, July 2004. Updated by RWW, Nov 14, 16, 2004. Updated by IR, Dec 1, 2004. Updated by DMG, Jan. 5, 2006.
</Blockquote>
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