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Diffstat (limited to 'general/news')
| -rw-r--r-- | general/news/news.md | 4 |
1 files changed, 2 insertions, 2 deletions
diff --git a/general/news/news.md b/general/news/news.md index e52d2ad..49a0222 100644 --- a/general/news/news.md +++ b/general/news/news.md @@ -15,9 +15,9 @@ This evaluation of genotypes is based on data extracted from whole-genome sequencing of BXD male spleen samples obtained in January 2017 from the Jackson Laboratory whenever possible, and from the UTHSC colony in all other cases. All animals were born between January and December 2016. -1. BXD65b (sourced from JAX). The sequence-based genotypes correlate better to array-based 2017 genotypes of BXD65 and BXD65a than with the BXD65b array-based genotypes. It is possible that there are two somewhat distinct sub-strains now called BXD65b—one at JAX and one at UTHSC. **Recommended Action:**Sourcing BXD65b animals from JAX. +- 1. BXD65b (sourced from JAX). The sequence-based genotypes correlate better to array-based 2017 genotypes of BXD65 and BXD65a than with the BXD65b array-based genotypes. It is possible that there are two somewhat distinct sub-strains now called BXD65b—one at JAX and one at UTHSC. **Recommended Action:**Sourcing BXD65b animals from JAX. -2. BXD53 array-based genotypes do not correlate well with sequence-based genotypes for BXD53/2. BXD53 was rederived a second time by JAX and is now referred to formally at BXD53/2RwwJ (Stock 017749). The likely reason for the discrepancy is that we sequenced BXD53/2RwwJ (Stock 017749) rather than the older BXD53 (sourced from UTHSC) for array-based genotyping. BXD53 sequence-based genotypes correlate well with eQTL data for BXD53. **Recommended Action:**If using BXD53/2RwwJ, please obtain current sequence-based genotypes from David Ashbrook. +- 2. BXD53 array-based genotypes do not correlate well with sequence-based genotypes for BXD53/2. BXD53 was rederived a second time by JAX and is now referred to formally at BXD53/2RwwJ (Stock 017749). The likely reason for the discrepancy is that we sequenced BXD53/2RwwJ (Stock 017749) rather than the older BXD53 (sourced from UTHSC) for array-based genotyping. BXD53 sequence-based genotypes correlate well with eQTL data for BXD53. **Recommended Action:**If using BXD53/2RwwJ, please obtain current sequence-based genotypes from David Ashbrook. 3. BXD68 animals at UTHSC used from about 2012 to 2017 were BXD34 animals. Genotypes in the 2017 array-based file HAVE or HAVE NOT been corrected. We have corrected this error in some phenotype data sets. BXD68 from The Jackson Laboratory are genuine BXD68. We sequenced BXD68 DNA from JAX. **Recommended Action: **None unless you have used BXD68 animals obtained from UTHSC between 2012 and 2017. If so, these animals should be relabeled BXD34. |