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+<p><strong>Cases</strong>. A total of 58 strains, including 54 BXD strains, both parental strains (C57BL/6J and DBA/2J) and both reciprocal F1 hybrids (B6D2F1 and D2B6F1) were analyzed. In most cases, two arrays were processed per strain--one for males and one for females. All tissue and RNA was extracted by K. Mozhui. Samples were pooled by sex and usually include at least two cases per sex and strain.</p>
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+<p><strong>Sex Balance</strong>. Fifty strains have matched male and female samples. Five strains have male only samples (BXD5, 13, 16, 100 and 103). Three strains have only female samples (BXD44, 70, and 87.) Please review the expression data for <em>Xist</em> probe set 10606178. This non-coding RNA is expressed highly only in females and can be used to check the sex of a sample or pool of tissue. <em>Ddx3y</em> probe set 10608138 can also be used. This is a Y chromosome gene that is expressed abundantly in male samples and at background levels in female samples.</p>