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Diffstat (limited to 'general/datasets/G2NEI_ILM_Retina_BXD_RI0410/experiment-design.rtf')
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diff --git a/general/datasets/G2NEI_ILM_Retina_BXD_RI0410/experiment-design.rtf b/general/datasets/G2NEI_ILM_Retina_BXD_RI0410/experiment-design.rtf deleted file mode 100644 index 4fff707..0000000 --- a/general/datasets/G2NEI_ILM_Retina_BXD_RI0410/experiment-design.rtf +++ /dev/null @@ -1,12 +0,0 @@ -<p>Expression profiling by array</p>
-
-<p>We used pooled RNA samples of retinas, usually two independent pools--two male, two female pool--for most lines of mice.</p>
-
-<p>All normalization was performed by William E. Orr in the HEI Vision Core Facility</p>
-
-<ol>
- <li>Computed the log base 2 of each raw signal value</li>
- <li>Calculated the mean and standard Deviation of each Mouse WG-6 v2.0 array</li>
- <li>Normalized each array using the formula, 2 (z-score of log2 [intensity]) The result is to produce arrays that have a mean of 8, a variance of 4, and a standard deviation of 2. The advantage is that a two-fold difference in expression level corresponds approximately to a 1 unit difference.</li>
- <li>computed the mean of the values for the set of microarrays for each strain. Technical replicates were averaged before computing the mean for independent biological samples.</li>
-</ol>
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