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authorBonface2024-02-13 23:52:26 -0600
committerMunyoki Kilyungi2024-08-09 13:30:43 +0300
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tree3dd2883524985114070a7770cd2e9f9bd7eb1848 /general/datasets/Inia_hyp_pca_0813/experiment-design.rtf
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downloadgn-docs-b2feda451ccfbeaed02dce9088d6dd228cf15861.tar.gz
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+<p>Hypothalamus was dissected from adult male and female mice and process for expression analysis.</p>
+
+<p>RNA isolation<br />
+Total RNA was purified using the RNAeasy micro kit on the QIAcube system (www.qiagen.com). RNA purity and concentration was checked using 260/280 nm absorbance ratio and RNA integrity was analyzed using the Agilent Bioanalyzer 2100 (Agilent Technologies).</p>
+
+<p>This preliminary data set is associated with 430 eQTLs with LOD scores above 10. Peak LRS is 146 for Trait ID 10513604 (<em>Hdhd3</em>).</p>