<blockquote>
<p>Parental and 31 BXD lines were studied. Mice were received from Jackson Laboratory, or from The Oak Ridge National and were bred in the facility of the Neuro-BSIK consortium (VU University Amsterdam). The data set includes expression values for 18 of the BXD strains made by Benjamin Taylor at the Jackson Laboratory in the 1970s and 1990s (BXD1 through BXD40, as well as the two parental strains, C57BL/6J and DBA/2J. All of these strains are fully inbred, many well beyond the 100th filial (F) generation of inbreeding.</p>
<p>BXD spleen sample pools (from 2-3 mice) were obtained from a pathogen-free mice of the Dutch Mouse Phenomics Consortium (MPC) in Amsterdam. Mice were imported into the central animal facility at the HZI and kept in a pathogen-free vivarium. Mice were euthanized using CO2 and spleenocytes wre prepared. Most mice were between 17 and 22 weeks of age when samples were collected. FACS sorting was used to select the CD4-positive T cells. These cells were further separated into CD4+CD25+ and CD4+CD25- pools.</p>
<p>Error-checking strain identity. A set of more than 20 probe sets with Mendelian segregation patterns in this HZI data set were used to confirm strain identify in early June, 2007. Two errors were detected and rectified. As of June 22, 2007, data are registered correctly. Prior to June 22, 2007, data listed as strains BXD33 and BXD39 were essentially hybrid (mixed) data sets.</p>
<p>On Aug 23, 2007, we loaded the final QTL Reaper data into GeneNetwork for the corrected data set. The maximum LRS generated by any probe set is 84.6 for 1436240_at (<em>Tra2a</em>). A total of 41 probe sets are associated with QTLs that have LRS values above 46 (LOD > 10).</p>
<p> Table 1</p>
<blockquote>
<table border="1" cellpadding="0" cellspacing="0" style="width:600px">
<tbody>
<tr>
<td>
<table align="left" border="1" cellpadding="5" cellspacing="1" style="width:600px">
<tbody>
<tr>
<td>Index</td>
<td>ProbeSet ID</td>
<td>Sample Description</td>
<td>Sex</td>
<td>Strain</td>
<td>cd25</td>
<td>Microarray</td>
<td>Short Description</td>
<td>Age</td>
<td>Pool No.</td>
<td>Pool members (animal number)</td>
<td>Date of preparation</td>
</tr>
<tr>
<td>1</td>
<td>HZI1176</td>
<td>BXD-06f (f1) CD25</td>
<td>F</td>
<td>BXD6</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-06f</td>
<td>17</td>
<td>f1</td>
<td>1,3,4</td>
<td>1-31-2006</td>
</tr>
<tr>
<td>2</td>
<td>HZI1177</td>
<td>BXD-06m (m2) CD25</td>
<td>M</td>
<td>BXD6</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-06m</td>
<td>18</td>
<td>m2</td>
<td>5,6,7</td>
<td>1-31-2006</td>
</tr>
<tr>
<td>3</td>
<td>HZI1178</td>
<td>BXD-14m (m3) CD25</td>
<td>M</td>
<td>BXD14</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-14m</td>
<td>17</td>
<td>m3</td>
<td>1,3,4</td>
<td>1-31-2006</td>
</tr>
<tr>
<td>4</td>
<td>HZI1179</td>
<td>BXD-34f (f4) CD25</td>
<td>F</td>
<td>BXD34</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-34f</td>
<td>17</td>
<td>f4</td>
<td>1,2,3</td>
<td>2-1-2006</td>
</tr>
<tr>
<td>5</td>
<td>HZI1180</td>
<td>BXD-34m (m5) CD25</td>
<td>M</td>
<td>BXD34</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-34m</td>
<td>17</td>
<td>m5</td>
<td>5,7,8</td>
<td>2-1-2006</td>
</tr>
<tr>
<td>6</td>
<td>HZI1181</td>
<td>BXD-40f (f6) CD25</td>
<td>F</td>
<td>BXD40</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-40f</td>
<td>17</td>
<td>f6</td>
<td>1,2,3</td>
<td>2-1-2006</td>
</tr>
<tr>
<td>7</td>
<td>HZI1182</td>
<td>BXD-40m (m7) CD25</td>
<td>M</td>
<td>BXD40</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-40m</td>
<td>17</td>
<td>m7</td>
<td>5,6,7</td>
<td>2-2-2006</td>
</tr>
<tr>
<td>8</td>
<td>HZI1183</td>
<td>BXD-02f (f8) CD25</td>
<td>F</td>
<td>BXD2</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-02f</td>
<td>17</td>
<td>f8</td>
<td>1,2,3</td>
<td>2-14-2006</td>
</tr>
<tr>
<td>9</td>
<td>HZI1184</td>
<td>BXD-02m (m20) CD25</td>
<td>M</td>
<td>BXD2</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-02m</td>
<td>21</td>
<td>m20</td>
<td>4,5,6</td>
<td>4-6-2006</td>
</tr>
<tr>
<td>10</td>
<td>HZI1185</td>
<td>BXD-11f (f30) CD25</td>
<td>F</td>
<td>BXD11</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-11f</td>
<td>17</td>
<td>f30</td>
<td>3,4,5</td>
<td>5-11-2006</td>
</tr>
<tr>
<td>11</td>
<td>HZI1186</td>
<td>BXD-11m (m9) CD25</td>
<td>M</td>
<td>BXD11</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-11m</td>
<td>18</td>
<td>m9</td>
<td>1,2</td>
<td>2-14-2006</td>
</tr>
<tr>
<td>12</td>
<td>HZI1187</td>
<td>BXD-12f (f10) CD25</td>
<td>F</td>
<td>BXD12</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-12f</td>
<td>17</td>
<td>f10</td>
<td>1,2,3</td>
<td>2-14-2006</td>
</tr>
<tr>
<td>13</td>
<td>HZI1188</td>
<td>BXD-39f (f23) CD25</td>
<td>F</td>
<td>BXD39</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-39f</td>
<td>19</td>
<td>f23</td>
<td>4,5,6</td>
<td>4-11-2006</td>
</tr>
<tr>
<td>14</td>
<td>HZI1191</td>
<td>BXD-18m (m13) CD25</td>
<td>M</td>
<td>BXD18</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-18m</td>
<td>18</td>
<td>m13</td>
<td>7,8</td>
<td>2-15-2006</td>
</tr>
<tr>
<td>15</td>
<td>HZI1192</td>
<td>BXD-23m (m15) CD25</td>
<td>M</td>
<td>BXD23</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-23m</td>
<td>18</td>
<td>m15</td>
<td>1,2,3</td>
<td>2-15-2006</td>
</tr>
<tr>
<td>16</td>
<td>HZI1194</td>
<td>BXD-09f (f17) CD25</td>
<td>F</td>
<td>BXD9</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-09f</td>
<td>21</td>
<td>f17</td>
<td>1,2,3</td>
<td>4-5-2006</td>
</tr>
<tr>
<td>17</td>
<td>HZI1195</td>
<td>BXD-09m (m16) CD25</td>
<td>M</td>
<td>BXD9</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-09m2</td>
<td>21</td>
<td>m16</td>
<td>5,6</td>
<td>4-5-2006</td>
</tr>
<tr>
<td>18</td>
<td>HZI1196</td>
<td>BXD-09m (m35) CD25</td>
<td>M</td>
<td>BXD9</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-09m</td>
<td>15</td>
<td>m35</td>
<td>7,8,9</td>
<td>7-7-2006</td>
</tr>
<tr>
<td>19</td>
<td>HZI1197</td>
<td>BXD-32f (f18) CD25</td>
<td>F</td>
<td>BXD32</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-32f</td>
<td>21</td>
<td>f18</td>
<td>1,2,3</td>
<td>4-6-2006</td>
</tr>
<tr>
<td>20</td>
<td>HZI1198</td>
<td>BXD-32m (m19) CD25</td>
<td>M</td>
<td>BXD32</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-32m</td>
<td>22</td>
<td>m19</td>
<td>1,2,3</td>
<td>4-6-2006</td>
</tr>
<tr>
<td>21</td>
<td>HZI1199</td>
<td>BXD-33f (f22) CD25</td>
<td>F</td>
<td>BXD33</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-33f</td>
<td>18</td>
<td>f22</td>
<td>2,3,4</td>
<td>4-11-2006</td>
</tr>
<tr>
<td>22</td>
<td>HZI1200</td>
<td>BXD-39m (m29) CD25</td>
<td>M</td>
<td>BXD39</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-39m</td>
<td>17</td>
<td>m29</td>
<td>5,6,7</td>
<td>5-10-2006</td>
</tr>
<tr>
<td>23</td>
<td>HZI1201</td>
<td>BXD-01f (f32) CD25</td>
<td>F</td>
<td>BXD1</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-01f</td>
<td>18</td>
<td>f32</td>
<td>3,4</td>
<td>7-6-2006</td>
</tr>
<tr>
<td>24</td>
<td>HZI1202</td>
<td>BXD-01m (m31) CD25</td>
<td>M</td>
<td>BXD1</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-01m</td>
<td>18</td>
<td>m31</td>
<td>1,2</td>
<td>7-6-2006</td>
</tr>
<tr>
<td>25</td>
<td>HZI1203</td>
<td>BXD-16f (f26) CD25</td>
<td>F</td>
<td>BXD16</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-16f</td>
<td>18</td>
<td>f26</td>
<td>1,2,3</td>
<td>4-12-2006</td>
</tr>
<tr>
<td>26</td>
<td>HZI1204</td>
<td>BXD-21f (f25) CD25</td>
<td>F</td>
<td>BXD21</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-21f</td>
<td>19</td>
<td>f25</td>
<td>5,6,7</td>
<td>4-12-2006</td>
</tr>
<tr>
<td>27</td>
<td>HZI1205</td>
<td>BXD-21m (m24) CD25</td>
<td>M</td>
<td>BXD21</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-21m</td>
<td>18</td>
<td>m24</td>
<td>1,2,3</td>
<td>4-12-2006</td>
</tr>
<tr>
<td>28</td>
<td>HZI1208</td>
<td>C57BL/6Jf (f28) CD25</td>
<td>F</td>
<td>C57BL/6J</td>
<td>CD25-</td>
<td>Yes</td>
<td>C57BL/6Jf</td>
<td>16</td>
<td>f28</td>
<td>1,2,3</td>
<td>5-10-2006</td>
</tr>
<tr>
<td>29</td>
<td>HZI1209</td>
<td>DBA/2Jf (f27) CD25</td>
<td>F</td>
<td>DBA/2J</td>
<td>CD25-</td>
<td>Yes</td>
<td>DBA/2Jf</td>
<td>16</td>
<td>f27</td>
<td>5,6,7</td>
<td>5-10-2006</td>
</tr>
<tr>
<td>30</td>
<td>HZI1210</td>
<td>DBA/2Jm (m21) CD25</td>
<td>M</td>
<td>DBA/2J</td>
<td>CD25-</td>
<td>Yes</td>
<td>DBA/2Jm</td>
<td>21</td>
<td>m21</td>
<td>1,2,3</td>
<td>4-11-2006</td>
</tr>
<tr>
<td>31</td>
<td>HZI2473</td>
<td>BXD-13 m 45</td>
<td>M</td>
<td>BXD13</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-13m</td>
<td>15</td>
<td>m45</td>
<td>4,5,6,7</td>
<td>12-13-2006</td>
</tr>
<tr>
<td>32</td>
<td>HZI2474</td>
<td>BXD-19 m 46</td>
<td>M</td>
<td>BXD19</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-19m</td>
<td>16</td>
<td>m46</td>
<td>4,5,6</td>
<td>12-15-2006</td>
</tr>
<tr>
<td>33</td>
<td>HZI2475</td>
<td>BXD-28 m 43</td>
<td>M</td>
<td>BXD28</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-28m</td>
<td>17,2</td>
<td>m43</td>
<td>1,2,3</td>
<td>10-23-2006</td>
</tr>
<tr>
<td>34</td>
<td>HZI2476</td>
<td>BXD-29 m 37</td>
<td>M</td>
<td>BXD29</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-29m</td>
<td>20, 16</td>
<td>m37</td>
<td>1,2,3</td>
<td>8-29-2006</td>
</tr>
<tr>
<td>35</td>
<td>HZI2477</td>
<td>BXD-31 m 69</td>
<td>M</td>
<td>BXD31</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-31m</td>
<td>14</td>
<td>m69</td>
<td>4,5,6</td>
<td>2-1-2008</td>
</tr>
<tr>
<td>36</td>
<td>HZI2478</td>
<td>BXD-33 m 11</td>
<td>M</td>
<td>BXD33</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-33m</td>
<td>17</td>
<td>m11</td>
<td>1,2</td>
<td>2-14-2006</td>
</tr>
<tr>
<td>37</td>
<td>HZI2479</td>
<td>BXD-38 m 63</td>
<td>M</td>
<td>BXD38</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-38m</td>
<td>18</td>
<td>m63</td>
<td>1,2,3</td>
<td>6-20-2007</td>
</tr>
<tr>
<td>38</td>
<td>HZI2480</td>
<td>BXD-42 m 47</td>
<td>M</td>
<td>BXD42</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-42m</td>
<td>15,16</td>
<td>m47</td>
<td>1,2,3</td>
<td>12-15-2006</td>
</tr>
<tr>
<td>39</td>
<td>HZI2481</td>
<td>BXD-42 m 65</td>
<td>M</td>
<td>BXD42</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-42m</td>
<td>15,16</td>
<td>m47</td>
<td>1,2,3</td>
<td>12-15-2006</td>
</tr>
<tr>
<td>40</td>
<td>HZI2482</td>
<td>BXD-13 f 44</td>
<td>F</td>
<td>BXD13</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-13f</td>
<td>15</td>
<td>f44</td>
<td>1,2,3</td>
<td>12-13-2006</td>
</tr>
<tr>
<td>41</td>
<td>HZI2483</td>
<td>BXD-18 F 14</td>
<td>F</td>
<td>BXD18</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-18f</td>
<td>17</td>
<td>f14</td>
<td>3,4,5</td>
<td>2-15-2006</td>
</tr>
<tr>
<td>42</td>
<td>HZI2484</td>
<td>BXD-19 f 38</td>
<td>F</td>
<td>BXD19</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-19f2</td>
<td>21</td>
<td>f38</td>
<td>1,2,3</td>
<td>9-1-2006</td>
</tr>
<tr>
<td>43</td>
<td>HZI2485</td>
<td>BXD-19 f 64</td>
<td>F</td>
<td>BXD19</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-19f</td>
<td>19</td>
<td>f64</td>
<td>7,8,9</td>
<td>6-20-2007</td>
</tr>
<tr>
<td>44</td>
<td>HZI2486</td>
<td>BXD-28 f 61</td>
<td>F</td>
<td>BXD28</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-28f</td>
<td>22</td>
<td>f61</td>
<td>1,2,3</td>
<td>6-18-2007</td>
</tr>
<tr>
<td>45</td>
<td>HZI2487</td>
<td>BXD-29 f 40</td>
<td>F</td>
<td>BXD29</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-29f</td>
<td>15 - 16</td>
<td>f40</td>
<td>4,5,6</td>
<td>9-25-2006</td>
</tr>
<tr>
<td>46</td>
<td>HZI2488</td>
<td>BXD-31 f 34</td>
<td>F</td>
<td>BXD31</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-31f</td>
<td>16</td>
<td>f34</td>
<td>1,2,3</td>
<td>7-7-2006</td>
</tr>
<tr>
<td>47</td>
<td>HZI2489</td>
<td>BXD-38 f 70</td>
<td>F</td>
<td>BXD38</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-38f</td>
<td>13</td>
<td>f70</td>
<td>4,5,6,7</td>
<td>2-1-2008</td>
</tr>
<tr>
<td>48</td>
<td>HZI2490</td>
<td>BXD-42 f 49</td>
<td>F</td>
<td>BXD42</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-42f</td>
<td>17</td>
<td>f49</td>
<td>??</td>
<td>3-8-2007</td>
</tr>
<tr>
<td>49</td>
<td>HZI2491</td>
<td>BXD-43 f 53</td>
<td>F</td>
<td>BXD43</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-43f</td>
<td>16</td>
<td>f53</td>
<td>1,2,3</td>
<td>4-23-2007</td>
</tr>
<tr>
<td>50</td>
<td>HZI2492</td>
<td>BXD-44 f 54</td>
<td>F</td>
<td>BXD44</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-44f</td>
<td>18</td>
<td>f54</td>
<td>1,2,3</td>
<td>4-23-2007</td>
</tr>
<tr>
<td>51</td>
<td>HZI2493</td>
<td>BXD-45 f 55</td>
<td>F</td>
<td>BXD45</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-45f</td>
<td>19</td>
<td>f55</td>
<td>1,2,3</td>
<td>4-23-2007</td>
</tr>
<tr>
<td>52</td>
<td>HZI2494</td>
<td>BXD-51 f 59</td>
<td>F</td>
<td>BXD51</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-51f</td>
<td>22</td>
<td>f59</td>
<td>1,2,3</td>
<td>6-18-2007</td>
</tr>
<tr>
<td>53</td>
<td>HZI2495</td>
<td>BXD-62 f 56</td>
<td>F</td>
<td>BXD62</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-62f</td>
<td>17</td>
<td>f56</td>
<td>1,2,3</td>
<td>4-26-2007</td>
</tr>
<tr>
<td>54</td>
<td>HZI2496</td>
<td>BXD-73 f 57</td>
<td>F</td>
<td>BXD73</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-73f</td>
<td>18</td>
<td>f57</td>
<td>1,2,3</td>
<td>4-26-2007</td>
</tr>
<tr>
<td>55</td>
<td>HZI2497</td>
<td>BXD-75 f 58</td>
<td>F</td>
<td>BXD75</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-75f</td>
<td>15,17</td>
<td>f58</td>
<td>1,2,3</td>
<td>4-26-2007</td>
</tr>
<tr>
<td>56</td>
<td>HZI2498</td>
<td>BXD-86 f 52</td>
<td>F</td>
<td>BXD86</td>
<td>CD25-</td>
<td>Yes</td>
<td>BXD-86f</td>
<td>16</td>
<td>f52</td>
<td>1,2,3</td>
<td>4-18-2007</td>
</tr>
</tbody>
</table>
</td>
</tr>
</tbody>
</table>
</blockquote>
</blockquote>
