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-rw-r--r--scripts/calculate_biweight.R43
-rw-r--r--scripts/wgcna_analysis.R17
2 files changed, 15 insertions, 45 deletions
diff --git a/scripts/calculate_biweight.R b/scripts/calculate_biweight.R
deleted file mode 100644
index 8d8366e..0000000
--- a/scripts/calculate_biweight.R
+++ /dev/null
@@ -1,43 +0,0 @@
-
-library(testthat)
-library(WGCNA)
-
-arg_values <- commandArgs(trailingOnly = TRUE)
-ParseArgs <- function(args){
-
- trait_vals <- as.numeric(unlist(strsplit(args[1], split=" ")))
- target_vals <- as.numeric(unlist(strsplit(args[2], split=" ")))
-
- return(list(trait_vals= c(trait_vals),target_vals = c(target_vals)))
-
-}
-BiweightMidCorrelation <- function(trait_val,target_val){
-
- results <-bicorAndPvalue(as.numeric(unlist(trait_val)),as.numeric(unlist(target_val)))
- return ((c(c(results$bicor)[1],c(results$p)[1])))
-
-}
-
-
-
-test_that("biweight results"),{
- vec_1 <- c(1,2,3,4)
- vec_2 <- c(1,2,3,4)
-
- results <- BiweightMidCorrelation(vec_1,vec_2)
- expect_equal(c(1.0,0.0),results)
-}
-
-
-test_that("parsing args "),{
- my_args <- c("1 2 3 4","5 6 7 8")
- results <- ParseArgs(my_args)
-
- expect_equal(results[1],c(1,2,3,4))
- expect_equal(results[2],c(5,6,7,8))
-}
-
-parsed_values <- ParseArgs(arg_values)
-
-
-cat(BiweightMidCorrelation(parsed_values[1],parsed_values[2])) \ No newline at end of file
diff --git a/scripts/wgcna_analysis.R b/scripts/wgcna_analysis.R
index 17b3537..b0d25a9 100644
--- a/scripts/wgcna_analysis.R
+++ b/scripts/wgcna_analysis.R
@@ -6,11 +6,13 @@ library(rjson)
options(stringsAsFactors = FALSE);
-imgDir = Sys.getenv("GENERATED_IMAGE_DIR")
+cat("Running the wgcna analysis script\n")
+
# load expression data **assumes from json files row(traits)(columns info+samples)
# pass the file_path as arg
# pass the file path to read json data
+
args = commandArgs(trailingOnly=TRUE)
if (length(args)==0) {
@@ -21,6 +23,7 @@ if (length(args)==0) {
}
inputData <- fromJSON(file = json_file_path)
+imgDir = inputData$TMPDIR
trait_sample_data <- do.call(rbind, inputData$trait_sample_data)
@@ -83,6 +86,11 @@ network <- blockwiseModules(dataExpr,
+cat("Generated network \n")
+
+network
+
+
genImageRandStr <- function(prefix){
randStr <- paste(prefix,stri_rand_strings(1, 9, pattern = "[A-Za-z0-9]"),sep="_")
@@ -90,14 +98,19 @@ genImageRandStr <- function(prefix){
return(paste(randStr,".png",sep=""))
}
+
mergedColors <- labels2colors(network$colors)
imageLoc <- file.path(imgDir,genImageRandStr("WGCNAoutput"))
png(imageLoc,width=1000,height=600,type='cairo-png')
+
+cat("Generating the CLuster dendrogram\n")
+
+
plotDendroAndColors(network$dendrograms[[1]],mergedColors[network$blockGenes[[1]]],
"Module colors",
-dendroLabels = FALSE, hang = 0.03,
+dendroLabels = NULL, hang = 0.03,
addGuide = TRUE, guideHang = 0.05)