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diff --git a/web/tutorial/ppt/WebQTLDemo_files/master02.htm b/web/tutorial/ppt/WebQTLDemo_files/master02.htm
deleted file mode 100755
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--- a/web/tutorial/ppt/WebQTLDemo_files/master02.htm
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-<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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- else
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-
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-
- if( fEnable )
- document.write( '</a>' );
- if ( drawing_app ) {
- document.write( ' &nbsp &nbsp &nbsp &nbsp<a href=\"javascript:parent.GoToLast()\">' );
- document.write( '<img src="next_active.gif"></a>' );
- }
-
-
- document.write( '</td> </tr></table>' );
-
- if ( ! drawing_app && !false ) {
- document.write( '</td> <td align=right valign=top>' );
- document.write( '<a href=\"javascript:parent.FullScreen()\"> ' +
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-</script></center> </body> </html> \ No newline at end of file
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> <meta name=Robots content=NoIndex> <style> <!--a {text-decoration: none;}--> </style> </head> <body bgcolor=black text=white link=white vlink=white alink=white onload=" parent.base.highlite();" onresize="self.location.reload();"> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID1 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p1" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('1')" id=AREF>WebQTL Demonstration One<br> please link to <br> www.webqtl.org/search.html</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID2 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p2" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('2')" id=AREF>&quot;or webqtl.org/search.html...&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID3 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p3" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('3')" id=AREF>Search results</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID4 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p4" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('4')" id=AREF>&quot;First page of data:&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID5 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p5" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('5')" id=AREF>&quot;Data sources:&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID6 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p6" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('6')" id=AREF>&quot;Return to Trait Data page&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID7 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p7" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('7')" id=AREF>&quot;Discovering shared expression patterns&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID8 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p8" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('8')" id=AREF>&quot;The App transcript neighborhood&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID9 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p9" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('9')" id=AREF>&quot;Handdrawn sketch of the neighborhood&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID10 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p10" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('10')" id=AREF>&quot;What a network is likely...&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID11 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p11" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('11')" id=AREF>Are there experimental results to corroborate a link between App with Hsp84-1?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID12 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p12" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('12')" id=AREF>&quot;2.45 billion scatter plots&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID13 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p13" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('13')" id=AREF>&quot;Cross-tissue type correlations&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID14 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p14" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('14')" id=AREF>&quot;Cross-modal correlations:&quot;</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID15 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p15" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('15')" id=AREF>WebQTL<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp; </span><br> link to <br> www.webqtl.org/search.html</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID16 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p16" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('16')" id=AREF>Slide 16</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID17 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p17" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('17')" id=AREF>Slide 17</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID18 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p18" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('18')" id=AREF>WebQTL to exploring upstream control</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID19 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p19" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('19')" id=AREF>WebQTL to exploring upstream control.</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID20 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p20" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('20')" id=AREF>The whole neighborhood is modulated!</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID21 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p21" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('21')" id=AREF>Which gene is the QTL?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID22 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p22" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('22')" id=AREF>Slide 22</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID23 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p23" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('23')" id=AREF>Slide 23</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID24 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p24" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('24')" id=AREF>Tissue differences in probe performance</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID25 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p25" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('25')" id=AREF>Is there known biology to link Hars2 with App?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID26 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p26" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('26')" id=AREF>WebQTL<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp; </span><br> link to <br> www.webqtl.org/search.html</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID27 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p27" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('27')" id=AREF>Requirement: The gene must be polymorphic to be genetically ÒupstreamÓ</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID28 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p28" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('28')" id=AREF>Direct correlations between genotypes and traits</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID29 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p29" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('29')" id=AREF>WhatÕs downstream of Chr 2 near Hars2?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID30 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p30" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('30')" id=AREF>WhatÕs downstream of Chr 2 near Hars2?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID31 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p31" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('31')" id=AREF>Does Hars2 correlate with Actn2 strongly?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID32 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p32" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('32')" id=AREF>Contact for comments and improvements:</a></b></font></td> </tr> </table> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/WebQTLDemo_files/outline_expand.gif b/web/tutorial/ppt/WebQTLDemo_files/outline_expand.gif
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/outline_expanded.htm b/web/tutorial/ppt/WebQTLDemo_files/outline_expanded.htm
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@@ -1,5 +0,0 @@
-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> <meta name=Robots content=NoIndex> <style> <!--a {text-decoration: none;}--> </style> </head> <body bgcolor=black text=white link=white vlink=white alink=white onload=" parent.base.highlite();" onresize="self.location.reload();"> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID1 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p1" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('1')" id=AREF>WebQTL Demonstration One<br> please link to <br> www.webqtl.org/search.html</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Part 1: How to discover shared expression patterns (slides 2Ð14)</font></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Part 2. Discovering upstream modulators (15Ð25)</font></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering downstream targets</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID2 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p2" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('2')" id=AREF>&quot;or webqtl.org/search.html...&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>or webqtl.org/search.html (mirror)</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID3 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p3" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('3')" id=AREF>Search results</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID4 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p4" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('4')" id=AREF>&quot;First page of data:&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>First page of data: The ÒTrait Data FormÓ</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID5 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p5" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('5')" id=AREF>&quot;Data sources:&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Data sources: Phenotpyes and genotypes</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID6 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p6" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('6')" id=AREF>&quot;Return to Trait Data page&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Return to Trait Data page</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID7 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p7" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('7')" id=AREF>&quot;Discovering shared expression patterns&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering shared expression patterns</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID8 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p8" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('8')" id=AREF>&quot;The App transcript neighborhood&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>The App transcript neighborhood</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID9 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p9" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('9')" id=AREF>&quot;Handdrawn sketch of the neighborhood&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Handdrawn sketch of the neighborhood</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID10 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p10" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('10')" id=AREF>&quot;What a network is likely...&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>What a network is likely to look like (but App will not be center of universe).</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID11 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p11" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('11')" id=AREF>Are there experimental results to corroborate a link between App with Hsp84-1?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID12 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p12" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('12')" id=AREF>&quot;2.45 billion scatter plots&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>2.45 billion scatter plots: here is one of the best</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID13 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p13" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('13')" id=AREF>&quot;Cross-tissue type correlations&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Cross-tissue type correlations</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID14 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p14" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('14')" id=AREF>&quot;Cross-modal correlations:&quot;</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Cross-modal correlations: From mRNA to to anatomy and to behavior and pharmacology</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID15 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p15" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('15')" id=AREF>WebQTL<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp; </span><br> link to <br> www.webqtl.org/search.html</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering shared expression patterns</font></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering upstream modulators (QTLs)</font></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering downstream targets</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID16 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p16" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('16')" id=AREF>Slide 16</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID17 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p17" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('17')" id=AREF>Slide 17</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID18 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p18" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('18')" id=AREF>WebQTL to exploring upstream control</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID19 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p19" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('19')" id=AREF>WebQTL to exploring upstream control.</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID20 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p20" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('20')" id=AREF>The whole neighborhood is modulated!</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID21 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p21" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('21')" id=AREF>Which gene is the QTL?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID22 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p22" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('22')" id=AREF>Slide 22</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID23 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p23" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('23')" id=AREF>Slide 23</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID24 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p24" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('24')" id=AREF>Tissue differences in probe performance</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID25 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p25" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('25')" id=AREF>Is there known biology to link Hars2 with App?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID26 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p26" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('26')" id=AREF>WebQTL<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp; </span><br> link to <br> www.webqtl.org/search.html</a></b></font></td> </tr> </table> <table> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering shared expression patterns</font></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering upstream modulators (QTLs)</font></td> </tr> <tr> <td colspan=1></td> <td colspan=1><font face=Arial size=2>Discovering downstream targets</font></td> </tr> </table> <br> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID27 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p27" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('27')" id=AREF>Requirement: The gene must be polymorphic to be genetically ÒupstreamÓ</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID28 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p28" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('28')" id=AREF>Direct correlations between genotypes and traits</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID29 VISIBILITY=hidden >&gt;</ilayer>'); } </script></td> <td width=100% id="p29" ><font face=Arial size=2><b><a href="javascript:parent.base.Slide('29')" id=AREF>WhatÕs downstream of Chr 2 near Hars2?</a></b></font></td> </tr> </table> <table width=100%> <tr> <td width=1 ><script> if ( parent.base.isnav ) { document.write('<ilayer id= LAYERID30 VISIBILITY=hidden >&gt;</ilayer>'); 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- if( IsNts() || (parent.IsFullScrMode() && parent.HideMenu() ) ) return;
- if( ( g_allowAdvOnClick && parent.IsFullScrMode() ) || g_doAdvOnClick ||
- (event && ( (event.keyCode==32) || (event.keyCode == 13) ) ) )
- parent.M_GoNextSld();
-}
-
-
-var g_supportsPPTHTML = SupportsPPTHTML(), g_scaleInFrame = true, gId="", g_bgSound="",
- g_scaleHyperlinks = false, g_allowAdvOnClick = true, g_showInBrowser = false, g_doAdvOnClick = false;
-
- var g_showAnimation = 0;
-var g_hasTrans = false, g_autoTrans = false, g_transSecs = 0;
-var g_animManager = null;
-
-var ENDSHOW_MESG="End of slide show, click to exit.", SCREEN_MODE="Frames", gIsEndShow=0, NUM_VIS_SLDS=32, SCRIPT_HREF="script.js", FULLSCR_HREF="fullscreen.htm";
-var gCurSld = gPrevSld = 1, g_offset = 0, gNtsOpen = gHasNts = gOtlTxtExp = gNarrationPaused = false, gOtlOpen = true
-window.gPPTHTML=SupportsPPTHTML()
-var g_hideNav = 0;
-function UpdNtsPane(){ PPTNts.location.replace( MHTMLPrefix+GetHrefObj( gCurSld ).mNtsHref ) }
-function UpdNavPane( sldIndex ){ if(gNavLoaded) PPTNav.UpdNav() }
-function UpdOtNavPane(){ if(gOtlNavLoaded) PPTOtlNav.UpdOtlNav() }
-function UpdOtlPane(){ if(gOtlLoaded) PPTOtl.UpdOtl() }
-function SetHasNts( fVal )
-{
- if( gHasNts != fVal ) {
- gHasNts=fVal
- UpdNavPane()
- }
-}
-
-function ToggleVNarration()
-{
- if ( base.msie < 0 ) {
- PPTSld.ToggleSound( false, PPTSld.document.NSPlay );
- return;
- }
-
- rObj=PPTSld.document.all("NSPlay")
- if( rObj ) {
- if( gNarrationPaused )
- rObj.Play()
- else
- rObj.Pause()
-
- gNarrationPaused=!gNarrationPaused
- }
-}
-
-function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) }
-function HasPrevSld() { return ( gIsEndShow || ( g_currentSlide != 1 && GetHrefObj( g_currentSlide-1 ).mVis == 1 )||( GetCurrentSlideNum() > 1 ) ) }
-function HasNextSld() { return (GetCurrentSlideNum() != GetNumSlides()) }
-function StartEndShow()
-{
-// g_hideNav = 1;
-// PPTNav.location.reload();
- if( PPTSld.event ) PPTSld.event.cancelBubble=true
-
- doc=PPTSld.document
- doc.open()
- doc.writeln('<html><head><script > /*defer>*/ g_ctxmenu = 0; ' +
- 'if( parent.base.msie < 0 ) { document.captureEvents(Event.KEYPRESS); document.captureEvents(Event.MOUSEDOWN); document.onkeypress = _KPH; document.onmousedown = _KPH; } ' +
- 'function DocumentOnClick(event) { return _KPH(event); } function IsContextMenu() { return (g_ctxmenu ==1); } ' +
- 'function _KPH(event)' +
- '{ ' +
- 'if ( parent.base.msie < 0 && (parent.IsFullScrMode() ) && event ) { if ( (!parent.IsMac() && event.which == 3) || ( parent.IsMac() && (event.modifiers & Event.CONTROL_MASK) && event.which == 1 ) ) { return parent.KPH(event); } ' +
- ' else if (event.which == 27 || event.which == 32 || event.which == 13 || parent.HitOK( event ) || (event.which == 78) || (event.which == 110) || (event.which == 29) || (event.which == 31) || (event.which == 12) ) { if ( IsContextMenu() ) { return parent.KPH(event); } parent.base.CloseFullScreen(); return; } ' +
- ' else if ( (event.which == 80) || (event.which == 112) || (event.which == 30) || (event.which == 28) || (event.which == 11) || (event.which == 8) ) { parent.M_GoPrevSld(); } } ' +
- 'if( parent.HideMenu() ) return; if( (parent.IsFullScrMode() ) && event) { if ( (event.keyCode==27 || event.keyCode == 13 || event.keyCode==32 || event.type=="click" ) || (event.keyCode == 78) || (event.keyCode == 110) ) { parent.base.CloseFullScreen(); }' +
- ' else if ( (event.keyCode == 80) || (event.keyCode == 112) ) { parent.M_GoPrevSld(); } } } function Unload() { parent.gIsEndShow=0; } function SetupEndShow() { if ( !parent.IsFullScrMode() ) { return; } else { parent.PPTNav.location.reload(); } parent.gIsEndShow=1; if ( parent.g_docTable[0].type != "jpeg" ) { if ( parent.base.msie < 0 ) {parent.createCM(); } document.oncontextmenu=parent._CM; } }</script></head><body scroll=\"no\" onclick=\"DocumentOnClick(event)\" onkeypress=\"_KPH(event)\" bgcolor=\"black\" onload=\"SetupEndShow()\" onunload=\"Unload()\"><center><p><font face=Tahoma color=white size=2><br><b>' + ENDSHOW_MESG + '</b></font></p></center></body></html>')
- doc.close()
-}
-function SetSldVisited(){ gDocTable[gCurSld-1].mVisited=true }
-function IsSldVisited(){ return gDocTable[gCurSld-1].mVisited }
-function hrefList( sldHref, visible, sldIdx )
-{
- this.mSldHref= this.mNtsHref = sldHref
- this.mSldIdx = sldIdx
- this.mOrigVis= this.mVis = visible
- this.mVisited= false
-}
-var gDocTable = new Array(
- new hrefList("slide0001.htm", 1, 1),
- new hrefList("slide0002.htm", 1, 2),
- new hrefList("slide0003.htm", 1, 3),
- new hrefList("slide0004.htm", 1, 4),
- new hrefList("slide0005.htm", 1, 5),
- new hrefList("slide0006.htm", 1, 6),
- new hrefList("slide0007.htm", 1, 7),
- new hrefList("slide0008.htm", 1, 8),
- new hrefList("slide0009.htm", 1, 9),
- new hrefList("slide0010.htm", 1, 10),
- new hrefList("slide0011.htm", 1, 11),
- new hrefList("slide0012.htm", 1, 12),
- new hrefList("slide0013.htm", 1, 13),
- new hrefList("slide0014.htm", 1, 14),
- new hrefList("slide0015.htm", 1, 15),
- new hrefList("slide0016.htm", 1, 16),
- new hrefList("slide0017.htm", 1, 17),
- new hrefList("slide0018.htm", 1, 18),
- new hrefList("slide0019.htm", 1, 19),
- new hrefList("slide0020.htm", 1, 20),
- new hrefList("slide0021.htm", 1, 21),
- new hrefList("slide0022.htm", 1, 22),
- new hrefList("slide0023.htm", 1, 23),
- new hrefList("slide0024.htm", 1, 24),
- new hrefList("slide0025.htm", 1, 25),
- new hrefList("slide0026.htm", 1, 26),
- new hrefList("slide0027.htm", 1, 27),
- new hrefList("slide0028.htm", 1, 28),
- new hrefList("slide0029.htm", 1, 29),
- new hrefList("slide0030.htm", 1, 30),
- new hrefList("slide0031.htm", 1, 31),
- new hrefList("slide0032.htm", 1, 32)
-);
-
-function ImgBtn( oId,bId,w,action )
-{
- var t=this
- t.Perform = _IBP
- t.SetActive = _IBSetA
- t.SetInactive= _IBSetI
- t.SetPressed = _IBSetP
- t.SetDisabled= _IBSetD
- t.Enabled = _IBSetE
- t.ChangeIcon = null
- t.UserAction = action
- t.ChgState = _IBUI
- t.mObjId = oId
- t.mBorderId= bId
- t.mWidth = w
- t.mIsOn = t.mCurState = 0
-}
-function _IBSetA()
-{
- if( this.mIsOn ) {
- obj=this.ChgState( gHiliteClr,gShadowClr,2 )
- obj.style.posTop=0
- }
-}
-function _IBSetI()
-{
- if( this.mIsOn ) {
- obj=this.ChgState( gFaceClr,gFaceClr,1 )
- obj.style.posTop=0
- }
-}
-function _IBSetP()
-{
- if( this.mIsOn ) {
- obj=this.ChgState( gShadowClr,gHiliteClr,2 )
- obj.style.posLeft+=1; obj.style.posTop+=1
- }
-}
-function _IBSetD()
-{
- obj=this.ChgState( gFaceClr,gFaceClr,0 )
- obj.style.posTop=0
-}
-function _IBSetE( state )
-{
- var t=this
- GetObj( t.mBorderId ).style.visibility="visible"
- if( state != t.mIsOn ) {
- t.mIsOn=state
- if( state )
- t.SetInactive()
- else
- t.SetDisabled()
- }
-}
-function _IBP()
-{
- var t=this
- if( t.mIsOn ) {
- if( t.UserAction != null )
- t.UserAction()
- if( t.ChangeIcon ) {
- obj=GetObj(t.mObjId)
- if( t.ChangeIcon() )
- obj.style.posLeft=obj.style.posLeft+(t.mCurState-4)*t.mWidth
- else
- obj.style.posLeft=obj.style.posLeft+(t.mCurState-0)*t.mWidth
- }
- t.SetActive()
- }
-}
-function _IBUI( clr1,clr2,nextState )
-{
- var t=this
- SetBorder( GetObj( t.mBorderId ),clr1,clr2 )
- obj=GetObj( t.mObjId )
- obj.style.posLeft=obj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj.style.posTop
- t.mCurState=nextState
- return obj
-}
-function TxtBtn( oId,oeId,action,chkState )
-{
- var t=this
- t.Perform = _TBP
- t.SetActive = _TBSetA
- t.SetInactive= _TBSetI
- t.SetPressed = _TBSetP
- t.SetDisabled= _TBSetD
- t.SetEnabled = _TBSetE
- t.GetState = chkState
- t.UserAction = action
- t.ChgState = _TBUI
- t.mObjId = oId
- t.m_elementsId= oeId
- t.mIsOn = 1
-}
-function _TBSetA()
-{
- var t=this
- if( t.mIsOn && !t.GetState() )
- t.ChgState( gHiliteClr,gShadowClr,0,0 )
-}
-function _TBSetI()
-{
- var t=this
- if( t.mIsOn && !t.GetState() )
- t.ChgState( gFaceClr,gFaceClr,0,0 )
-}
-function _TBSetP()
-{
- if( this.mIsOn )
- this.ChgState( gShadowClr,gHiliteClr,1,1 )
-}
-function _TBSetD()
-{
- this.ChgState( gFaceClr,gFaceClr,0,0 )
- this.mIsOn = 0
-}
-function _TBSetE()
-{
- var t=this
- if( !t.GetState() )
- t.ChgState( gFaceClr,gFaceClr,0,0 )
- else
- t.ChgState( gShadowClr,gHiliteClr,1,1 )
- t.mIsOn = 1
-}
-function _TBP()
-{
- var t=this
- if( t.mIsOn ) {
- if( t.UserAction != null )
- t.UserAction()
- if( t.GetState() )
- t.SetPressed()
- else
- t.SetActive()
- }
-}
-function _TBUI( clr1,clr2,lOffset,tOffset )
-{
- SetBorder( GetObj( this.mObjId ),clr1,clr2 )
- Offset( GetObj( this.m_elementsId ),lOffset,tOffset )
-}
-function GetObj( objId ){ return document.all.item( objId ) }
-function Offset( obj, top, left ){ obj.style.top=top; obj.style.left=left }
-function SetBorder( obj, upperLeft, lowerRight )
-{
- s=obj.style;
- s.borderStyle = "solid"
- s.borderWidth = 1
- s.borderLeftColor = s.borderTopColor = upperLeft
- s.borderBottomColor= s.borderRightColor = lowerRight
-}
-function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] }
-function BtnOnOver(){ b=GetBtnObj(); if( b != null ) b.SetActive() }
-function BtnOnDown(){ b=GetBtnObj(); if( b != null ) b.SetPressed() }
-function BtnOnOut(){ b=GetBtnObj(); if( b != null ) b.SetInactive() }
-function BtnOnUp()
-{
- b=GetBtnObj()
- if( b != null )
- b.Perform()
- else
- Upd()
-}
-function GetNtsState(){ return parent.gNtsOpen }
-function GetOtlState(){ return parent.gOtlOpen }
-function GetOtlTxtState(){ return parent.gOtlTxtExp }
-function NtsBtnSetFlag( fVal )
-{
- s=document.all.item( this.m_flagId ).style
- s.display="none"
- if( fVal )
- s.display=""
- else
- s.display="none"
-}
-
-var gHiliteClr="THREEDHIGHLIGHT",gShadowClr="THREEDSHADOW",gFaceClr="THREEDFACE"
-var gBtnArr = new Array()
-gBtnArr["nb_otl"] = new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPane,GetOtlState )
-gBtnArr["nb_nts"] = new TxtBtn( "nb_nts","nb_ntsElem",parent.ToggleNtsPane,GetNtsState )
-gBtnArr["nb_prev"]= new ImgBtn( "nb_prev","nb_prevBorder",30,parent.GoToPrevSld )
-gBtnArr["nb_next"]= new ImgBtn( "nb_next","nb_nextBorder",30,parent.GoToNextSld )
-gBtnArr["nb_sldshw"]= new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent.FullScreen )
-gBtnArr["nb_voice"] = new ImgBtn( "nb_voice","nb_voiceBorder",18,parent.ToggleVNarration )
-gBtnArr["nb_otlTxt"]= new ImgBtn( "nb_otlTxt","nb_otlTxtBorder",23,parent.ToggleOtlText )
-gBtnArr["nb_nts"].m_flagId= "notes_flag"
-gBtnArr["nb_nts"].SetFlag = NtsBtnSetFlag
-gBtnArr["nb_otlTxt"].ChangeIcon= GetOtlTxtState
-var sNext="Next",sPrev="Previous",sEnd="End Show",sFont="Arial", alwaysOn = false
-function ShowMenu()
-{
- BuildMenu();
- var doc=PPTSld.document.body,x=PPTSld.event.clientX+doc.scrollLeft,y=PPTSld.event.clientY+doc.scrollTop
-
- m = PPTSld.document.all.item("ctxtmenu")
- m.style.pixelLeft=x
- if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) )
- m.style.pixelLeft=x-m.scrollWidth
-
- m.style.pixelTop=y
- if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) )
- m.style.pixelTop=y-m.scrollHeight
-
- m.style.display=""
-}
-function _CM()
-{
- if( !parent.IsFullScrMode() && !alwaysOn) return;
-
- if(!PPTSld.event.ctrlKey) {
- ShowMenu()
- return false
- } else
- HideMenu()
-}
-
-function processNavKPH(event) {
- if ( PPTSld && (event.keyCode != 13 || !event.srcElement.href || event.srcElement.href == "" ) )
- return PPTSld._KPH(event);
-}
-function processNavClick() {
- HideMenu();
- return true;
-}
-function BuildMenu()
-{
- if( PPTSld.document.all.item("ctxtmenu") ) return
-
- var mObj=CreateItem( PPTSld.document.body )
-mObj.id="ctxtmenu"
- var s=mObj.style
- s.position="absolute"
- s.cursor="default"
- s.width="100px"
- SetCMBorder(mObj,"menu","black")
-
- var iObj=CreateItem( mObj )
- SetCMBorder( iObj, "threedhighlight","threedshadow" )
- iObj.style.padding=2
- if ( self.IsFullScrMode() ) {
- CreateMenuItem( iObj,sNext,M_GoNextSld,M_True )
- CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld )
- }
- else {
- CreateMenuItem( iObj,sNext, base.TP_GoToNextSld, base.HasNextSld )
- CreateMenuItem( iObj,sPrev,base.GoToPrevSld, base.HasPrevSld )
- }
- var sObj=CreateItem( iObj )
- SetCMBorder(sObj,"menu","menu")
- var s=sObj.style
- s.borderTopColor="threedshadow"
- s.borderBottomColor="threedhighlight"
- s.height=1
- s.fontSize="0px"
- if ( self.IsFullScrMode() )
- CreateMenuItem( iObj,sEnd,M_End,M_True )
- else
- CreateMenuItem( iObj,sEnd,M_End,M_False )
-}
-function Highlight() { ChangeClr("activecaption","threedhighlight") }
-function Deselect() { ChangeClr("threedface","menutext") }
-function Perform()
-{
- e=PPTSld.event.srcElement
- if( e.type=="menuitem" && e.IsActive() )
- e.Action()
- else
- PPTSld.event.cancelBubble=true
-}
-function ChangeClr( bg,clr )
-{
- e=PPTSld.event.srcElement
- if( e.type=="menuitem" && e.IsActive() ) {
- e.style.backgroundColor=bg
- e.style.color=clr
- }
-}
-
-function M_HasPrevSld() { return( base.HasPrevSld() ) }
-function M_GoNextSld() {
- base.SetFSMode(1);
- if( gIsEndShow )
- M_End();
- else {
- if ( base.HasNextSld() )
- base.GoToNextSld();
- else if ( base.EndSlideShow ) {
- StartEndShow();
- gIsEndShow = 1;
-
- PPTNav.location.reload();
- }
- else
- base.CloseFullScreen();
- }
-}
-function M_GoPrevSld() {
- base.SetFSMode(1);
- g_hideNav = 0;
- if( gIsEndShow ) {
- gIsEndShow = 0;
- if ( base.msie > 0 && IsMac() )
- ChangeFrame( SLIDE_FRAME, GetHrefObj( g_currentSlide ).m_slideHref );
- else
- PPTSld.history.back();
-
- PPTNav.location.reload();
- if( PPTSld.event )
- PPTSld.event.cancelBubble=true;
- }
- else
- base.GoToPrevSld();
-}
-function M_True() { return true }
-function M_False() { return false }
-
-function M_End() {
- base.CloseFullScreen();
- /*PPTSld.event.cancelBubble=true;
- window.close( self )*/
-}
-
-function CreateMenuItem( node,text,action,eval )
-{
- var e=CreateItem( node )
- e.type="menuitem"
- e.Action=action
- e.IsActive=eval
- e.innerHTML=text
-
- if( !e.IsActive() )
- e.style.color="threedshadow"
- e.onclick=Perform
- e.onmouseover=Highlight
- e.onmouseout=Deselect
- s=e.style;
- s.fontFamily=sFont
- s.fontSize="8pt"
- s.paddingLeft=2
-}
-function CreateItem( node )
-{
- var elem=PPTSld.document.createElement("DIV")
- node.insertBefore( elem )
- return elem
-}
-function SetCMBorder( o,ltClr,rbClr )
-{
- var s=o.style
- s.backgroundColor="menu"
- s.borderStyle="solid"
- s.borderWidth=1
- s.borderColor=ltClr+" "+rbClr+" "+rbClr+" "+ltClr
-}
-
-/* netscape context menu */
-g_ctxmenu = 0;
-function setRect( obj, X, Y, W, H ) {
- obj.top = Y;
- obj.left = X;
- obj.clip.top = 0;
- obj.clip.left = 0;
- obj.clip.bottom = H;
- obj.clip.right = W;
-}
-
-function KPH(event) {
- if ( ! base.IsFullScrMode() && !alwaysOn )
- return true;
-
- if ( (!IsMac() &&event.which == 3) || ( IsMac() && (event.modifiers & Event.CONTROL_MASK) && event.which == 1 ) ) {
- PPTSld.g_ctxmenu = 1;
- PPTSld.stripUobj.visibility = "show";
- PPTSld.stripDobj.visibility = "show";
- PPTSld.shadeUobj.visibility = "show";
- PPTSld.shadeDobj.visibility = "show";
- PPTSld.panelobj.visibility = "show";
- PPTSld.Fobj.visibility = "show";
- PPTSld.Bobj.visibility = "show";
- PPTSld.Eobj.visibility = "show";
-
- setRect(PPTSld.shadeUobj, event.pageX-2, event.pageY-2, 82, 67 );
- setRect(PPTSld.shadeDobj, event.pageX, event.pageY, 82, 67 );
- setRect(PPTSld.panelobj, event.pageX, event.pageY, 80, 65 );
- setRect(PPTSld.Fobj, event.pageX, event.pageY, 80, 20 );
- setRect(PPTSld.Bobj, event.pageX, event.pageY+20, 80, 20 );
- setRect(PPTSld.stripUobj, event.pageX, event.pageY+41, 80, 1 );
- setRect(PPTSld.stripDobj, event.pageX, event.pageY+43, 80, 1 );
- setRect(PPTSld.Eobj, event.pageX, event.pageY+45, 80, 20 );
- return false;
- }
- if ( HitOK( event ) ) {
- PPTSld.g_ctxmenu = 0;
- PPTSld.stripUobj.visibility = "hide";
- PPTSld.stripDobj.visibility = "hide";
- PPTSld.shadeUobj.visibility = "hide";
- PPTSld.shadeDobj.visibility = "hide";
- PPTSld.panelobj.visibility = "hide";
- PPTSld.Fobj.visibility = "hide";
- PPTSld.Bobj.visibility = "hide";
- PPTSld.Eobj.visibility = "hide";
- }
- return true;
-}
-
-function overMe() {
- this.bgColor = "blue";
-}
-
-function outMe() {
- this.bgColor = "#AAAAAA";
-}
-
-function makeElement( whichEl, whichContainer ) {
- if ( arguments.length == 1 ) {
- whichContainer = PPTSld;
- }
- tmp = new Layer(100,whichContainer);
- eval( whichEl + " = tmp" );
- return eval(whichEl);
-}
-
-function initMe( obj, clr, text ) {
- obj.bgColor = clr;
-// obj.document.write("<a href='javascript:return false'>" + text + "</a>");
- obj.document.write( "<font size=2 face=Arial " );
- if ( !M_HasPrevSld() && (obj == PPTSld.Bobj ) ) {
- obj.document.write( " color='#808080' " );
- }
- else {
- obj.onmouseover = overMe;
- obj.onmouseout = outMe;
- }
- obj.document.write( " > &nbsp " + text +"</font> <layer top=0 left=0 width=100 height=40 ></layer>");
- obj.document.close();
- obj.captureEvents(Event.CLICK);
- obj.color = "black";
-}
-
-function createCM() {
- if ( base.IsFullScrMode() ) {
- var clr = "#AAAAAA";
- PPTSld.shadeUobj = makeElement("SHADEU");
- PPTSld.shadeDobj = makeElement("SHADED");
- PPTSld.panelobj = makeElement("PANEL");
- PPTSld.stripUobj = makeElement("STRIPU");
- PPTSld.stripDobj = makeElement("STRIPD");
- PPTSld.shadeUobj.bgColor = "#BBBBBB";
- PPTSld.shadeDobj.bgColor = "#888888";
- PPTSld.stripUobj.bgColor = "#777777";
- PPTSld.stripDobj.bgColor = "#CCCCCC";
- PPTSld.panelobj.bgColor = clr;
- PPTSld.Fobj = makeElement("Next");
- PPTSld.Bobj = makeElement("Previous");
- PPTSld.Eobj = makeElement("EndShow");
- initMe( PPTSld.Fobj, clr, "Next" );
- PPTSld.Fobj.onclick = M_GoNextSld;
-
- initMe( PPTSld.Bobj, clr, "Previous" );
- PPTSld.Bobj.onclick = M_GoPrevSld;
-
- initMe( PPTSld.Eobj, clr, "End Show");
- PPTSld.Eobj.onclick = base.CloseFullScreen;
- }
-}
-
-function IsContextMenu() {
- return (g_ctxmenu == 1)
-}
-var g_notesTable = new Array()
-var g_hiddenSlide = new Array()
-makeSlide( 0,1,1);
-makeSlide( 1,1,1);
-makeSlide( 2,1,1);
-makeSlide( 3,1,1);
-makeSlide( 4,1,1);
-makeSlide( 5,1,1);
-makeSlide( 6,1,1);
-makeSlide( 7,1,1);
-makeSlide( 8,1,1);
-makeSlide( 9,1,1);
-makeSlide( 10,1,1);
-makeSlide( 11,1,1);
-makeSlide( 12,1,1);
-makeSlide( 13,1,1);
-makeSlide( 14,1,1);
-makeSlide( 15,1,1);
-makeSlide( 16,1,1);
-makeSlide( 17,1,1);
-makeSlide( 18,1,1);
-makeSlide( 19,1,1);
-makeSlide( 20,1,1);
-makeSlide( 21,1,1);
-makeSlide( 22,1,1);
-makeSlide( 23,1,1);
-makeSlide( 24,1,1);
-makeSlide( 25,1,1);
-makeSlide( 26,1,1);
-makeSlide( 27,1,1);
-makeSlide( 28,1,1);
-makeSlide( 29,1,1);
-makeSlide( 30,1,1);
-makeSlide( 31,1,1);
-
-var END_SHOW_HREF = "endshow.htm",
- OUTLINE_EXPAND_HREF = "outline_expanded.htm",
- OUTLINE_COLLAPSE_HREF = "outline_collapsed.htm",
- OUTLINE_NAVBAR_HREF = "outline_navigation_bar.htm",
- NAVBAR_HREF = "navigation_bar.htm",
- BLANK_NOTES_HREF = "blank_notes.htm",
- NUM_VISIBLE_SLIDES = 32,
- SIMPLE_FRAMESET = 0,
- SLIDE_FRAME = "PPTSld",
- NOTES_FRAME = "PPTNts",
- OUTLINE_FRAME = "PPTOtl",
- OUTLINE_NAVBAR_FRAME = "PPTOtlNav",
- NAVBAR_FRAME = "PPTNav",
- MAIN_FRAME = "MainFrame",
- FS_NAVBAR_HREF = "fs_navigation_bar.htm",
- isIEFiles = 2,
- isNAVFiles = 8,
- isFLATFiles = 16,
- includeNotes = 1,
- PPTPRESENTATION = 1;
-var INITSLIDENUM = 1;
-
-var EndSlideShow = 0;
-var g_outline_href = OUTLINE_COLLAPSE_HREF;
-var g_fullscrMode = 0;
-var FSWin = null;
-var gtmpstr = document.location.href;
-var g_baseURL = gtmpstr.substr(0, gtmpstr.lastIndexOf("/") ) + "/" + "WebQTLDemo_files";
-var g_showoutline = 1;
-var g_shownotes = includeNotes;
-var g_currentSlide = INITSLIDENUM, g_prevSlide = INITSLIDENUM;
-var saveFSSlideNum = saveTPSlideNum = g_currentSlide;
-var saveFSprevSlide = saveTPprevSlide = g_prevSlide;
-var g_slideType="ie";
-var appVer = navigator.appVersion;
-var msie = appVer.indexOf( "MSIE " ) + appVer.indexOf( "Internet Explorer " );
-var isnav = ( navigator.appName.indexOf( "Netscape" ) >= 0 );
-var msieWin31 = (appVer.indexOf( "Windows 3.1" ) > 0);
-var ver = 0;
-var g_done = 0;
-var g_prevotlobjidx = 0;
-var g_ShowFSDefault = 0;
-var g_lastVisibleSld = 1;
-var g_allHidden = false;
-function IsIE() {
- return (msie >= 0 );
-}
-
-function IsNav() {
- return (isnav);
-}
-var msiePos = appVer.indexOf( "MSIE " );
-var inexPos = appVer.indexOf( "Internet Explorer " );
-if ( msiePos >= 0 )
- ver = parseFloat( appVer.substring( msiePos+5, appVer.indexOf ( ";", msiePos ) ) );
-else if( inexPos >= 0 )
- ver=parseFloat( appVer.substring( inexPos+18, appVer.indexOf(";",inexPos) ) )
-else
- ver = parseInt( appVer );
-
-//var g_supportsPPTHTML = 0; //!msieWin31 && ( ( msie >= 0 && ver >= 3.02 ) || ( msie < 0 && ver >= 3 ) );
-
-function GetCurrentSlideNum()
-{
- obj = GetHrefObj( g_currentSlide );
- if ( GetHrefObj( g_currentSlide ).m_origVisibility == 1 )
- return obj.m_slideIdx;
- else
- return g_currentSlide;
-}
-
-function GetNumSlides()
-{
- if ( GetHrefObj( g_currentSlide ).m_origVisibility == 1 )
- return NUM_VISIBLE_SLIDES;
- else
- return g_docTable.length;
-}
-
-function GetHrefObj( slideIdx )
-{ return g_docTable[slideIdx - 1];
-}
-
-function GetSlideNum( slideHref )
-{
- for (ii=0; ii<g_docTable.length; ii++) {
- if ( g_docTable[ii].m_slideHref == slideHref )
- return ii+1;
- }
- return 1;
-}
-
-function GoToNextSld()
-{
- targetIdx = g_currentSlide + 1;
- if ( GetHrefObj( targetIdx-1 ).m_origVisibility == 0 ) {
- if ( targetIdx<=g_docTable.length ) {
- obj = GetHrefObj( targetIdx );
- obj.m_visibility = 1;
- GoToSld( obj.m_slideHref );
- }
- }
- else {
- obj = GetHrefObj( targetIdx );
- while ( obj && ( obj.m_origVisibility == 0 ) && ( targetIdx<=g_docTable.length ) )
- obj = GetHrefObj( targetIdx++ );
- if( obj && obj.m_origVisibility )
- GoToSld( obj.m_slideHref );
- }
-}
-
-function GoToPrevSld()
-{
- targetIdx = g_currentSlide - 1;
- if ( targetIdx > 0 ) {
- obj = GetHrefObj( targetIdx );
- while ( ( obj.m_visibility == 0 ) && ( targetIdx>0 ) )
- obj = GetHrefObj( targetIdx-- );
- GoToSld( obj.m_slideHref );
- }
-}
-
-function GoToLast()
-{
- targetIdx = g_docTable.length;
- if ( targetIdx != g_currentSlide )
- GoToSld( GetHrefObj( targetIdx ).m_slideHref );
-}
-
-function GoToFirst()
-{ GoToSld( GetHrefObj(1).m_slideHref );
-}
-
-function highlite() {
- if ( IsFullScrMode() )
- return;
- index = GetCurrentSlideNum();
- if ( !frames[MAIN_FRAME].frames[OUTLINE_FRAME] )
- return;
- if ( msie < 0 ) {
- if ( g_prevotlobjidx != 0 ) {
- eval( "otlobj = frames[MAIN_FRAME].frames[OUTLINE_FRAME].document.LAYERID" + g_prevotlobjidx );
- otlobj.hidden = true;
- }
- else
- index = GetCurrentSlideNum();
- eval( "otlobj = frames[MAIN_FRAME].frames[OUTLINE_FRAME].document.LAYERID" + index );
- otlobj.hidden = false;
-
- g_prevotlobjidx = index;
-
- return;
- }
- if ( !g_showoutline )
- return;
-
- backclr = frames[MAIN_FRAME].frames[OUTLINE_FRAME].document.body.bgColor;
- textclr = frames[MAIN_FRAME].frames[OUTLINE_FRAME].document.body.text;
- if ( g_prevotlobjidx != 0 ) {
- eval( "otlobj = frames[MAIN_FRAME].frames[OUTLINE_FRAME].document.all.p" + g_prevotlobjidx );
- otlobj.style.backgroundColor = backclr;
- otlobj.style.color = textclr;
- otlobj.all.AREF.style.color = textclr;
- }
- else
- index = GetCurrentSlideNum();
- eval( "otlobj = frames[MAIN_FRAME].frames[OUTLINE_FRAME].document.all.p" + index );
- otlobj.style.backgroundColor = textclr;
- otlobj.style.color = backclr;
- otlobj.all.AREF.style.color = backclr;
- g_prevotlobjidx = index;
-}
-
-function ChangeFrame( frame, href )
-{
-if ( IsFramesMode() ) {
- if ( NAVBAR_FRAME == frame || OUTLINE_NAVBAR_FRAME == frame ) {
- frames[frame].location.replace(href);
- }
- else if( ! ( ( OUTLINE_FRAME == frame && !g_showoutline) || (NOTES_FRAME == frame && !g_shownotes ) ) ){
- frames[MAIN_FRAME].frames[frame].location.href = href;
- }
- }
- else {
- if ( frame == NAVBAR_FRAME || frame == SLIDE_FRAME ) {
- if( frame == NAVBAR_FRAME ) {
- href = FS_NAVBAR_HREF;
-
- }
- if( frame == NAVBAR_FRAME )
- window.frames[frame].location.replace(href);
- else
- window.frames[frame].location.href = href;
- }
- }
-
-}
-
-function shutEventPropagation() {
- if ( IsNav() )
- return;
-
- var slideFrame;
- if ( IsFramesMode() )
- slideFrame = frames[MAIN_FRAME].frames[SLIDE_FRAME];
- else
- slideFrame = window.frames[SLIDE_FRAME];
- if ( slideFrame.event )
- slideFrame.event.cancelBubble=true;
-}
-
-function GoToSld( slideHref )
-{
- shutEventPropagation();
- if ( slideHref != GetHrefObj( g_currentSlide ).m_slideHref || g_slideType != GetHrefObj( g_currentSlide ).type) {
- g_prevSlide = g_currentSlide;
- g_currentSlide = GetSlideNum( slideHref );
- g_slideType = GetHrefObj( g_currentSlide ).type;
- obj = GetHrefObj( g_currentSlide );
- obj.m_visibility = 1;
- ChangeFrame( SLIDE_FRAME, slideHref );
- if( !SIMPLE_FRAMESET )
- ChangeFrame( NOTES_FRAME, obj.m_notesHref );
- ChangeFrame( NAVBAR_FRAME, NAVBAR_HREF );
-
- }
-}
-
-function PrevSldViewed()
-{ GoToSld( GetHrefObj( g_prevSlide ).m_slideHref );
-}
-
-function NoHref() {}
-
-function ExpandOutline( )
-{
- g_outline_href = OUTLINE_EXPAND_HREF;
- ChangeFrame( OUTLINE_FRAME, OUTLINE_EXPAND_HREF );
- frames[OUTLINE_NAVBAR_FRAME].location.replace( OUTLINE_NAVBAR_HREF);
-}
-
-function CollapseOutline()
-{
- g_outline_href = OUTLINE_COLLAPSE_HREF;
- ChangeFrame( OUTLINE_FRAME, OUTLINE_COLLAPSE_HREF );
- frames[OUTLINE_NAVBAR_FRAME].location.replace( OUTLINE_NAVBAR_HREF);
- }
-
-function SlideUpdated( id )
-{
- if ( id != GetHrefObj( g_currentSlide ).m_slideHref ) {
- g_prevSlide = g_currentSlide;
- g_currentSlide = GetSlideNum( id );
- obj = GetHrefObj( g_currentSlide );
- if( !SIMPLE_FRAMESET )
- ChangeFrame( NOTES_FRAME, obj.m_notesHref );
- ChangeFrame( NAVBAR_FRAME, NAVBAR_HREF );
- }
-}
-
-function hrefList( slideHref, notesHref, visible, slideIdx, type )
-{
- this.m_slideHref = slideHref;
- this.m_notesHref = notesHref;
- this.m_navbarHref = NAVBAR_HREF;
- this.m_origVisibility = visible;
- this.m_visibility = visible;
- this.m_slideIdx = slideIdx;
- this.type = type;
-}
-
-function IsFullScrMode() {
- return g_fullscrMode;
-}
-
-
-function IsFramesMode() {
- return (1 - g_fullscrMode);
-}
-
-function SldUpdated( id )
-{
- if ( ( id != GetHrefObj( g_currentSlide ).m_slideHref ) || ( g_currentSlide == g_lastVisibleSld ) ){
- g_prevSlide = g_currentSlide;
- g_currentSlide = GetSlideNum( id );
- obj = GetHrefObj( g_currentSlide );
- if( !SIMPLE_FRAMESET )
- ChangeFrame( NOTES_FRAME, obj.m_notesHref );
- ChangeFrame( NAVBAR_FRAME, NAVBAR_HREF );
- }
-}
-
-function ToggleOutline() {
- g_showoutline = 1 - g_showoutline;
- writeMyFrame();
-}
-
-function ShowHideNotes() {
- g_shownotes = 1 - g_shownotes;
- writeMyFrame();
-}
-
-function writeMyFrame() {
- SetFSMode(0);
- obj = frames[MAIN_FRAME];
-
- var curslide = g_baseURL + "/" + GetHrefObj( g_currentSlide ).m_slideHref;
- var curnotes = g_baseURL + "/" + GetHrefObj( g_currentSlide ).m_notesHref;
- var otlhref = g_baseURL + "/" + g_outline_href;
- if ( msie < 0 ) {
- if ( ! g_showoutline && g_shownotes ) {
- obj.document.write( '<HTML><HEAD><SCRIPT language=JavaScript src=' + g_baseURL + '/script.js></SCRIPT><SCRIPT> base = parent; <\/SCRIPT><\/HEAD> \
- <frameset rows=\"*,20%\" id=\"frameset2\" > \
- <frame src=\"' + curslide + '\" name=PPTSld marginheight=0 marginwidth=0> \
- <frame src=\"' + curnotes + '\" name=PPTNts marginheight=0 marginwidth=0> \
- </frameset> </html>' );
- }
- else if( g_showoutline && g_shownotes ){
- obj.document.write( '<HTML><HEAD><SCRIPT language=JavaScript src=' + g_baseURL + '/script.js></SCRIPT><SCRIPT> base = parent; <\/SCRIPT><\/HEAD> \
- <frameset cols=\"20%,*\" id=\"frameset1\"> \
- <frame src=\"' + otlhref + '\" name=PPTOtl> \
- <frameset rows=\"*,20%\" id=\"frameset2\" > \
- <frame src=\"' + curslide + '\" name=PPTSld marginheight=0 marginwidth=0> \
- <frame src=\"' + curnotes + '\" name=PPTNts marginheight=0 marginwidth=0> \
- </frameset> </frameset></html>' );
- }
- else if ( !g_shownotes && !g_showoutline ) {
- obj.document.write( '<HTML><HEAD><SCRIPT language=JavaScript src=' + g_baseURL + '/script.js></SCRIPT><SCRIPT> base = parent; <\/SCRIPT><\/HEAD> \
- <frameset rows="*,0" frameborder=0 > \
- <frame src=\"' + curslide + '\" name=PPTSld marginheight=0 marginwidth=0> \
- </frameset> </html>' );
- }
- else if( !g_shownotes && g_showoutline ) {
- obj.document.write( '<HTML><HEAD><SCRIPT language=JavaScript src=' + g_baseURL + '/script.js></SCRIPT><SCRIPT> base = parent; <\/SCRIPT><\/HEAD> \
- <frameset cols=\"20%,*\" id=\"frameset1\"> \
- <frame src=\"' + otlhref + '\" name=PPTOtl> \
- <frame src=\"' + curslide + '\" name=PPTSld marginheight=0 marginwidth=0> \
- </frameset></html>' );
- }
- obj.document.close();
- }
- else {
- if ( g_showoutline ) {
- obj.PPTHorizAdjust.cols = "20%,*";
- obj.PPTOtl.location.reload();
- }
- else {
- obj.PPTHorizAdjust.cols = "0,*";
- }
- if ( g_shownotes ) {
- obj.PPTVertAdjust.rows = "*,20%";
- obj.PPTNts.location.href = curnotes;
- }
- else {
- obj.PPTVertAdjust.rows = "*,0";
- }
- }
- ChangeFrame( OUTLINE_NAVBAR_FRAME, OUTLINE_NAVBAR_HREF );
-}
-
-function FullScreen() {
- g_done = 0;
-
-
- SetFSMode(1);
- if ( msie >= 0 )
- FSWin = window.open( g_baseURL + "/" + "fullscreen.htm", null, "fullscreen=yes");
- else {
- var height = screen.availHeight;
- if ( window.navigator.platform.indexOf( "Mac" ) >= 0 ) {
- height -= 30;
- }
- FSWin = window.open( g_baseURL + "/" + "fullscreen.htm", "null", "height="+ height + ",width=" + screen.availWidth + ",screenX=0,screenY=0");
- }
-}
-
-function SetFSMode( i ) {
-
-}
-
-function Slide( i ) {
- SetFSMode(0);
- GoToSld(GetHrefObj(i).m_slideHref);
-}
-
-function TP_GoToNextSld() {
- SetFSMode(0);
- GoToNextSld();
-}
-
-function TP_GoToPrevSld() {
- SetFSMode(0);
- GoToPrevSld();
-}
-
-function CloseFullScreen() {
- g_done = 0;
-
- if ( IsNav() ){
- if ( self.opener )
- opener.FSWin = null;
- }
- window.close();
-}
-
-function slidenum(i) {
- var slidename = "slide";
- if ( i < 10 )
- return ( slidename + "000" + i);
- else if ( i < 100 )
- return ( slidename + "00" + i );
- else if ( i < 1000 )
- return (slidename + "0" + i );
- else
- return (slidename + i );
-}
-function UpdateLastVisibleSlide( index ) {
- if ( g_lastVisibleSld < index )
- g_lastVisibleSld = index;
-}
-
-function jpegArray( numSlides ) {
-count_hidden = 0;
- g_docTable = new Array();
- for( i=0; i<numSlides; i++ ) {
- j = 2 * numSlides + i + 1;
- var str = slidenum( j ) +".htm";
- if( g_notesTable[i] == 1 )
- g_docTable[i] = new hrefList( str, slidenum(i+1 ) + "_notes_pane.htm", g_hiddenSlide[i], i+1-count_hidden, "jpeg" );
- else
- g_docTable[i] = new hrefList( str, BLANK_NOTES_HREF, g_hiddenSlide[i], i+1-count_hidden, "jpeg" );
- if ( !g_hiddenSlide[i] ) count_hidden++;
- else UpdateLastVisibleSlide( i+1 );
- }
-}
-
-function ieArray( numSlides ) {
-count_hidden = 0;
- g_docTable = new Array();
- for( i=0; i<numSlides; i++ ) {
- var str = slidenum(i+1) +".htm";
- if( g_notesTable[i] == 1 )
- g_docTable[i] = new hrefList( str, slidenum( i+1 ) + "_notes_pane.htm", g_hiddenSlide[i], i+1-count_hidden, "ie" );
- else
- g_docTable[i] = new hrefList( str, BLANK_NOTES_HREF, g_hiddenSlide[i], i+1-count_hidden, "ie" );
- if ( !g_hiddenSlide[i] ) count_hidden++;
- else UpdateLastVisibleSlide( i+1 );
- }
-}
-
-function navArray( numSlides ) {
-count_hidden = 0;
- g_docTable = new Array();
- for( i=0; i<numSlides; i++ ) {
- j = numSlides + i + 1;
- var str = slidenum( j ) +".htm";
- if( g_notesTable[i] == 1 )
- g_docTable[i] = new hrefList( str, slidenum(i+1 ) + "_notes_pane.htm", g_hiddenSlide[i], i+1-count_hidden, "nav" );
- else
- g_docTable[i] = new hrefList( str, BLANK_NOTES_HREF, g_hiddenSlide[i], i+1-count_hidden, "nav" );
- if ( !g_hiddenSlide[i] ) count_hidden++;
- else UpdateLastVisibleSlide( i+1 );
- }
-}
-
-function LoadHTMLVersion() {
- var os = window.navigator.platform.indexOf("Mac");
- if ( (msie || isnav ) && ( (os < 0 && ver >= 4 ) || ( os >= 0 && ver >= 5 ) || (os >=0 && msie < 0 && ver >= 4 ) ) ){
- if ( msie >= 0 ) {
- if ( isIEFiles > 0 )
- ieArray( 32 );
- else if ( isFLATFiles > 0 ){
- /*if ( IsFramesMode() )
- StatusPlay("This presentation is optimized for use with older versions of your browser. Since you are using a more recent version of Microsoft Internet Explorer or Netscape Navigator, consider optimizing this presentation to take advantage of your current version's advanced capabilities."); */
- jpegArray( 32 );
- }
- else
- window.location.replace( "WebQTLDemo_files/error.htm" );
- }
- else {
- if ( isNAVFiles > 0 && ver < 5)
- navArray( 32 );
- else if ( isFLATFiles > 0 ) {
- /* if ( IsFramesMode() )
- StatusPlay("This presentation is optimized for use with older versions of your browser. Since you are using a more recent version of Microsoft Internet Explorer or Netscape Navigator, consider optimizing this presentation to take advantage of your current version's advanced capabilities."); */
- jpegArray( 32 );
- }
- else
- window.location.replace( "WebQTLDemo_files/error.htm" );
- }
-}
-else {
- /*
- if ( IsFramesMode() && !isWebTV() )
- StatusPlay("This presentation contains content that your browser may not be able to show properly. This presentation was optimized for more recent versions of Microsoft Internet Explorer or Netscape Navigator.");
- */
- if ( isFLATFiles <= 0 ) {
- /* if ( IsFramesMode() )
- window.alert("This presentation contains content that your browser may not be able to display properly. This presentation is optimized for more recent versions of Microsoft Internet Explorer or Netscape Navigator." );
- */
- window.location.replace( "WebQTLDemo_files/error.htm" );
- }
- /*
- else if ( IsFramesMode() && !isWebTV() )
- StatusPlay( "This presentation contains content that your browser may not be able to show properly. This presentation was optimized for more recent versions of Microsoft Internet Explorer or Netscape Navigator." );
- */
- jpegArray ( 32 );
- }
-
-}
-
-function isWebTV() {
- if ( window.navigator.appName.indexOf( 'WebTV' ) >= 0 )
- return true;
- return false;
-}
-
-var count;
-var statusText;
-var statusfirst = 0;
- function display50( text )
-{
- len = text.length;
- if ( len < 50 && count < 2) {
- window.status = text;
- window.setTimeout( "repeat()", 300 );
- }
- else {
- var period = 200;
- window.status = text;
- newtext = text.substring( 1, len );
- if ( statusfirst ) {
- statusfirst = 0;
- period = 2000;
- }
- window.setTimeout( "display50( newtext )", period );
- }
-}
-function repeat( ) {
- count++;
- statusfirst = 1;
- display50( statusText );
-}
-
-function StatusPlay( text ) {
- count = 0;
- statusText = text;
- repeat( );
- }
-function makeSlide( i, notes, visible ) {
- g_notesTable[i] = notes;
- g_hiddenSlide[i] = visible;
-}
-
diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0001.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0001.htm
deleted file mode 100755
index 4c783452..00000000
--- a/web/tutorial/ppt/WebQTLDemo_files/slide0001.htm
+++ /dev/null
@@ -1,25 +0,0 @@
-<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> <meta name=Description content="Jun-19-03: WebQTL Demonstration One please link to www.webqtl.org/search.html"> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
-gId="slide0001.htm"
-if ( !parent.base.g_done && (parent.base.msie < 0 ) ) {
- parent.base.g_done = 1;
- document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref);
-}
-if( !IsNts() ) Redirect( "PPTSld", gId );
-var g_hasTrans = true, g_autoTrans = false, g_transSecs = 1;
-//-->
-</script><script for=window event=onload language=JavaScript><!--
-if( !IsSldOrNts() ) return;
-if( MakeNotesVis() ) return;
-LoadSld( gId );
-playList();MakeSldVis(1);
-//-->
-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:6.89%;left:3.31%;width:96.42%;height:19.61%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";mso-margin-left-alt:232; mso-text-indent-alt:0;position:absolute;top:0%;left:1.09%;width:97.39%; height:100.0%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:68%'><i>WebQTL Demonstration One<br> </i></span></span><span style='position:absolute;top:33.33%;left:4.23%;width:95.76%'><span style='font-size:68%'><i>please link to <br> </i></span></span><span style='position:absolute;top:58.55%;left:4.23%;width:95.76%'><span style='font-size:86%'><i>www.webqtl.org/search.html</i></span><span style='font-size:68%;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <div style='position:absolute;top:31.62%;left:5.43%;width:73.24%;height:45.05%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='position:absolute;top:0%;left:2.71%;width:97.28%; height:93.33%'> <div style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:0%;width:89.77%;height:39.91%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='mso-special-format: nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts";font-size: 99%'>l</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>Part 1: How to discover shared </span></span><span style='position:absolute; top:30.52%;left:7.86%;width:86.54%'><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>expression patterns (slides </span></span><span style='position:absolute;top:61.05%;left:7.86%;width:86.54%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>2Ð14)<br> </span></span></div> <div style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:48.31%;left:0%;width:84.94%;height:27.73%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='mso-special-format: nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts";font-size: 99%'>l</span><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>Part 2. Discovering upstream </span></span><span style='position:absolute; top:43.93%;left:8.31%;width:91.46%'><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>modulators (15Ð25)<br> </span></span></div> <div style='mso-line-spacing:" 0 &#1;";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:84.45%;left:0%;width:100.0%;height:15.54%'><span style='position:absolute;top:0%;left:7.06%;width:92.93%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'><span style='mso-special-format: "numbullet3\,3";color:#FBFDB8;position:absolute;left:-7.6%;font-family:Times; font-size:99%'>3.</span></span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>Discovering downstream targets</span></span></div> </div> </div> <img border=0 src="slide0001_image001.gif" style='position:absolute;top:21.9%; left:74.56%;width:23.57%;height:64.31%'> <div class=O style='position:absolute;top:79.32%;left:75.89%;width:10.46%; height:14.48%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:42.68%'><span style='font-family:"Arial Rounded MT Bold";font-size:233%;color:#6E6E6E'>RNA</span><span style='font-family:"Arial Rounded MT Bold";font-size:300%;color:#6E6E6E; display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:46.34%;left:0%;width:82.27%;height:54.87%'><span style='font-family:"Arial Rounded MT Bold";font-size:300%;color:#6E6E6E; mso-special-format:lastCR;display:none'><br> </span></div> </div> <div class=O style='position:absolute;top:76.5%;left:4.37%;width:78.67%; height:13.07%'><span style='position:absolute;top:0%;left:0%;width:98.98%'><span style='font-family:"Gill Sans";font-size:250%;color:#E9EB5D;mso-color-index: 3'><i>PowerPoint ÒNormal viewÓ has notes that </i></span></span><span style='position:absolute;top:51.35%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:250%;color:#E9EB5D;mso-color-index: 3'><i>may be useful companions to these slides.</i></span><span style='font-family:"Gill Sans";font-size:250%;color:#E9EB5D;mso-color-index: 3;mso-special-format:lastCR;display:none'><br> </span></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Welcome to a short demonstation of WebQTL. Please adjust the wize of windows on your monitor so that you can see part of this page as well as WebQTL windows. WebQTL will produce a potentially large number of new windows (pop-ups), so you may need to modify your browser preferences to permit pop-ups.<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>In this demonstration, we explore one important transcript expressed in the brain: the amyloid beta precursor protein messenger RNA. The product of this mRNA, the APP protein, is associated with Alzheimer disease.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>(Initial version of June 2003 by Rob Williams, Last edits June 16, 2003 by RW.)</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Welcome to a short demonstation of WebQTL. Please adjust the wize of windows on your monitor so that you can see part of this page as well as WebQTL windows. WebQTL will produce a potentially large number of new windows (pop-ups), so you may need to modify your browser preferences to permit pop-ups.<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>In this demonstration, we explore one important transcript expressed in the brain: the amyloid beta precursor protein messenger RNA. The product of this mRNA, the APP protein, is associated with Alzheimer disease.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>(Initial version of June 2003 by Rob Williams, Last edits June 16, 2003 by RW.)</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:6.89%;left:2.78%;width:93.77%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:2.11%;width:96.46%;height:50.6%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>or webqtl.org/search.html (mirror)</span></div> </div> <img border=0 src="slide0002_image002.gif" style='position:absolute;top:16.6%; left:2.51%;width:71.65%;height:81.27%'><img border=0 src="slide0002_image003.gif" style='position:absolute;top:49.82%;left:42.38%; width:20.39%;height:7.59%'><img border=0 src="slide0002_image004.gif" style='position:absolute;top:50.35%;left:62.78%;width:15.09%;height:36.74%'> <div class=O style='position:absolute;top:60.42%;left:79.33%;width:15.76%; height:11.48%'> <div style='position:absolute;top:0%;left:0%;width:100.0%;height:49.23%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>choose a<br> </span></div> <div style='position:absolute;top:50.76%;left:0%;width:96.63%;height:49.23%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>database</span></div> </div> <div class=O style='position:absolute;top:46.46%;left:79.33%;width:21.05%; height:11.48%'> <div style='position:absolute;top:0%;left:0%;width:89.93%;height:49.23%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>enter<br> </span></div> <div style='position:absolute;top:50.76%;left:0%;width:100.0%;height:49.23%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'><i>amyloid beta</i></span><span style='font-family:"Gill Sans";font-size: 250%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR;display:none'><i><br> </i></span></div> </div> <div class=O style='position:absolute;top:78.26%;left:79.73%;width:11.52%; height:11.48%'> <div style='position:absolute;top:0%;left:0%;width:89.65%;height:49.23%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>select<br> </span></div> <div style='position:absolute;top:50.76%;left:0%;width:100.0%;height:49.23%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>search</span></div> </div> <div style='position:absolute;top:.88%;left:3.31%;width:93.77%;height:9.36%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div style='position:absolute;top:0%;left:1.12%;width:97.45%;height:79.24%'> <div class=B1 style='mso-margin-left-alt:644;mso-text-indent-alt:420'></div> <div class=B2 style='mso-margin-left-alt:937;mso-text-indent-alt:716'></div> <div class=B3 style='mso-margin-left-alt:1232;mso-text-indent-alt:1009'></div> <div class=B4 style='mso-margin-left-alt:1544;mso-text-indent-alt:1304'></div> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 0;position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>link to www.webqtl.org/search.html</span></div> </div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>WebQTL can be used to enter your own trait data or to work with data that we have entered for you.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Linking to http://www.webqtl.org/search.html will get you a version of the window above. It may not be identical in layout but it will have the key features. Please follow the intructions on the slide. Of course, we encourage you to enter your own terms of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Two points: If you make a search term too complex you may get no hits. if you make it too simple (for example, APP) then you may get too much. Experiment.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>If you just link to</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>http://www.webqtl.org you will NOT see the window above but will see text that will help you to enter your own data.<span style="mso-spacerun: yes">&nbsp; </span>To get to a version of the window shown above you will need to click on the phrase<span style="mso-spacerun: yes">&nbsp; </span>RNA expression and Phenotype Databases in the upper banner.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>If you do not get a new page within 30 seconds then there is<span style="mso-spacerun: yes">&nbsp; </span>a problem: try the mirror site http://webqtl.org/search.html.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>WebQTL can be used to enter your own trait data or to work with data that we have entered for you.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Linking to http://www.webqtl.org/search.html will get you a version of the window above. It may not be identical in layout but it will have the key features. Please follow the intructions on the slide. Of course, we encourage you to enter your own terms of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Two points: If you make a search term too complex you may get no hits. if you make it too simple (for example, APP) then you may get too much. Experiment.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>If you just link to</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>http://www.webqtl.org you will NOT see the window above but will see text that will help you to enter your own data.<span style="mso-spacerun: yes">&nbsp; </span>To get to a version of the window shown above you will need to click on the phrase<span style="mso-spacerun: yes">&nbsp; </span>RNA expression and Phenotype Databases in the upper banner.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>If you do not get a new page within 30 seconds then there is<span style="mso-spacerun: yes">&nbsp; </span>a problem: try the mirror site http://webqtl.org/search.html.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'><img border=0 src="slide0003_image005.gif" style='position:absolute;top:9.36%; left:9.13%;width:59.73%;height:87.45%'><img border=0 src="slide0003_image006.gif" style='position:absolute;top:72.26%;left:14.03%; width:62.51%;height:6.53%'> <div class=O style='position:absolute;top:68.37%;left:78.54%;width:21.05%; height:17.31%'> <div style='position:absolute;top:0%;left:0%;width:89.93%;height:32.65%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>highlight<br> </span></div> <div style='position:absolute;top:33.67%;left:0%;width:100.0%;height:32.65%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'><i>amyloid beta<br> </i></span></div> <div style='position:absolute;top:67.34%;left:0%;width:89.93%;height:32.65%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>then click</span><span style='font-family:"Gill Sans";font-size:250%; color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:-4.59%;left:7.81%;width:84.9%;height:18.55%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='position:absolute;top:24.76%;left:1.56%;width:96.87%; height:46.66%'><span style='font-size:91%'>Search results</span><span style='mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>If all goes well, you will see a version of this window. WebQTL will display up to about 100 hits. If a search generates larger numbers of hits then you will need to refine your search terms.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
-function playList() {
-
-}
-//-->
-</script> </body> </html> \ No newline at end of file
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0003_notes_pane.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0003_notes_pane.htm
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--- a/web/tutorial/ppt/WebQTLDemo_files/slide0003_notes_pane.htm
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@@ -1,5 +0,0 @@
-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>If all goes well, you will see a version of this window. WebQTL will display up to about 100 hits. If a search generates larger numbers of hits then you will need to refine your search terms.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0004.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0004.htm
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-<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> <meta name=Description content=Jun-19-03> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
-gId="slide0004.htm"
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- parent.base.g_done = 1;
- document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref);
-}
-if( !IsNts() ) Redirect( "PPTSld", gId );
-var g_hasTrans = true, g_autoTrans = false, g_transSecs = 1;
-//-->
-</script><script for=window event=onload language=JavaScript><!--
-if( !IsSldOrNts() ) return;
-if( MakeNotesVis() ) return;
-LoadSld( gId );
-playList();MakeSldVis(1);
-//-->
-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.41%;left:1.72%;width:97.61%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:2.03%;width:97.96%;height:50.6%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>First page of data: The ÒTrait Data FormÓ</span></div> </div> <img border=0 src="slide0004_image007.gif" style='position:absolute;top:11.13%; left:3.31%;width:77.61%;height:86.39%'><img border=0 src="slide0004_image008.gif" style='position:absolute;top:56.0%;left:66.88%; width:14.7%;height:7.42%'> <div class=O style='position:absolute;top:50.7%;left:81.85%;width:20.0%; height:27.2%'> <div style='position:absolute;top:0%;left:0%;width:100.0%;height:20.12%'><span style='font-size:200%;color:#E9EB5D'><i>Click here<br> </i></span></div> <div style='position:absolute;top:19.48%;left:0%;width:83.44%;height:20.12%'><span style='font-size:200%;color:#E9EB5D'><i>to learn<br> </i></span></div> <div style='position:absolute;top:39.61%;left:0%;width:83.44%;height:20.12%'><span style='font-size:200%;color:#E9EB5D'><i>about<br> </i></span></div> <div style='position:absolute;top:59.09%;left:0%;width:83.44%;height:20.12%'><span style='font-size:200%;color:#E9EB5D'><i>data<br> </i></span></div> <div style='position:absolute;top:79.22%;left:0%;width:83.44%;height:20.12%'><span style='font-size:200%;color:#E9EB5D'><i>source</i></span></div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>The Trait Data and Editing Form is the single more important page from the point of view of working with WebQTL data. Please read the text carefully. Explore the links, but do not close this page. We will need it many more times in this demonstration.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
-function playList() {
-
-}
-//-->
-</script> </body> </html> \ No newline at end of file
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>The Trait Data and Editing Form is the single more important page from the point of view of working with WebQTL data. Please read the text carefully. Explore the links, but do not close this page. We will need it many more times in this demonstration.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0005.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0005.htm
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-<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> <meta name=Description content=Jun-19-03> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
-gId="slide0005.htm"
-if ( !parent.base.g_done && (parent.base.msie < 0 ) ) {
- parent.base.g_done = 1;
- document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref);
-}
-if( !IsNts() ) Redirect( "PPTSld", gId );
-var g_hasTrans = true, g_autoTrans = false, g_transSecs = 1;
-//-->
-</script><script for=window event=onload language=JavaScript><!--
-if( !IsSldOrNts() ) return;
-if( MakeNotesVis() ) return;
-LoadSld( gId );
-playList();MakeSldVis(1);
-//-->
-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.41%;left:3.31%;width:96.29%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:2.06%;width:97.93%;height:50.6%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>Data sources: Phenotpyes and genotypes</span></div> </div> <img border=0 src="slide0005_image009.gif" style='position:absolute;top:10.77%; left:7.68%;width:84.5%;height:88.33%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>There are already five databases in WebQTL. Each will eventually have a page like this describing the data source and appropriate citations to these databases. The great majority of data in WebQTL were generated in our own labs and those of our collaborators.<span style="mso-spacerun: yes">&nbsp; </span>We welcome you to use these data, but caution you that there are inevitably lots of little problems and issues that may compromise some results. Be cautious and skeptical. Ask us questions before you leap to publication. And please, if you find the data useful or can verify or refute data, LET US KNOW. We would like to add you to our reference section and add links to improvements.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
-function playList() {
-
-}
-//-->
-</script> </body> </html> \ No newline at end of file
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@@ -1,5 +0,0 @@
-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>There are already five databases in WebQTL. Each will eventually have a page like this describing the data source and appropriate citations to these databases. The great majority of data in WebQTL were generated in our own labs and those of our collaborators.<span style="mso-spacerun: yes">&nbsp; </span>We welcome you to use these data, but caution you that there are inevitably lots of little problems and issues that may compromise some results. Be cautious and skeptical. Ask us questions before you leap to publication. And please, if you find the data useful or can verify or refute data, LET US KNOW. We would like to add you to our reference section and add links to improvements.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> <meta name=Description content=Jun-19-03> <link rel=Stylesheet href="master03_stylesheet.css"> <style media=print> <!--.sld {left:0px !important; width:6.0in !important; height:4.5in !important; font-size:76% !important;} --> </style> <script language=JavaScript src=script.js></script><script language=JavaScript><!--
-gId="slide0006.htm"
-if ( !parent.base.g_done && (parent.base.msie < 0 ) ) {
- parent.base.g_done = 1;
- document.location.replace( parent.base.GetHrefObj(parent.base.g_currentSlide).m_slideHref);
-}
-if( !IsNts() ) Redirect( "PPTSld", gId );
-var g_hasTrans = true, g_autoTrans = false, g_transSecs = 1;
-//-->
-</script><script for=window event=onload language=JavaScript><!--
-if( !IsSldOrNts() ) return;
-if( MakeNotesVis() ) return;
-LoadSld( gId );
-playList();MakeSldVis(1);
-//-->
-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.06%;left:1.72%;width:47.94%;height:7.24%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:4.87%;left:4.14%;width:95.85%;height:78.04%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:81%;color:#E9EB5D;mso-color-index: 3'>Return to Trait Data page </span></div> </div> <img border=0 src="slide0006_image010.gif" style='position:absolute;top:7.77%; left:.79%;width:48.47%;height:79.32%'><img border=0 src="slide0006_image011.gif" style='position:absolute;top:7.77%;left:50.46%;width:48.6%;height:78.79%'> <div style='position:absolute;top:1.06%;left:52.45%;width:43.84%;height:13.78%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:2.56%;left:4.53%;width:92.44%;height:41.02%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:81%;color:#E9EB5D;mso-color-index: 3'>bottom of this page</span></div> </div> <div class=O style='position:absolute;top:86.39%;left:3.44%;width:109.13%; height:11.48%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>Trait data for each strain with SE when known. For array data </span></span><span style='position:absolute;top:50.76%;left:0%;width:99.02%'><span style='font-family:"Gill Sans";font-size:217%;color:#E9EB5D;mso-color-index: 3'>the scale is ~ log base 2.<span style="mso-spacerun: yes">&nbsp;&nbsp; </span></span><span style='font-size:183%;color:#E9EB5D;mso-color-index:3'>F1=13.752 = 2^13.752 = 13796 </span><span style='font-family:"Gill Sans";font-size:183%; color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR;display:none'><br> </span></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>This<span style="mso-spacerun: yes">&nbsp; </span>slide shows you the<span style="mso-spacerun: yes">&nbsp; </span>lower parts of the Trait Data Page. We expect to make many small modification of this page, so do not be surprised if some elements have been moved around.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
-function playList() {
-
-}
-//-->
-</script> </body> </html> \ No newline at end of file
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
- window.location.replace( "endshow.htm" );
-}
-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>This<span style="mso-spacerun: yes">&nbsp; </span>slide shows you the<span style="mso-spacerun: yes">&nbsp; </span>lower parts of the Trait Data Page. We expect to make many small modification of this page, so do not be surprised if some elements have been moved around.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Finally, we can now start an analysis.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>We ask a simple question:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Do differences in App transcript expression correlate with those of any other transcripts in the forebrain?</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.41%;left:3.31%;width:93.77%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:2.11%;width:96.46%;height:50.6%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>The </span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'><i>App</i></span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'> transcript neighborhood</span></div> </div> <img border=0 src="slide0008_image014.gif" style='position:absolute;top:10.77%; left:5.82%;width:66.62%;height:88.33%'> <div class=O style='position:absolute;top:30.74%;left:75.23%;width:26.22%; height:18.72%'><span style='position:absolute;top:0%;left:0%;width:91.91%'><span style='font-size:150%;color:#E9EB5D;mso-color-index:3'><b>Question: </b></span><span style='font-size:133%;color:#E9EB5D;mso-color-index:3'><b>How </b></span></span><span style='position:absolute;top:21.69%;left:0%;width:97.47%'><span style='font-size:133%;color:#E9EB5D;mso-color-index:3'><b>many transcripts </b></span></span><span style='position:absolute;top:40.56%;left:0%;width:100.0%'><span style='font-size:133%;color:#E9EB5D;mso-color-index:3'><b>have correlations </b></span></span><span style='position:absolute;top:59.43%;left:0%;width:97.97%'><span style='font-size:133%;color:#E9EB5D;mso-color-index:3'><b>&gt;0.7? What does </b></span></span><span style='position:absolute;top:79.24%;left:0%;width:85.35%'><span style='font-size:133%;color:#E9EB5D;mso-color-index:3'><b>this imply.</b></span></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>The answer is a strong Yes. A very large number of transcripts have correlations above 0.7 (absolute value) with App mRNA. The precise number today is 208. But this will change as we add more strains and arrays. In any case, this is a fairly large number and all of these correlations are significant at alpha .05 even when correcting for the enormous numbers<span style="mso-spacerun: yes">&nbsp; </span>of tests (12422 tests).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>What does this imply?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>That there can be massive codependence of expression variance among transcripts. App is NOT an isolated instance. This is improtant biologically and statistically. From a statistical perspective, we would like to know how many ÒindependentÓ test we effectively are performing when we use array data in this way. Are we testing 12000 independent transcripts or just 1200 transcriptional ÒmodulesÓ each with blurred boarders but each with about 10 effective members. There is no answer yet, but we probaby have a large enough data set to begin to answer this question.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>The answer is a strong Yes. A very large number of transcripts have correlations above 0.7 (absolute value) with App mRNA. The precise number today is 208. But this will change as we add more strains and arrays. In any case, this is a fairly large number and all of these correlations are significant at alpha .05 even when correcting for the enormous numbers<span style="mso-spacerun: yes">&nbsp; </span>of tests (12422 tests).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>What does this imply?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>That there can be massive codependence of expression variance among transcripts. App is NOT an isolated instance. This is improtant biologically and statistically. From a statistical perspective, we would like to know how many ÒindependentÓ test we effectively are performing when we use array data in this way. Are we testing 12000 independent transcripts or just 1200 transcriptional ÒmodulesÓ each with blurred boarders but each with about 10 effective members. There is no answer yet, but we probaby have a large enough data set to begin to answer this question.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:2.47%;left:6.62%;width:94.17%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:2.1%;width:98.03%;height:50.6%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>Handdrawn sketch of the neighborhood</span></div> </div> <img border=0 src="slide0009_image015.gif" style='position:absolute;top:13.07%; left:9.53%;width:80.79%;height:80.38%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Many of the data types in the previous slide are hot-linked and it is easy to generate a small web of correlations between any transcript of interest and many other transcripts. In this case, we have used green lines between transcripts that have positive correlations, and red lines between transcripts that have negative correlations. Correlations have been multiplied by 100. The correlation of 0.96 between App and Hsp84-1 reads 96.<span style="mso-spacerun: yes">&nbsp; </span>These are Pearson product moment correlations and they are sensitive to outliers. If you prefer, you can recompute Spearman rank order correlations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Where did Ndr4 (lower left) come from? It is not in the list in the previous slide. Actually it is. Nomenclature changes rapidly. If you click on R74996 in the previous slide (the active webqtl version) you will see that it now has a new symbol and name.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>What are all of the<span style="mso-spacerun: yes">&nbsp; </span>conventions in this correlation network sketch.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Times size=3>1.</font><font face=Helvetica size=3>The official gene symbol = App</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. OUr estimate of the location of these gene in the Mouse Genome Sequencing Consoritum version 3 build (MGSCv3). Chromosome followed by the megabase position relative to the centromere. (Mice only have one chromosome arm so this is an unambiguous coordinate. )</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>3. The pair of numbers: top is the highest expression among the strain set. The lower number is the lowest expression of that transcript among the strain set.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>4. Vertical number on the right side of each box: this is the probe set ID given by Affymetrix. We have truncated these probe set IDs so you will not see the usual<span style="mso-spacerun: yes">&nbsp; </span>ÒatÓ. A single gene may be represented by more than 10 probe sets. Thus this ID number is essential to identify the actual data source.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>5. Lower right corner: a two digit number followed by plus and minus signs. These numbers are the correlation value (absolute value) of the 100th best correlated transcript. The plus and minus signs indicate the mean polarity of the correlations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>6. The set of numbers that read 2@140* etc. These are the approximate locations of additive effect QTLs detected by WebQTL that we will describe in other slides. Read this as: App has a suggestive QTL on Chr 2 at about 140 Mb and the D allele inherited from DBA/2J confirms a higher expression level at this marker.<span style="mso-spacerun: yes">&nbsp; </span>If there is no star symbol, then it is not even formally ÒsuggestiveÓ but does make an interesting looking blip on the QTL radar screen.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Many of the data types in the previous slide are hot-linked and it is easy to generate a small web of correlations between any transcript of interest and many other transcripts. In this case, we have used green lines between transcripts that have positive correlations, and red lines between transcripts that have negative correlations. Correlations have been multiplied by 100. The correlation of 0.96 between App and Hsp84-1 reads 96.<span style="mso-spacerun: yes">&nbsp; </span>These are Pearson product moment correlations and they are sensitive to outliers. If you prefer, you can recompute Spearman rank order correlations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Where did Ndr4 (lower left) come from? It is not in the list in the previous slide. Actually it is. Nomenclature changes rapidly. If you click on R74996 in the previous slide (the active webqtl version) you will see that it now has a new symbol and name.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>What are all of the<span style="mso-spacerun: yes">&nbsp; </span>conventions in this correlation network sketch.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Times size=2>1.</font><font face=Helvetica size=2>The official gene symbol = App</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>2. OUr estimate of the location of these gene in the Mouse Genome Sequencing Consoritum version 3 build (MGSCv3). Chromosome followed by the megabase position relative to the centromere. (Mice only have one chromosome arm so this is an unambiguous coordinate. )</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>3. The pair of numbers: top is the highest expression among the strain set. The lower number is the lowest expression of that transcript among the strain set.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>4. Vertical number on the right side of each box: this is the probe set ID given by Affymetrix. We have truncated these probe set IDs so you will not see the usual<span style="mso-spacerun: yes">&nbsp; </span>ÒatÓ. A single gene may be represented by more than 10 probe sets. Thus this ID number is essential to identify the actual data source.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>5. Lower right corner: a two digit number followed by plus and minus signs. These numbers are the correlation value (absolute value) of the 100th best correlated transcript. The plus and minus signs indicate the mean polarity of the correlations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>6. The set of numbers that read 2@140* etc. These are the approximate locations of additive effect QTLs detected by WebQTL that we will describe in other slides. Read this as: App has a suggestive QTL on Chr 2 at about 140 Mb and the D allele inherited from DBA/2J confirms a higher expression level at this marker.<span style="mso-spacerun: yes">&nbsp; </span>If there is no star symbol, then it is not even formally ÒsuggestiveÓ but does make an interesting looking blip on the QTL radar screen.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:3.53%;left:2.11%;width:97.48%;height:21.02%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";mso-margin-left-alt:232; mso-text-indent-alt:0;position:absolute;top:18.48%;left:.95%;width:99.04%; height:61.34%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:68%'><i>Are there experimental results to corroborate a link </i></span></span><span style='position:absolute;top:49.31%;left:4.25%;width:90.12%'><span style='font-size:68%'><i>between App with Hsp84-1?</i></span></span></div> </div> <div style='position:absolute;top:82.15%;left:4.1%;width:96.42%;height:18.55%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'></div> <div class=O style='position:absolute;top:29.5%;left:4.37%;width:99.33%; height:13.07%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:250%;color:#E9EB5D'><i>Yes: Heat shock 84-1 induces the expression of App, </i></span></span><span style='position: absolute;top:51.35%;left:0%;width:85.33%'><span style='font-family:"Gill Sans"; font-size:250%;color:#E9EB5D'><i>ubiquitin, and pyruvate kinase</i></span></span></div> <div class=O style='position:absolute;top:50.88%;left:5.16%;width:95.23%; height:19.78%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:250%;color:#E9EB5D'><i>Having ÒconfirmedÓ these known relations, we can </i></span></span><span style='position:absolute;top:33.92%;left:0%;width:99.72%'><span style='font-family:"Gill Sans";font-size:250%;color:#E9EB5D'><i>now add new members to this family: Atp6l, Gnas, </i></span></span><span style='position: absolute;top:67.85%;left:0%;width:89.01%'><span style='font-family:"Gill Sans"; font-size:250%;color:#E9EB5D'><i>Ndr4. A thin veneer of functional genomics.</i></span></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Having worked with WebQTL now for 30 minutes, do we know anything new? The hypothesis that we have generated (but not validated) is that three transcripts: Atp6l, Gnas, and Ndr4 are part of a family of genes that are coregulated in normal mouse forebrain with App and Hsp84-1. We need to add functional and mechanistic significance to this hypothesis to make it biologically vibrant. But from a statiistical standpoint it is a strong inference.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Please donÕt say: But these are mere correlations. A high correlation in this context has a biological basis. The real question is are we smart enough to understand the web (not chain) of causality that produced the correlation. Once we understand the web of causality, does it have utility? Very often the answer will be NO. This will often be the case when a high correlation is generated by linkage disequilibrium of sets of polymorphisms that modulate a set of mechanistically separated traits. Chromosomal linkage can produce correlations that are not mechanistic in the conventional sense used by molecular biologists. For example, clusters<span style="mso-spacerun: yes">&nbsp; </span>of hox transcription factor genes tend to be close physically to keratin gene clusters, and one might expect shared patterns of variance produced by this linkage in a mapping panel, no matter how large.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>If Affymetrix designed probe sets with reasonable care, if we did the experiments correctly, if we sampled animals appropriately, then a correlation of 0.70 or higher between transcripts in the brain tells you that these two transcripts are effectively coupled in this set of animals under this set of conditions. More than 50% the variance in the expression of one transcript can be predicted from the other. That is a major piece of information that could be of significant clinical, economic, and predictive value, whatever its causes. Yes, correlation coefficients are noisy and have large error terms, but we have larger n of strains coming to the rescue. Expect more than 50 BXD lines soon.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>This is a thin veneer of functional genomics. It is enough to generate some marvelous hypotheses in a semi-automated way.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Having worked with WebQTL now for 30 minutes, do we know anything new? The hypothesis that we have generated (but not validated) is that three transcripts: Atp6l, Gnas, and Ndr4 are part of a family of genes that are coregulated in normal mouse forebrain with App and Hsp84-1. We need to add functional and mechanistic significance to this hypothesis to make it biologically vibrant. But from a statiistical standpoint it is a strong inference.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Please donÕt say: But these are mere correlations. A high correlation in this context has a biological basis. The real question is are we smart enough to understand the web (not chain) of causality that produced the correlation. Once we understand the web of causality, does it have utility? Very often the answer will be NO. This will often be the case when a high correlation is generated by linkage disequilibrium of sets of polymorphisms that modulate a set of mechanistically separated traits. Chromosomal linkage can produce correlations that are not mechanistic in the conventional sense used by molecular biologists. For example, clusters<span style="mso-spacerun: yes">&nbsp; </span>of hox transcription factor genes tend to be close physically to keratin gene clusters, and one might expect shared patterns of variance produced by this linkage in a mapping panel, no matter how large.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>If Affymetrix designed probe sets with reasonable care, if we did the experiments correctly, if we sampled animals appropriately, then a correlation of 0.70 or higher between transcripts in the brain tells you that these two transcripts are effectively coupled in this set of animals under this set of conditions. More than 50% the variance in the expression of one transcript can be predicted from the other. That is a major piece of information that could be of significant clinical, economic, and predictive value, whatever its causes. Yes, correlation coefficients are noisy and have large error terms, but we have larger n of strains coming to the rescue. Expect more than 50 BXD lines soon.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>This is a thin veneer of functional genomics. It is enough to generate some marvelous hypotheses in a semi-automated way.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.41%;left:3.31%;width:105.43%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:292;mso-text-indent-alt: 0;position:absolute;top:0%;left:1.0%;width:98.99%;height:50.6%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>2.45 billion scatter plots: </span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>here is one of the best</span></div> </div> <img border=0 src="slide0012_image017.gif" style='position:absolute;top:11.3%; left:6.62%;width:67.41%;height:82.68%'> <div class=O style='position:absolute;top:77.56%;left:41.19%;width:8.6%; height:7.42%'><span style='font-family:"Gill Sans";font-size:283%;color:#003399; mso-color-index:4'><i>App</i></span><span style='font-family:"Gill Sans"; font-size:283%;color:#E9EB5D;mso-color-index:3;mso-special-format:lastCR; display:none'><i><br> </i></span></div> <img border=0 src="slide0012_image018.gif" alt="Text Box: Hsp84-1" style='position:absolute;top:36.04%;left:14.17%;width:6.88%;height:27.03%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>The correlation between App and heat shock protein 84-1 transcript is most impressive.<span style="mso-spacerun: yes">&nbsp; </span>Since WebQTL now contains total of about 70,000 traits in the BXD strains, we could produce as many as to 70k x 35k scatter plots of this type. Since all of the<span style="mso-spacerun: yes">&nbsp; </span>correlations come for a common reference population, none<span style="mso-spacerun: yes">&nbsp; </span>of the correlations are blantantly silly. However the great majority may be uninterpretable and a very large number may be meaningless given the signal-to-noise ratios of some measurements. With about 30 strains, correlations above 0.7 have a reasonably low false positive rate.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>The correlation between App and heat shock protein 84-1 transcript is most impressive.<span style="mso-spacerun: yes">&nbsp; </span>Since WebQTL now contains total of about 70,000 traits in the BXD strains, we could produce as many as to 70k x 35k scatter plots of this type. Since all of the<span style="mso-spacerun: yes">&nbsp; </span>correlations come for a common reference population, none<span style="mso-spacerun: yes">&nbsp; </span>of the correlations are blantantly silly. However the great majority may be uninterpretable and a very large number may be meaningless given the signal-to-noise ratios of some measurements. With about 30 strains, correlations above 0.7 have a reasonably low false positive rate.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.41%;left:3.31%;width:93.77%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:2.11%;width:96.46%;height:50.6%'><span style='mso-special-format:nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts"; font-size:99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index: 3'>Cross-tissue type correlations</span></div> </div> <img border=0 src="slide0013_image019.gif" style='position:absolute;top:11.83%; left:4.1%;width:71.52%;height:46.64%'><img border=0 src="slide0013_image020.gif" style='position:absolute;top:60.95%;left:4.1%;width:89.93%;height:35.33%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>We can compare App expression inthe forebrain against transcript expression in hematopoietic stem cells. Some of these correlations are significant, but it may be difficult to discovery of shared genetic (linkage disequilibrium) or molecular processes that give rise to these correlation.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>The GNF Hematopoietic stem cell data belong to Gerald de Haan (University of Groningen) and Michael Cooke (Genomics Institute of the Novartis Research Foundation).</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>We can compare App expression inthe forebrain against transcript expression in hematopoietic stem cells. Some of these correlations are significant, but it may be difficult to discovery of shared genetic (linkage disequilibrium) or molecular processes that give rise to these correlation.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>The GNF Hematopoietic stem cell data belong to Gerald de Haan (University of Groningen) and Michael Cooke (Genomics Institute of the Novartis Research Foundation).</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.41%;left:3.31%;width:107.81%;height:14.66%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:1.84%;width:98.15%;height:85.54%'><span style='position:absolute;top:0%;left:0%;width:99.37%'><span style='mso-special-format: nobullet;display:none;color:#FBFDB8;font-family:"Monotype Sorts";font-size: 99%'>l</span><span style='font-size:106%;color:#E9EB5D;mso-color-index:3'>Cross-modal correlations: From mRNA to to </span></span><span style='position:absolute; top:39.43%;left:4.75%;width:95.24%'><span style='font-size:106%;color:#E9EB5D; mso-color-index:3'>anatomy and to behavior and pharmacology</span></span></div> </div> <img border=0 src="slide0014_image021.gif" style='position:absolute;top:15.37%; left:6.62%;width:53.37%;height:34.62%'><img border=0 src="slide0014_image022.gif" style='position:absolute;top:46.81%;left:17.35%; width:65.16%;height:53.0%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Another example, but in this case we are generating correlations between variation in transcript levels with a database of approximately 430 published (and unpublished) phenotypes from BXD strains. Notice that the N of strains is variable (from 21 to 28 above). Rank order statistics is more appropriate when N is under 30.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>The Published Phenotypes database was prepared by Elissa Chesler and Robert Williams from data extracted from the literature or sent to us for inclusion by our colleagues. We especially thank John Crabbe (Oregon HSU) and Byron Jones (Pennsylvania SU) for providing us with large pre-compiled data tables.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Another example, but in this case we are generating correlations between variation in transcript levels with a database of approximately 430 published (and unpublished) phenotypes from BXD strains. Notice that the N of strains is variable (from 21 to 28 above). Rank order statistics is more appropriate when N is under 30.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>The Published Phenotypes database was prepared by Elissa Chesler and Robert Williams from data extracted from the literature or sent to us for inclusion by our colleagues. We especially thank John Crabbe (Oregon HSU) and Byron Jones (Pennsylvania SU) for providing us with large pre-compiled data tables.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:3.0%;left:2.11%;width:96.42%;height:19.61%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";mso-margin-left-alt:232; mso-text-indent-alt:0;position:absolute;top:0%;left:.96%;width:97.39%; height:100.0%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size:68%'><i>WebQTL<span style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp; </span><br> </i></span></span><span style='position: absolute;top:32.43%;left:4.37%;width:95.76%'><span style='font-size:68%'><i>link to <br> </i></span></span><span style='position:absolute;top:58.55%;left:4.37%; width:95.76%'><span style='font-size:86%'><i>www.webqtl.org/search.html</i></span><span style='font-size:68%;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <div style='position:absolute;top:36.57%;left:2.51%;width:73.24%;height:45.05%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=B style='position:absolute;top:0%;left:2.71%;width:97.28%; height:81.96%'> <div style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:0%;left:0%;width:94.6%;height:31.57%'><span style='position:absolute;top:0%;left:7.46%;width:92.53%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'><span style='mso-special-format: "numbullet3\,1";color:#FBFDB8;position:absolute;left:-8.06%;font-family:Times; font-size:99%'>1.</span></span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>Discovering shared expression </span></span><span style='position:absolute;top:43.93%;left:7.46%;width:82.12%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'>patterns<br> </span></span></div> <div style='mso-line-spacing:" 0 ";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:41.14%;left:0%;width:84.94%;height:31.57%'><span style='position:absolute;top:0%;left:8.31%;width:91.46%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'><span style='mso-special-format: "numbullet3\,2";color:#FBFDB8;position:absolute;left:-9.09%;font-family:Times; font-size:99%'>2.</span></span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>Discovering upstream </span></span><span style='position: absolute;top:43.93%;left:8.31%;width:91.46%'><span style='font-size:94%; color:#E9EB5D;mso-color-index:3'>modulators (QTLs)<br> </span></span></div> <div style='mso-line-spacing:" 0 &#1;";mso-margin-left-alt:348;mso-text-indent-alt: 56;position:absolute;top:82.29%;left:0%;width:100.0%;height:17.7%'><span style='position:absolute;top:0%;left:7.06%;width:92.75%'><span style='font-size:94%;color:#E9EB5D;mso-color-index:3'><span style='mso-special-format: "numbullet3\,3";color:#FBFDB8;position:absolute;left:-7.61%;font-family:Times; font-size:99%'>3.</span></span><span style='font-size:94%;color:#E9EB5D; mso-color-index:3'>Discovering downstream targets</span></span></div> </div> </div> <img border=0 src="slide0015_image023.gif" style='position:absolute;top:17.84%; left:74.17%;width:23.57%;height:64.31%'> <div class=O style='position:absolute;top:75.44%;left:74.56%;width:10.46%; height:14.48%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:42.68%'><span style='font-family:"Arial Rounded MT Bold";font-size:233%;color:#6E6E6E'>RNA</span><span style='font-family:"Arial Rounded MT Bold";font-size:300%;color:#6E6E6E; display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:45.12%;left:0%;width:82.27%;height:54.87%'><span style='font-family:"Arial Rounded MT Bold";font-size:300%;color:#6E6E6E; mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:82.15%;left:4.1%;width:96.42%;height:18.55%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'></div> <img border=0 src="slide0015_image024.gif" style='position:absolute;top:51.94%; left:47.28%;width:26.35%;height:10.77%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Part 2: Mapping upstream modulators or QTLs. A quantitative trait locus is a chromosomal region that harbors one or a few polymorphic gene loci that influence a trait. We are going to be looking for QTLs that modulate the steady state expression level of App in the adult mouse forebrain.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Part 2: Mapping upstream modulators or QTLs. A quantitative trait locus is a chromosomal region that harbors one or a few polymorphic gene loci that influence a trait. We are going to be looking for QTLs that modulate the steady state expression level of App in the adult mouse forebrain.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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display:none'><br> </span></div> </div> <div class=O style='position:absolute;top:92.93%;left:35.49%;width:29.53%; height:5.83%'> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.48%'><span style='font-family:Times;font-size:117%;color:white; display:none'><br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:51.51%;left:0%;width:100.0%; height:48.48%'><span style='font-family:Times;font-size:117%;color:white; mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:2.12%;left:-2.64%;width:105.82%;height:11.13%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:0%;left:0%;width:100.0%;height:88.88%'> <div style='text-align:center;mso-line-spacing:"&#1;%0 0 0";mso-margin-left-alt: 16;mso-text-indent-alt:16;position:absolute;top:0%;left:0%;width:100.0%; height:67.85%'><span style='font-family:Arial;font-size:267%;color:#E9EB5D'><i>How to make recombinant inbred strains (RI)</i></span><span style='font-family: Arial;font-size:150%;color:aqua;display:none'><br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1;%0 0 0";mso-margin-left-alt: 16;mso-text-indent-alt:16;position:absolute;top:60.71%;left:6.25%;width:87.48%; height:39.28%'><span style='font-family:Arial;font-size:150%;color:aqua; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0016_image025.gif" style='position:absolute;top:28.79%; left:45.43%;width:18.14%;height:1.06%'><img border=0 src="slide0016_image026.gif" style='position:absolute;top:20.31%;left:28.21%; width:1.98%;height:11.83%'> <div style='position:absolute;top:15.01%;left:25.03%;width:19.47%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.77%;left:6.8%;width:93.19%; height:78.94%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>C57BL/6J (B)<br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:83.21%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <img border=0 src="slide0016_image027.gif" style='position:absolute;top:20.31%; left:30.46%;width:1.98%;height:11.83%'> <div style='position:absolute;top:15.01%;left:62.25%;width:16.55%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.77%;left:8.0%;width:92.8%; height:78.94%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>DBA/2J (D)<br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:82.75%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <img border=0 src="slide0016_image028.gif" style='position:absolute;top:20.31%; left:66.62%;width:4.23%;height:11.83%'><img border=0 src="slide0016_image029.gif" style='position:absolute;top:35.51%;left:23.97%; width:50.86%;height:1.23%'><img border=0 src="slide0016_image030.gif" style='position:absolute;top:35.68%;left:24.1%;width:.66%;height:4.06%'><img border=0 src="slide0016_image031.gif" style='position:absolute;top:35.68%; left:40.92%;width:.52%;height:4.06%'><img border=0 src="slide0016_image032.gif" style='position:absolute;top:35.68%;left:57.61%;width:.52%;height:4.06%'><img border=0 src="slide0016_image033.gif" style='position:absolute;top:35.68%; left:74.3%;width:.52%;height:3.88%'> <div style='position:absolute;top:42.75%;left:5.69%;width:6.75%;height:12.72%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.33%;left:19.6%;width:70.58%; height:81.94%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F1<br> </b></span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.84%;left:0%;width:83.33%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format:lastCR; display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0016_image034.gif" style='position:absolute;top:39.92%; left:22.25%;width:4.23%;height:11.83%'><img border=0 src="slide0016_image035.gif" style='position:absolute;top:39.92%;left:38.94%; width:4.23%;height:11.83%'><img border=0 src="slide0016_image036.gif" style='position:absolute;top:39.92%;left:55.62%;width:4.23%;height:11.83%'><img border=0 src="slide0016_image037.gif" style='position:absolute;top:39.39%; left:72.84%;width:4.23%;height:11.83%'><img border=0 src="slide0016_image038.gif" style='position:absolute;top:43.46%;left:43.97%; width:11.12%;height:3.53%'><img border=0 src="slide0016_image039.gif" style='position:absolute;top:60.95%;left:20.66%;width:9.8%;height:3.53%'> <div style='position:absolute;top:71.73%;left:58.41%;width:20.52%;height:27.03%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:12.41%;left:6.45%;width:87.09%; height:82.35%'> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:69.84%'><span style='position:absolute;top:0%;left:0%;width:99.25%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'><b>20 generations </b></span></span><span style='position:absolute;top:39.77%;left:0%;width:100.0%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'><b>brother-sister </b></span></span><span style='position:absolute;top:80.68%;left:0%;width:100.0%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'><b>matings</b></span><span style='font-family:Arial;font-size:233%;color:#FFFF99;display:none'><b><br> </b></span></span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:73.8%;left:0%;width:100.0%; height:26.98%'><span style='font-family:Arial;font-size:233%;color:#FFFF99; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0016_image040.gif" style='position:absolute;top:60.95%; left:50.72%;width:9.93%;height:3.53%'><img border=0 src="slide0016_image041.gif" style='position:absolute;top:62.72%;left:54.03%;width:4.37%;height:28.97%'><img border=0 src="slide0016_image042.gif" style='position:absolute;top:61.13%; left:79.86%;width:10.86%;height:3.53%'><img border=0 src="slide0016_image043.gif" style='position:absolute;top:62.36%;left:83.97%; width:4.1%;height:28.79%'> <div style='position:absolute;top:92.93%;left:21.72%;width:9.4%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.77%;left:12.67%;width:84.5%; height:78.94%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD1</span><span style='font-family:Arial;font-size:150%;color:#484848;display:none'><br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:83.33%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#484848;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:92.75%;left:51.78%;width:9.4%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.77%;left:12.67%;width:84.5%; height:78.94%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD2<br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:83.33%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:92.93%;left:80.79%;width:10.99%;height:6.0%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:14.7%;left:12.04%;width:87.95%; height:64.7%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD80</span></div> </div> <div style='position:absolute;top:90.45%;left:64.1%;width:17.74%;height:17.84%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:5.94%;left:7.46%;width:92.53%; height:87.12%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.86%'><span style='font-family:Arial;font-size:300%;color:#FFFF99'>+ É +<br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.0%;left:0%;width:83.87%;height:48.86%'><span style='font-family:Arial;font-size:300%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:62.89%;left:4.9%;width:6.75%;height:12.72%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:6.94%;left:19.6%;width:70.58%; height:81.94%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F2<br> </b></span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.84%;left:0%;width:83.33%;height:49.15%'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format:lastCR; display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0016_image044.gif" style='position:absolute;top:54.24%; left:18.01%;width:75.62%;height:1.06%'><img border=0 src="slide0016_image045.gif" style='position:absolute;top:54.06%;left:17.88%; width:.66%;height:3.88%'><img border=0 src="slide0016_image046.gif" style='position:absolute;top:54.24%;left:32.45%;width:.52%;height:3.88%'><img border=0 src="slide0016_image047.gif" style='position:absolute;top:54.24%; left:48.07%;width:.52%;height:4.06%'><img border=0 src="slide0016_image048.gif" style='position:absolute;top:54.24%;left:62.78%;width:.52%;height:4.06%'><img border=0 src="slide0016_image049.gif" style='position:absolute;top:54.24%; left:77.74%;width:.52%;height:4.06%'><img border=0 src="slide0016_image050.gif" style='position:absolute;top:54.59%;left:92.71%;width:.52%;height:3.71%'><img border=0 src="slide0016_image051.gif" style='position:absolute;top:44.87%; left:49.13%;width:.79%;height:10.07%'><img border=0 src="slide0016_image052.gif" style='position:absolute;top:57.59%;left:15.89%;width:4.23%;height:11.83%'><img border=0 src="slide0016_image053.gif" style='position:absolute;top:57.59%; left:30.86%;width:4.37%;height:11.83%'><img border=0 src="slide0016_image054.gif" style='position:absolute;top:57.59%;left:46.35%; width:4.23%;height:11.83%'><img border=0 src="slide0016_image055.gif" style='position:absolute;top:57.59%;left:60.92%;width:4.23%;height:11.83%'><img border=0 src="slide0016_image056.gif" style='position:absolute;top:57.59%; left:75.62%;width:4.23%;height:11.83%'><img border=0 src="slide0016_image057.gif" style='position:absolute;top:57.59%;left:90.86%; width:4.23%;height:11.83%'><img border=0 src="slide0016_image058.gif" style='position:absolute;top:84.09%;left:56.42%;width:1.98%;height:1.41%'><img border=0 src="slide0016_image059.gif" style='position:absolute;top:80.21%; left:25.96%;width:1.98%;height:11.83%'><img border=0 src="slide0016_image060.gif" style='position:absolute;top:84.98%;left:86.09%; width:1.85%;height:2.12%'> <div style='position:absolute;top:75.61%;left:1.58%;width:14.17%;height:14.13%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:7.5%;left:.93%;width:97.19%; height:83.75%'> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:7.69%;width:83.65%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'><b>BXD RI<br> </b></span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:32.83%;left:0%;width:100.0%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'><b>Strain set<br> </b></span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:67.16%;left:7.69%;width:83.65%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> </div> <img border=0 src="slide0016_image061.gif" style='position:absolute;top:12.36%; left:14.17%;width:10.59%;height:6.36%'><img border=0 src="slide0016_image062.gif" style='position:absolute;top:10.6%;left:76.42%; width:14.3%;height:8.3%'> <div style='position:absolute;top:20.84%;left:17.48%;width:9.8%;height:14.13%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:7.5%;left:6.75%;width:86.48%; height:83.75%'> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:7.81%;width:82.81%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>fully<br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:32.83%;left:0%;width:100.0%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>inbred<br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:67.16%;left:7.81%;width:82.81%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:38.16%;left:2.91%;width:12.05%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.77%;left:3.29%;width:92.3%; height:78.94%'> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>isogenic<br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:51.11%;left:8.33%;width:83.33%; height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:54.41%;left:1.85%;width:12.45%;height:14.13%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:7.5%;left:2.12%;width:93.61%; height:83.75%'> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:7.95%;width:82.95%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>hetero-<br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:32.83%;left:0%;width:100.0%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>geneous<br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:67.16%;left:7.95%;width:82.95%; height:32.83%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:74.91%;left:29.0%;width:22.25%;height:22.08%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:4.0%;left:5.35%;width:88.09%; height:90.4%'> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:79.64%'><span style='position:absolute;top:0%;left:.67%;width:99.32%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>Recombined </span></span><span style='position:absolute;top:25.55%;left:.67%;width:99.32%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>chromosomes </span></span><span style='position:absolute;top:51.11%;left:0%;width:100.0%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>are needed for </span></span><span style='position:absolute;top:75.55%;left:.67%;width:99.32%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>mapping<br> </span></span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:80.53%;left:.67%;width:99.32%; height:19.46%'><span style='font-family:Arial;font-size:150%;color:#FFFF99; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:10.24%;left:25.56%;width:10.46%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.77%;left:12.65%;width:87.34%; height:78.94%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>female<br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:84.05%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:10.24%;left:64.5%;width:8.21%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:8.77%;left:14.51%;width:80.64%; height:78.94%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>male<br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:51.11%;left:0%;width:84.0%;height:48.88%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:30.56%;left:78.01%;width:19.47%;height:10.07%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:15.78%;left:6.12%;width:93.19%; height:71.92%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:41.46%'><span style='font-family:Arial;font-size:117%;color:#FFFF99'>chromosome pair</span><span style='font-family:Arial;font-size:150%;color:#FFFF99;display:none'><br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:46.34%;left:0%;width:83.21%;height:53.65%'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format:lastCR; display:none'><br> </span></div> </div> </div> <img border=0 src="slide0016_image063.gif" style='position:absolute;top:31.09%; left:65.82%;width:12.84%;height:2.82%'> <div style='position:absolute;top:86.21%;left:3.97%;width:10.06%;height:14.48%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:4.87%;left:5.26%;width:86.84%; height:85.36%'> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:0%;left:9.09%;width:83.33%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:#E9EB5D'>Inbred<br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:25.71%;left:0%;width:100.0%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:#E9EB5D'>Isogenic<br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:50.0%;left:6.06%;width:90.9%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:#E9EB5D'>siblings</span><span style='font-family:Arial;font-size:117%;color:aqua;display:none'><br> </span></div> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17;position:absolute;top:75.71%;left:9.09%;width:83.33%; height:24.28%'><span style='font-family:Arial;font-size:117%;color:aqua; mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0016_image064.gif" style='position:absolute;top:79.32%; left:83.7%;width:1.98%;height:11.83%'><img border=0 src="slide0016_image065.gif" style='position:absolute;top:79.85%;left:53.5%;width:1.98%;height:11.83%'><img border=0 src="slide0016_image066.gif" style='position:absolute;top:80.21%; left:23.44%;width:1.98%;height:11.83%'> <div style='position:absolute;top:18.72%;left:43.04%;width:13.77%;height:9.89%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:10.71%;left:9.61%;width:90.38%;height:76.78%'><span style='font-family:Arial;font-size:300%;color:aqua'>B</span><span style='font-family:Arial;font-size:300%;color:#FFFF99'>X</span><span style='font-family:Arial;font-size:300%;color:#FF1911'>D</span></div> </div> <img border=0 src="slide0016_image067.gif" style='position:absolute;top:27.2%; left:32.98%;width:31.39%;height:4.06%'><img border=0 src="slide0016_image068.gif" style='position:absolute;top:66.25%;left:60.92%; width:1.98%;height:3.18%'><img border=0 src="slide0016_image069.gif" style='position:absolute;top:64.31%;left:15.89%;width:1.98%;height:5.3%'><img border=0 src="slide0016_image070.gif" style='position:absolute;top:57.59%; left:75.62%;width:1.98%;height:5.65%'><img border=0 src="slide0016_image071.gif" style='position:absolute;top:28.44%;left:48.87%;width:.92%;height:8.12%'><img border=0 src="slide0016_image072.gif" style='position:absolute;top:62.19%; left:23.84%;width:3.57%;height:16.96%'><img border=0 src="slide0016_image073.gif" style='position:absolute;top:61.48%;left:63.17%; width:1.98%;height:8.12%'></div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The next set of slides provide a very short interlude on QTL mapping. You will need to do some independent reading on this topic if this is your first exposure to QTL mapping. The recombinant inbred strains that we are using in WebQTL and in this particular demo were generated about 25 years ago by Dr. Ben Taylor at The Jackson Laboratory. He crossed a female C57BL/6J mouse with a male DBA/2J mice. At the bottom of this slide we have schematized one chromosome pair from three out of 80 BXD RI strains.<span style="mso-spacerun: yes">&nbsp; </span>The dashed vertical lines that lead to the final BXD RI lines involve 20 full sib matings (about 6 years of breeding). Some lines die<span style="mso-spacerun: yes">&nbsp; </span>out during inbreeding. For example, there is no extant BXD3 or BXD4 strain.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The next set of slides provide a very short interlude on QTL mapping. You will need to do some independent reading on this topic if this is your first exposure to QTL mapping. The recombinant inbred strains that we are using in WebQTL and in this particular demo were generated about 25 years ago by Dr. Ben Taylor at The Jackson Laboratory. He crossed a female C57BL/6J mouse with a male DBA/2J mice. At the bottom of this slide we have schematized one chromosome pair from three out of 80 BXD RI strains.<span style="mso-spacerun: yes">&nbsp; </span>The dashed vertical lines that lead to the final BXD RI lines involve 20 full sib matings (about 6 years of breeding). Some lines die<span style="mso-spacerun: yes">&nbsp; </span>out during inbreeding. For example, there is no extant BXD3 or BXD4 strain.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'><img border=0 src="slide0017_image074.gif" style='position:absolute;top:40.45%; left:73.24%;width:14.56%;height:5.3%'> <div class=O style='position:absolute;top:40.81%;left:74.56%;width:11.92%; height:7.24%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white'>aa<br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:41.46%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white;mso-special-format:lastCR; display:none'><br> </span></div> </div> <img border=0 src="slide0017_image075.gif" style='position:absolute;top:16.6%; left:73.24%;width:14.56%;height:5.3%'> <div class=O style='position:absolute;top:16.96%;left:74.56%;width:11.92%; height:7.24%'> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white'>aaaa<br> </span></div> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:41.46%;left:0%;width:100.0%;height:60.97%'><span style='font-family:Gadget;font-size:150%;color:white;mso-special-format:lastCR; display:none'><br> </span></div> </div> <div style='position:absolute;top:20.31%;left:71.65%;width:25.96%;height:19.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.82%;left:5.1%;width:95.4%; height:83.03%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:#E2EBF0'>D2 strain</span><span style='font-family: Palatino;font-size:333%;color:#FBFDB8;display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:49.46%;left:0%;width:88.77%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#FBFDB8;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:43.81%;left:71.65%;width:25.56%;height:19.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:10.71%;left:5.18%;width:94.81%; height:83.03%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:#F6BF69'>B6 strain</span><span style='font-family: Palatino;font-size:333%;color:#F6BF69;display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:48.38%;left:0%;width:87.43%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#F6BF69;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0017_image076.gif" style='position:absolute;top:17.31%; left:31.25%;width:1.32%;height:42.22%'><img border=0 src="slide0017_image077.gif" style='position:absolute;top:40.63%;left:31.39%; width:39.86%;height:3.53%'> <div style='position:absolute;top:17.13%;left:40.0%;width:30.59%;height:31.44%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:11.23%;left:4.32%;width:90.9%; height:83.7%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:61.74%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size: 233%;color:#E9EB5D'><i>amount of </i></span></span><span style='position:absolute; top:59.78%;left:0%;width:100.0%'><span style='font-size:233%;color:#E9EB5D'><i>transcript</i></span><span style='font-family:Palatino;font-size:367%;color:#E9EB5D;display:none'><br> </span></span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:65.77%;left:0%;width:100.0%;height:34.89%'><span style='font-family:Palatino;font-size:367%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:6.0%;left:72.45%;width:21.32%;height:19.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:9.82%;left:6.21%;width:87.57%; height:83.03%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:white'>4 </span><span style='font-size:233%; color:white'>units</span><span style='font-family:Palatino;font-size:333%; color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:49.46%;left:0%;width:100.0%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:29.5%;left:72.45%;width:21.32%;height:19.78%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:10.71%;left:6.21%;width:87.57%; height:83.03%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:45.16%'><span style='font-size:267%;color:white'>2</span><span style='font-size:233%; color:white'> units</span><span style='font-family:Palatino;font-size:333%; color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:48.38%;left:0%;width:100.0%;height:50.53%'><span style='font-family:Palatino;font-size:333%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0017_image078.gif" style='position:absolute;top:15.72%; left:31.25%;width:40.26%;height:5.47%'> <div class=O style='position:absolute;top:17.84%;left:9.93%;width:4.76%; height:16.43%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:83.33%;height:50.53%'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div class=O style='position:absolute;top:17.84%;left:17.88%;width:4.23%; height:16.43%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:84.37%;height:50.53%'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:77.73%;left:5.43%;width:104.37%;height:26.32%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:8.72%;left:1.26%;width:98.73%; height:87.24%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:62.3%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size: 233%;color:#E2EBF0'>D</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%;color:#E9EB5D'>and </span><span style='font-size:233%; color:#F6BF69'>B</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%;color:#E9EB5D'>may be SNP-like variants in the promoter </span></span><span style='position:absolute;top:61.72%;left:0%;width:97.55%'><span style='font-size:200%;color:#E9EB5D'>itself (cis QTL) or in </span><span style='font-size:200%;color:#EAEC5E'>upstream genes (trans QTLs)</span><span style='font-size:300%;color:#E9EB5D;display:none'><br> </span></span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:63.84%;left:0%;width:86.88%;height:36.15%'><span style='font-size:300%;color:#E9EB5D;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div class=O style='position:absolute;top:7.59%;left:4.23%;width:22.78%; height:16.25%'> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:0%;left:1.74%;width:97.09%; height:33.69%'><span style='font-size:200%;color:#E9EB5D'>UPSTREAM<br> </span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:32.6%;left:0%;width:100.0%; height:33.69%'><span style='font-size:200%;color:#E9EB5D'>modulators<br> </span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:66.3%;left:4.65%;width:90.69%; height:33.69%'><span style='font-size:200%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:7.59%;left:44.5%;width:17.21%;height:18.19%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=O style='position:absolute;top:3.88%;left:6.92%;width:83.84%; height:90.29%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0'>High<br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:49.46%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:63.78%;left:8.74%;width:6.62%;height:18.19%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:3.88%;left:20.0%;width:72.0%; height:90.29%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:83.33%;height:50.53%'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:63.78%;left:15.09%;width:6.22%;height:18.19%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:3.88%;left:19.14%;width:68.08%; height:90.29%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.53%'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:49.46%;left:0%;width:84.37%;height:50.53%'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <div style='position:absolute;top:71.02%;left:7.54%;width:16.02%;height:12.72%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:5.55%;left:7.43%;width:91.73%; height:86.11%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.0%'><span style='font-size:200%;color:#E9EB5D'><i>cis QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:48.38%;left:0%;width:82.88%;height:50.0%'><span style='font-size:200%;color:#E9EB5D;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> <img border=0 src="slide0017_image079.gif" style='position:absolute;top:57.24%; left:-1.05%;width:101.98%;height:7.59%'> <div class=O style='position:absolute;top:44.16%;left:47.15%;width:9.4%; height:12.72%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:51.38%'><span style='font-size:233%;color:#F6BF69'>Low<br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:50.0%;left:0%;width:83.09%;height:51.38%'><span style='font-size:233%;color:#F6BF69;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div style='position:absolute;top:58.12%;left:.79%;width:114.56%;height:6.53%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:10.81%;left:1.15%;width:98.84%;height:72.97%'><span style='font-family:Gadget;font-size:167%;color:white'>&gt;&gt;&gt;&gt;PROMOTER--ATG-Exon1-Intron1-Exon2-Intron2 - etc-3'UTR &gt;&gt;&gt;&gt;&gt;</span></div> </div> <img border=0 src="slide0017_image080.gif" style='position:absolute;top:37.98%; left:8.6%;width:13.77%;height:20.67%'> <div class=O style='position:absolute;top:39.75%;left:10.06%;width:3.57%; height:12.01%'> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white'><b>&#4;<br> </b></span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:52.94%;left:7.4%;width:85.18%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <div class=O style='position:absolute;top:40.1%;left:17.48%;width:3.57%; height:12.01%'> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white'><b>&#4;<br> </b></span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:51.47%;left:7.4%;width:85.18%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <img border=0 src="slide0017_image081.gif" style='position:absolute;top:33.74%; left:8.6%;width:13.77%;height:20.67%'> <div class=O style='position:absolute;top:35.86%;left:17.48%;width:3.57%; height:12.01%'> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white'><b>&#4;<br> </b></span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:51.47%;left:7.4%;width:85.18%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <div class=O style='position:absolute;top:35.51%;left:10.06%;width:3.57%; height:12.01%'> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white'><b>&#4;<br> </b></span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:51.47%;left:7.4%;width:85.18%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <div class=O style='position:absolute;top:37.8%;left:20.66%;width:.13%; height:12.01%'> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:0%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; display:none'><b><br> </b></span></div> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;position:absolute;top:51.47%;left:0%;width:100.0%; height:48.52%'><span style='font-family:Palatino;font-size:233%;color:white; mso-special-format:lastCR;display:none'><b><br> </b></span></div> </div> <img border=0 src="slide0017_image082.gif" style='position:absolute;top:27.38%; left:4.9%;width:21.45%;height:21.37%'> <div style='position:absolute;top:27.38%;left:5.03%;width:21.05%;height:12.72%; filter:DropShadow(Color=#000000, OffX=1, OffY=1)'> <div class=O style='position:absolute;top:5.55%;left:6.28%;width:93.71%; height:86.11%'> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:0%;left:0%;width:100.0%;height:50.0%'><span style='font-size:200%;color:#E9EB5D'><i>trans QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;position:absolute;top:48.38%;left:0%;width:83.22%;height:50.0%'><span style='font-size:200%;color:#E9EB5D;mso-special-format:lastCR;display:none'><br> </span></div> </div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>This slide is illustrates two categories of QTLs that modulate variability in transcript abundance.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>1. cis QTLs are defined as QTLs that are closely linked to the gene whose transcript is the measured trait. For example, a polymorphism in the promoter that affects the binding of a transcription factor. However, cis QTLs can be far upstream or downstream polymorphisms in enhancers. They may also be polymorphisms in neigghboring genes.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. trans QTLs map far enough away from the location of the gene that gives rise to the transcript that is being measured so that we can be quite certain that the QTL is not IN the gene itself. The most blatant type of trans-QTL would be a polymorphism in a transcription factor. BUT in the majority of cases, the trans QTLs can be far removed in a mechanistic sense from the actual events modulating transcript abundance. That is why there are three overlappoing arrows above.<span style="mso-spacerun: yes">&nbsp; </span>The way in which an upstream polymorphism influences a downstream difference in mRNA abundance can be very indirect. Effects can :</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>a.<span style="mso-spacerun: yes">&nbsp; </span>cross tissue types (a polymorphic liver enzyme may affect CNS gene expression)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>b.<span style="mso-spacerun: yes">&nbsp; </span>cross time (the modulator is only expressed for one day during development but has permanent effects in adults),</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>c.<span style="mso-spacerun: yes">&nbsp; </span>may be contingent on environmental factors (heat shock may trigger the expression of a polymorphic factor that affects mRNA abundance).</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>This slide is illustrates two categories of QTLs that modulate variability in transcript abundance.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>1. cis QTLs are defined as QTLs that are closely linked to the gene whose transcript is the measured trait. For example, a polymorphism in the promoter that affects the binding of a transcription factor. However, cis QTLs can be far upstream or downstream polymorphisms in enhancers. They may also be polymorphisms in neigghboring genes.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>2. trans QTLs map far enough away from the location of the gene that gives rise to the transcript that is being measured so that we can be quite certain that the QTL is not IN the gene itself. The most blatant type of trans-QTL would be a polymorphism in a transcription factor. BUT in the majority of cases, the trans QTLs can be far removed in a mechanistic sense from the actual events modulating transcript abundance. That is why there are three overlappoing arrows above.<span style="mso-spacerun: yes">&nbsp; </span>The way in which an upstream polymorphism influences a downstream difference in mRNA abundance can be very indirect. Effects can :</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>a.<span style="mso-spacerun: yes">&nbsp; </span>cross tissue types (a polymorphic liver enzyme may affect CNS gene expression)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>b.<span style="mso-spacerun: yes">&nbsp; </span>cross time (the modulator is only expressed for one day during development but has permanent effects in adults),</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2><span style="mso-spacerun: yes">&nbsp;&nbsp; </span>c.<span style="mso-spacerun: yes">&nbsp; </span>may be contingent on environmental factors (heat shock may trigger the expression of a polymorphic factor that affects mRNA abundance).</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:2.29%;left:4.1%;width:105.16%;height:9.01%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";position:absolute;top:11.76%; left:1.25%;width:98.86%;height:82.35%'><span style='font-family:Verdana; font-size:73%'><i>WebQTL to exploring upstream control</i></span></div> </div> <img border=0 src="slide0018_image083.gif" style='position:absolute;top:13.07%; left:5.16%;width:89.66%;height:62.19%'><img border=0 src="slide0018_image084.gif" style='position:absolute;top:19.25%;left:55.49%; width:19.2%;height:25.97%'> <div class=O style='position:absolute;top:16.43%;left:74.3%;width:15.49%; height:5.65%'><span style='font-family:"Gill Sans";font-size:217%;color:#FF0F0F'>Just click</span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Back to the demo. Please bring the Traiit Data and Editing window to the front and look for the Interval Mapping button. Please confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Probes is Probe Set</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Permuation test YES<span style="mso-spacerun: yes">&nbsp; </span>(1000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Bootstrap test YES (1000 is the default number)</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Back to the demo. Please bring the Traiit Data and Editing window to the front and look for the Interval Mapping button. Please confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Select Probes is Probe Set</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Options: Permuation test YES<span style="mso-spacerun: yes">&nbsp; </span>(1000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Options: Bootstrap test YES (1000 is the default number)</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:2.29%;left:4.1%;width:106.88%;height:9.01%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";mso-margin-left-alt:233; mso-text-indent-alt:0;position:absolute;top:11.76%;left:.99%;width:99.0%; height:82.35%'><span style='font-family:Verdana;font-size:73%'><i>WebQTL to exploring upstream control.</i></span></div> </div> <img border=0 src="slide0019_image085.gif" style='position:absolute;top:27.2%; left:1.72%;width:96.68%;height:51.76%'> <div class=O style='position:absolute;top:13.78%;left:19.33%;width:60.52%; height:6.53%'><span style='font-size:233%;color:#E9EB5D'><i>App maps on Chr 16 here</i></span></div> <img border=0 src="slide0019_image086.gif" style='position:absolute;top:15.9%; left:68.34%;width:14.43%;height:37.98%'><img border=0 src="slide0019_image087.gif" style='position:absolute;top:53.18%;left:13.5%; width:14.43%;height:37.98%'> <div class=O style='position:absolute;top:82.86%;left:26.88%;width:90.59%; height:14.48%'> <div style='position:absolute;top:0%;left:0%;width:83.33%;height:51.21%'><span style='font-size:267%;color:#E9EB5D'><i>Is App modulated by Chr 2?<br> </i></span></div> <div style='position:absolute;top:50.0%;left:0%;width:100.0%;height:51.21%'><span style='font-size:267%;color:#E9EB5D'><i>Probably, but donÕt bet the farm. </i></span></div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This is the main output type: a so-called full genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromsomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans and almost 0 megabases). The blue labels along the bottom of the figure list a subset of markers that were used in mapping. We used 753 markers to perform the mapping but here we just list five markers per chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The thick blue wavy line running across chromsomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of 753 markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The red line and the red axis to the far right provides an estimate of the effect<span style="mso-spacerun: yes">&nbsp; </span>that a QTL has on expression of App (this estimate of the addtive effect tends to be an overestimate). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher traits. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and the D/D genotype at a specific marker. For example, on Chr 2 the red line<span style="mso-spacerun: yes">&nbsp; </span>peaks at a value<span style="mso-spacerun: yes">&nbsp; </span>of about 0.25. That means that this region of chromosome 2 is responsible for a 0.5 unit expression difference between B/B strains and the D/D strains. Since the units are log base 2, this is 2^0.5, or about a 41% difference in expression with the D/D group being high.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides you a metho of evaluating whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But inthe bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 2 under the LRS peak. That is somewhat reassuring. But notice that a substantial number of bootstrap results prefer Chr 7 or Chr 18.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The horizontal dashed lines at 9.6 and 15.9. These lines are the LRS values associated with the suggestive and significant false positive rates for genome-wide scans established by permuations of phenotypes across genotypes. We shuffle randomly 1000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 15.9. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 2 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations.<span style="mso-spacerun: yes">&nbsp; </span>Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. If you click on the body of the map, say on the blue line, then you will generate a view<span style="mso-spacerun: yes">&nbsp; </span>on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. If you click on a marker symbol, then you will generate a new Trait data and editing window with the genotypes loaded into the window just like any other trait. More on this later.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>NOTE: you can drag these maps off of the browser window and onto your desktop. The will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>This is the main output type: a so-called full genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromsomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans and almost 0 megabases). The blue labels along the bottom of the figure list a subset of markers that were used in mapping. We used 753 markers to perform the mapping but here we just list five markers per chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The thick blue wavy line running across chromsomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of 753 markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The red line and the red axis to the far right provides an estimate of the effect<span style="mso-spacerun: yes">&nbsp; </span>that a QTL has on expression of App (this estimate of the addtive effect tends to be an overestimate). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher traits. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and the D/D genotype at a specific marker. For example, on Chr 2 the red line<span style="mso-spacerun: yes">&nbsp; </span>peaks at a value<span style="mso-spacerun: yes">&nbsp; </span>of about 0.25. That means that this region of chromosome 2 is responsible for a 0.5 unit expression difference between B/B strains and the D/D strains. Since the units are log base 2, this is 2^0.5, or about a 41% difference in expression with the D/D group being high.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides you a metho of evaluating whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But inthe bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 2 under the LRS peak. That is somewhat reassuring. But notice that a substantial number of bootstrap results prefer Chr 7 or Chr 18.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The horizontal dashed lines at 9.6 and 15.9. These lines are the LRS values associated with the suggestive and significant false positive rates for genome-wide scans established by permuations of phenotypes across genotypes. We shuffle randomly 1000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 15.9. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 2 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations.<span style="mso-spacerun: yes">&nbsp; </span>Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. If you click on the body of the map, say on the blue line, then you will generate a view<span style="mso-spacerun: yes">&nbsp; </span>on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>3. If you click on a marker symbol, then you will generate a new Trait data and editing window with the genotypes loaded into the window just like any other trait. More on this later.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>NOTE: you can drag these maps off of the browser window and onto your desktop. The will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>App has a suggestive QTL on Chr 2. What about the neighbors that we defined as having shared expression patterns. This figure shows that members of the immediate App neigborhood share a weak Chr 2 QTL.<span style="mso-spacerun: yes">&nbsp; </span>That is what the blue oval in the background is meant to represent. But some transcripts, such as Ndr4 and Psen2 do not share this Chr 2 interval.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>QUESTION: What kind of headway can we make in detemining what polymorphism or polymorphisms on Chr 2 near 130 Mb might contribute to the variance in the expression of all of these important transcripts?</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:2.29%;left:4.1%;width:93.9%;height:9.01%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";position:absolute;top:11.76%; left:1.41%;width:97.03%;height:82.35%'><span style='font-family:Verdana; font-size:73%'><i>Which gene is the QTL?</i></span></div> </div> <img border=0 src="slide0021_image089.gif" style='position:absolute;top:13.07%; left:5.03%;width:58.27%;height:37.98%'><img border=0 src="slide0021_image090.gif" style='position:absolute;top:46.81%;left:3.31%; width:68.87%;height:51.94%'><img border=0 src="slide0021_image091.gif" style='position:absolute;top:38.51%;left:55.62%;width:24.76%;height:25.08%'> <div class=O style='position:absolute;top:7.77%;left:75.09%;width:21.05%; height:28.97%'> <div style='text-align:center;position:absolute;top:0%;left:8.17%;width:83.64%; height:25.6%'><span style='font-size:267%;color:#E9EB5D'><i>Right <br> </i></span></div> <div style='text-align:center;position:absolute;top:25.0%;left:0%;width:100.0%; height:25.6%'><span style='font-size:267%;color:#E9EB5D'><i>position<br> </i></span></div> <div style='text-align:center;position:absolute;top:50.0%;left:8.17%; width:83.64%;height:25.6%'><span style='font-size:267%;color:#E9EB5D'><i>&amp;<br> </i></span></div> <div style='text-align:center;position:absolute;top:75.0%;left:8.17%; width:83.64%;height:25.6%'><span style='font-size:267%;color:#E9EB5D'><i>high r</i></span></div> </div> <div class=O style='position:absolute;top:50.7%;left:72.58%;width:28.87%; height:14.48%'> <div style='text-align:center;position:absolute;top:0%;left:8.71%;width:83.48%; height:51.21%'><span style='font-size:267%;color:#E9EB5D'><i>good<br> </i></span></div> <div style='text-align:center;position:absolute;top:50.0%;left:0%;width:100.0%; height:51.21%'><span style='font-size:267%;color:#E9EB5D'><i>candidates</i></span></div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Candidate Genes: </b><span style="mso-spacerun: yes">&nbsp;</span>The best we can do at this point is to make an educated guess about the candidacy status of all genes in the QTL support interval. For sake of argument, lets say that we are confident that the polymorphism is located between 130 and 150 Mb (20 Mb, equivalent to roughly 10 cM). There will typically be 12 to 15 genes per Mb, so we now would like to evaluate 240 to 300 positional candidates. We would like to highlight the biologically relevant subset of candidates. We could look through gene ontologies and expression levels to help us winnow the list. An alternate way avaiable using WebQTL is to generate a list of those genes in this 20 Mb interval that have transcripts that co-vary in expression with App expression.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>To do this, go back to the Trait Data and Editing window. Sort the correlations by position. Select Return = 500. Then scroll down the list to see positional candidates that share expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>There are several candidates that have high correlation with App even in this short 20 Mb interval. We can rank them by correlation, but they are all close.<span style="mso-spacerun: yes">&nbsp; </span>There is one other imporant approach to ranking these candidates. Are they likely to contain polymorphisms? We can assess the likelihood that they contain polymorphisms by mapping each transcript to see if any have strong cis QTLs. The logic of this search is that a transcript that has a strong cis-QTL is likely to contain functional polymorphisms that effect its own expression. This make is more like that the transcript is a ÒcausativeÓ factor since it is likely to be polymorphic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Candidate Genes: </b><span style="mso-spacerun: yes">&nbsp;</span>The best we can do at this point is to make an educated guess about the candidacy status of all genes in the QTL support interval. For sake of argument, lets say that we are confident that the polymorphism is located between 130 and 150 Mb (20 Mb, equivalent to roughly 10 cM). There will typically be 12 to 15 genes per Mb, so we now would like to evaluate 240 to 300 positional candidates. We would like to highlight the biologically relevant subset of candidates. We could look through gene ontologies and expression levels to help us winnow the list. An alternate way avaiable using WebQTL is to generate a list of those genes in this 20 Mb interval that have transcripts that co-vary in expression with App expression.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>To do this, go back to the Trait Data and Editing window. Sort the correlations by position. Select Return = 500. Then scroll down the list to see positional candidates that share expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>There are several candidates that have high correlation with App even in this short 20 Mb interval. We can rank them by correlation, but they are all close.<span style="mso-spacerun: yes">&nbsp; </span>There is one other imporant approach to ranking these candidates. Are they likely to contain polymorphisms? We can assess the likelihood that they contain polymorphisms by mapping each transcript to see if any have strong cis QTLs. The logic of this search is that a transcript that has a strong cis-QTL is likely to contain functional polymorphisms that effect its own expression. This make is more like that the transcript is a ÒcausativeÓ factor since it is likely to be polymorphic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-<html> <head> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <title>PowerPoint Presentation - Complex trait analysis, develop-ment, and genomics</title> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.59%;left:3.97%;width:104.1%;height:13.78%; filter:DropShadow(Color=#000000, OffX=4, OffY=4)'> <div class=O style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:1.28%;left:1.27%;width:98.72%;height:88.46%'><span style='position:absolute;top:0%;left:0%;width:100.0%'><span style='font-size: 233%;color:#E9EB5D'><i>Only one of these candidates is a functional </i></span></span><span style='position:absolute;top:46.37%;left:0%;width:85.69%'><span style='font-size:233%;color:#E9EB5D'><i>polymorphism</i></span></span></div> </div> <img border=0 src="slide0022_image092.gif" style='position:absolute;top:15.54%; left:2.64%;width:41.98%;height:84.45%'><img border=0 src="slide0022_image093.gif" style='position:absolute;top:31.44%;left:26.09%; width:27.15%;height:5.83%'> <div class=O style='position:absolute;top:28.09%;left:54.3%;width:50.06%; height:21.73%'> <div style='position:absolute;top:0%;left:0%;width:100.0%;height:25.2%'><span style='font-size:200%;color:#E9EB5D'><i>Hars2 = 0610006H08Rik<br> </i></span></div> <span style='position:absolute;top:24.39%;left:0%;width:94.7%'><span style='font-size:200%;color:#E9EB5D'><i>is a cis-QTL with a very </i></span></span><span style='position:absolute;top:49.59%;left:0%;width:84.39%'><span style='font-size:200%;color:#E9EB5D'><i>high likelihood ratio </i></span></span><span style='position:absolute;top:73.98%;left:0%;width:84.39%'><span style='font-size:200%;color:#E9EB5D'><i>statistic (LRS) score</i></span><span style='font-size:267%;color:#E9EB5D;mso-special-format:lastCR;display:none'><i><br> </i></span></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>When you do this you will find that only the transcript 0610006H08Rik has a strong cis QTL. See the slide above. The LRS peaks above 35<span style="mso-spacerun: yes">&nbsp; </span>(LOD of greater than 7.5). It turns out that this transcript is really Hars2, also known as histydl tRNA synthase 2.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>When you do this you will find that only the transcript 0610006H08Rik has a strong cis QTL. See the slide above. The LRS peaks above 35<span style="mso-spacerun: yes">&nbsp; </span>(LOD of greater than 7.5). It turns out that this transcript is really Hars2, also known as histydl tRNA synthase 2.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:1.59%;left:3.97%;width:104.1%;height:8.12%; filter:DropShadow(Color=#000000, OffX=4, OffY=4)'> <div class=O style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16;position:absolute;top:2.17%;left:1.27%;width:98.85%;height:80.43%'><span style='font-size:233%;color:#E9EB5D'><i>Hars2 probe level analysis: 16 PMs mapped</i></span></div> </div> <img border=0 src="slide0023_image094.gif" style='position:absolute;top:10.77%; left:13.64%;width:72.58%;height:84.8%'><img border=0 src="slide0023_image095.gif" style='position:absolute;top:59.18%;left:2.38%; width:41.05%;height:36.74%'><img border=0 src="slide0023_image096.gif" style='position:absolute;top:60.07%;left:36.82%;width:26.75%;height:19.08%'><img border=0 src="slide0023_image097.gif" style='position:absolute;top:33.39%; left:21.19%;width:64.37%;height:2.65%'><img border=0 src="slide0023_image098.gif" style='position:absolute;top:35.68%;left:21.19%; width:64.37%;height:2.65%'><img border=0 src="slide0023_image099.gif" style='position:absolute;top:38.33%;left:21.19%;width:64.37%;height:2.65%'><img border=0 src="slide0023_image100.gif" style='position:absolute;top:19.78%; left:20.79%;width:28.74%;height:21.73%'> <div class=O style='position:absolute;top:28.09%;left:30.06%;width:4.9%; height:3.35%'><span style='font-size:117%;color:#FF0F0F'><i>SNP</i></span></div> <div class=O style='mso-line-spacing:"100 50 0";position:absolute;top:29.15%; left:40.13%;width:18.67%;height:3.35%'><span style='font-size:117%;color:#FF0F0F'>C in B6, T in D2 </span><span style='font-size:150%;color:black;mso-special-format: lastCR;display:none'><b><br> </b></span></div> <div class=O style='position:absolute;top:36.39%;left:50.99%;width:9.8%; height:3.35%'><span style='font-size:117%;color:#003399;mso-color-index:4'><i>no SNPs</i></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>LetÕs look at Hars2 in more detail by mapping all of the perfect match probes (16 of them) that target this transcript.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Go back to the Trait Data and Editing window and select Chr 2 (rather than ALL as shown above) and also select PM Probes. Then click on Interval Mapping button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>You will get the illustration above, but without the sequence data that we have added.<span style="mso-spacerun: yes">&nbsp; </span>The 16 perfect match probes are arranged in sequence (red is 5 prime, blue is the 3 prime end). For example, the 5 prime-most primer 307387 has the sequence CACTG..... It also has a polymorphism at the 17 nucleotide of this 25 nt probe sequence.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>How do we know that the 5 prime probe is polymorphic? By looking up the sequence in the Celera Genomics databases which often contqains sequence data for C57BL/6J (B6 above) and for DBA/2J.<span style="mso-spacerun: yes">&nbsp; </span>But two blue probes (14 and 15) do NOT contain SNPs but still have very large LRS scores. The other probes do not perform so wel. Highly variable probe performance is probably a result of the very different stacking energies of DNA-RNA duplexes.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
-if ( ! top.PPTPRESENTATION ) {
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>LetÕs look at Hars2 in more detail by mapping all of the perfect match probes (16 of them) that target this transcript.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Go back to the Trait Data and Editing window and select Chr 2 (rather than ALL as shown above) and also select PM Probes. Then click on Interval Mapping button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>You will get the illustration above, but without the sequence data that we have added.<span style="mso-spacerun: yes">&nbsp; </span>The 16 perfect match probes are arranged in sequence (red is 5 prime, blue is the 3 prime end). For example, the 5 prime-most primer 307387 has the sequence CACTG..... It also has a polymorphism at the 17 nucleotide of this 25 nt probe sequence.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>How do we know that the 5 prime probe is polymorphic? By looking up the sequence in the Celera Genomics databases which often contqains sequence data for C57BL/6J (B6 above) and for DBA/2J.<span style="mso-spacerun: yes">&nbsp; </span>But two blue probes (14 and 15) do NOT contain SNPs but still have very large LRS scores. The other probes do not perform so wel. Highly variable probe performance is probably a result of the very different stacking energies of DNA-RNA duplexes.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-3.53%;left:2.11%;width:96.42%;height:18.55%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";mso-margin-left-alt:232; mso-text-indent-alt:0;position:absolute;top:30.47%;left:.96%;width:97.39%; height:35.23%'><span style='font-size:68%'><i>Is there known biology to link Hars2 with App? </i></span></div> </div> <div style='position:absolute;top:82.15%;left:4.1%;width:96.42%;height:18.55%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'></div> <div class=O style='position:absolute;top:62.54%;left:5.16%;width:29.13%; height:12.36%'> <div style='position:absolute;top:0%;left:0%;width:100.0%;height:34.28%'><span style='font-size:150%;color:#E9EB5D;mso-color-index:3'><b>69 SNPs, 1 cSNP: <br> </b></span></div> <div style='position:absolute;top:32.85%;left:0%;width:96.81%;height:34.28%'><span style='font-size:150%;color:#E9EB5D;mso-color-index:3'><b>6 exons in NCBI, <br> </b></span></div> <div style='position:absolute;top:65.71%;left:0%;width:100.0%;height:34.28%'><span style='font-size:150%;color:#E9EB5D;mso-color-index:3'><b>2 exons in Celera</b></span></div> </div> <img border=0 src="slide0025_image105.gif" style='position:absolute;top:9.54%; left:3.31%;width:60.79%;height:42.57%'><img border=0 src="slide0025_image106.gif" style='position:absolute;top:28.62%;left:40.0%; width:55.76%;height:69.78%'> <div class=O style='position:absolute;top:14.13%;left:69.4%;width:23.84%; height:6.53%'><span style='font-family:"Gill Sans";font-size:250%;color:#E9EB5D'><i><span style="mso-spacerun: yes">&nbsp;</span>Not obvious</i></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Hars2 is not a well characterized gene and their is no biology yet to support the hypothesis that Hars2 modulates gene expression, let alone App expression in specific. There are also serious database/assembly discrepancies between Celera and MGSCv3 regarding the genomic organization of this gene. But there appear to be approximately 69 SNPs in Hars2, one of which results in a substitution.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>Part 3.<span style="mso-spacerun: yes">&nbsp; </span>Many investigators would like to discover the set of downstream targets of a gene of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=2>In a genetic and functional sense, that question can only be addressed effectively if there is genetic variation in the particular gene.<span style="mso-spacerun: yes">&nbsp; </span>We know that Fos is an important transcription factor, but unless it is polymorphic between C57BL/6J and DBA/2J, then it cannot generate a genetic variance signal with which we can work. We can still study covariance of Fos and hundreds of other transcripts (an interesting exercise), but there are no genetic causes-and-effects.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:2.29%;left:2.51%;width:108.47%;height:18.9%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";position:absolute;top:7.47%; left:1.22%;width:98.77%;height:85.04%'><span style='position:absolute; top:0%;left:0%;width:100.0%'><span style='font-size:82%'><i>Requirement: The gene must be polymorphic to </i></span></span><span style='position:absolute; top:51.64%;left:3.7%;width:79.72%'><span style='font-size:82%'><i>be genetically ÒupstreamÓ</i></span><span style='font-size:82%;mso-special-format: lastCR;display:none'><br> </span></span></div> </div> <div style='position:absolute;top:21.37%;left:2.51%;width:84.5%;height:15.01%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";position:absolute;top:32.94%; left:1.56%;width:98.43%;height:40.0%'><span style='font-size:64%'><i>What are targets of the Hars2 polymorphisms?</i></span><span style='font-size:82%; mso-special-format:lastCR;display:none'><br> </span></div> </div> <img border=0 src="slide0027_image108.gif" style='position:absolute;top:33.92%; left:1.72%;width:49.66%;height:32.15%'><img border=0 src="slide0027_image109.gif" style='position:absolute;top:33.39%;left:53.37%; width:44.23%;height:58.65%'> <div class=O style='position:absolute;top:69.96%;left:7.54%;width:51.39%; height:21.2%'> <div style='position:absolute;top:0%;left:0%;width:84.02%;height:32.5%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>App and many other<br> </i></span></div> <span style='position:absolute;top:33.33%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>correlated transcripts and </i></span></span><span style='position:absolute;top:67.5%; left:0%;width:84.02%'><span style='font-family:"Gill Sans";font-size:267%; color:#E9EB5D'><i>other traits.</i></span></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Genes must be polymorphic to generate downstream genetic effects (as opposed to downstream molecular effects). Hars2 meets this condition because we have already mapped a functional polymorphism in the gene. We can therefore posit that Hars2 is a QT gene. What transcripts are downstream? <i>App</i> is one obvious candidate, but there are many more.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The are several ways to look for downstream targets. The best and most obvious is to look up all transcripts that have high correlations with Hars2 itself. You should know how to do this. An alternative method is shown here for teaching purpose and to show you what to do if your gene of interest is not in our database. You need to know:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Times size=3>1.</font><font face=Verdana size=3> Where your gene is located. You need this information to find a surrogate marker; a marker that is located very close to your gene of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. That your gene is polymorphic between C57BL/6J and DBA/2J.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>LetÕs look at the correlation of Hars2 with BXD genotypes as shown in the slide above to illustrate how to use markers as surrogate traits.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Go to the Trait Date and Editing window one more time. We want the data for Hars2 this time, not App. You should be able to show that Hars2 has a high<span style="mso-spacerun: yes">&nbsp; </span>correlation with D2Mit423 as shown in the slide above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>By clicking on the symbol D2Mit423 in the Correlation window, you will generate a new Trait Data window shown on the next slide.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Genes must be polymorphic to generate downstream genetic effects (as opposed to downstream molecular effects). Hars2 meets this condition because we have already mapped a functional polymorphism in the gene. We can therefore posit that Hars2 is a QT gene. What transcripts are downstream? <i>App</i> is one obvious candidate, but there are many more.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>The are several ways to look for downstream targets. The best and most obvious is to look up all transcripts that have high correlations with Hars2 itself. You should know how to do this. An alternative method is shown here for teaching purpose and to show you what to do if your gene of interest is not in our database. You need to know:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Times size=2>1.</font><font face=Verdana size=2> Where your gene is located. You need this information to find a surrogate marker; a marker that is located very close to your gene of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>2. That your gene is polymorphic between C57BL/6J and DBA/2J.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>LetÕs look at the correlation of Hars2 with BXD genotypes as shown in the slide above to illustrate how to use markers as surrogate traits.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>Go to the Trait Date and Editing window one more time. We want the data for Hars2 this time, not App. You should be able to show that Hars2 has a high<span style="mso-spacerun: yes">&nbsp; </span>correlation with D2Mit423 as shown in the slide above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>By clicking on the symbol D2Mit423 in the Correlation window, you will generate a new Trait Data window shown on the next slide.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-1.23%;left:2.51%;width:110.06%;height:11.66%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";position:absolute;top:16.66%; left:1.2%;width:98.79%;height:66.66%'><span style='font-size:82%'><i>Direct correlations between genotypes and traits</i></span><span style='font-size: 82%;mso-special-format:lastCR;display:none'><br> </span></div> </div> <div class=O style='position:absolute;top:22.43%;left:3.44%;width:34.83%; height:42.4%'> <div style='position:absolute;top:0%;left:0%;width:100.0%;height:16.25%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>App and<br> </i></span></div> <span style='position:absolute;top:16.66%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>correlated traits </i></span></span><span style='position:absolute;top:33.33%;left:0%; width:100.0%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>would be obvious </i></span></span><span style='position:absolute;top:50.0%; left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:267%; color:#E9EB5D'><i>candidates to </i></span></span><span style='position:absolute; top:67.08%;left:0%;width:100.0%'><span style='font-family:"Gill Sans"; font-size:267%;color:#E9EB5D'><i>correlate with </i></span></span><span style='position:absolute;top:83.75%;left:0%;width:100.0%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>D2Mit423</i></span></span></div> <img border=0 src="slide0028_image110.gif" style='position:absolute;top:10.07%; left:33.37%;width:40.0%;height:87.63%'><img border=0 src="slide0028_image111.gif" style='position:absolute;top:82.68%;left:65.29%; width:14.7%;height:7.24%'> <div class=O style='position:absolute;top:79.68%;left:82.11%;width:13.11%; height:13.95%'> <div style='position:absolute;top:0%;left:0%;width:100.0%;height:49.36%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>B = -1<br> </i></span></div> <div style='position:absolute;top:50.63%;left:0%;width:94.94%;height:49.36%'><span style='font-family:"Gill Sans";font-size:267%;color:#E9EB5D'><i>D = 1</i></span></div> </div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>We can review the set of correlations between the marker D2Mit423 and all transcripts in forebrain.<span style="mso-spacerun: yes">&nbsp; </span>This is in essence a backwards way of mapping QTLs. We are considering one marker and asking what traits correlate to the marker and how well.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>We can review the set of correlations between the marker D2Mit423 and all transcripts in forebrain.<span style="mso-spacerun: yes">&nbsp; </span>This is in essence a backwards way of mapping QTLs. We are considering one marker and asking what traits correlate to the marker and how well.</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body style='margin:0px;background-color:black' onclick="DocumentOnClick(event)" onresize="_RSW()" onkeypress="_KPH(event)"> <div id=SlideObj class=sld style='position:absolute;top:0px;left:0px; width:755px;height:566px;font-size:16px;background-color:#484848;clip:rect(0%, 101%, 101%, 0%); visibility:hidden'><img src="master03_background.gif" v:shapes="_x0000_s1026" style='position:absolute;top:0%;left:0%;width:100.0%;height:100.0%'> <div style='position:absolute;top:-.35%;left:2.51%;width:96.95%;height:11.66%; filter:DropShadow(Color=#000000, OffX=2, OffY=2)'> <div class=T style='mso-line-spacing:" 0 &#1;";position:absolute;top:16.66%; left:1.36%;width:98.77%;height:66.66%'><span style='font-size:82%'><i>WhatÕs downstream of Chr 2 near Hars2?</i></span><span style='font-size:82%; mso-special-format:lastCR;display:none'><br> </span></div> </div> <img border=0 src="slide0030_image115.gif" style='position:absolute;top:12.19%; left:7.54%;width:61.58%;height:75.61%'> <div class=O style='position:absolute;top:90.1%;left:5.16%;width:107.01%; height:6.0%'><span style='font-family:"Gill Sans";font-size:233%;color:#E9EB5D'><i>modest support that Actn2 is modulated by the Hars2 region</i></span></div> </div> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>There is some support for the hypothesis that Actn2 is downstream of a polymorphism in the Hars2 region. But again, due to the 10 to 20 Mb precision of the mapping data, this relation could be generated by a large number of other polymorphisms close to Hars2. In the absence of a biological connection between Actn2 and Hars2 we have a weak hypothesis. If there were a plausible functional connection between the two genes, then this hypothesis could be quickly upgraded.</font><br> </td> </tr> </table> </div> <script language=JavaScript><!--
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-<html> <head> <meta name=ProgId content=PowerPoint.Slide> <meta name=Generator content="Microsoft Macintosh PowerPoint 10"> <link id=Main-File rel=Main-File href="WebQTLDemo.htm"> <link title="Presentation File" type="application/powerpoint" rel=alternate href=WebQTLDemo.ppt> <script>
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-</script> </head> <body bgcolor=black text=white> <table border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>We can test the Hars2 to Actn2 connection directly. This process weakens the putative association. We are ready to move on and examine other candidates in the Hars2 region near D2Mit423.<span style="mso-spacerun: yes">&nbsp; </span>Or in your case, please start from the beginning using other genes and transcripts and tissues that interest you more than this App-Hsp84-Hars2 example.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=2>This concludes the first demonstation of how to use some of the WebQTL features. Please explore. Please also send feedback for improvements or additions to rwilliam@nb.utmem.edu</font><br> </td> </tr> </table> </body> </html> \ No newline at end of file
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- mytop = 0.49 * g_height; myleft = 0.42 * g_width; myheight = 0.07 * g_height; mywidth = 0.2 * g_width;
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- mytop = 0.5 * g_height; myleft = 0.62 * g_width; myheight = 0.36 * g_height; mywidth = 0.15 * g_width;
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- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.91 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>WebQTL can be used to enter your own trait data or to work with data that we have entered for you.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Linking to http://www.webqtl.org/search.html will get you a version of the window above. It may not be identical in layout but it will have the key features. Please follow the intructions on the slide. Of course, we encourage you to enter your own terms of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Two points: If you make a search term too complex you may get no hits. if you make it too simple (for example, APP) then you may get too much. Experiment.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>If you just link to</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>http://www.webqtl.org you will NOT see the window above but will see text that will help you to enter your own data.<span style="mso-spacerun: yes">&nbsp; </span>To get to a version of the window shown above you will need to click on the phrase<span style="mso-spacerun: yes">&nbsp; </span>RNA expression and Phenotype Databases in the upper banner.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>If you do not get a new page within 30 seconds then there is<span style="mso-spacerun: yes">&nbsp; </span>a problem: try the mirror site http://webqtl.org/search.html.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0035.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0035.htm
deleted file mode 100755
index c53c9bbc..00000000
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deleted file mode 100755
index ae7843aa..00000000
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>The Trait Data and Editing Form is the single more important page from the point of view of working with WebQTL data. Please read the text carefully. Explore the links, but do not close this page. We will need it many more times in this demonstration.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0037.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0037.htm
deleted file mode 100755
index bb776db5..00000000
--- a/web/tutorial/ppt/WebQTLDemo_files/slide0037.htm
+++ /dev/null
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0038.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0038.htm
deleted file mode 100755
index b3e613ca..00000000
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0039.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0039.htm
deleted file mode 100755
index 0b96295c..00000000
--- a/web/tutorial/ppt/WebQTLDemo_files/slide0039.htm
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>The answer is a strong Yes. A very large number of transcripts have correlations above 0.7 (absolute value) with App mRNA. The precise number today is 208. But this will change as we add more strains and arrays. In any case, this is a fairly large number and all of these correlations are significant at alpha .05 even when correcting for the enormous numbers<span style="mso-spacerun: yes">&nbsp; </span>of tests (12422 tests).</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>What does this imply?</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>That there can be massive codependence of expression variance among transcripts. App is NOT an isolated instance. This is improtant biologically and statistically. From a statistical perspective, we would like to know how many ÒindependentÓ test we effectively are performing when we use array data in this way. Are we testing 12000 independent transcripts or just 1200 transcriptional ÒmodulesÓ each with blurred boarders but each with about 10 effective members. There is no answer yet, but we probaby have a large enough data set to begin to answer this question.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0041.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0041.htm
deleted file mode 100755
index df4bc799..00000000
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Many of the data types in the previous slide are hot-linked and it is easy to generate a small web of correlations between any transcript of interest and many other transcripts. In this case, we have used green lines between transcripts that have positive correlations, and red lines between transcripts that have negative correlations. Correlations have been multiplied by 100. The correlation of 0.96 between App and Hsp84-1 reads 96.<span style="mso-spacerun: yes">&nbsp; </span>These are Pearson product moment correlations and they are sensitive to outliers. If you prefer, you can recompute Spearman rank order correlations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Where did Ndr4 (lower left) come from? It is not in the list in the previous slide. Actually it is. Nomenclature changes rapidly. If you click on R74996 in the previous slide (the active webqtl version) you will see that it now has a new symbol and name.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>What are all of the<span style="mso-spacerun: yes">&nbsp; </span>conventions in this correlation network sketch.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Times size=3>1.</font><font face=Helvetica size=3>The official gene symbol = App</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>2. OUr estimate of the location of these gene in the Mouse Genome Sequencing Consoritum version 3 build (MGSCv3). Chromosome followed by the megabase position relative to the centromere. (Mice only have one chromosome arm so this is an unambiguous coordinate. )</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>3. The pair of numbers: top is the highest expression among the strain set. The lower number is the lowest expression of that transcript among the strain set.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>4. Vertical number on the right side of each box: this is the probe set ID given by Affymetrix. We have truncated these probe set IDs so you will not see the usual<span style="mso-spacerun: yes">&nbsp; </span>ÒatÓ. A single gene may be represented by more than 10 probe sets. Thus this ID number is essential to identify the actual data source.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>5. Lower right corner: a two digit number followed by plus and minus signs. These numbers are the correlation value (absolute value) of the 100th best correlated transcript. The plus and minus signs indicate the mean polarity of the correlations.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>6. The set of numbers that read 2@140* etc. These are the approximate locations of additive effect QTLs detected by WebQTL that we will describe in other slides. Read this as: App has a suggestive QTL on Chr 2 at about 140 Mb and the D allele inherited from DBA/2J confirms a higher expression level at this marker.<span style="mso-spacerun: yes">&nbsp; </span>If there is no star symbol, then it is not even formally ÒsuggestiveÓ but does make an interesting looking blip on the QTL radar screen.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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-</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:250%; color:#E9EB5D'><i>Ndr4. A thin veneer of functional genomics.</i></span></span></layer></div> </layer></div> </LAYER>
- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Having worked with WebQTL now for 30 minutes, do we know anything new? The hypothesis that we have generated (but not validated) is that three transcripts: Atp6l, Gnas, and Ndr4 are part of a family of genes that are coregulated in normal mouse forebrain with App and Hsp84-1. We need to add functional and mechanistic significance to this hypothesis to make it biologically vibrant. But from a statiistical standpoint it is a strong inference.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Please donÕt say: But these are mere correlations. A high correlation in this context has a biological basis. The real question is are we smart enough to understand the web (not chain) of causality that produced the correlation. Once we understand the web of causality, does it have utility? Very often the answer will be NO. This will often be the case when a high correlation is generated by linkage disequilibrium of sets of polymorphisms that modulate a set of mechanistically separated traits. Chromosomal linkage can produce correlations that are not mechanistic in the conventional sense used by molecular biologists. For example, clusters<span style="mso-spacerun: yes">&nbsp; </span>of hox transcription factor genes tend to be close physically to keratin gene clusters, and one might expect shared patterns of variance produced by this linkage in a mapping panel, no matter how large.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>If Affymetrix designed probe sets with reasonable care, if we did the experiments correctly, if we sampled animals appropriately, then a correlation of 0.70 or higher between transcripts in the brain tells you that these two transcripts are effectively coupled in this set of animals under this set of conditions. More than 50% the variance in the expression of one transcript can be predicted from the other. That is a major piece of information that could be of significant clinical, economic, and predictive value, whatever its causes. Yes, correlation coefficients are noisy and have large error terms, but we have larger n of strains coming to the rescue. Expect more than 50 BXD lines soon.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>This is a thin veneer of functional genomics. It is enough to generate some marvelous hypotheses in a semi-automated way.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0046.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0046.htm
deleted file mode 100755
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0047.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0047.htm
deleted file mode 100755
index 9f73a171..00000000
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- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.18 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>C57BL/6J (B)<br> </span></div> </layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.15 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.2 * g_height; myleft = 0.3 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.3 * g_width;
-yy = 0.2 * g_height;
-ht = 0.11 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image027.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.15 * g_height; myleft = 0.62 * g_width; myheight = 0.1 * g_height; mywidth = 0.16 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.15 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.15 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>DBA/2J (D)<br> </span></div> </layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.12 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.2 * g_height; myleft = 0.66 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.66 * g_width;
-yy = 0.2 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image028.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.35 * g_height; myleft = 0.23 * g_width; myheight = 0.01 * g_height; mywidth = 0.5 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.23 * g_width;
-yy = 0.35 * g_height;
-ht = 0.01 * g_height;
-wd = 0.5 * g_width;
-document.write(
-'<img border=0 src="slide0016_image029.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.35 * g_height; myleft = 0.24 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.24 * g_width;
-yy = 0.35 * g_height;
-ht = 0.04 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image030.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.35 * g_height; myleft = 0.4 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.4 * g_width;
-yy = 0.35 * g_height;
-ht = 0.04 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image031.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.35 * g_height; myleft = 0.57 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.57 * g_width;
-yy = 0.35 * g_height;
-ht = 0.04 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image032.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.35 * g_height; myleft = 0.74 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.74 * g_width;
-yy = 0.35 * g_height;
-ht = 0.03 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image033.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.42 * g_height; myleft = 0.05 * g_width; myheight = 0.12 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F1<br> </b></span></div> </layer><script>
- mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format: lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.39 * g_height; myleft = 0.22 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.22 * g_width;
-yy = 0.39 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image034.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.39 * g_height; myleft = 0.38 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.38 * g_width;
-yy = 0.39 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image035.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.39 * g_height; myleft = 0.55 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.55 * g_width;
-yy = 0.39 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image036.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.39 * g_height; myleft = 0.72 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.72 * g_width;
-yy = 0.39 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image037.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.43 * g_height; myleft = 0.43 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.43 * g_width;
-yy = 0.43 * g_height;
-ht = 0.03 * g_height;
-wd = 0.11 * g_width;
-document.write(
-'<img border=0 src="slide0016_image038.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.6 * g_height; myleft = 0.2 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.2 * g_width;
-yy = 0.6 * g_height;
-ht = 0.03 * g_height;
-wd = 0.09 * g_width;
-document.write(
-'<img border=0 src="slide0016_image039.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.71 * g_height; myleft = 0.58 * g_width; myheight = 0.27 * g_height; mywidth = 0.2 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.03 * g_height; myleft = 0.01 * g_width; myheight = 0.22 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.15 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>20 generations </b></span></span></layer><script>
- mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>brother-sister </b></span></span></layer><script>
- mytop = 0.12 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:center'><span style='font-family:Arial;font-size:117%; color:#FFFF99'><b>matings</b></span><span style='font-family:Arial;font-size: 233%;color:#FFFF99;display:none'><b><br> </b></span></span></layer></div> </layer><script>
- mytop = 0.16 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Arial;font-size:233%; color:#FFFF99;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.6 * g_height; myleft = 0.5 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.5 * g_width;
-yy = 0.6 * g_height;
-ht = 0.03 * g_height;
-wd = 0.09 * g_width;
-document.write(
-'<img border=0 src="slide0016_image040.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.62 * g_height; myleft = 0.54 * g_width; myheight = 0.28 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.54 * g_width;
-yy = 0.62 * g_height;
-ht = 0.28 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image041.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.61 * g_height; myleft = 0.79 * g_width; myheight = 0.03 * g_height; mywidth = 0.1 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.79 * g_width;
-yy = 0.61 * g_height;
-ht = 0.03 * g_height;
-wd = 0.1 * g_width;
-document.write(
-'<img border=0 src="slide0016_image042.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.62 * g_height; myleft = 0.83 * g_width; myheight = 0.28 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.83 * g_width;
-yy = 0.62 * g_height;
-ht = 0.28 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image043.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.92 * g_height; myleft = 0.21 * g_width; myheight = 0.1 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD1</span><span style='font-family:Arial;font-size:150%;color:#484848;display:none'><br> </span></div> </layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#484848;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.92 * g_height; myleft = 0.51 * g_width; myheight = 0.1 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>BXD2<br> </span></div> </layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.92 * g_height; myleft = 0.8 * g_width; myheight = 0.06 * g_height; mywidth = 0.1 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>BXD80</span></div> </layer></div> </layer><script>
- mytop = 0.9 * g_height; myleft = 0.64 * g_width; myheight = 0.17 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.15 * g_height; mywidth = 0.16 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.16 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:300%;color:#FFFF99'>+ É +<br> </span></div> </layer><script>
- mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.13 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:300%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.62 * g_height; myleft = 0.04 * g_width; myheight = 0.12 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99'><b>F2<br> </b></span></div> </layer><script>
- mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:200%;color:#FFFF99;mso-special-format: lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.18 * g_width; myheight = 0.01 * g_height; mywidth = 0.75 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.18 * g_width;
-yy = 0.54 * g_height;
-ht = 0.01 * g_height;
-wd = 0.75 * g_width;
-document.write(
-'<img border=0 src="slide0016_image044.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.17 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.17 * g_width;
-yy = 0.54 * g_height;
-ht = 0.03 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image045.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.32 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.32 * g_width;
-yy = 0.54 * g_height;
-ht = 0.03 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image046.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.48 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.48 * g_width;
-yy = 0.54 * g_height;
-ht = 0.04 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image047.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.62 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.62 * g_width;
-yy = 0.54 * g_height;
-ht = 0.04 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image048.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.77 * g_width; myheight = 0.04 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.77 * g_width;
-yy = 0.54 * g_height;
-ht = 0.04 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image049.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.92 * g_width; myheight = 0.03 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.92 * g_width;
-yy = 0.54 * g_height;
-ht = 0.03 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image050.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.44 * g_height; myleft = 0.49 * g_width; myheight = 0.1 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.49 * g_width;
-yy = 0.44 * g_height;
-ht = 0.1 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image051.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.57 * g_height; myleft = 0.15 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.15 * g_width;
-yy = 0.57 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image052.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.57 * g_height; myleft = 0.3 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.3 * g_width;
-yy = 0.57 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image053.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.57 * g_height; myleft = 0.46 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.46 * g_width;
-yy = 0.57 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image054.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.57 * g_height; myleft = 0.6 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.6 * g_width;
-yy = 0.57 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image055.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.57 * g_height; myleft = 0.75 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.75 * g_width;
-yy = 0.57 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image056.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.57 * g_height; myleft = 0.9 * g_width; myheight = 0.11 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.9 * g_width;
-yy = 0.57 * g_height;
-ht = 0.11 * g_height;
-wd = 0.04 * g_width;
-document.write(
-'<img border=0 src="slide0016_image057.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.84 * g_height; myleft = 0.56 * g_width; myheight = 0.01 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.56 * g_width;
-yy = 0.84 * g_height;
-ht = 0.01 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image058.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.8 * g_height; myleft = 0.25 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.25 * g_width;
-yy = 0.8 * g_height;
-ht = 0.11 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image059.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.84 * g_height; myleft = 0.86 * g_width; myheight = 0.02 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.86 * g_width;
-yy = 0.84 * g_height;
-ht = 0.02 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image060.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.75 * g_height; myleft = 0.01 * g_width; myheight = 0.14 * g_height; mywidth = 0.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.13 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'><b>BXD RI<br> </b></span></div> </layer><script>
- mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.13 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'><b>Strain set<br> </b></span></div> </layer><script>
- mytop = 0.07 * g_height; myleft = 0.01 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.12 * g_height; myleft = 0.14 * g_width; myheight = 0.06 * g_height; mywidth = 0.1 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.14 * g_width;
-yy = 0.12 * g_height;
-ht = 0.06 * g_height;
-wd = 0.1 * g_width;
-document.write(
-'<img border=0 src="slide0016_image061.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.1 * g_height; myleft = 0.76 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.76 * g_width;
-yy = 0.1 * g_height;
-ht = 0.08 * g_height;
-wd = 0.14 * g_width;
-document.write(
-'<img border=0 src="slide0016_image062.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.2 * g_height; myleft = 0.17 * g_width; myheight = 0.14 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.08 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>fully<br> </span></div> </layer><script>
- mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.08 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>inbred<br> </span></div> </layer><script>
- mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.38 * g_height; myleft = 0.02 * g_width; myheight = 0.1 * g_height; mywidth = 0.12 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.07 * g_height; mywidth = 0.11 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>isogenic<br> </span></div> </layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.54 * g_height; myleft = 0.01 * g_width; myheight = 0.14 * g_height; mywidth = 0.12 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.01 * g_height; myleft = 0 * g_width; myheight = 0.11 * g_height; mywidth = 0.11 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>hetero-<br> </span></div> </layer><script>
- mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.11 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>geneous<br> </span></div> </layer><script>
- mytop = 0.07 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.74 * g_height; myleft = 0.29 * g_width; myheight = 0.22 * g_height; mywidth = 0.22 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.19 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.15 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>Recombined </span></span></layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>chromosomes </span></span></layer><script>
- mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>are needed for </span></span></layer><script>
- mytop = 0.12 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:center'><span style='font-family:Arial;font-size:150%; color:#FFFF99'>mapping<br> </span></span></layer></div> </layer><script>
- mytop = 0.16 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:150%; color:#FFFF99;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.1 * g_height; myleft = 0.25 * g_width; myheight = 0.1 * g_height; mywidth = 0.1 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>female<br> </span></div> </layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.1 * g_height; myleft = 0.64 * g_width; myheight = 0.1 * g_height; mywidth = 0.08 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99'>male<br> </span></div> </layer><script>
- mytop = 0.04 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.05 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.3 * g_height; myleft = 0.78 * g_width; myheight = 0.1 * g_height; mywidth = 0.19 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.18 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.18 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:117%;color:#FFFF99'>chromosome pair</span><span style='font-family:Arial;font-size:150%;color:#FFFF99; display:none'><br> </span></div> </layer><script>
- mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.15 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:"&#1; 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-family:Arial;font-size:150%;color:#FFFF99;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.31 * g_height; myleft = 0.65 * g_width; myheight = 0.02 * g_height; mywidth = 0.12 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.65 * g_width;
-yy = 0.31 * g_height;
-ht = 0.02 * g_height;
-wd = 0.12 * g_width;
-document.write(
-'<img border=0 src="slide0016_image063.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.86 * g_height; myleft = 0.03 * g_width; myheight = 0.14 * g_height; mywidth = 0.1 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.12 * g_height; mywidth = 0.08 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:#E9EB5D'>Inbred<br> </span></div> </layer><script>
- mytop = 0.03 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.08 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:#E9EB5D'>Isogenic<br> </span></div> </layer><script>
- mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:#E9EB5D'>siblings</span><span style='font-family:Arial;font-size:117%; color:aqua;display:none'><br> </span></div> </layer><script>
- mytop = 0.09 * g_height; myleft = 0 * g_width; myheight = 0.03 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:"&#1; 0 0";mso-margin-left-alt: 17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:117%; color:aqua;mso-special-format:lastCR;display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.79 * g_height; myleft = 0.83 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.83 * g_width;
-yy = 0.79 * g_height;
-ht = 0.11 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image064.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.79 * g_height; myleft = 0.53 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.53 * g_width;
-yy = 0.79 * g_height;
-ht = 0.11 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image065.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.8 * g_height; myleft = 0.23 * g_width; myheight = 0.11 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.23 * g_width;
-yy = 0.8 * g_height;
-ht = 0.11 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image066.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.18 * g_height; myleft = 0.43 * g_width; myheight = 0.09 * g_height; mywidth = 0.13 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.01 * g_height; myleft = 0.01 * g_width; myheight = 0.07 * g_height; mywidth = 0.12 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt:17'><span style='font-family:Arial;font-size:300%;color:aqua'>B</span><span style='font-family:Arial;font-size:300%;color:#FFFF99'>X</span><span style='font-family:Arial;font-size:300%;color:#FF1911'>D</span></div> </layer></div> </layer><script>
- mytop = 0.27 * g_height; myleft = 0.32 * g_width; myheight = 0.04 * g_height; mywidth = 0.31 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.32 * g_width;
-yy = 0.27 * g_height;
-ht = 0.04 * g_height;
-wd = 0.31 * g_width;
-document.write(
-'<img border=0 src="slide0016_image067.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.66 * g_height; myleft = 0.6 * g_width; myheight = 0.03 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.6 * g_width;
-yy = 0.66 * g_height;
-ht = 0.03 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image068.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.64 * g_height; myleft = 0.15 * g_width; myheight = 0.05 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.15 * g_width;
-yy = 0.64 * g_height;
-ht = 0.05 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image069.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.57 * g_height; myleft = 0.75 * g_width; myheight = 0.05 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.75 * g_width;
-yy = 0.57 * g_height;
-ht = 0.05 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image070.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.28 * g_height; myleft = 0.48 * g_width; myheight = 0.08 * g_height; mywidth = 0 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.48 * g_width;
-yy = 0.28 * g_height;
-ht = 0.08 * g_height;
-wd = 0 * g_width;
-document.write(
-'<img border=0 src="slide0016_image071.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.62 * g_height; myleft = 0.23 * g_width; myheight = 0.16 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.23 * g_width;
-yy = 0.62 * g_height;
-ht = 0.16 * g_height;
-wd = 0.03 * g_width;
-document.write(
-'<img border=0 src="slide0016_image072.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.61 * g_height; myleft = 0.63 * g_width; myheight = 0.08 * g_height; mywidth = 0.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.63 * g_width;
-yy = 0.61 * g_height;
-ht = 0.08 * g_height;
-wd = 0.01 * g_width;
-document.write(
-'<img border=0 src="slide0016_image073.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer></div> </LAYER>
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- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> </layer><script>
- mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
- mytop = 0.77 * g_height; myleft = 0.05 * g_width; myheight = 0.26 * g_height; mywidth = 1.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0.02 * g_height; myleft = 0.01 * g_width; myheight = 0.22 * g_height; mywidth = 1.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.14 * g_height; mywidth = 1.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:left'><span style='font-size:233%;color:#E2EBF0'>D</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%; color:#E9EB5D'>and </span><span style='font-size:233%;color:#F6BF69'>B</span><span style='font-size:233%;color:#E9EB5D'> </span><span style='font-size:200%; color:#E9EB5D'>may be SNP-like variants in the promoter </span></span></layer><script>
- mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 1 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script><span style='text-align:left'><span style='font-size:200%;color:#E9EB5D'>itself (cis QTL) or in </span><span style='font-size:200%;color:#EAEC5E'>upstream genes (trans QTLs)</span><span style='font-size:300%;color:#E9EB5D;display:none'><br> </span></span></layer></div> </layer><script>
- mytop = 0.14 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.89 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E9EB5D;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.07 * g_height; myleft = 0.04 * g_width; myheight = 0.16 * g_height; mywidth = 0.22 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.22 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D'>UPSTREAM<br> </span></div> </layer><script>
- mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.22 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D'>modulators<br> </span></div> </layer><script>
- mytop = 0.1 * g_height; myleft = 0.01 * g_width; myheight = 0.05 * g_height; mywidth = 0.2 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-size:200%;color:#E9EB5D;mso-special-format: lastCR;display:none'><br> </span></div> </layer></div> </layer><script>
- mytop = 0.07 * g_height; myleft = 0.44 * g_width; myheight = 0.18 * g_height; mywidth = 0.17 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:300%;color:#E2EBF0'>High<br> </span></div> </layer><script>
- mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:16;mso-text-indent-alt: 16'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.63 * g_height; myleft = 0.08 * g_width; myheight = 0.18 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0'>D<br> </span></div> </layer><script>
- mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#E2EBF0;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.63 * g_height; myleft = 0.15 * g_width; myheight = 0.18 * g_height; mywidth = 0.06 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.16 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.04 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69'>B<br> </span></div> </layer><script>
- mytop = 0.08 * g_height; myleft = 0 * g_width; myheight = 0.08 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:300%;color:#F6BF69;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.71 * g_height; myleft = 0.07 * g_width; myheight = 0.12 * g_height; mywidth = 0.16 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.1 * g_height; mywidth = 0.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:200%;color:#E9EB5D'><i>cis QTL</i></span><span style='font-size:200%;color:#E9EB5D;display:none'><br> </span></div> </layer><script>
- mytop = 0.05 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.12 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:200%;color:#E9EB5D;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer></div> </layer><script>
- mytop = 0.57 * g_height; myleft = -0.01 * g_width; myheight = 0.07 * g_height; mywidth = 1.01 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = -0.01 * g_width;
-yy = 0.57 * g_height;
-ht = 0.07 * g_height;
-wd = 1.01 * g_width;
-document.write(
-'<img border=0 src="slide0017_image079.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.44 * g_height; myleft = 0.47 * g_width; myheight = 0.12 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.09 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:233%;color:#F6BF69'>Low<br> </span></div> </layer><script>
- mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 0.07 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17'><span style='font-size:233%;color:#F6BF69;mso-special-format:lastCR; display:none'><br> </span></div> </layer></div> </layer><script>
- mytop = 0.58 * g_height; myleft = 0 * g_width; myheight = 0.06 * g_height; mywidth = 1.14 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script>
- mytop = 0 * g_height; myleft = 0.01 * g_width; myheight = 0.04 * g_height; mywidth = 1.13 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%;mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt: 17;mso-line-spacing:" 0 0";mso-margin-left-alt:17;mso-text-indent-alt:17'><span style='font-family:Gadget;font-size:167%;color:white'>&gt;&gt;&gt;&gt;PROMOTER--ATG-Exon1-Intron1-Exon2-Intron2 - etc-3'UTR &gt;&gt;&gt;&gt;&gt;</span></div> </layer></div> </layer><script>
- mytop = 0.37 * g_height; myleft = 0.08 * g_width; myheight = 0.2 * g_height; mywidth = 0.13 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.08 * g_width;
-yy = 0.37 * g_height;
-ht = 0.2 * g_height;
-wd = 0.13 * g_width;
-document.write(
-'<img border=0 src="slide0017_image080.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.39 * g_height; myleft = 0.1 * g_width; myheight = 0.12 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Palatino;font-size:233%; color:white'><b>&#4;<br> </b></span></div> </layer><script>
- mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Palatino;font-size:233%; color:white;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer><script>
- mytop = 0.4 * g_height; myleft = 0.17 * g_width; myheight = 0.12 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Palatino;font-size:233%; color:white'><b>&#4;<br> </b></span></div> </layer><script>
- mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Palatino;font-size:233%; color:white;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer><script>
- mytop = 0.33 * g_height; myleft = 0.08 * g_width; myheight = 0.2 * g_height; mywidth = 0.13 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div><script language=JavaScript><!--
-xx = 0.08 * g_width;
-yy = 0.33 * g_height;
-ht = 0.2 * g_height;
-wd = 0.13 * g_width;
-document.write(
-'<img border=0 src="slide0017_image081.gif" x=' + xx + ' y=' +yy + ' height=' + ht + ' width=' + wd + '>' );
-//-->
-</script></div> </layer><script>
- mytop = 0.35 * g_height; myleft = 0.17 * g_width; myheight = 0.12 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Palatino;font-size:233%; color:white'><b>&#4;<br> </b></span></div> </layer><script>
- mytop = 0.06 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:center;mso-line-spacing:" 0 0";mso-margin-left-alt:17; mso-text-indent-alt:17'><span style='font-family:Palatino;font-size:233%; color:white;mso-special-format:lastCR;display:none'><b><br> </b></span></div> </layer></div> </layer><script>
- mytop = 0.35 * g_height; myleft = 0.1 * g_width; myheight = 0.12 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
-</script> <div style='text-align:left;font-family:Verdana;font-weight:normal;font-style: normal;text-decoration:none;text-shadow:none;text-effect:none;mso-fareast-hint: no;layout-flow:horizontal;color:black;mso-color-index:1;font-size:80%; mso-text-raise:0%'><script>
- mytop = 0 * g_height; myleft = 0 * g_width; myheight = 0.05 * g_height; mywidth = 0.03 * g_width;
- document.write( '<layer top=' + mytop + ' left=' + myleft + ' height=' + myheight + ' width=' + mywidth + ' >' );
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Back to the demo. Please bring the Traiit Data and Editing window to the front and look for the Interval Mapping button. Please confirm that you are back to the trait amyloid beta precursor protein.<span style="mso-spacerun: yes">&nbsp; </span>If so, then just click the button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Notice that the default for:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Chrs (chromosomes) is ALL</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Select Probes is Probe Set</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Permuation test YES<span style="mso-spacerun: yes">&nbsp; </span>(1000 is the default number)</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Options: Bootstrap test YES (1000 is the default number)</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>This is the main output type: a so-called full genome interval map.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The X-axis represents all 19 autosomes and the X chromosome as if they were laid end to end with short gaps between the telomere of one chromosome and the centromere of the next chromosome (mouse chromsomes only have a single long arm and the centromere represents the origin of each chromosome for numerical purpose: 0 centimorgans and almost 0 megabases). The blue labels along the bottom of the figure list a subset of markers that were used in mapping. We used 753 markers to perform the mapping but here we just list five markers per chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The thick blue wavy line running across chromsomes summarizes the strength of association between variation in the phenotype (App expression differences) and the two genotypes of 753 markers and the intervals between markers (hence, interval mapping).<span style="mso-spacerun: yes">&nbsp; </span>The height of the wave (blue Y-axis to the left) provides the likelihood ratio statistic (LRS). Divide by 4.61 to convert these values to LOD scores.<span style="mso-spacerun: yes">&nbsp; </span>Or you can read them as a chi-square-like statistic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The red line and the red axis to the far right provides an estimate of the effect<span style="mso-spacerun: yes">&nbsp; </span>that a QTL has on expression of App (this estimate of the addtive effect tends to be an overestimate). If the red line is below the X-axis then this means that the allele inherited from C57BL/6J (B6 or B) at a particular marker is associated with higher values. If the red line is above the X-axis then the DBA/2J allele (D2 or D) is associated with higher traits. Multiply the additive effect size by 2 to estimate the difference between the set of strains that have the B/B genotype and the D/D genotype at a specific marker. For example, on Chr 2 the red line<span style="mso-spacerun: yes">&nbsp; </span>peaks at a value<span style="mso-spacerun: yes">&nbsp; </span>of about 0.25. That means that this region of chromosome 2 is responsible for a 0.5 unit expression difference between B/B strains and the D/D strains. Since the units are log base 2, this is 2^0.5, or about a 41% difference in expression with the D/D group being high.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The yellow histogram bars: These summarize the results of a whole-genome bootstrap of the trait that is performed 1000 times. What is a bootstrap? A bootstrap provides you a metho of evaluating whether results are robust. If we drop out one strain, do we still get the same results? When mapping quantitative traits, each strain normally gets one equally weighted vote. But inthe bootstrap procedure, we give each strain a random weighting factor of between 0 and 1.<span style="mso-spacerun: yes">&nbsp; </span>We then remap the trait and find THE SINGLE BEST LRS VALUE per bootstrap. We do this 1000 times. In this example, most bootstrap results cluster on Chr 2 under the LRS peak. That is somewhat reassuring. But notice that a substantial number of bootstrap results prefer Chr 7 or Chr 18.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The horizontal dashed lines at 9.6 and 15.9. These lines are the LRS values associated with the suggestive and significant false positive rates for genome-wide scans established by permuations of phenotypes across genotypes. We shuffle randomly 1000 times and obtain a distribution of peak LRS scores to generate a null distribution. Five percent of the time, one of these permuted data sets will have a peak LRS higher than 15.9. We call that level the 0.05 significance threshold for a whole genome scan. The p = 0.67 point is the the suggestive level, and corresponds to the green dashed line.<span style="mso-spacerun: yes">&nbsp; </span>These thresholds are conservative for transcripts that have expression variation that is highly heritable. The putative or suggestive QTL on Chr 2 is probably more than just suggestive.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>One other point: the mapping procedure we use is computationally very fast, but it is relatively simple. We are not looking for gene-gene interactions and we are not fitting multiple QTLs in combinations.<span style="mso-spacerun: yes">&nbsp; </span>Consider this QTL analysis a first pass that will highlight hot spots and warm spots that are worth following up on using more sophisticated models.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>CLICKABLE REGIONS:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>1. If you click on the Chromosome number then you will generate a new map just for that chromosome.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. If you click on the body of the map, say on the blue line, then you will generate a view<span style="mso-spacerun: yes">&nbsp; </span>on a 10 Mb window of that part of the genome from the UCSC Genome Browser web site.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>3. If you click on a marker symbol, then you will generate a new Trait data and editing window with the genotypes loaded into the window just like any other trait. More on this later.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>NOTE: you can drag these maps off of the browser window and onto your desktop. The will be saved as PNG or PDF files. You can import them into Photoshop or other programs.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>App has a suggestive QTL on Chr 2. What about the neighbors that we defined as having shared expression patterns. This figure shows that members of the immediate App neigborhood share a weak Chr 2 QTL.<span style="mso-spacerun: yes">&nbsp; </span>That is what the blue oval in the background is meant to represent. But some transcripts, such as Ndr4 and Psen2 do not share this Chr 2 interval.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>QUESTION: What kind of headway can we make in detemining what polymorphism or polymorphisms on Chr 2 near 130 Mb might contribute to the variance in the expression of all of these important transcripts?</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4><b>Candidate Genes: </b><span style="mso-spacerun: yes">&nbsp;</span>The best we can do at this point is to make an educated guess about the candidacy status of all genes in the QTL support interval. For sake of argument, lets say that we are confident that the polymorphism is located between 130 and 150 Mb (20 Mb, equivalent to roughly 10 cM). There will typically be 12 to 15 genes per Mb, so we now would like to evaluate 240 to 300 positional candidates. We would like to highlight the biologically relevant subset of candidates. We could look through gene ontologies and expression levels to help us winnow the list. An alternate way avaiable using WebQTL is to generate a list of those genes in this 20 Mb interval that have transcripts that co-vary in expression with App expression.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>To do this, go back to the Trait Data and Editing window. Sort the correlations by position. Select Return = 500. Then scroll down the list to see positional candidates that share expression with App.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face="Times New Roman" size=4>There are several candidates that have high correlation with App even in this short 20 Mb interval. We can rank them by correlation, but they are all close.<span style="mso-spacerun: yes">&nbsp; </span>There is one other imporant approach to ranking these candidates. Are they likely to contain polymorphisms? We can assess the likelihood that they contain polymorphisms by mapping each transcript to see if any have strong cis QTLs. The logic of this search is that a transcript that has a strong cis-QTL is likely to contain functional polymorphisms that effect its own expression. This make is more like that the transcript is a ÒcausativeÓ factor since it is likely to be polymorphic.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0054.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0054.htm
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0055.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0055.htm
deleted file mode 100755
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>LetÕs look at Hars2 in more detail by mapping all of the perfect match probes (16 of them) that target this transcript.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Go back to the Trait Data and Editing window and select Chr 2 (rather than ALL as shown above) and also select PM Probes. Then click on Interval Mapping button.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>You will get the illustration above, but without the sequence data that we have added.<span style="mso-spacerun: yes">&nbsp; </span>The 16 perfect match probes are arranged in sequence (red is 5 prime, blue is the 3 prime end). For example, the 5 prime-most primer 307387 has the sequence CACTG..... It also has a polymorphism at the 17 nucleotide of this 25 nt probe sequence.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>How do we know that the 5 prime probe is polymorphic? By looking up the sequence in the Celera Genomics databases which often contqains sequence data for C57BL/6J (B6 above) and for DBA/2J.<span style="mso-spacerun: yes">&nbsp; </span>But two blue probes (14 and 15) do NOT contain SNPs but still have very large LRS scores. The other probes do not perform so wel. Highly variable probe performance is probably a result of the very different stacking energies of DNA-RNA duplexes.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>Part 3.<span style="mso-spacerun: yes">&nbsp; </span>Many investigators would like to discover the set of downstream targets of a gene of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Helvetica size=3>In a genetic and functional sense, that question can only be addressed effectively if there is genetic variation in the particular gene.<span style="mso-spacerun: yes">&nbsp; </span>We know that Fos is an important transcription factor, but unless it is polymorphic between C57BL/6J and DBA/2J, then it cannot generate a genetic variance signal with which we can work. We can still study covariance of Fos and hundreds of other transcripts (an interesting exercise), but there are no genetic causes-and-effects.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0059.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0059.htm
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-</script><span style='text-align:left'><span style='font-family:"Gill Sans";font-size:267%; color:#E9EB5D'><i>other traits.</i></span></span></layer></div> </layer></div> </LAYER>
- <layer> <div id=NotesObj style='display:none'> <table style='color:white' border=0 width="100%"> <tr> <td width=5 nowrap></td> <td width="100%"></td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Genes must be polymorphic to generate downstream genetic effects (as opposed to downstream molecular effects). Hars2 meets this condition because we have already mapped a functional polymorphism in the gene. We can therefore posit that Hars2 is a QT gene. What transcripts are downstream? <i>App</i> is one obvious candidate, but there are many more.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>The are several ways to look for downstream targets. The best and most obvious is to look up all transcripts that have high correlations with Hars2 itself. You should know how to do this. An alternative method is shown here for teaching purpose and to show you what to do if your gene of interest is not in our database. You need to know:</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Times size=3>1.</font><font face=Verdana size=3> Where your gene is located. You need this information to find a surrogate marker; a marker that is located very close to your gene of interest.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>2. That your gene is polymorphic between C57BL/6J and DBA/2J.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>LetÕs look at the correlation of Hars2 with BXD genotypes as shown in the slide above to illustrate how to use markers as surrogate traits.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>Go to the Trait Date and Editing window one more time. We want the data for Hars2 this time, not App. You should be able to show that Hars2 has a high<span style="mso-spacerun: yes">&nbsp; </span>correlation with D2Mit423 as shown in the slide above.</font><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><br> </td> </tr> <tr> <td colspan=1></td> <td align=left colspan=1><font face=Verdana size=3>By clicking on the symbol D2Mit423 in the Correlation window, you will generate a new Trait Data window shown on the next slide.</font><br> </td> </tr> </table> </div> </layer> <script language=JavaScript><!--
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0060.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0060.htm
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0061.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0061.htm
deleted file mode 100755
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0062.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0062.htm
deleted file mode 100755
index c8c24f70..00000000
--- a/web/tutorial/ppt/WebQTLDemo_files/slide0062.htm
+++ /dev/null
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diff --git a/web/tutorial/ppt/WebQTLDemo_files/slide0064.htm b/web/tutorial/ppt/WebQTLDemo_files/slide0064.htm
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