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authoracenteno2020-04-21 17:35:34 -0500
committerGitHub2020-04-21 17:35:34 -0500
commit660589b9c2a507529e8e51ca6ce66ca97ad982c5 (patch)
tree27f63957278581bc2fce2b88744bfe20c8a81558 /wqflask/utility/gen_geno_ob.py
parentd97fdc18359233f07c1a1c7b83fe7e88eb225043 (diff)
parentf2a3ae13231a7d270a5bb6911c248aa713f1ef91 (diff)
downloadgenenetwork2-660589b9c2a507529e8e51ca6ce66ca97ad982c5.tar.gz
Merge pull request #1 from genenetwork/testing
Updating my testing branch
Diffstat (limited to 'wqflask/utility/gen_geno_ob.py')
-rw-r--r--wqflask/utility/gen_geno_ob.py181
1 files changed, 181 insertions, 0 deletions
diff --git a/wqflask/utility/gen_geno_ob.py b/wqflask/utility/gen_geno_ob.py
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+from __future__ import absolute_import, division, print_function
+
+import utility.logger
+logger = utility.logger.getLogger(__name__ )
+
+class genotype(object):
+ """
+ Replacement for reaper.Dataset so we can remove qtlreaper use while still generating mapping output figure
+ """
+
+ def __init__(self, filename):
+ self.group = None
+ self.type = "riset"
+ self.prgy = []
+ self.nprgy = 0
+ self.mat = -1
+ self.pat = 1
+ self.het = 0
+ self.unk = "U"
+ self.filler = False
+ self.mb_exists = False
+
+ #ZS: This is because I'm not sure if some files switch the column that contains Mb/cM positions; might be unnecessary
+ self.cm_column = 2
+ self.mb_column = 3
+
+ self.chromosomes = []
+
+ self.read_file(filename)
+
+ def __iter__(self):
+ return iter(self.chromosomes)
+
+ def __getitem__(self, index):
+ return self.chromosomes[index]
+
+ def __len__(self):
+ return len(self.chromosomes)
+
+ def read_rdata_output(self, qtl_results):
+ #ZS: This is necessary because R/qtl requires centimorgan marker positions, which it normally gets from the .geno file, but that doesn't exist for HET3-ITP (which only has RData), so it needs to read in the marker cM positions from the results
+ self.chromosomes = [] #ZS: Overwriting since the .geno file's contents are just placeholders
+
+ this_chr = "" #ZS: This is so it can track when the chromosome changes as it iterates through markers
+ chr_ob = None
+ for marker in qtl_results:
+ locus = Locus(self)
+ if (str(marker['chr']) != this_chr) and this_chr != "X": #ZS: This is really awkward but works as a temporary fix
+ if this_chr != "":
+ self.chromosomes.append(chr_ob)
+ this_chr = str(marker['chr'])
+ if this_chr == "20":
+ this_chr = "X"
+ chr_ob = Chr(this_chr, self)
+ if 'chr' in marker:
+ locus.chr = str(marker['chr'])
+ if 'name' in marker:
+ locus.name = marker['name']
+ if 'Mb' in marker:
+ locus.Mb = marker['Mb']
+ if 'cM' in marker:
+ locus.cM = marker['cM']
+ chr_ob.loci.append(locus)
+
+ self.chromosomes.append(chr_ob)
+
+ return self
+
+ def read_file(self, filename):
+ with open(filename, 'r') as geno_file:
+ lines = geno_file.readlines()
+
+ this_chr = "" #ZS: This is so it can track when the chromosome changes as it iterates through markers
+ chr_ob = None
+ for line in lines:
+ if line[0] == "#":
+ continue
+ elif line[0] == "@":
+ label = line.split(":")[0][1:]
+ if label == "name":
+ self.group = line.split(":")[1].strip()
+ elif label == "filler":
+ if line.split(":")[1].strip() == "yes":
+ self.filler = True
+ elif label == "type":
+ self.type = line.split(":")[1].strip()
+ elif label == "mat":
+ self.mat = line.split(":")[1].strip()
+ elif label == "pat":
+ self.pat = line.split(":")[1].strip()
+ elif label == "het":
+ self.het = line.split(":")[1].strip()
+ elif label == "unk":
+ self.unk = line.split(":")[1].strip()
+ else:
+ continue
+ elif line[:3] == "Chr":
+ header_row = line.split("\t")
+ if header_row[2] == "Mb":
+ self.mb_exists = True
+ self.mb_column = 2
+ self.cm_column = 3
+ elif header_row[3] == "Mb":
+ self.mb_exists = True
+ self.mb_column = 3
+ elif header_row[2] == "cM":
+ self.cm_column = 2
+
+ if self.mb_exists:
+ self.prgy = header_row[4:]
+ else:
+ self.prgy = header_row[3:]
+ self.nprgy = len(self.prgy)
+ else:
+ if line.split("\t")[0] != this_chr:
+ if this_chr != "":
+ self.chromosomes.append(chr_ob)
+ this_chr = line.split("\t")[0]
+ chr_ob = Chr(line.split("\t")[0], self)
+ chr_ob.add_marker(line.split("\t"))
+
+ self.chromosomes.append(chr_ob)
+
+class Chr(object):
+ def __init__(self, name, geno_ob):
+ self.name = name
+ self.loci = []
+ self.mb_exists = geno_ob.mb_exists
+ self.cm_column = geno_ob.cm_column
+ self.mb_column = geno_ob.mb_column
+ self.geno_ob = geno_ob
+
+ def __iter__(self):
+ return iter(self.loci)
+
+ def __getitem__(self, index):
+ return self.loci[index]
+
+ def __len__(self):
+ return len(self.loci)
+
+ def add_marker(self, marker_row):
+ self.loci.append(Locus(self.geno_ob, marker_row))
+
+class Locus(object):
+ def __init__(self, geno_ob, marker_row = None):
+ self.chr = None
+ self.name = None
+ self.cM = None
+ self.Mb = None
+ self.genotype = []
+ if marker_row:
+ self.chr = marker_row[0]
+ self.name = marker_row[1]
+ try:
+ self.cM = float(marker_row[geno_ob.cm_column])
+ except:
+ self.cM = float(marker_row[geno_ob.mb_column]) if geno_ob.mb_exists else 0
+ try:
+ self.Mb = float(marker_row[geno_ob.mb_column]) if geno_ob.mb_exists else None
+ except:
+ self.Mb = self.cM
+
+ geno_table = {
+ geno_ob.mat: -1,
+ geno_ob.pat: 1,
+ geno_ob.het: 0,
+ geno_ob.unk: "U"
+ }
+
+ self.genotype = []
+ if geno_ob.mb_exists:
+ start_pos = 4
+ else:
+ start_pos = 3
+
+ for allele in marker_row[start_pos:]:
+ if allele in geno_table.keys():
+ self.genotype.append(geno_table[allele])
+ else: #ZS: Some genotype appears that isn't specified in the metadata, make it unknown
+ self.genotype.append("U") \ No newline at end of file