The goal of this project is to study expression of a large set of defined and undefined metabolites in fecal samples from a genetically diverse set of BXD mouse strains (females) raised either on a high fat diet or low fat diet. Samples were taken from animals at sacrifices directly from the large intestine.

Aim: Effect of genetics and diet on fecal metabolomics

Use UPLC-MS Broad Spectrum Metabolomics to study:

To capture the most signal, all samples were analyzed under positive and negative ion mode using a reversed-phase separation.