Sample Preparation Summary

• Feces were extracted in 95:5 water:methanol
• Extracts were prepared for UPLC-QTOF-MS analysis

Instruments for LC-MS Analysis
Waters SYNAPT G2 QTOF with Acquity UPLC (Waters Corporation, MA)
MS data collected over 50-1000 m/z was collected in the positive and negative ion modes.

UPLC-MS Solutions(Reversed Phase)
Mobile Phase A (Reversed-Phase)—Water with 0.1% Formic Acid
Mobile Phase B (Reversed-Phase)—Methanol with 0.1% Formic Acid
Column: Waters Acquity BEH HSS T3, 2.1 x 100 mm at 50 °C