The study included 241 females (aged 50 to 90 ± 2 days at the start of the experiment) from B6, D2, and 35 advanced intercross BXD strains (about 2 mice per strain and per condition). Mice were divided into 4 groups of Control (CTL, 53 mice for 31 strains), Chronic Mild Stress (CMS, 64 mice for 32 strains), Drinking in Dark (DID, 64 mice for 35 strains), and CMS+DID (60 mice for 35 strains). Mice in CTL were normally handled, while mice in other groups were singly housed in shoebox cages, with food and water provided ad libitum except when the water was replaced by 20% (v/v) EtOH (see below) for DID and CMS+DID groups (detailed treatments and schemes are showing in the Table and Figure). The ambient temperature was 21 ± 2°C, and the light cycle was 7:00/19:00 for CTL, and 23:00/11:00 the rest groups lights on/off. The animals were weighed weekly to assess health and to provide the basis for calculating the amount of EtOH consumed in grams per kilogram. All procedures were approved by the UTHSC Institutional Animal Care and Use Committee.
The table of samples that are finally used for this study.
|
Index |
ID |
Strain |
Sex |
Treatment Group |
Tissue |
|
1 |
CMS030716_12 |
BXD68 |
F |
CMS |
hippocampus |
|
2 |
CMS030716_18 |
BXD40 |
F |
CMS |
hippocampus |
|
3 |
CMS030716_19 |
BXD40 |
F |
CMS |
hippocampus |
|
4 |
CMS030916_02 |
BXD101 |
F |
CMS |
hippocampus |
|
5 |
CMS030916_09 |
BXD101 |
F |
CMS |
hippocampus |
|
6 |
CMS030916_11 |
BXD79 |
F |
CMS |
hippocampus |
|
7 |
CMS030916_14 |
BXD48a |
F |
CMS |
hippocampus |
|
8 |
CMS030916_20 |
BXD65b |
F |
CMS |
hippocampus |
|
9 |
CMS030916_23 |
BXD65b |
F |
CMS |
hippocampus |
|
10 |
CMS030916_32 |
BXD86 |
F |
CMS |
hippocampus |
|
11 |
CMS032817_02 |
BXD48 |
F |
CMS |
hippocampus |
|
12 |
CMS032817_28 |
BXD66 |
F |
CMS |
hippocampus |
|
13 |
CMS032917_08 |
BXD48 |
F |
CMS |
hippocampus |
|
14 |
CMS032917_24 |
BXD70 |
F |
CMS |
hippocampus |
|
15 |
CMS032917_26 |
BXD87 |
F |
CMS |
hippocampus |
|
16 |
CMS032917_33 |
BXD70 |
F |
CMS |
hippocampus |
|
17 |
CMS033017_05 |
BXD78 |
F |
CMS |
hippocampus |
|
18 |
CMS033017_25 |
BXD78 |
F |
CMS |
hippocampus |
|
19 |
CMS061218_12 |
BXD87 |
F |
CMS |
hippocampus |
|
20 |
CMS061218_13 |
BXD60 |
F |
CMS |
hippocampus |
|
21 |
CMS061218_14 |
BXD71 |
F |
CMS |
hippocampus |
|
22 |
CMS071216_17 |
BXD24 |
F |
CMS |
hippocampus |
|
23 |
CMS071216_23 |
BXD73B |
F |
CMS |
hippocampus |
|
24 |
CMS071216_25 |
BXD48a |
F |
CMS |
hippocampus |
|
25 |
CMS071316_06 |
BXD90 |
F |
CMS |
hippocampus |
|
26 |
CMS071316_08 |
BXD66 |
F |
CMS |
hippocampus |
|
27 |
CMS071316_14 |
BXD87 |
F |
CMS |
hippocampus |
|
28 |
CMS071316_17 |
BXD62 |
F |
CMS |
hippocampus |
|
29 |
CMS071316_18 |
BXD65 |
F |
CMS |
hippocampus |
|
30 |
CMS071316_32 |
BXD90 |
F |
CMS |
hippocampus |
|
31 |
CMS071416_06 |
BXD50 |
F |
CMS |
hippocampus |
|
32 |
CMS082817_07 |
BXD44 |
F |
CMS |
hippocampus |
|
33 |
CMS082817_25 |
BXD71 |
F |
CMS |
hippocampus |
|
34 |
CMS082917_16 |
BXD79 |
F |
CMS |
hippocampus |
|
35 |
CMS083017_07 |
BXD69 |
F |
CMS |
hippocampus |
|
36 |
CMS083017_25 |
BXD32 |
F |
CMS |
hippocampus |
|
37 |
CMS092018_01 |
DBA/2J |
F |
CMS |
hippocampus |
|
38 |
CMS092018_05 |
C57BL/6J |
F |
CMS |
hippocampus |
|
39 |
CMS092118_08 |
C57BL/6J |
F |
CMS |
hippocampus |
|
40 |
CMS102716_01 |
BXD83 |
F |
CMS |
hippocampus |
|
41 |
CMS102716_02 |
BXD75 |
F |
CMS |
hippocampus |
|
42 |
CMS102716_03 |
BXD73a |
F |
CMS |
hippocampus |
|
43 |
CMS102716_05 |
BXD73a |
F |
CMS |
hippocampus |
|
44 |
CMS102716_06 |
BXD34 |
F |
CMS |
hippocampus |
|
45 |
CMS102716_07 |
BXD24 |
F |
CMS |
hippocampus |
|
46 |
CMS102716_08 |
BXD60 |
F |
CMS |
hippocampus |
|
47 |
CMS102716_11 |
BXD34 |
F |
CMS |
hippocampus |
|
48 |
CMS102716_12 |
BXD62 |
F |
CMS |
hippocampus |
|
49 |
CMS102716_14 |
BXD83 |
F |
CMS |
hippocampus |
|
50 |
CMS102716_15 |
BXD69 |
F |
CMS |
hippocampus |
|
51 |
CMS102716_16 |
BXD77 |
F |
CMS |
hippocampus |
|
52 |
CMS102716_20 |
BXD50 |
F |
CMS |
hippocampus |
|
53 |
CMS102816_02 |
C57BL/6J |
F |
CMS |
hippocampus |
|
54 |
CMS102816_03 |
C57BL/6J |
F |
CMS |
hippocampus |
|
55 |
CMS102816_04 |
DBA/2J |
F |
CMS |
hippocampus |
|
56 |
CMS102816_06 |
DBA/2J |
F |
CMS |
hippocampus |
|
57 |
CMS102816_10 |
C57BL/6J |
F |
CMS |
hippocampus |
|
58 |
CMS102816_11 |
BXD100 |
F |
CMS |
hippocampus |
|
59 |
CMS102816_13 |
BXD75 |
F |
CMS |
hippocampus |
|
60 |
CMS102816_14 |
BXD77 |
F |
CMS |
hippocampus |
|
61 |
CMS102816_17 |
BXD100 |
F |
CMS |
hippocampus |
|
62 |
CMS102816_18 |
BXD43 |
F |
CMS |
hippocampus |
|
63 |
CMS102816_20 |
BXD32 |
F |
CMS |
hippocampus |
|
64 |
CMS102816_21 |
BXD65 |
F |
CMS |
hippocampus |
|
65 |
CMS030716_01 |
BXD55 |
F |
CMS+DID |
hippocampus |
|
66 |
CMS030716_08 |
BXD63 |
F |
CMS+DID |
hippocampus |
|
67 |
CMS030716_09 |
BXD65b |
F |
CMS+DID |
hippocampus |
|
68 |
CMS030716_15 |
BXD69 |
F |
CMS+DID |
hippocampus |
|
69 |
CMS030716_27 |
BXD86 |
F |
CMS+DID |
hippocampus |
|
70 |
CMS030716_29 |
BXD55 |
F |
CMS+DID |
hippocampus |
|
71 |
CMS030716_30 |
BXD62 |
F |
CMS+DID |
hippocampus |
|
72 |
CMS030716_31 |
BXD65b |
F |
CMS+DID |
hippocampus |
|
73 |
CMS030916_15 |
BXD69 |
F |
CMS+DID |
hippocampus |
|
74 |
CMS030916_16 |
BXD68 |
F |
CMS+DID |
hippocampus |
|
75 |
CMS030916_17 |
BXD40 |
F |
CMS+DID |
hippocampus |
|
76 |
CMS030916_26 |
BXD79 |
F |
CMS+DID |
hippocampus |
|
77 |
CMS032817_10 |
BXD87 |
F |
CMS+DID |
hippocampus |
|
78 |
CMS032817_18 |
BXD70 |
F |
CMS+DID |
hippocampus |
|
79 |
CMS032817_25 |
BXD48 |
F |
CMS+DID |
hippocampus |
|
80 |
CMS032817_27 |
BXD83 |
F |
CMS+DID |
hippocampus |
|
81 |
CMS032817_31 |
BXD71 |
F |
CMS+DID |
hippocampus |
|
82 |
CMS032817_32 |
BXD70 |
F |
CMS+DID |
hippocampus |
|
83 |
CMS032917_15 |
BXD86 |
F |
CMS+DID |
hippocampus |
|
84 |
CMS032917_17 |
BXD71 |
F |
CMS+DID |
hippocampus |
|
85 |
CMS032917_25 |
BXD78 |
F |
CMS+DID |
hippocampus |
|
86 |
CMS033017_04 |
BXD78 |
F |
CMS+DID |
hippocampus |
|
87 |
CMS033017_11 |
BXD83 |
F |
CMS+DID |
hippocampus |
|
88 |
CMS033017_13 |
BXD73 |
F |
CMS+DID |
hippocampus |
|
89 |
CMS071216_01 |
BXD50 |
F |
CMS+DID |
hippocampus |
|
90 |
CMS071216_24 |
BXD100 |
F |
CMS+DID |
hippocampus |
|
91 |
CMS071316_05 |
BXD51 |
F |
CMS+DID |
hippocampus |
|
92 |
CMS071316_20 |
BXD65 |
F |
CMS+DID |
hippocampus |
|
93 |
CMS071316_22 |
BXD90 |
F |
CMS+DID |
hippocampus |
|
94 |
CMS071316_29 |
BXD51 |
F |
CMS+DID |
hippocampus |
|
95 |
CMS071416_01 |
BXD100 |
F |
CMS+DID |
hippocampus |
|
96 |
CMS071416_08 |
BXD24 |
F |
CMS+DID |
hippocampus |
|
97 |
CMS071416_13 |
BXD48a |
F |
CMS+DID |
hippocampus |
|
98 |
CMS071416_19 |
BXD32 |
F |
CMS+DID |
hippocampus |
|
99 |
CMS071416_27 |
BXD63 |
F |
CMS+DID |
hippocampus |
|
100 |
CMS082817_02 |
BXD48 |
F |
CMS+DID |
hippocampus |
|
101 |
CMS082817_03 |
BXD40 |
F |
CMS+DID |
hippocampus |
|
102 |
CMS082817_09 |
DBA/2J |
F |
CMS+DID |
hippocampus |
|
103 |
CMS082817_28 |
BXD79 |
F |
CMS+DID |
hippocampus |
|
104 |
CMS082917_07 |
BXD43 |
F |
CMS+DID |
hippocampus |
|
105 |
CMS082917_10 |
BXD44 |
F |
CMS+DID |
hippocampus |
|
106 |
CMS082917_18 |
BXD32 |
F |
CMS+DID |
hippocampus |
|
107 |
CMS082917_21 |
BXD24 |
F |
CMS+DID |
hippocampus |
|
108 |
CMS082917_29 |
BXD87 |
F |
CMS+DID |
hippocampus |
|
109 |
CMS083017_02 |
BXD101 |
F |
CMS+DID |
hippocampus |
|
110 |
CMS083017_03 |
BXD66 |
F |
CMS+DID |
hippocampus |
|
111 |
CMS083017_11 |
DBA/2J |
F |
CMS+DID |
hippocampus |
|
112 |
CMS083017_28 |
BXD101 |
F |
CMS+DID |
hippocampus |
|
113 |
CMS111716_07 |
BXD75 |
F |
CMS+DID |
hippocampus |
|
114 |
CMS111716_11 |
BXD73b |
F |
CMS+DID |
hippocampus |
|
115 |
CMS111716_12 |
BXD75 |
F |
CMS+DID |
hippocampus |
|
116 |
CMS111716_14 |
BXD73a |
F |
CMS+DID |
hippocampus |
|
117 |
CMS111716_17 |
BXD50 |
F |
CMS+DID |
hippocampus |
|
118 |
CMS111816_05 |
BXD77 |
F |
CMS+DID |
hippocampus |
|
119 |
CMS111816_07 |
BXD73b |
F |
CMS+DID |
hippocampus |
|
120 |
CMS111816_12 |
BXD73a |
F |
CMS+DID |
hippocampus |
|
121 |
CMS111816_13 |
BXD77 |
F |
CMS+DID |
hippocampus |
|
122 |
CMS111816_26 |
BXD34 |
F |
CMS+DID |
hippocampus |
|
123 |
S32315_14 |
C57BL/6J |
F |
CMS+DID |
hippocampus |
|
124 |
S32415_09 |
BXD68 |
F |
CMS+DID |
hippocampus |
|
125 |
CMS030716_05 |
BXD44 |
F |
DID |
hippocampus |
|
126 |
CMS030716_14 |
BXD40 |
F |
DID |
hippocampus |
|
127 |
CMS030716_21 |
BXD68 |
F |
DID |
hippocampus |
|
128 |
CMS030716_24 |
BXD48a |
F |
DID |
hippocampus |
|
129 |
CMS030716_28 |
BXD79 |
F |
DID |
hippocampus |
|
130 |
CMS030716_33 |
BXD65b |
F |
DID |
hippocampus |
|
131 |
CMS030916_04 |
BXD55 |
F |
DID |
hippocampus |
|
132 |
CMS030916_06 |
BXD65b |
F |
DID |
hippocampus |
|
133 |
CMS030916_07 |
BXD60 |
F |
DID |
hippocampus |
|
134 |
CMS030916_10 |
BXD63 |
F |
DID |
hippocampus |
|
135 |
CMS030916_18 |
BXD65b |
F |
DID |
hippocampus |
|
136 |
CMS030916_22 |
BXD79 |
F |
DID |
hippocampus |
|
137 |
CMS030916_24 |
BXD63 |
F |
DID |
hippocampus |
|
138 |
CMS030916_29 |
BXD55 |
F |
DID |
hippocampus |
|
139 |
CMS030916_33 |
BXD69 |
F |
DID |
hippocampus |
|
140 |
CMS032817_08 |
BXD73 |
F |
DID |
hippocampus |
|
141 |
CMS032817_24 |
BXD70 |
F |
DID |
hippocampus |
|
142 |
CMS032917_07 |
BXD70 |
F |
DID |
hippocampus |
|
143 |
CMS032917_19 |
BXD78 |
F |
DID |
hippocampus |
|
144 |
CMS032917_21 |
BXD86 |
F |
DID |
hippocampus |
|
145 |
CMS032917_23 |
BXD66 |
F |
DID |
hippocampus |
|
146 |
CMS032917_30 |
BXD78 |
F |
DID |
hippocampus |
|
147 |
CMS032917_31 |
BXD48 |
F |
DID |
hippocampus |
|
148 |
CMS032917_32 |
BXD71 |
F |
DID |
hippocampus |
|
149 |
CMS033017_16 |
BXD62 |
F |
DID |
hippocampus |
|
150 |
CMS033017_29 |
BXD83 |
F |
DID |
hippocampus |
|
151 |
CMS071216_04 |
BXD90 |
F |
DID |
hippocampus |
|
152 |
CMS071216_09 |
BXD77 |
F |
DID |
hippocampus |
|
153 |
CMS071216_16 |
BXD65 |
F |
DID |
hippocampus |
|
154 |
CMS071216_22 |
BXD48a |
F |
DID |
hippocampus |
|
155 |
CMS071316_15 |
BXD90 |
F |
DID |
hippocampus |
|
156 |
CMS071316_16 |
BXD50 |
F |
DID |
hippocampus |
|
157 |
CMS071316_27 |
BXD65 |
F |
DID |
hippocampus |
|
158 |
CMS071316_30 |
BXD51 |
F |
DID |
hippocampus |
|
159 |
CMS071416_10 |
BXD44 |
F |
DID |
hippocampus |
|
160 |
CMS071416_21 |
BXD69 |
F |
DID |
hippocampus |
|
161 |
CMS082817_12 |
BXD51 |
F |
DID |
hippocampus |
|
162 |
CMS082817_16 |
BXD101 |
F |
DID |
hippocampus |
|
163 |
CMS082817_22 |
BXD101 |
F |
DID |
hippocampus |
|
164 |
CMS082817_23 |
BXD24 |
F |
DID |
hippocampus |
|
165 |
CMS082917_13 |
BXD40 |
F |
DID |
hippocampus |
|
166 |
CMS082917_22 |
BXD87 |
F |
DID |
hippocampus |
|
167 |
CMS083017_10 |
BXD24 |
F |
DID |
hippocampus |
|
168 |
CMS102215_20 |
BXD73B |
F |
DID |
hippocampus |
|
169 |
CMS111716_02 |
BXD75 |
F |
DID |
hippocampus |
|
170 |
CMS111716_05 |
BXD43 |
F |
DID |
hippocampus |
|
171 |
CMS111716_08 |
BXD75 |
F |
DID |
hippocampus |
|
172 |
CMS111716_10 |
BXD32 |
F |
DID |
hippocampus |
|
173 |
CMS111716_16 |
BXD100 |
F |
DID |
hippocampus |
|
174 |
CMS111716_20 |
BXD60 |
F |
DID |
hippocampus |
|
175 |
CMS111716_22 |
BXD83 |
F |
DID |
hippocampus |
|
176 |
CMS111716_23 |
BXD73b |
F |
DID |
hippocampus |
|
177 |
CMS111716_25 |
BXD73a |
F |
DID |
hippocampus |
|
178 |
CMS111816_01 |
BXD34 |
F |
DID |
hippocampus |
|
179 |
CMS111816_03 |
BXD48 |
F |
DID |
hippocampus |
|
180 |
CMS111816_08 |
BXD43 |
F |
DID |
hippocampus |
|
181 |
CMS111816_11 |
BXD87 |
F |
DID |
hippocampus |
|
182 |
CMS111816_14 |
BXD60 |
F |
DID |
hippocampus |
|
183 |
CMS111816_16 |
BXD73b |
F |
DID |
hippocampus |
|
184 |
CMS111816_17 |
BXD77 |
F |
DID |
hippocampus |
|
185 |
CMS111816_20 |
BXD73a |
F |
DID |
hippocampus |
|
186 |
CMS111816_28 |
BXD50 |
F |
DID |
hippocampus |
|
187 |
S32515_12 |
BXD68 |
F |
DID |
hippocampus |
|
188 |
S32515_16 |
C57BL/6J |
F |
DID |
hippocampus |
|
189 |
13118_35 |
BXD62 |
F |
CTL |
hippocampus |
|
190 |
13118_41 |
BXD66 |
F |
CTL |
hippocampus |
|
191 |
13118_56 |
BXD75 |
F |
CTL |
hippocampus |
|
192 |
31218_19 |
BXD65b |
F |
CTL |
hippocampus |
|
193 |
31218_3 |
BXD73a |
F |
CTL |
hippocampus |
|
194 |
31218_36 |
BXD78 |
F |
CTL |
hippocampus |
|
195 |
AGE041118_03 |
C57BL/6J |
F |
CTL |
hippocampus |
|
196 |
AGE050118_07 |
BXD55 |
F |
CTL |
hippocampus |
|
197 |
AGE050118_12 |
BXD73 |
F |
CTL |
hippocampus |
|
198 |
AGE050118_16 |
BXD77 |
F |
CTL |
hippocampus |
|
199 |
AGE050118_21 |
BXD86 |
F |
CTL |
hippocampus |
|
200 |
AGE050118_23 |
BXD86 |
F |
CTL |
hippocampus |
|
201 |
AGE050118_27 |
DBA/2J |
F |
CTL |
hippocampus |
|
202 |
AGE061818_02 |
BXD100 |
F |
CTL |
hippocampus |
|
203 |
BL013118_12 |
BXD34 |
F |
CTL |
hippocampus |
|
204 |
BL013118_13 |
BXD34 |
F |
CTL |
hippocampus |
|
205 |
CMS092118_24 |
C57BL/6J |
F |
CTL |
hippocampus |
|
206 |
E100118_16 |
BXD65 |
F |
CTL |
hippocampus |
|
207 |
MT052417_09 |
BXD40 |
F |
CTL |
hippocampus |
|
208 |
MT052417_17 |
BXD51 |
F |
CTL |
hippocampus |
|
209 |
S13118_07 |
BXD24 |
F |
CTL |
hippocampus |
|
210 |
S13118_08 |
BXD24 |
F |
CTL |
hippocampus |
|
211 |
S13118_15 |
BXD40 |
F |
CTL |
hippocampus |
|
212 |
S13118_18 |
BXD43 |
F |
CTL |
hippocampus |
|
213 |
S13118_19 |
BXD43 |
F |
CTL |
hippocampus |
|
214 |
S13118_23 |
BXD48 |
F |
CTL |
hippocampus |
|
215 |
S13118_24 |
BXD48 |
F |
CTL |
hippocampus |
|
216 |
S13118_26 |
BXD48a |
F |
CTL |
hippocampus |
|
217 |
S13118_36 |
BXD62 |
F |
CTL |
hippocampus |
|
218 |
S13118_38 |
BXD65 |
F |
CTL |
hippocampus |
|
219 |
S13118_42 |
BXD66 |
F |
CTL |
hippocampus |
|
220 |
S13118_44 |
BXD68 |
F |
CTL |
hippocampus |
|
221 |
S13118_58 |
BXD79 |
F |
CTL |
hippocampus |
|
222 |
S13118_64 |
BXD90 |
F |
CTL |
hippocampus |
|
223 |
S13118_65 |
BXD90 |
F |
CTL |
hippocampus |
|
224 |
S13118_7 |
BXD101 |
F |
CTL |
hippocampus |
|
225 |
S31218_01 |
BXD32 |
F |
CTL |
hippocampus |
|
226 |
S31218_02 |
BXD32 |
F |
CTL |
hippocampus |
|
227 |
S31218_04 |
BXD44 |
F |
CTL |
hippocampus |
|
228 |
S31218_15 |
BXD63 |
F |
CTL |
hippocampus |
|
229 |
S31218_16 |
BXD63 |
F |
CTL |
hippocampus |
|
230 |
S31218_18 |
BXD65b |
F |
CTL |
hippocampus |
|
231 |
S31218_1 |
BXD51 |
F |
CTL |
hippocampus |
|
232 |
S31218_21 |
BXD69 |
F |
CTL |
hippocampus |
|
233 |
S31218_22 |
BXD69 |
F |
CTL |
hippocampus |
|
234 |
S31218_24 |
BXD70 |
F |
CTL |
hippocampus |
|
235 |
S31218_25 |
BXD70 |
F |
CTL |
hippocampus |
|
236 |
S31218_27 |
BXD71 |
F |
CTL |
hippocampus |
|
237 |
S31218_28 |
BXD71 |
F |
CTL |
hippocampus |
|
238 |
S31218_31 |
BXD73a |
F |
CTL |
hippocampus |
|
239 |
S31218_35 |
BXD78 |
F |
CTL |
hippocampus |
|
240 |
S32718_01 |
DBA/2J |
F |
CTL |
hippocampus |
|
241 |
S32718_03 |
DBA/2J |
F |
CTL |
hippocampus |