Sample Processing: Samples were processed in the INIA Bioanalytical Core at the W. Harry Feinstone Center for Genomic Research, The University of Memphis, led by Thomas R. Sutter. All processing steps were performed by Shirlean Goodwin. In brief, RNA purity was evaluated using the 260/280 nm absorbance ratio, and values had to be greater than 1.8. The majority of samples were 1.9 to 2.1. RNA integrity was assessed using the Agilent Bioanalyzer 2100. We required an RNA integrity number (RIN) of greater than 8. This RIN value is based on the intensity ratio and amplitude of 18S and 28S rRNA signals. The standard Eberwine T7 polymerase method was used to catalyze the synthesis of cDNA template from polyA-tailed RNA using Superscript II reverse transcriptase (Invitrogen Inc.). The Enzo Life Sciences, Inc., BioArray High Yield RNA Transcript Labeling Kit (T7, Part No. 42655) was used to synthesize labeled cRNA. The cRNA was evaluated using both the 260/280 ratio (values of 2.0 or 2.1 are acceptable) and the Bioanalyzer output (a dark cRNA smear on the 2100 output centered roughly between 600 and 2000 nucleotides is required). Those samples that passed both QC steps (10% usually failed and new RNA samples had to be acquired and processed) were then sheared using a fragmentation buffer included in the Affymetrix GeneChip Sample Cleanup Module (Part No. 900371). Fragmented cRNA samples were either stored at -80 deg. C until use or were immediately injected onto the array. The arrays were hybridized and washed following standard Affymetrix protocols.
Replication and Sample Balance: We obtained a male sample pool and female sample pool from each isogenic group. While all strains were orginally represented by matched male and female samples, not all data sets passed the final quality control steps. Seventy-seven of 99 strains are represented by pairs or (rarely) trios of arrays. The first and last samples are technical replicates of a B6D2F1 hippocampal pool (aliquotes R1291H3 and R1291H4).
Experimental Design and Batch Structure: This data set consists arrays processed in six groups over a three month period (May 2005 to August 2005). Each group consists of 32 to 34 arrays.Sex, strain, and strain type (BXD, CXB, and MDP) were interleaved among groups to ensure reasonable balance and to minimize group-by-strain statistical confounds in group normalization. The two independent samples from a single strain were always run in different groups. All arrays were processed using a single protocol by a single operator, Shirlean Goodwin.
All samples in a group were labeled on one day, except for a few cases that failed QC on their first pass. The hybridization station accommodates up to 20 samples, and for this reason each group was split into a large first set of 20 samples and a second set of 12 to 14 samples. Samples were washed in groups of four and then held in at 4 deg C until all 20 (or 12-14) arrays were ready to scan. The last four samples out of the wash stations were scanned directly. Samples were scanned in sets of four.
Data Table 1:
This table lists all arrays by order of processing (Run), Sample ID, Strain, Sex, Age, number of animals in each sample pool (Pool), F generation number when less than 30 (GenN, and the Source of animals. SampleID is the ID number of the pooled RNA sample with a H1 through H3 suffix to indicate the actual hippocampal RNA aliquot used to prepare cRNA. Grp is the sequential group processing number (1 - 6).
index tube ID strain age sex batch ID pool sizel RMA outlier scale factor back ground average present absent marginal AFFX-b-ActinMur (3'/5') AFFX-GapdhMur (3'/5') source 1 R2028H2 129S1/SvImJ 66 F 5 3 0.1 4.362 64.49 0.497 0.484 0.019 2.78 1.13 JAX 2 R2029H2 129S1/SvImJ 66 M 6 3 0.04 5.208 41.21 0.49 0.49 0.02 1.62 0.95 JAX 3 R2030H1 A/J 57 M 5 2 0.06 3.307 45.16 0.527 0.454 0.018 1.63 0.99 UTM RW 4 R2032H3 AKR/J 66 F 5 3 0.04 3.054 61.03 0.51 0.471 0.018 1.46 0.79 JAX 5 R2454H1 AKR/J 66 M 6 4 0.11 2.892 58.55 0.474 0.507 0.019 1.99 0.78 JAX 6 R1289H2 B6D2F1 64 F 6 3 0.02 2.406 53.84 0.492 0.489 0.019 1.61 0.96 UTM RW 7 R1291H3 B6D2F1 66 M 1 3 0.01 3.524 48.54 0.487 0.494 0.019 1.21 1.52 UTM RW 8 R1291H4 B6D2F1 66 M 6 3 0.08 3.891 46.69 0.512 0.469 0.019 1.9 0.89 UTM RW 9 R2036H3 BALB/cJ 51 F 5 3 0.12 2.611 56.29 0.518 0.466 0.017 3.3 1.23 UTM RW 10 R2053H1 BALB/cJ 55 M 5 3 0.1 2.505 63.27 0.499 0.483 0.018 3.1 1.34 UTM RW 11 R2037H2 BALB/cJ 51 M 6 4 0.01 2.546 58.13 0.497 0.485 0.018 1.26 0.77 UTM RW 12 R1507H1 BXD1 58 M 3 3 0.02 4.056 60.17 0.478 0.503 0.019 1.15 0.76 Glenn 13 R1520H1 BXD2 56 F 4 4 0.09 1.715 71.62 0.515 0.467 0.018 2.36 1.6 Glenn 14 R1516H1 BXD2 61 M 1 4 0.01 2.231 64.86 0.508 0.474 0.019 1.3 1.53 Glenn 15 R1593H2 BXD5 60 F 1 4 0 1.913 59.96 0.487 0.493 0.02 0.98 1.44 Glenn 16 R1692H1 BXD5 60 M 3 2 0.07 3.764 72.74 0.465 0.516 0.02 1.15 0.74 Glenn 17 R1539H2 BXD6 59 F 1 4 0 2.488 54.97 0.518 0.463 0.018 1.08 1.33 Glenn 18 R1538H1 BXD6 59 M 4 3 0.01 2.585 50.27 0.505 0.475 0.02 1.46 0.79 Glenn 19 R1518H1 BXD8 56 F 1 3 0 2.92 54.84 0.515 0.465 0.02 1.32 1.24 Glenn 20 R1548H1 BXD8 59 M 6 3 0.07 2.132 59.37 0.504 0.477 0.019 2.16 1.54 Glenn 21 R1350H2 BXD9 86 F 1 3 0.05 2.771 60.62 0.5 0.482 0.018 1.01 1.28 UMemphis 22 R1531H1 BXD11 56 F 6 3 0.06 2.229 56.36 0.505 0.475 0.02 2.23 1.02 Glenn 23 R1367H1 BXD11 56 M 1 3 0.01 2.11 78.78 0.503 0.477 0.02 1.07 1.27 Glenn 24 R1530H1 BXD12 58 F 1 3 0 3.227 53.77 0.505 0.477 0.018 0.95 1.4 Glenn 25 R1529H1 BXD13 58 F 6 3 0.05 2.55 59.05 0.497 0.485 0.018 2 1.54 Glenn 26 R1662H2 BXD13 60 M 1 3 0.03 4.603 45.81 0.509 0.472 0.019 1.3 0.82 Glenn 27 R1524H1 BXD15 60 F 6 4 0.02 2.961 50.93 0.497 0.484 0.019 1.74 0.91 Glenn 28 R1515H1 BXD15 61 M 1 3 0.01 3.316 57.05 0.503 0.478 0.019 1.32 1.21 Glenn 29 R1661H1 BXD16 61 F 1 3 0.01 2.778 59.81 0.516 0.466 0.019 1.39 1.2 Glenn 30 R1594H1 BXD16 61 M 4 3 0.03 2.634 53.66 0.504 0.478 0.018 1.96 1.51 Glenn 31 R1471H1 BXD19 157 M 1 3 0.02 3.165 43.34 0.519 0.462 0.018 1.01 1.29 UTM JB 32 R1573H1 BXD20 59 F 1 3 0.02 3.749 52.7 0.513 0.469 0.018 1.01 1.27 Glenn 33 R2507H1 BXD20 60 M 6 3 0.06 3.568 57 0.472 0.508 0.02 1.29 0.76 Glenn 34 R1347H2 BXD21 64 F 1 4 0.01 2.881 61.49 0.494 0.486 0.019 0.92 1.22 UMemphis 35 R1337H2 BXD21 102 F 2 4 0 2.673 58.05 0.492 0.489 0.019 1.4 0.76 UAB 36 R1848H3 BXD22 196 F 6 4 0.02 2.943 51.7 0.494 0.485 0.021 2.2 0.78 UAB 37 R1525H1 BXD22 59 M 2 3 0.02 2.248 55.76 0.548 0.433 0.018 1.26 0.74 Glenn 38 R1280H2 BXD23 56 F 1 3 0.01 3.187 54.63 0.458 0.523 0.019 0.96 1.2 UTM RW 39 R1537H1 BXD23 58 F 5 3 0.1 3.719 67.54 0.468 0.513 0.019 1.51 0.96 Glenn 40 R1343H2 BXD24 71 F 2 3 0.01 2.083 65.07 0.506 0.474 0.019 1.46 0.75 UMemphis 41 R1517H1 BXD24 57 M 3 3 0.01 3.471 53.66 0.504 0.476 0.019 1.28 0.78 Glenn 42 R1366H1 BXD27 60 F 2 4 0 2.26 48.46 0.518 0.463 0.019 1.29 0.77 Glenn 43 R1849H1 BXD27 70 M 5 3 0.06 8.801 38.34 0.468 0.512 0.019 2.42 1.08 UAB 44 R1353H1 BXD28 79 F 3 4 0.01 3.22 76.22 0.48 0.5 0.02 1.33 0.78 UMemphis 45 R2332H1 BXD28 60 M 2 3 0.01 3.217 63.68 0.491 0.49 0.019 1.37 0.79 Glenn 46 R1532H1 BXD29 57 F 2 3 0.01 2.122 59.18 0.524 0.456 0.019 1.17 0.76 Glenn 47 R1356H1 BXD29 76 M 5 3 0.01 4.033 47.67 0.52 0.463 0.017 1.17 0.78 UMemphis 48 R1240H2 BXD31 61 M 2 3 0.02 2.335 65.17 0.507 0.474 0.019 1.31 0.78 UTM RW 49 R1508H2 BXD32 58 M 2 4 0.01 1.917 67.78 0.539 0.442 0.019 1.28 0.73 Glenn 50 R1345H3 BXD33 65 F 2 2 0.01 2.098 63.14 0.522 0.459 0.019 1.27 0.73 UMemphis 51 R1581H1 BXD33 59 M 3 3 0.01 3.229 53.16 0.496 0.485 0.019 1.19 0.78 Glenn 52 R1527H1 BXD34 59 F 2 3 0.01 2.3 58.92 0.51 0.471 0.019 1.24 0.76 Glenn 53 R1339H3 BXD34 74 M 5 3 0.12 2.888 53.49 0.506 0.476 0.018 2.39 1.35 UMemphis 54 R1469H1 BXD36 83 F 3 3 0.02 3.473 49.9 0.494 0.486 0.02 1.11 0.76 UMemphis 55 R1363H1 BXD36 77 M 2 4 0.01 2.184 48.19 0.538 0.443 0.02 1.28 0.77 UMemphis 56 R1855H1 BXD38 55 F 3 4 0.01 3.536 54.54 0.49 0.492 0.018 1.39 0.75 Glenn 57 R1510H1 BXD38 59 M 2 3 0.01 2.186 68.06 0.521 0.46 0.019 1.26 0.79 Glenn 58 R1528H2 BXD39 59 F 2 3 0.03 4.717 38.3 0.511 0.47 0.02 1.12 0.75 Glenn 59 R1514H1 BXD39 59 M 3 3 0.03 3.992 56.06 0.477 0.504 0.019 1.43 0.81 Glenn 60 R1522H1 BXD40 59 F 4 4 0 2.631 67.16 0.49 0.491 0.018 1.56 0.77 Glenn 61 R1359H1 BXD40 73 M 2 3 0.09 7.458 39.86 0.451 0.527 0.021 1.28 0.74 UMemphis 62 R1334H2 BXD43 59 F 1 3 0 2.672 54.36 0.492 0.491 0.017 1.2 2.06 UTM RW 63 R1303H1 BXD43 63 M 3 4 0.02 3.497 51.9 0.486 0.495 0.019 1.15 0.8 UTM RW 64 R1326H1 BXD44 65 F 3 4 0 3.412 53.96 0.496 0.485 0.018 1.35 0.78 UTM RW 65 R1577H2 BXD44 56 M 1 3 0.02 2.159 67.52 0.512 0.469 0.019 1.18 1.71 UTM RW 66 R1316H1 BXD48 58 F 4 3 0 2.445 68.59 0.515 0.467 0.019 1.16 0.73 UTM RW 67 R1575H3 BXD48 65 M 3 4 0.05 4.577 55.78 0.466 0.514 0.019 1.59 0.9 UTM RW 68 R2521H1 BXD50 63 F 6 4 0.01 3.109 57.28 0.495 0.485 0.02 1.23 0.78 UTM RW 69 R1944H2 BXD50 81 M 1 3 0.01 2.546 63.39 0.495 0.485 0.02 0.9 1.57 UTM RW 70 R2331H1 BXD51 66 F 3 3 0.03 3.534 44.42 0.501 0.481 0.017 1.2 0.9 UTM RW 71 R1582H1 BXD51 71 M 6 4 0.03 2.92 47.87 0.489 0.491 0.02 1.36 0.75 UTM RW 72 R1331H1 BXD60 60 F 4 3 0.01 2.867 50.33 0.492 0.487 0.021 1.34 0.78 UTM RW 73 R1281H2 BXD60 59 M 1 3 0 2.39 58.44 0.511 0.469 0.02 0.94 1.2 UTM RW 74 R1856H2 BXD61 94 M 1 2 0 3.502 49.6 0.501 0.48 0.019 0.96 1.3 UTM RW 75 R1246H1 BXD62 54 F 1 4 0.02 3.405 51.47 0.511 0.471 0.018 1.14 1.34 UTM RW 76 R1585H2 BXD62 64 M 6 4 0.01 3.156 55.77 0.518 0.464 0.018 1.43 0.82 UTM RW 77 R1945H1 BXD63 107 F 1 3 0.02 2.811 52.65 0.522 0.459 0.019 1.05 1.36 UTM RW 78 R2093H3 BXD63 70 M 6 3 0.02 3.894 42.85 0.503 0.477 0.019 1.29 1.01 UTM RW 79 R2062H2 BXD64 65 F 1 3 0.05 3.795 78.48 0.513 0.468 0.019 0.98 1.43 UTM RW 80 R2061H1 BXD64 87 M 3 4 0.01 3.536 61.57 0.477 0.504 0.019 1.31 0.78 UTM RW 81 R2054H2 BXD65 55 F 1 2 0.03 3.159 80.96 0.48 0.502 0.018 1.09 1.24 UTM RW 82 R2056H2 BXD65 89 M 6 2 0 2.836 59.6 0.504 0.477 0.019 1.3 0.75 UTM RW 83 R1941H2 BXD66 78 F 1 4 0.01 2.734 50.93 0.499 0.481 0.02 1.18 1.29 UTM RW 84 R1949H2 BXD66 96 M 4 2 0.04 2.828 51.27 0.474 0.508 0.019 2.05 1.12 UTM RW 85 R2060H1 BXD67 54 F 6 3 0.01 2.561 43.88 0.502 0.479 0.02 1.7 0.84 UTM RW 86 R2052H1 BXD67 61 M 1 4 0.01 3.161 43.23 0.521 0.46 0.018 1.09 1.31 UTM RW 87 R2074H1 BXD68 60 F 5 3 0.02 6.528 49.62 0.479 0.502 0.019 1.48 0.83 UTM RW 88 R1928H1 BXD68 72 M 2 2 0.01 2.404 48.28 0.521 0.459 0.02 1.3 0.74 UTM RW 89 R1439H3 BXD69 60 F 2 3 0.02 2.463 59.14 0.522 0.459 0.018 1.31 0.78 UTM RW 90 R1559H1 BXD69 64 M 3 3 0.03 2.987 67.74 0.486 0.496 0.017 1.38 0.8 UTM RW 91 R2134H1 BXD70 64 F 5 2 0.02 2.148 58.64 0.532 0.45 0.019 1.4 0.85 UTM RW 92 R2063H1 BXD70 55 M 2 3 0.02 3.481 55.32 0.513 0.469 0.018 1.28 0.71 UTM RW 93 R1277H1 BXD73 60 F 4 2 0.01 2.576 62.45 0.502 0.479 0.019 1.35 0.79 UTM RW 94 R1443H2 BXD73 76 M 2 3 0.01 2.312 64.34 0.499 0.481 0.02 1.48 0.77 UTM RW 95 R2055H2 BXD74 79 M 2 3 0.01 2.576 56.84 0.509 0.473 0.018 1.46 0.88 UTM RW 96 R2316H1 BXD74 193 M 5 2 0.01 3.457 55.35 0.508 0.471 0.02 1.17 0.78 UTM RW 97 R1871H1 BXD75 61 F 2 3 0.04 1.723 56.4 0.53 0.451 0.019 1.3 0.76 UTM RW 98 R1844H2 BXD75 90 M 3 4 0.01 1.934 56.23 0.52 0.461 0.019 1.62 0.86 UTM RW 99 R1948H2 BXD76 81 F 2 3 0.01 1.507 68.85 0.553 0.428 0.02 1.3 0.75 UTM RW 100 R2094H1 BXD76 61 M 5 4 0.01 3.299 42.69 0.519 0.462 0.019 1.39 0.88 UTM RW 101 R2262H1 BXD77 62 F 3 4 0.02 4.317 47.16 0.493 0.488 0.019 1.32 0.74 UTM RW 102 R1423H1 BXD77 62 M 2 3 0.02 3.071 54.15 0.51 0.471 0.019 1.26 0.74 UTM RW 103 R1947H1 BXD79 108 F 2 2 0.01 2.599 51.52 0.524 0.457 0.019 1.35 0.74 UTM RW 104 R2092H1 BXD79 86 M 5 4 0.06 3.735 42.25 0.514 0.468 0.018 2.94 1.06 UTM RW 105 R1880H1 BXD80 68 F 5 3 0.06 4.855 42.22 0.501 0.481 0.018 2.17 1.36 UTM RW 106 R1881H2 BXD80 68 M 2 3 0.02 2.073 48.93 0.524 0.458 0.019 1.34 0.83 UTM RW 107 R2075H1 BXD83 60 F 2 3 0.01 2.454 55.1 0.502 0.48 0.018 1.27 0.77 UTM RW 108 R2076H2 BXD83 60 M 6 3 0.03 2.624 55.65 0.495 0.488 0.018 2.21 0.94 UTM RW 109 R2077H2 BXD84 62 F 6 2 0 2.1 71.87 0.522 0.459 0.018 1.68 0.81 UTM RW 110 R2135H3 BXD84 75 M 2 2 0.01 2.467 64.46 0.505 0.476 0.019 1.2 0.74 UTM RW 111 R1473H1 BXD85 79 F 2 3 0.02 3.384 55.34 0.478 0.502 0.02 1.24 0.77 UTM RW 112 R1474H1 BXD85 57 M 1 3 0.01 2.831 55.24 0.522 0.461 0.018 1.04 1.29 UTM RW 113 R1597H1 BXD85 86 M 4 4 0.09 2.028 53.95 0.487 0.492 0.021 1.28 0.83 UTM RW 114 R1415H1 BXD86 77 F 4 3 0.02 2.525 53.16 0.495 0.485 0.02 1.66 0.91 UTM RW 115 R1710H1 BXD87 84 M 2 4 0.01 2.697 56.4 0.512 0.469 0.019 1.28 0.79 UTM RW 116 R1872H2 BXD89 90 F 2 2 0.02 3.013 63.53 0.492 0.488 0.021 1.22 0.72 UTM RW 117 R1850H3 BXD89 82 M 4 4 0.03 2.736 44.89 0.498 0.483 0.019 1.5 0.83 UTM RW 118 R2058H1 BXD90 61 F 2 3 0.01 3.389 48.05 0.502 0.478 0.02 1.53 0.76 UTM RW 119 R1301H2 BXD92 58 F 2 3 0.02 3.543 41.97 0.522 0.46 0.018 1.5 0.79 UTM RW 120 R1309H1 BXD92 59 M 4 3 0.05 1.655 66.34 0.498 0.481 0.021 1.52 0.82 UTM RW 121 R2057H1 BXD93 92 F 5 3 0.02 4.033 44.41 0.509 0.471 0.02 1.22 0.78 UTM RW 122 R2059H1 BXD93 58 M 1 3 0 3.058 60.29 0.493 0.488 0.019 1.18 1.37 UTM RW 123 R2313H1 BXD94 59 F 3 3 0 3.091 59.45 0.487 0.495 0.018 1.34 0.73 UTM RW 124 R1915H1 BXD96 65 F 5 2 0.04 5.145 46.19 0.502 0.481 0.017 1.37 0.74 UTM RW 125 R1846H2 BXD96 63 M 1 3 0 3.159 55.85 0.487 0.493 0.02 0.92 1.26 UTM RW 126 R1927H2 BXD97 67 M 1 3 0.04 2.622 57.81 0.539 0.444 0.017 1.45 1.32 UTM RW 127 R1942H1 BXD98 62 F 5 3 0.04 3.104 48.42 0.528 0.454 0.019 2.22 1.08 UTM RW 128 R1943H2 BXD98 62 M 3 3 0.02 4.04 56.85 0.484 0.497 0.019 1.18 0.76 UTM RW 129 R2197H1 BXD99 70 F 3 3 0.02 4.288 51.75 0.49 0.492 0.018 1.35 0.81 UTM RW 130 R2315H1 BXD99 84 M 5 2 0.03 6.036 43.05 0.484 0.497 0.018 1.7 0.96 UTM RW 131 R2038H3 C3H/HeJ 63 F 6 3 0.02 2.671 66.74 0.476 0.504 0.02 1.41 0.77 UTM RW 132 R2039H1 C3H/HeJ 63 M 5 3 0.1 3.384 44.15 0.528 0.454 0.017 2.16 0.88 UTM RW 133 R2137H1 C57BL/6ByJ 55 F 5 3 0.02 4.746 47.01 0.488 0.493 0.018 1.23 0.79 JAX 134 R1361H1 C57BL/6J 69 F 6 4 0.01 3.058 51.87 0.477 0.503 0.02 1.67 0.76 UTM RW 135 R2041H2 C57BL/6J 65 M 1 4 0.04 3.341 49.26 0.527 0.456 0.018 1.14 1.45 UTM RW 136 R1449H2 C57BL/6J 71 M 5 3 0.09 3.592 44.32 0.47 0.51 0.02 1.68 0.77 UTM DG 137 R2619H1 CAST/Ei 64 F 5 3 0.14 4.077 51.87 0.455 0.528 0.018 2.74 1.2 JAX 138 R2116H1 CXB1 55 F 3 3 0.07 5.792 51.59 0.459 0.521 0.02 1.17 0.8 JAX 139 R2096H1 CXB1 55 M 4 2 0.01 3.435 53.78 0.495 0.485 0.02 1.22 0.79 JAX 140 R2124H1 CXB10 53 F 4 2 0.11 4.867 39.88 0.451 0.528 0.02 1.55 0.8 JAX 141 R2125H1 CXB11 58 F 3 3 0.03 3.256 54.95 0.461 0.519 0.02 1.46 0.77 JAX 142 R2128H1 CXB11 58 M 4 2 0.06 4.986 54.13 0.465 0.515 0.02 1.11 0.83 JAX 143 R2126H1 CXB12 47 F 4 3 0.11 3.935 54.11 0.469 0.511 0.021 1.5 0.79 JAX 144 R2109H1 CXB12 47 M 3 3 0.07 4.518 49.26 0.488 0.492 0.02 1.23 0.77 JAX 145 R2110H1 CXB13 56 M 4 3 0.21 3.478 48.08 0.461 0.517 0.022 1.21 0.78 JAX 146 R2117H2 CXB2 62 F 4 2 0.04 3.39 45.97 0.533 0.45 0.017 2.05 0.89 JAX 147 R2098H1 CXB2 68 M 3 3 0.02 2.572 54.22 0.496 0.485 0.019 1.38 0.86 JAX 148 R2118H1 CXB3 47 F 3 3 0.03 3.646 63.16 0.478 0.503 0.019 1.22 0.77 JAX 149 R2100H1 CXB3 47 M 4 3 0.02 5.76 51.38 0.48 0.503 0.017 1.24 0.81 JAX 150 R2119H1 CXB4 58 F 4 3 0.02 3.897 49.21 0.488 0.494 0.018 1.31 0.79 JAX 151 R2101H1 CXB4 58 M 3 3 0.13 7.372 53.77 0.433 0.548 0.019 1.2 0.97 JAX 152 R2505H1 CXB5 80 F 6 3 0.02 2.83 49.6 0.499 0.48 0.02 1.33 0.76 UTM RW 153 R2131H1 CXB5 42 M 4 3 0.1 5.577 51.15 0.434 0.547 0.019 1.7 0.89 JAX 154 R0129H2 CXB5 70 M 3 3 0.07 4.829 45.42 0.488 0.493 0.019 1.23 0.83 UTM RW 155 R2102H1 CXB6 49 M 4 3 0.07 5.148 51.63 0.453 0.529 0.018 1.43 0.87 JAX 156 R2121H1 CXB7 63 F 4 2 0.06 4.904 48.71 0.464 0.517 0.019 1.19 0.92 JAX 157 R2104H2 CXB7 58 M 3 2 0.06 3.389 48.79 0.502 0.479 0.019 1.74 1.48 JAX 158 R2122H1 CXB8 54 F 3 3 0.04 4.128 59.77 0.451 0.529 0.02 1.12 0.76 JAX 159 R2105H1 CXB8 41 M 4 3 0.16 3.146 61.04 0.451 0.53 0.019 1.34 0.84 JAX 160 R2123H1 CXB9 54 F 3 3 0.08 5.708 55.94 0.438 0.543 0.019 1.32 0.78 JAX 161 R2106H1 CXB9 54 M 4 3 0.06 5.868 46.55 0.469 0.512 0.019 1.18 0.82 JAX 162 R2045H2 D2B6F1 65 F 1 2 0.01 4.403 47.99 0.497 0.485 0.018 1.09 1.53 UTM RW 163 R1595H2 D2B6F1 63 F 5 3 0.06 2.579 58.49 0.506 0.475 0.019 2.49 1.21 UTM RW 164 R1551H1 D2B6F1 72 F 6 3 0.02 2.62 53.76 0.506 0.476 0.018 1.37 0.76 UTM RW 165 R1468H1 DBA/2J 64 F 5 3 0.03 2.929 53.8 0.515 0.465 0.019 1.28 0.79 UTM RW 166 R1683H1 KK/HIJ 72 F 6 3 0.02 3.919 54.23 0.491 0.489 0.02 1.31 0.83 JAX 167 R1687H3 KK/HIJ 72 M 5 3 0.04 3.888 40.86 0.499 0.483 0.019 1.86 0.88 JAX 168 R2046H1 LG/J 63 F 5 2 0.03 2.822 59.18 0.514 0.468 0.018 1.68 0.8 UTM RW 169 R2047H2 LG/J 63 M 6 3 0.07 2.038 60.34 0.509 0.471 0.02 2.16 0.95 UTM RW 170 R2048H1 NOD/LtJ 77 F 6 2 0.14 4.045 50.21 0.489 0.49 0.021 2.89 0.95 UTM RW 171 R2049H3 NOD/LtJ 76 M 5 3 0.1 2.328 52.78 0.519 0.462 0.019 3.09 1.35 UTM RW 172 R2200H1 NZO/HlLtJ 62 F 5 2 0.03 2.648 54.29 0.543 0.438 0.019 1.27 0.8 JAX 173 R2350H1 NZO/HlLtJ 96 M 6 2 0.19 2.391 50.52 0.518 0.463 0.02 3.71 2.21 JAX 174 R2051H3 PWD/PhJ 64 M 5 3 0.07 3.266 51.5 0.475 0.506 0.019 2.8 1.01 UTM RW 175 R2322H1 PWK/PhJ 63 F 5 2 0.09 2.94 54.91 0.511 0.47 0.019 2.32 1.02 JAX 176 R2349H1 PWK/PhJ 83 M 6 2 0.15 3.306 54.93 0.459 0.522 0.019 4.65 1.45 JAX 177 R2198H2 WSB/EiJ 58 F 6 1 0.02 2.922 57.97 0.502 0.479 0.019 1.44 0.76 JAX 178 R2199H1 WSB/EiJ 58 M 5 3 0.04 3.171 54.95 0.475 0.505 0.02 1.32 0.81 JAX