About amplification and hybridization:

Total RNA was quantified using RiboGreen and split into equal aliquots of approximately 10 ng, representing RNA from approximately 10,000 cells, and labeled using a total of three rounds of RNA amplification, exactly as described previously (Scherer et al. 2003). Labeled cRNA was fractionated and hybridized to the U74Av2 microarray following standard Affymetrix protocols.

About the chromosome and megabase position values:

The chromosomal locations of probe sets and gene markers were determined by BLAT analysis using the Mouse Genome Sequencing Consortium Oct 2003 Assembly (see http://genome.ucsc.edu/). We thank Yan Cui (UTHSC) for allowing us to use his Linux cluster to perform this analysis.