Tissue preparation protocol. Animal were killed by rapid cervical dislocation. Retinas were removed immediately and placed in RiboLock (RiboLock: Thermo Scientific RiboLock RNase #EO0381 40U/µl 2500U) at room temperature. Individual retinas from the mouse were stored in each tube. RNA was isolated using a Qiacube with the resultant RIN scores ranging from 8.5 to 10.0.