The study includes 137 mice (11~25 months old) from 73 strains (B6, D2, DBF1, and 70 BXD strains). All procedures were approved by the UTHSC Institutional Animal Care and Use Committee.
- -The table of samples that are finally used for this study:
- -| Index | -Strain | -Sex | -RNA ID | -Age | -Phase | -Tissue | -
| 1 | -BXD1 | -F | -R7281H | -448 | -II | -Hippocampus | -
| 2 | -BXD1 | -M | -R7276H | -323 | -II | -Hippocampus | -
| 3 | -BXD2 | -M | -R7261H | -394 | -I | -Hippocampus | -
| 4 | -BXD2 | -F | -R7256H | -456 | -I | -Hippocampus | -
| 5 | -BXD6 | -M | -R7223H | -526 | -I | -Hippocampus | -
| 6 | -BXD8 | -M | -R7203H | -471 | -I | -Hippocampus | -
| 7 | -BXD8 | -F | -R7200H | -460 | -I | -Hippocampus | -
| 8 | -BXD8 | -F | -R7198H | -433 | -I | -Hippocampus | -
| 9 | -BXD9 | -F | -R7294H | -456 | -II | -Hippocampus | -
| 10 | -BXD11 | -M | -R7300H | -457 | -II | -Hippocampus | -
| 11 | -BXD11 | -F | -R7227H | -536 | -I | -Hippocampus | -
| 12 | -BXD12 | -F | -R7183H | -506 | -I | -Hippocampus | -
| 13 | -BXD12 | -F | -R7233H | -561 | -I | -Hippocampus | -
| 14 | -BXD12 | -M | -R7234H | -606 | -I | -Hippocampus | -
| 15 | -BXD14 | -F | -R7238H | -605 | -I | -Hippocampus | -
| 16 | -BXD14 | -M | -R7235H | -605 | -I | -Hippocampus | -
| 17 | -BXD16 | -F | -R7236H | -561 | -I | -Hippocampus | -
| 18 | -BXD16 | -M | -R7239H | -475 | -I | -Hippocampus | -
| 19 | -BXD18 | -F | -R7229H | -493 | -I | -Hippocampus | -
| 20 | -BXD19 | -F | -R7267H | -551 | -I | -Hippocampus | -
| 21 | -BXD19 | -M | -R7268H | -492 | -I | -Hippocampus | -
| 22 | -BXD20 | -M | -R7232H | -506 | -I | -Hippocampus | -
| 23 | -BXD20 | -M | -R7263H | -489 | -I | -Hippocampus | -
| 24 | -BXD21 | -F | -R7230H | -537 | -I | -Hippocampus | -
| 25 | -BXD22 | -F | -R7260H | -502 | -I | -Hippocampus | -
| 26 | -BXD22 | -M | -R7262H | -596 | -I | -Hippocampus | -
| 27 | -BXD23 | -F | -R7258H | -502 | -I | -Hippocampus | -
| 28 | -BXD23 | -M | -R7257H | -462 | -I | -Hippocampus | -
| 29 | -BXD24 | -M | -R7231H | -470 | -I | -Hippocampus | -
| 30 | -BXD24 | -F | -R7228H | -415 | -I | -Hippocampus | -
| 31 | -BXD24 | -F | -R7255H | -456 | -I | -Hippocampus | -
| 32 | -BXD25 | -F | -R7252H | -454 | -I | -Hippocampus | -
| 33 | -BXD27 | -F | -R7286H | -472 | -II | -Hippocampus | -
| 34 | -BXD27 | -F | -R7170H | -472 | -I | -Hippocampus | -
| 35 | -BXD28 | -M | -R7254H | -493 | -I | -Hippocampus | -
| 36 | -BXD28 | -F | -R7251H | -543 | -I | -Hippocampus | -
| 37 | -BXD29 | -F | -R7259H | -483 | -I | -Hippocampus | -
| 38 | -BXD33 | -M | -R7253H | -464 | -I | -Hippocampus | -
| 39 | -BXD33 | -F | -R7174H | -471 | -I | -Hippocampus | -
| 40 | -BXD33 | -F | -R7244H | -448 | -I | -Hippocampus | -
| 41 | -BXD33 | -M | -R7270H | -662 | -I | -Hippocampus | -
| 42 | -BXD38 | -F | -R7242H | -464 | -I | -Hippocampus | -
| 43 | -BXD38 | -M | -R7247H | -446 | -I | -Hippocampus | -
| 44 | -BXD39 | -M | -R7250H | -536 | -I | -Hippocampus | -
| 45 | -BXD39 | -F | -R7173H | -500 | -I | -Hippocampus | -
| 46 | -BXD39 | -M | -R7175H | -500 | -I | -Hippocampus | -
| 47 | -BXD40 | -F | -R7288H | -451 | -II | -Hippocampus | -
| 48 | -BXD40 | -M | -R7210H | -470 | -I | -Hippocampus | -
| 49 | -BXD40 | -M | -R7197H | -614 | -I | -Hippocampus | -
| 50 | -BXD42 | -F | -R7280H | -518 | -II | -Hippocampus | -
| 51 | -BXD42 | -M | -R7246H | -446 | -I | -Hippocampus | -
| 52 | -BXD42 | -F | -R7266H | -486 | -I | -Hippocampus | -
| 53 | -BXD43 | -F | -R7249H | -454 | -I | -Hippocampus | -
| 54 | -BXD43 | -M | -R7248H | -462 | -I | -Hippocampus | -
| 55 | -BXD44 | -M | -R7241H | -415 | -I | -Hippocampus | -
| 56 | -BXD44 | -M | -R7279H | -419 | -II | -Hippocampus | -
| 57 | -BXD44 | -F | -R7243H | -438 | -I | -Hippocampus | -
| 58 | -BXD45 | -F | -R7176H | -451 | -I | -Hippocampus | -
| 59 | -BXD48 | -F | -R7245H | -499 | -I | -Hippocampus | -
| 60 | -BXD48 | -M | -R7220H | -526 | -I | -Hippocampus | -
| 61 | -BXD48a | -F | -R7299H | -479 | -II | -Hippocampus | -
| 62 | -BXD48a | -M | -R7297H | -479 | -II | -Hippocampus | -
| 63 | -BXD50 | -F | -R7224H | -530 | -I | -Hippocampus | -
| 64 | -BXD50 | -M | -R7221H | -530 | -I | -Hippocampus | -
| 65 | -BXD51 | -F | -R7177H | -487 | -I | -Hippocampus | -
| 66 | -BXD51 | -M | -R7290H | -407 | -II | -Hippocampus | -
| 67 | -BXD55 | -M | -R7222H | -528 | -I | -Hippocampus | -
| 68 | -BXD55 | -F | -R7225H | -587 | -I | -Hippocampus | -
| 69 | -BXD56 | -M | -R7178H | -501 | -I | -Hippocampus | -
| 70 | -BXD62 | -M | -R7291H | -439 | -II | -Hippocampus | -
| 71 | -BXD63 | -M | -R7218H | -438 | -I | -Hippocampus | -
| 72 | -BXD63 | -F | -R7215H | -475 | -I | -Hippocampus | -
| 73 | -BXD64 | -M | -R7219H | -528 | -I | -Hippocampus | -
| 74 | -BXD64 | -F | -R7216H | -587 | -I | -Hippocampus | -
| 75 | -BXD65 | -F | -R7217H | -425 | -I | -Hippocampus | -
| 76 | -BXD65a | -F | -R7273H | -389 | -II | -Hippocampus | -
| 77 | -BXD65a | -M | -R7277H | -715 | -II | -Hippocampus | -
| 78 | -BXD65b | -M | -R7271H | -483 | -II | -Hippocampus | -
| 79 | -BXD66 | -M | -R7214H | -463 | -I | -Hippocampus | -
| 80 | -BXD66 | -F | -R7302H | -446 | -III | -Hippocampus | -
| 81 | -BXD67 | -F | -R7240H | -499 | -I | -Hippocampus | -
| 82 | -BXD67 | -M | -R7213H | -425 | -I | -Hippocampus | -
| 83 | -BXD67 | -F | -R7278H | -415 | -II | -Hippocampus | -
| 84 | -BXD68 | -M | -R7212H | -421 | -I | -Hippocampus | -
| 85 | -BXD68 | -F | -R7211H | -415 | -I | -Hippocampus | -
| 86 | -BXD69 | -F | -R7305H | -504 | -III | -Hippocampus | -
| 87 | -BXD70 | -F | -R7207H | -458 | -I | -Hippocampus | -
| 88 | -BXD70 | -M | -R7204H | -460 | -I | -Hippocampus | -
| 89 | -BXD71 | -M | -R7205H | -471 | -I | -Hippocampus | -
| 90 | -BXD71 | -F | -R7208H | -474 | -I | -Hippocampus | -
| 91 | -BXD73 | -F | -R7209H | -470 | -I | -Hippocampus | -
| 92 | -BXD73 | -M | -R7206H | -464 | -I | -Hippocampus | -
| 93 | -BXD73a | -F | -R7181H | -443 | -I | -Hippocampus | -
| 94 | -BXD73a | -M | -R7182H | -614 | -I | -Hippocampus | -
| 95 | -BXD76 | -M | -R7179H | -579 | -I | -Hippocampus | -
| 96 | -BXD76 | -F | -R7188H | -408 | -I | -Hippocampus | -
| 97 | -BXD76 | -M | -R7187H | -564 | -I | -Hippocampus | -
| 98 | -BXD77 | -M | -R7292H | -347 | -II | -Hippocampus | -
| 99 | -BXD77 | -F | -R7201H | -454 | -I | -Hippocampus | -
| 100 | -BXD79 | -M | -R7202H | -485 | -I | -Hippocampus | -
| 101 | -BXD79 | -F | -R7199H | -515 | -I | -Hippocampus | -
| 102 | -BXD79 | -M | -R7298H | -704 | -II | -Hippocampus | -
| 103 | -BXD81 | -M | -R7196H | -515 | -I | -Hippocampus | -
| 104 | -BXD81 | -F | -R7190H | -458 | -I | -Hippocampus | -
| 105 | -BXD83 | -M | -R7184H | -441 | -I | -Hippocampus | -
| 106 | -BXD84 | -M | -R7195H | -474 | -I | -Hippocampus | -
| 107 | -BXD84 | -M | -R7296H | -484 | -II | -Hippocampus | -
| 108 | -BXD84 | -F | -R7192H | -522 | -I | -Hippocampus | -
| 109 | -BXD85 | -M | -R7272H | -425 | -II | -Hippocampus | -
| 110 | -BXD85 | -F | -R7193H | -506 | -I | -Hippocampus | -
| 111 | -BXD86 | -M | -R7191H | -425 | -I | -Hippocampus | -
| 112 | -BXD87 | -F | -R7194H | -425 | -I | -Hippocampus | -
| 113 | -BXD87 | -M | -R7303H | -478 | -III | -Hippocampus | -
| 114 | -BXD87 | -M | -R7186H | -442 | -I | -Hippocampus | -
| 115 | -BXD89 | -F | -R7295H | -446 | -II | -Hippocampus | -
| 116 | -BXD90 | -M | -R7287H | -434 | -II | -Hippocampus | -
| 117 | -BXD90 | -M | -R7293H | -558 | -II | -Hippocampus | -
| 118 | -BXD95 | -F | -R7180H | -467 | -I | -Hippocampus | -
| 119 | -BXD95 | -M | -R7169H | -467 | -I | -Hippocampus | -
| 120 | -BXD98 | -M | -R7237H | -605 | -I | -Hippocampus | -
| 121 | -BXD98 | -F | -R7171H | -639 | -I | -Hippocampus | -
| 122 | -BXD98 | -F | -R7275H | -488 | -II | -Hippocampus | -
| 123 | -BXD99 | -M | -R7189H | -524 | -I | -Hippocampus | -
| 124 | -BXD99 | -F | -R7172H | -471 | -I | -Hippocampus | -
| 125 | -BXD100 | -F | -R7282H | -463 | -II | -Hippocampus | -
| 126 | -BXD100 | -M | -R7283H | -507 | -II | -Hippocampus | -
| 127 | -BXD100 | -M | -R7274H | -577 | -II | -Hippocampus | -
| 128 | -BXD100 | -F | -R7226H | -464 | -I | -Hippocampus | -
| 129 | -BXD101 | -F | -R7284H | -490 | -II | -Hippocampus | -
| 130 | -BXD101 | -M | -R7285H | -408 | -II | -Hippocampus | -
| 131 | -C57BL/6J | -M | -R7264H | -489 | -I | -Hippocampus | -
| 132 | -C57BL/6J | -M | -R7289H | -756 | -II | -Hippocampus | -
| 133 | -D2B6F1 | -M | -R7265H | -492 | -I | -Hippocampus | -
| 134 | -D2B6F1 | -F | -R7304H | -495 | -III | -Hippocampus | -
| 135 | -DBA/2J | -F | -R7185H | -433 | -I | -Hippocampus | -
| 136 | -DBA/2J | -M | -R7269H | -367 | -I | -Hippocampus | -
| 137 | -DBA/2J | -F | -R7301H | -566 | -III | -Hippocampus | -
RNA Extraction
- -RNA was extracted using the RNeasy mini kit (Qiagen, Valencia, CA, USA) according to the manufactures’ procedure. 2100 BioAnalyzer (Agilent Technologies) was used to evaluate RNA integrity and quality. Samples with RNA Integrity Numbers (RIN values) > 8.0 were run on Affy MoGene1.0 ST at the UTHSC
diff --git a/general/datasets/UTHSC_BXD_AgeHipp0515/processing.rtf b/general/datasets/UTHSC_BXD_AgeHipp0515/processing.rtf deleted file mode 100644 index e951515..0000000 --- a/general/datasets/UTHSC_BXD_AgeHipp0515/processing.rtf +++ /dev/null @@ -1,3 +0,0 @@ -About data processing:
- -Raw microarray data were normalized using the Robust Multichip Array (RMA) method. The expression data were then re-normalized using a modified Z score.
diff --git a/general/datasets/UTHSC_BXD_AgeHipp0515/specifics.rtf b/general/datasets/UTHSC_BXD_AgeHipp0515/specifics.rtf deleted file mode 100644 index 3d4ba9a..0000000 --- a/general/datasets/UTHSC_BXD_AgeHipp0515/specifics.rtf +++ /dev/null @@ -1 +0,0 @@ -UTHSC BXD Aged Hippocampus Affy MoGene1.0 ST (May15) RMA Gene Level ** \ No newline at end of file diff --git a/general/datasets/UTHSC_BXD_AgeHipp0515/tissue.rtf b/general/datasets/UTHSC_BXD_AgeHipp0515/tissue.rtf deleted file mode 100644 index 3166717..0000000 --- a/general/datasets/UTHSC_BXD_AgeHipp0515/tissue.rtf +++ /dev/null @@ -1,3 +0,0 @@ -Tissue Harvest
- -The animals were sacrificed under saturated isoflurane. Hippocampus from the animals were dissected and stored at −80°C until RNA extraction.
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