From b2feda451ccfbeaed02dce9088d6dd228cf15861 Mon Sep 17 00:00:00 2001 From: Bonface Date: Tue, 13 Feb 2024 23:52:26 -0600 Subject: Update dataset RTF Files. --- general/datasets/UConn_RGC_RSeq_r_0918/specifics.rtf | 1 - general/datasets/UConn_RGC_RSeq_r_0918/summary.rtf | 3 --- 2 files changed, 4 deletions(-) delete mode 100644 general/datasets/UConn_RGC_RSeq_r_0918/specifics.rtf delete mode 100644 general/datasets/UConn_RGC_RSeq_r_0918/summary.rtf (limited to 'general/datasets/UConn_RGC_RSeq_r_0918') diff --git a/general/datasets/UConn_RGC_RSeq_r_0918/specifics.rtf b/general/datasets/UConn_RGC_RSeq_r_0918/specifics.rtf deleted file mode 100644 index 8c82808..0000000 --- a/general/datasets/UConn_RGC_RSeq_r_0918/specifics.rtf +++ /dev/null @@ -1 +0,0 @@ -Raw data \ No newline at end of file diff --git a/general/datasets/UConn_RGC_RSeq_r_0918/summary.rtf b/general/datasets/UConn_RGC_RSeq_r_0918/summary.rtf deleted file mode 100644 index aab94b8..0000000 --- a/general/datasets/UConn_RGC_RSeq_r_0918/summary.rtf +++ /dev/null @@ -1,3 +0,0 @@ -
Retinal ganglion cells (RGCs) convey the major output of information collected from the eye to the brain. Thirty subtypes of RGCs have been identified to date. Here, we analyze 6225 RGCs (average of 5000 genes per cell) from right and left eyes by single-cell RNA-seq and classify them into 40 subtypes using clustering algorithms. We identify additional subtypes and markers, as well as transcription factors predicted to cooperate in specifying RGC subtypes. Zic1, a marker of the right eye-enriched subtype, is validated by immunostaining in situ. Runx1 and Fst, the markers of other subtypes, are validated in purified RGCs by fluorescent in situ hybridization (FISH) and immunostaining. We show the extent of gene expression variability needed for subtype segregation, and we show a hierarchy in diversification from a cell-type population to subtypes. Finally, we present a website for comparing the gene expression of RGC subtypes.
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