From b2feda451ccfbeaed02dce9088d6dd228cf15861 Mon Sep 17 00:00:00 2001 From: Bonface Date: Tue, 13 Feb 2024 23:52:26 -0600 Subject: Update dataset RTF Files. --- general/datasets/Sa_m2_1104_g/experiment-design.rtf | 5 +++++ 1 file changed, 5 insertions(+) create mode 100644 general/datasets/Sa_m2_1104_g/experiment-design.rtf (limited to 'general/datasets/Sa_m2_1104_g/experiment-design.rtf') diff --git a/general/datasets/Sa_m2_1104_g/experiment-design.rtf b/general/datasets/Sa_m2_1104_g/experiment-design.rtf new file mode 100644 index 0000000..1b0f0b3 --- /dev/null +++ b/general/datasets/Sa_m2_1104_g/experiment-design.rtf @@ -0,0 +1,5 @@ +
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Animals were obtained from The Jackson Laboratory and housed for several weeks at BIDMC until they reached ~2 months of age (range from 55 to 62 days). Mice were killed by cervical dislocation and brains were removed and placed in RNAlater for 5 to 10 minutes prior to dissection. Cerebella and olfactory bulbs were removed; brains were hemisected, and both striata were dissected using a medial approach by Rosen that typically yields 5 to 7 mg of tissue per side. The purity of this dissection has been validated by an analysis of acetylcholinestase activity. A pool of dissected tissue from 3 or 4 adults (approximately 25-30 mg of tissue from 6 striata) of the same strain, sex, and age was collected in one session and used to generate cRNA samples. Rought 90 to 95% of all cells in the striatum are medium spiny neurons (Gerfen, 1992, for a review of the structure and function of the neostriatum).

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mRNA processing: We used the Amersham Biosciences cRNA synthesis kit protocol.

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