From b2feda451ccfbeaed02dce9088d6dd228cf15861 Mon Sep 17 00:00:00 2001 From: Bonface Date: Tue, 13 Feb 2024 23:52:26 -0600 Subject: Update dataset RTF Files. --- general/datasets/Inia_hyp_m_rma_1110/experiment-design.rtf | 6 ++++++ 1 file changed, 6 insertions(+) create mode 100644 general/datasets/Inia_hyp_m_rma_1110/experiment-design.rtf (limited to 'general/datasets/Inia_hyp_m_rma_1110/experiment-design.rtf') diff --git a/general/datasets/Inia_hyp_m_rma_1110/experiment-design.rtf b/general/datasets/Inia_hyp_m_rma_1110/experiment-design.rtf new file mode 100644 index 0000000..443af4a --- /dev/null +++ b/general/datasets/Inia_hyp_m_rma_1110/experiment-design.rtf @@ -0,0 +1,6 @@ +

Hypothalamus was dissected from adult male and female mice and process for expression analysis.

+ +

RNA isolation
+Total RNA was purified using the RNAeasy micro kit on the QIAcube system (www.qiagen.com). RNA purity and concentration was checked using 260/280 nm absorbance ratio and RNA integrity was analyzed using the Agilent Bioanalyzer 2100 (Agilent Technologies).

+ +

This preliminary data set is associated with 430 eQTLs with LOD scores above 10. Peak LRS is 146 for Trait ID 10513604 (Hdhd3).

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