From e34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534 Mon Sep 17 00:00:00 2001 From: Bonface Date: Thu, 15 Feb 2024 06:09:54 -0600 Subject: Update dataset RTF Files. --- general/datasets/INIA_Hyp_M_RMA_1110/cases.rtf | 916 ------------------------- 1 file changed, 916 deletions(-) delete mode 100644 general/datasets/INIA_Hyp_M_RMA_1110/cases.rtf (limited to 'general/datasets/INIA_Hyp_M_RMA_1110/cases.rtf') diff --git a/general/datasets/INIA_Hyp_M_RMA_1110/cases.rtf b/general/datasets/INIA_Hyp_M_RMA_1110/cases.rtf deleted file mode 100644 index f19e9c8..0000000 --- a/general/datasets/INIA_Hyp_M_RMA_1110/cases.rtf +++ /dev/null @@ -1,916 +0,0 @@ -

The BXD recombinant inbred strains are derived from crossing the C57BL/6J (B6) and DBA/2J (D2) parental strains and inbreeding for over 20 generations (Taylor et al., 1999; Peirce et al., 2004). All mice used in this study were born and housed at the University of Tennessee Health Science Center. Mice were kept at an average of 3–4 per cage in a temperature-controlled vivarium (22 deg C) and maintained at a 12 h light/dark cycle. All animal protocols were approved by the Animal Care and Use Committee. We studied a total of 50 BXD strains, but only acquired matched male–female data pairs for 39 strains (35 BXD strains, parental B6 and D2, and two reciprocal F1 hybrids, B6D2F1 and D2B6F1). The average age of mice was 78 days. We provide more detail on the experimen-tal design and precise age and time of sacrifice of all cases at stored in 4 deg C for 2–3 days. To dissect the hypothalamus, the brain was placed with the ventral side facing up in a coronal "brain cutting" matrix. Using the medial mammillary body as landmark, a vertical cut was made right along the posterior boundary of the hypothala-mus. A second vertical cut was made 2 mm from the first cut. This edge lies slightly caudal to the optic chiasm and cuts through the retrochiasmatic nucleus. The hypothalamus was then sliced out from this 2 mm thick section. Each of the 39 mouse strains is represented by male and female samples (total of 78 microarray samples). Each individual sample consisted of tissue pooled from two mice of the same strain and sex that are usually littermates. The total number of mice used was 78 females and 78 males. RNA was purified using the RNAeasy micro kit on the QIAcube system (Qiagen)2. RNA purity and concen-tration was checked using 260/280 nm absorbance ratio and RNA integrity was analyzed using the Agilent Bioanalyzer 2100 (Agilent Technologies).

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IndexArray IDTissueStrainAgeSexDate sacrificeTime sacrifice
1R6854HYPHypC57BL/6J77F8/18/108:45AM to 12:30 PM
2R6862HYPHypC57BL/6J77M8/18/108:45AM to 12:30 PM
3R6852HYPHypD2B6F177F8/18/108:45AM to 12:30 PM
4R6860HYPHypD2B6F177M8/18/108:45AM to 12:30 PM
5R6864HYPHypDBA/2J77F8/18/108:45AM to 12:30 PM
6R6866HYPHypDBA/2J68M8/18/108:45AM to 12:30 PM
7R6858HYPHypB6D2F169F8/18/108:45AM to 12:30 PM
8R6856HYPHypB6D2F169M8/18/108:45AM to 12:30 PM
9R6800HYPHypBXD171F8/17/101:15 PM to 5 PM
10R6796HYPHypBXD185M8/17/101:15 PM to 5 PM
11R6788HYPHypBXD1187F8/17/101:15 PM to 5 PM
12R6786HYPHypBXD1176M8/17/101:15 PM to 5 PM
13R6806HYPHypBXD1277M8/17/101:15 PM to 5 PM
14R6812HYPHypBXD1481F8/18/108:45AM to 12:30 PM
15R6804HYPHypBXD2485F8/17/101:15 PM to 5 PM
16R6792HYPHypBXD2775F8/17/101:15 PM to 5 PM
17R6790HYPHypBXD2773M8/17/101:15 PM to 5 PM
18R6798HYPHypBXD2971F8/17/101:15 PM to 5 PM
19R6794HYPHypBXD2971M8/17/101:15 PM to 5 PM
20R6816HYPHypBXD3174F8/18/108:45AM to 12:30 PM
21R6802HYPHypBXD3173M8/17/101:15 PM to 5 PM
22R6916HYPHypBXD3281F8/18/101 PM to 6:45 PM
23R6846HYPHypBXD3281M8/18/108:45AM to 12:30 PM
24R6822HYPHypBXD3477F8/18/108:45AM to 12:30 PM
25R6808HYPHypBXD3477M8/18/108:45AM to 12:30 PM
26R6814HYPHypBXD3979M8/18/108:45AM to 12:30 PM
27R6848HYPHypBXD4085F8/18/108:45AM to 12:30 PM
28R6850HYPHypBXD4085M8/18/108:45AM to 12:30 PM
29R6810HYPHypBXD4287F8/18/108:45AM to 12:30 PM
30R6758HYPHypBXD4381M8/17/109:30 AM to 12:30AM
31R6746HYPHypBXD4483F8/17/109:30 AM to 12:30AM
32R6750HYPHypBXD4483M8/17/109:30 AM to 12:30AM
33R6764HYPHypBXD4577F8/17/109:30 AM to 12:30AM
34R6762HYPHypBXD4577M8/17/109:30 AM to 12:30AM
35R6880HYPHypBXD4876F8/18/101 PM to 6:45 PM
36R6882HYPHypBXD4876M8/18/101 PM to 6:45 PM
37R6748HYPHypBXD4984M8/17/109:30 AM to 12:30AM
38R6890HYPHypBXD5077F8/18/101 PM to 6:45 PM
39R6892HYPHypBXD5077M8/18/101 PM to 6:45 PM
40R6918HYPHypBXD5684F8/18/101 PM to 6:45 PM
41R6894HYPHypBXD5677M8/18/101 PM to 6:45 PM
42R6770HYPHypBXD6070F8/17/109:30 AM to 12:30AM
43R6772HYPHypBXD6070M8/17/101:15 PM to 5 PM
44R6836HYPHypBXD6283F8/18/108:45AM to 12:30 PM
45R6844HYPHypBXD6283M8/18/108:45AM to 12:30 PM
46R6888HYPHypBXD6377F8/18/101 PM to 6:45 PM
47R6878HYPHypBXD6584F8/18/101 PM to 6:45 PM
48R6874HYPHypBXD6584M8/18/101 PM to 6:45 PM
49R6930HYPHypBXD6876F8/18/101 PM to 6:45 PM
50R6932HYPHypBXD6876M8/18/101 PM to 6:45 PM
51R6776HYPHypBXD6969F8/17/101:15 PM to 5 PM
52R6774HYPHypBXD6980M8/17/101:15 PM to 5 PM
53R6926HYPHypBXD7076F8/18/101 PM to 6:45 PM
54R6922HYPHypBXD7076M8/18/101 PM to 6:45 PM
55R6870HYPHypBXD7176F8/18/101 PM to 6:45 PM
56R6872HYPHypBXD7176M8/18/101 PM to 6:45 PM
57R6778HYPHypBXD7383F8/17/101:15 PM to 5 PM
58R6780HYPHypBXD7383M8/17/101:15 PM to 5 PM
59R6838HYPHypBXD7576F8/18/108:45AM to 12:30 PM
60R6830HYPHypBXD7576M8/18/108:45AM to 12:30 PM
61R6934HYPHypBXD7987F8/18/101 PM to 6:45 PM
62R6782HYPHypBXD8073F8/17/101:15 PM to 5 PM
63R6784HYPHypBXD8073M8/17/101:15 PM to 5 PM
64R6914HYPHypBXD8381F8/18/101 PM to 6:45 PM
65R6912HYPHypBXD8381M8/18/101 PM to 6:45 PM
66R6834HYPHypBXD8476M8/18/108:45AM to 12:30 PM
67R6938HYPHypBXD8574F8/18/101 PM to 6:45 PM
68R6940HYPHypBXD8574M8/18/101 PM to 6:45 PM
69R6910HYPHypBXD8783F8/18/101 PM to 6:45 PM
70R6908HYPHypBXD8783M8/18/101 PM to 6:45 PM
71R6896HYPHypBXD8982F8/18/101 PM to 6:45 PM
72R6898HYPHypBXD8982M8/18/101 PM to 6:45 PM
73R6904HYPHypBXD9082F8/18/101 PM to 6:45 PM
74R6906HYPHypBXD9082M8/18/101 PM to 6:45 PM
75R6924HYPHypBXD9286F8/18/101 PM to 6:45 PM
76R6928HYPHypBXD9289M8/18/101 PM to 6:45 PM
77R6920HYPHypBXD9576F8/18/101 PM to 6:45 PM
78R6868HYPHypBXD9576M8/18/108:45AM to 12:30 PM
79R6900HYPHypBXD9771F8/18/101 PM to 6:45 PM
80R6902HYPHypBXD9771M8/18/101 PM to 6:45 PM
81R6876HYPHypBXD9977F8/18/101 PM to 6:45 PM
82R6884HYPHypBXD9977M8/18/101 PM to 6:45 PM
83R6840HYPHypBXD10083F8/18/108:45AM to 12:30 PM
84R6832HYPHypBXD10083M8/18/108:45AM to 12:30 PM
85R6944HYPHypBXD10189F8/18/101 PM to 6:45 PM
86R6942HYPHypBXD10189M8/18/101 PM to 6:45 PM
87R6756HYPHypBXD10288M8/17/109:30 AM to 12:30AM
88R6768HYPHypBXD10378F8/17/109:30 AM to 12:30AM
89R6766HYPHypBXD10378M8/17/109:30 AM to 12:30AM
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