From b2feda451ccfbeaed02dce9088d6dd228cf15861 Mon Sep 17 00:00:00 2001 From: Bonface Date: Tue, 13 Feb 2024 23:52:26 -0600 Subject: Update dataset RTF Files. --- general/datasets/HZI_LTCF_0313/processing.rtf | 1 - 1 file changed, 1 deletion(-) delete mode 100644 general/datasets/HZI_LTCF_0313/processing.rtf (limited to 'general/datasets/HZI_LTCF_0313/processing.rtf') diff --git a/general/datasets/HZI_LTCF_0313/processing.rtf b/general/datasets/HZI_LTCF_0313/processing.rtf deleted file mode 100644 index 5502082..0000000 --- a/general/datasets/HZI_LTCF_0313/processing.rtf +++ /dev/null @@ -1 +0,0 @@ -

Read Mapping and Gene Expression Quantification RNA-seq reads were quality-trimmed using Trim Galore (31) and mapped to the mm10 reference genome or to the IAV PR8M genome using STAR (32). Counts were summarized at the gene level using the R-package Rsubread (33), normalized and log transformed using the R-package DESeq2 (34), and batchcorrected using the ComBat function of the R-package sva (35, 36). For annotations of genes, ENTREZID from Rsubread were matched to RefSeq annotations using R-package biomart (37).

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