From e34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534 Mon Sep 17 00:00:00 2001
From: Bonface
Date: Thu, 15 Feb 2024 06:09:54 -0600
Subject: Update dataset RTF Files.

---
 general/datasets/HEIONCvsCRetILM6_0911/tissue.rtf | 32 -----------------------
 1 file changed, 32 deletions(-)
 delete mode 100644 general/datasets/HEIONCvsCRetILM6_0911/tissue.rtf

(limited to 'general/datasets/HEIONCvsCRetILM6_0911/tissue.rtf')

diff --git a/general/datasets/HEIONCvsCRetILM6_0911/tissue.rtf b/general/datasets/HEIONCvsCRetILM6_0911/tissue.rtf
deleted file mode 100644
index 766ab59..0000000
--- a/general/datasets/HEIONCvsCRetILM6_0911/tissue.rtf
+++ /dev/null
@@ -1,32 +0,0 @@
-<blockquote>
-<p><strong>Tissue preparation protocol</strong>. Animal were killed by rapid cervical dislocation. Retinas were removed immediately and placed in RNA<em>later</em> at room temperature. Two retinas from one mouse were stored in a single tube.</p>
-
-<p>Each array was hybridized with a pool of cRNA from 2 retinas (1 mouse). Natalie Freeman-Anderson extracted RNA at UTHSC.</p>
-
-<p>&nbsp;</p>
-
-<p><strong>Dissecting and preparing eyes for RNA extraction</strong></p>
-
-<p>&nbsp;</p>
-
-<p>Retinas for RNA extraction were placed in RNA STAT-60 (Tel-Test Inc.) and processed per manufacturer&rsquo;s instructions (in brief form below). Total RNA was extracted with RNA STAT-60 (Tel-Test Inc.) according to the manufacturer&#39;s instructions. Briefly we:</p>
-
-<p>&nbsp;</p>
-
-<ul>
-	<li>Homogenize tissue samples in the RNA STAT-60 (1 ml/50 to 100 mg tissue via syringe)</li>
-	<li>Allow the homogenate to stand for 5-10 min at room temperature</li>
-	<li>Add 0.2 ml of chloroform per 1 ml RNA STAT-60</li>
-	<li>Mix the sample vigorously for 15 sec and let the sample incubate at room temperature for 5-10 min</li>
-	<li>Centrifuge at 12,000 g for 1 hr at 4&deg;C</li>
-	<li>Transfer the aqueous phase to a clean centrifuge tube</li>
-	<li>Add 0.5 ml of isopropanol per 1 ml RNA STAT-60</li>
-	<li>Vortex and incubate the sample at -20&deg;C for 1 hr or overnight</li>
-	<li>Centrifuge at 12,000 g for 1 hr</li>
-	<li>Remove the supernatant and wash the RNA pellet with 75% ethanol</li>
-	<li>Remove ethanol, let air dry (5-10 min)</li>
-	<li>Dissolve the pellet in 50 &mu;l of nuclease free water.
-	<p>&nbsp;</p>
-	</li>
-</ul>
-</blockquote>
-- 
cgit v1.2.3