From b2feda451ccfbeaed02dce9088d6dd228cf15861 Mon Sep 17 00:00:00 2001 From: Bonface Date: Tue, 13 Feb 2024 23:52:26 -0600 Subject: Update dataset RTF Files. --- general/datasets/Eye_m2_0908_wtwt/processing.rtf | 3381 ++++++++++++++++++++++ 1 file changed, 3381 insertions(+) create mode 100644 general/datasets/Eye_m2_0908_wtwt/processing.rtf (limited to 'general/datasets/Eye_m2_0908_wtwt/processing.rtf') diff --git a/general/datasets/Eye_m2_0908_wtwt/processing.rtf b/general/datasets/Eye_m2_0908_wtwt/processing.rtf new file mode 100644 index 0000000..4349e60 --- /dev/null +++ b/general/datasets/Eye_m2_0908_wtwt/processing.rtf @@ -0,0 +1,3381 @@ +

Range of Gene Expression in the Eye. Expression of transcripts in the HEIMED and most other GN data sets is measured on a log2 scale. Each unit corresponding approximately to a 2-fold difference in hybridization signal intensity. To simplify comparisons among different data sets and cases, log2 RMA values of each array have been adjusted to an average expression of 8 units and a standard deviation of 2 units (variance stabilized). Values of all 45,101 probe sets in this data set range from a low of 4.8 (Tcf15, probe set 1420281_at) to a high of 15.5 (crystallin gamma C, Crygc, probe set 1422674_s_at). This corresponds to 10.7 units or a 1 to 1700 dynamic range of expression (2^10.73).

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We used pooled RNA samples of whole eyes, usually two independent pools--one male, one female pool--for most lines of mice. This data set was processed using the RMA protocol. A total of 2223 probes sets are associated with LRS values greater than 46 (LOD >10).

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We calibrated this log intensity scale using Affymetrix spike-in control probe sets. These 18 control probe sets target exogenous bacterial mRNAs that are added to each sample (a graded dose spike cocktail) during preparation at concentrations of 1.5, 5, 25, and 100 pM. (To find these probe sets, search GN’s ALL search field using the string “AFFX pM”.) A value of 6 or less is equivalent to an mRNA concentration of under 0.4 pM, a value of 8 is equivalent to ~1.5 pM, 9.5 is equivalent to ~5 pM, 11.5 is equivalent to ~25 pM, 13.5 is equivalent to ~100 pM, and a value of 15.5 is equivalent to an mRNA concentration of 400 pM or greater.

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This range can be converted to the mRNA molecules per cell in the eye assuming that a value of 8 is equivalent to about 1 mRNA copy per cell (Kanno et al. 2006, see http://www.biomedcentral.com/1471-2164/7/64). Since the expression of rhodopsin mRNA is normally 15 units, we predict that there are 27 or ~128 Rho mRNAs per cell in the whole eye and ~256 in rods themselves (assuming that rods make up about half of all cells in the eye). For this purpose it may be useful to know that a normal mouse eye contains between 6 and 8 million rod photoreceptors (Guo, Lu, and Williams; GN BXD Phenotype ID 11024).

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Note that some probe sets with very low expression still provide reliable data. For example, probe set 1440397_at (Cacna2d1) has expression of only 5.5 units (a value that would be declared as "absent" using conventional Affymetrix procedures), but the values for this calcium channel transcript are associated with a very strong cis QTL with an LRS of 79 (LOD = 17). This strong linkage is definitely not due to chance since the probability of the expression data mapping precisely to the location of the parent gene itself is about 10e-16. This indicates a high signal to noise ratio and the detection of significant strain variation of the correct transcript.

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The standard error of the mean for the HEIMED data set is computed for 2 to 6 biological replicates. The standard error of such small samples tends to systematically underestimate the population standard error. With n = 2 the underestimate is about 25%, whereas for n = 6 the underestimate is 5%. Gurland and Tripathi (1971) provide a correction and equation for this effect (see Sokal and Rohlf, Biometry, 2nd ed., 1981, p 53 for an equation of the correction factor for small samples of n < 20.) Probe (cell) level data from the CEL file: These CEL values produced by GCOS are 75% quantiles from a set of 91 pixel values per cell. The CEL files were processed using the RMA protocol. We processed the first three batches together. The last batch was processed separately and merged as described below.

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After RMA processing using Biobase affy10 build running under R version 2.7.1, all array data sets were rank-order normalized. This second round of quantile normalization removes much residual non-linearity across arrays and forces every array to have the same distribution of values as the mean of all arrays. Comparative array data quality was then evaluated in DataDesk. Outlier arrays were flagged by visual inspection in DataDesk, usually by means of an analysis of scatter plots and more quantitatively by generating a correlation matrix of all arrays. Those arrays with mean correlation <0.96 versus all other arrays indicates trouble or a biological outlier). In some cases, outliers were expected, such as samples from strains with retinal degeneration (FVB/NJ, NOD/LtJ, MOLF/EiJ, C3H/HeJ and BXD24), samples from wild subspecies such as WSB/EiJ, CAST/EiJ, PWD/PhJ, and PWK/PhJ, and knockouts. However, when arrays were anomolous both within strain and across strains, they were often simply discarded. The assumption is that anomolous data are much more likely due to experimental and technical errors than to informative biological variation. Approximately 10% of arrays were discarded.

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After this process, the acceptable set of arrays was renormalized using all step as above, starting with the original RMA procedure, etc.

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We reviewed the data set using a new method developed by RW Williams, Jeremy Peirce, and Hongqiang Li. For the full set of arrays that passed standard QC protocols described above, we computed the strain means for the BXD strains, B6, D2, and F1s. Using this set of strain means we then computed LRS scores for all 45101 probe sets and counted the number of transcripts that generated QTLs with LRS values greater than 50. This value (e.g., 1800) represented the QTL harvest for the full data set. We then dropped a single array from the data set, recomputed strain means, and recomputed the number of transcripts with LRS scores great than 50. This value is expected to typically reduce the number of QTLs that reach the criterion level (e.g., 1750 QTLs > 50). This process was repeated for every array to obtain an array-specific difference value--the effect of removing that array on the total QTL count. For example, the loss of a single array might cause a decrease in 50 QTLs. Values ranged from approximately -90 (good arrays) to +40 (bad arrays). This procedure is similar in some ways to a jackknife protocol, although we are not using this procedure to esimate an error term, but rather as a method to polish a data set.

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During this process we discovered that nearly 20 arrays in the batch 2 had been mislabeled at some point in processing. We computed the correct strain membership of each array using a large number of Mendelian probe sets (more than 50) and comparing their match to standard SNP and microsatellite markers and the original array data set of November 2005. This allowed us to rescue a large number of arrays that were of high quality.

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A third batch of approximately 40 arrays were processed by Yan Jiao and Weikuan Gu in August 2006. These complete data set assembled by Hongqiang Li. This process again included a correction for a batch effect.

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For the June 2006 data set Hongqiang Li used a new batch correction method that stabilizes the range of expression in each batch. For each of the three large batches, we extracted the minumum and maximum raw probe expression (CEL file level) value. We then adjusted raw probe values in each batch to have the same range as the first and largest batch (batch 1) using a simple linear interpolation. These procedures generated new correct CEL files which were then used with RMA to generate final probe set estimates.

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For the final fourth batch of arrays (Sept 2008) Arthur Centeno and Rob Williams corrected for a systematic difference in probe set expression values between original arrays run in 2005 and 2006 and the new arrays added in 2008 (n = 45 acceptable arrays). This difference is due to unknown technical batch effects that are probably associated with labeling, hybridization, and scanning. We performed a simple correction to normalize values of the new set of arrays to those of the old set (batches 1 through 3). No changes were made to any values of the previous three batches. We corrected only the probe set level (RMA) values and not the CEL files. For this final batch, we corrected for the difference (offset) in probe set expression between the first three batches arrays run in 2005 and 2006 (a total of 174 acceptable arrays) and the new batch (n = 47 acceptable arrays). This difference is due to unknown technical effects that are probably related to various steps in labeling, hybridization, and scanning. The correction was applied as follows: (1) RWW selected 51 high quality arrays with similar expression characteristics (r = 0.97 or better between pairs of arrays) in the old data set (from batches 1, 2, and 3) and 34 high quality arrays in the final batch. RWW used scatterplots of full RMA transcriptome data sets to review many pairs of arrays within these new and old array batches. Strains with retinal degeneration or unusual eye gene expression characteristics were excluded from these selected subsets. The average expression values for each probe set were then computed for both the old and new array subsets. The offset value (old minus new) was added to each probe set across all 47 new arrays. This processes forces the average probe set in the new arrays to be very close to that of the previous arrays.

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Table 2: Sample tube ID, strain, original CEL filename, and Affymetrix quality control values. Columns labeled Scale factor, Background Average, Present, Absent, Marginal and 3'/5' ratios for actin and Gapdh were collated from the Affymetrix Report (RPT) files.

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+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
IndexTubeIDStrainOriginal CELScale factorBackground AveragePresentAbsentMarginalAFFX-b-ActinMur (3'/5')AFFX-GapdhMur (3'/5')Batch IdUsed for batch control
1R2595E.1129S1/SvImJR2595E.1.CEL1.7911561.00%37.50%1.50%1.460.771Y
2R2533E.1129S1/SvImJR2533E.1.CEL2.119457.90%40.50%1.60%1.370.781Y
3R0754E.1A/JR0754E.1.CEL2.728659.80%38.70%1.50%1.360.761Y
4R4521EB6129P2F2N1-Clcn3R4521E.CEL4.8338.763.30%35.30%1.40%1.250.774 
5R4522EB6129P2F2N1-Clcn3R4522E.CEL5.7637.3662.90%35.70%1.40%1.370.834 
6R4523EB6129P2F2N1-Clcn3R4523E.CEL4.8840.4263.90%34.70%1.40%1.270.774 
7R4526EB6129P2F2N1-Gabbr1R4526E.CEL3.8444.1865.00%33.70%1.30%1.340.784Y
8R4509EB6129P2F2N1-Gabbr1R4509E.CEL7.4534.7658.90%39.70%1.40%1.450.834 
9R4510EB6129P2F2N1-Gabbr1R4510E.CEL8.4437.4457.40%41.10%1.50%1.350.834 
10R4511EB6129P2F2N1-Gabbr1R4511E.CEL5.9142.0261.40%37.20%1.40%1.410.834 
11R4524EB6129P2F2N1-Gabbr1R4524E.CEL5.4942.3462.40%36.20%1.40%1.290.784Y
12R4525EB6129P2F2N1-Gabbr1R4525E.CEL4.6941.363.10%35.50%1.40%1.270.84Y
13R4515EB6129P2F2N1-Gabra1R4515E.CEL5.7541.7662.80%35.80%1.40%1.410.814Y
14R4516EB6129P2F2N1-Gabra1R4516E.CEL7.0740.7360.20%38.40%1.40%1.320.874Y
15R4517EB6129P2F2N1-Gabra1R4517E.CEL5.4538.0962.70%35.80%1.40%1.340.824Y
16R4512EB6129P2F2N1-Gnb5R4512E.CEL6.5638.0259.90%38.70%1.50%1.330.834 
17R4513EB6129P2F2N1-Gnb5R4513E.CEL4.1541.663.40%35.10%1.50%1.340.824 
18R4514EB6129P2F2N1-Gnb5R4514E.CEL5.8639.261.20%37.30%1.50%1.340.814 
19R4518EB6129P2F2N1-Gpr19R4518E.CEL5.5838.962.60%36.00%1.30%1.390.794Y
20R4519EB6129P2F2N1-Gpr19R4519E.CEL5.9541.9161.30%37.30%1.40%1.350.844Y
21R2601E.1B6D2F1R2601E.1.CEL2.559258.90%39.60%1.50%1.440.781Y
22R2602E.1B6D2F1R2602E.1.CEL2.68459.70%38.80%1.50%1.370.781Y
23R1676E.1BALB/cByJR1676E.1.CEL2.699858.90%39.60%1.50%1.460.741 
24R1672E.1BALB/cByJR1672E.1.CEL2.2211159.90%38.60%1.50%1.260.81Y
25R4530EBALB/cJR4530E.CEL6.3737.5360.80%37.80%1.40%1.30.844Y
26R4529EBALB/cJR4529E.CEL5.7141.3360.50%38.00%1.50%1.480.84Y
27R2704E.2BXD1R2704E.2.CEL2.066139.6156.60%41.90%1.50%1.310.812 
28R2707E.3BXD1R2707E.3.CEL18056.40%42.10%1.50%1.430.793 
29R1231E.2BXD2R1231E.2.CEL2.197138.7357.30%41.30%1.40%1.410.772 
30R2598E.1BXD2R2598E.1.CEL1.9910660.90%37.60%1.50%1.270.781Y
31R2591E.1BXD5R2591E.1.CEL1.713658.50%40.00%1.50%1.330.781Y
32R2714E.2BXD5R2714E.2.CEL1.404144.3560.60%37.90%1.50%1.430.792 
33R2570E.1BXD6R2570E.1.CEL1.998758.50%40.00%1.50%1.460.761Y
34R2694E.2BXD6R2694E.2.CEL1.98397.2361.60%37.10%1.30%1.390.822 
35R2538E.1BXD8R2538E.1.CEL1.9110261.20%37.30%1.50%1.520.791Y
36R2709E.2BXD8R2709E.2.CEL1.9999.7960.90%37.60%1.50%1.420.762 
37R2708E.2BXD9R2708E.2.CEL1.966126.4657.70%40.70%1.50%1.40.842 
38R2569E.1BXD9R2569E.1.CEL1.758755.10%43.40%1.50%2.823.141 
39R2581E.1BXD11R2581E.1.CEL1.948962.10%36.40%1.60%1.550.811Y
40R2612E.2BXD11R2612E.2.CEL1.83142.0358.20%40.50%1.40%1.780.812 
41R2742E.2BXD12R2742E.2.CEL2.127134.1457.00%41.60%1.40%1.640.782 
42R2543E.1BXD12R2543E.1.CEL1.6111858.60%39.90%1.60%1.430.771Y
43R2586E.1BXD13R2586E.1.CEL2.017456.40%42.00%1.60%2.853.811 
44R877E.2BXD13R877E.2.CEL1.558125.6361.20%37.50%1.20%1.420.812 
45R2557E.1BXD14R2557E.1.CEL1.839962.50%36.10%1.40%1.310.781Y
46R1128E.2BXD14R1128E.2.CEL1.9111559.90%38.80%1.40%1.20.821Y
47R2701E.3BXD15R2701E.3.CEL18860.60%37.90%1.40%1.50.773 
48R2716E.2BXD15R2716E.2.CEL2.015150.8356.40%42.10%1.60%1.420.812 
49R2711E.2BXD16R2711E.2.CEL1.953118.5359.00%39.60%1.50%1.450.82 
50R2567E.1BXD16R2567E.1.CEL2.248256.70%41.60%1.70%1.370.751 
51R2720E.2BXD18R2720E.2.CEL2.3299.9359.50%39.00%1.50%1.330.772 
52R2559E.1BXD18R2559E.1.CEL1.6510460.80%37.70%1.50%1.270.781Y
53R2560E.1BXD19R2560E.1.CEL1.799860.90%37.50%1.60%1.350.81Y
54R2713E.2BXD19R2713E.2.CEL1.67120.8260.20%38.30%1.50%1.450.82 
55R2584E.1BXD20R2584E.1.CEL2.078459.30%39.10%1.60%1.40.761Y
56R2731E.2BXD20R2731E.2.CEL1.82514759.00%39.50%1.50%1.40.82 
57R2702E.2BXD21R2702E.2.CEL1.811128.6559.40%39.10%1.40%1.260.82 
58R2541E2.1BXD21R2541E2.1.CEL2.6312556.00%42.40%1.50%1.290.781 
59R2553E.1BXD22R2553E.1.CEL1.9511159.90%38.50%1.50%1.280.761Y
60R2700E.2BXD22R2700E.2.CEL1.858102.9661.50%37.10%1.30%1.480.792 
61R2558E-2.1BXD23R2558E-2.1.CEL2.233125.0558.60%39.90%1.50%1.430.772 
62R1086E.2BXD23R1086E.2.CEL2.233125.0558.60%39.90%1.50%1.430.772 
63R2719E.2BXD24R2719E.2.CEL1.47140.3861.50%37.20%1.30%1.380.792 
64R2589E2.1BXD24R2589E2.1.CEL2.6111257.50%40.90%1.60%1.240.81 
65R2573E-2.1BXD25R2573E-2.1.CEL3.157257.90%40.70%1.40%1.770.971 
66R2683E.2BXD25R2683E.2.CEL1.777115.6458.30%40.30%1.40%2.010.792 
67R2703E.2BXD27R2703E.2.CEL1.263134.7862.60%36.10%1.40%1.440.782 
68R2729E.3BXD27R2729E.3.CEL18757.90%40.60%1.50%1.560.843Y
69R2562E.3BXD28R2562E.3.CEL1.6511659.90%38.40%1.70%1.370.793Y
70R2721E.2BXD28R2721E.2.CEL2.065157.3956.10%42.40%1.50%1.310.812 
71R2561E.3BXD29R2561E.3.CEL17753.30%45.40%1.40%3.3619.663 
72R1258E.2BXD31R1258E.2.CEL2.063117.0959.00%39.50%1.50%1.540.782 
73R2597E.1BXD31R2597E.1.CEL2.379460.30%38.30%1.50%1.340.771Y
74R2563E.1BXD32R2563E.1.CEL1.5510261.90%36.70%1.40%1.50.81 
75R1216E.2BXD32R1216E.2.CEL2.23111.9958.80%39.80%1.40%1.350.792 
76R2542E.1BXD33R2542E.1.CEL2.139756.50%41.80%1.60%1.910.931 
77R857E.2BXD33R857E.2.CEL1.737113.9861.90%36.70%1.30%1.60.772 
78R1451E.2BXD34R1451E.2.CEL1.843140.0559.00%39.50%1.50%1.420.812Y
79R2585E.1BXD34R2585E.1.CEL2.647558.30%40.00%1.70%1.250.771 
80R2698E.3BXD36R2698E.3.CEL18659.70%39.00%1.30%1.460.783 
81R2705E.3BXD36R2705E.3.CEL18660.20%38.40%1.40%1.460.773 
82R2710E.2BXD38R2710E.2.CEL2.112122.158.80%39.80%1.40%1.370.782 
83R2532E.1BXD38R2532E.1.CEL2.049459.80%38.70%1.50%1.370.81Y
84R2574E.1BXD39R2574E.1.CEL1.989161.20%37.30%1.50%1.390.781 
85R2695E.2BXD39R2695E.2.CEL1.638122.760.80%37.80%1.50%1.420.82 
86R2699E.2BXD40R2699E.2.CEL1.827105.2361.70%36.90%1.40%1.420.812 
87R2590E.1BXD40R2590E.1.CEL2.717759.10%39.30%1.50%1.40.771Y
88R2696E.2BXD42R2696E.2.CEL1.622118.9562.00%36.60%1.50%1.530.792 
89R2596E.1BXD42R2596E.1.CEL2.6310859.00%39.60%1.50%1.240.81 
90R994E.2BXD43R994E.2.CEL1.966113.1260.80%37.80%1.40%1.660.82 
91R2607E.1BXD43R2607E.1.CEL2.4311558.60%40.00%1.40%1.310.761Y
92R2594E.1BXD44R2594E.1.CEL1.7711759.80%38.80%1.40%1.350.851 
93R2610E.2BXD44R2610E.2.CEL1.814142.9159.00%39.50%1.50%1.350.82 
94R2732E.2BXD45R2732E.2.CEL2.154122.4556.50%42.10%1.40%1.80.832 
95R2592E.1BXD45R2592E.1.CEL1.8510660.10%38.60%1.30%1.430.851Y
96R967E.2BXD48R967E.2.CEL1.948130.9557.30%41.20%1.50%1.630.812 
97R2606E.1BXD48R2606E.1.CEL2.5610658.90%39.70%1.40%1.350.831Y
98R2933E.3BXD50R2933E.3.CEL17252.90%45.60%1.50%2.450.983 
99R2937E.3BXD50R2937E.3.CEL18956.90%41.60%1.40%1.810.823 
100R2603E.1BXD51R2603E.1.CEL2.4911557.70%40.80%1.50%1.240.791 
101R1042E.2BXD51R1042E.2.CEL2.352104.1258.70%39.90%1.40%1.530.822 
102R2980E.3BXD55R2980E.3.CEL18256.90%41.70%1.50%1.770.843 
103R2690E.2BXD55R2690E.2.CEL1.887164.0156.10%42.30%1.60%1.430.82 
104R4176EBXD56R4176E.CEL4.7543.0863.00%35.60%1.30%1.390.814Y
105R4175EBXD56R4175E.CEL638.4961.30%37.30%1.40%1.470.814Y
106R1006E.3BXD60R1006E.3.CEL19854.90%43.70%1.50%2.70.863 
107R2725E.2BXD60R2725E.2.CEL1.551148.0159.80%38.80%1.40%1.430.792 
108R1074E.3BXD60R1074E.3.CEL111855.50%43.10%1.40%1.960.813 
109R2534E2.1BXD61R2534E2.1.CEL2.4711857.90%40.60%1.50%1.420.791 
110R2684E.2BXD61R2684E.2.CEL2.01131.0357.00%41.50%1.50%1.340.782 
111R1107E.3BXD62R1107E.3.CEL18355.20%43.40%1.40%2.430.933 
112R2681E.2BXD62R2681E.2.CEL2.086148.2457.20%41.30%1.50%1.290.812 
113R965E.3BXD62R965E.3.CEL193.5553.30%45.20%1.50%3.110.943 
114R1425E.2BXD63R1425E.2.CEL1.713659.30%39.30%1.40%1.430.822 
115R2576E.3BXD63R2576E.3.CEL18461.30%37.40%1.40%1.480.763 
116R943E-2.2BXD64R943E-2.2.CEL1.591141.3460.10%38.40%1.50%1.320.762 
117R2611E.1BXD64R2611E.1.CEL2.299258.00%40.50%1.50%1.571.061 
118R2689E.2BXD65R2689E.2.CEL1.721142.4459.90%38.60%1.50%1.380.762 
119R2583E.1BXD65R2583E.1.CEL2.497056.90%41.50%1.60%1.671.011 
120R2728E.2BXD66R2728E.2.CEL1.714137.4559.40%39.00%1.60%1.380.792 
121R2536E2.1BXD66R2536E2.1.CEL2.7410956.10%42.30%1.70%1.280.791 
122R1207E.2BXD66R1207E.2.CEL1.681136.8660.40%38.10%1.50%1.450.772 
123R1192E.2BXD67R1192E.2.CEL2.126123.3757.90%40.60%1.50%1.50.82 
124R2727E.3BXD67R2727E.3.CEL182.5556.10%42.40%1.50%1.970.872 
125R2691E.3BXD67R2691E.3.CEL19054.80%43.80%1.50%2.610.813 
126R2551E.1BXD68R2551E.1.CEL2.499254.30%44.10%1.60%2.911.551 
127R2726E.2BXD68R2726E.2.CEL1.811153.0958.70%39.80%1.50%1.390.782 
128R2593E.1BXD69R2593E.1.CEL1.6712859.20%39.50%1.30%1.470.921Y
129R975E.2BXD70R975E.2.CEL1.841137.9758.00%40.50%1.40%1.360.792 
130R2537E2.1BXD70R2537E2.1.CEL2.939958.00%40.50%1.60%1.290.751 
131R4531EBXD71R4531E.CEL4.7743.4862.40%36.30%1.40%1.230.774Y
132R4532EBXD71R4532E.CEL5.8940.6860.90%37.60%1.50%1.240.794Y
133R2779E.2BXD73R2779E.2.CEL1.746121.1159.60%39.00%1.40%1.50.82 
134R3024E.3BXD73R3024E.3.CEL178.0551.70%46.60%1.70%2.30.943 
135R2565E.1BXD75R2565E.1.CEL1.7910258.00%40.50%1.50%2.313.471 
136R1397E-re.2BXD75R1397E-re.2.CEL1.449189.7159.60%39.00%1.40%1.390.822 
137R2687E.3BXD77R2687E.3.CEL18058.00%40.60%1.40%1.570.83Y
138R2717E.2BXD77R2717E.2.CEL1.79784.4361.60%36.90%1.40%1.490.762 
139R1421E.3BXD77R1421E.3.CEL19452.40%46.20%1.40%2.290.823 
140R2579E.1BXD80R2579E.1.CEL2.427259.20%39.40%1.50%1.730.821 
141R2686E.2BXD80R2686E.2.CEL2.342119.6356.00%42.60%1.50%1.380.792 
142R2956E.3BXD83R2956E.3.CEL18455.40%43.20%1.40%1.390.843 
143R2960E.3BXD83R2960E.3.CEL18056.60%41.90%1.50%1.50.823Y
144R2922E.3BXD84R2922E.3.CEL19157.80%40.80%1.50%1.470.833Y
145R2895E.3BXD84R2895E.3.CEL17558.30%40.20%1.50%1.560.773Y
146R2692E.2BXD85R2692E.2.CEL1.423160.8760.20%38.30%1.40%1.460.792 
147R2715E.2BXD85R2715E.2.CEL1.488142.661.20%37.30%1.40%1.50.782 
148R1405E.2BXD86R1405E.2.CEL2.351119.3456.40%42.20%1.40%1.640.812 
149R1225E.3BXD86R1225E.3.CEL17153.90%44.60%1.40%3.21.613 
150R2724E.2BXD87R2724E.2.CEL1.906113.7160.70%37.90%1.40%1.450.792 
151R2540E.1BXD87R2540E.1.CEL2.339361.10%37.40%1.40%1.220.811Y
152R1433E.2BXD89R1433E.2.CEL12.24157.70%40.80%1.50%1.410.782 
153R2546E.1BXD89R2546E.1.CEL1.999658.60%39.70%1.70%1.470.781 
154R2578E2.1BXD90R2578E2.1.CEL2.799258.60%39.80%1.60%1.520.771Y
155R859E.2BXD90R859E.2.CEL1.847152.2257.90%40.70%1.40%1.360.772 
156R2682E.2BXD92R2682E.2.CEL1.547156.3160.40%38.20%1.40%1.370.772 
157R1388E.3BXD92R1388E.3.CEL16360.00%38.60%1.40%1.851.033 
158R1322E.3BXD92R1322E.3.CEL18055.90%42.60%1.50%1.750.743 
159R2733E.2BXD96R2733E.2.CEL1.7113.9962.10%36.60%1.30%1.40.782 
160R2554E.1BXD96R2554E.1.CEL2.189360.20%38.30%1.50%1.460.771Y
161R2649E.2BXD97R2649E.2.CEL2.343119.0457.50%41.20%1.40%1.530.82 
162R2577E.1BXD97R2577E.1.CEL2.077759.50%39.10%1.40%1.871.291 
163R2645E.3BXD98R2645E.3.CEL18859.40%39.20%1.50%1.590.813Y
164R2688E.2BXD98R2688E.2.CEL1.772145.2458.50%40.00%1.50%1.480.812 
165R4533EBXD99R4533E.CEL137.6960.30%38.20%1.40%1.330.894Y
166R4534EBXD99R4534E.CEL5.6936.6262.90%35.70%1.40%1.160.84Y
167R2885E.3BXSB/MpJR2885E.3.CEL17658.10%40.60%1.30%1.881.063 
168R2883E.3BXSB/MpJR2883E.3.CEL17156.40%42.00%1.50%1.590.843Y
169R1700E.1C3H/HeJR1700E.1.CEL2.986960.80%37.90%1.40%1.480.781 
170R1704E.1C3H/HeJR1704E.1.CEL2.588860.10%38.60%1.30%1.380.841 
171R2605E.1C57BL/6JR2605E.1.CEL1.8213160.50%38.20%1.30%1.320.81Y
172R0871EC57BL/6JR0871E.CEL6.2437.3861.90%36.70%1.40%1.410.84Y
173R0872E.1C57BL/6JR0872E.1.CEL3.138958.90%39.60%1.50%1.30.791Y
174R0872EC57BL/6JR0872E.CEL3.12888.5858.90%39.60%1.50%1.30.791 
175R4507EC57BL/6J-NyxR4507E.CEL8.1337.559.30%39.30%1.40%1.320.84Y
176R4508EC57BL/6J-NyxR4508E.CEL6.3337.2660.90%37.80%1.30%1.240.824Y
177R4505EC57BL/6J-Rpe65R4505E.CEL5.9837.4861.80%36.80%1.40%1.450.854Y
178R4506EC57BL/6J-Rpe65R4506E.CEL6.9437.961.10%37.50%1.30%1.50.834Y
179R4535EC57BLKS/JR4535E.CEL6.5937.2861.20%37.30%1.40%1.260.834Y
180R4536EC57BLKS/JR4536E.CEL140.7160.30%38.20%1.50%1.250.774Y
181R2564E.1CAST/EiJR2564E.1.CEL1.948958.50%39.90%1.60%1.60.771 
182R2580E.1CAST/EiJR2580E.1.CEL2.099558.20%40.10%1.70%1.40.761 
183R4537ECBA/CaJR4537E.CEL138.4560.60%37.90%1.50%1.630.824Y
184R4538ECBA/CaJR4538E.CEL5.8939.1861.70%36.90%1.40%1.450.84Y
185R4539ECZECHII/EiJR4539E.CEL7.7337.158.30%40.10%1.50%1.70.954Y
186R4540ECZECHII/EiJR4540E.CEL11.0436.6953.00%45.30%1.70%1.831.324 
187R2600E.1D2B6F1R2600E.1.CEL2.479558.10%40.20%1.70%1.410.781Y
188R2604E.1D2B6F1R2604E.1.CEL2.669059.40%39.20%1.50%1.280.791Y
189R1002E.3DBA/2JR1002E.3.CEL110254.80%43.70%1.50%2.840.833 
190R4541EDBA/2JR4541E.CEL143.461.40%37.00%1.50%1.370.734Y
191R959E.3DBA/2JR959E.3.CEL189.9753.20%45.30%1.50%3.661.094 
192R2572E.1DBA/2JR2572E.1.CEL2.417955.50%42.90%1.60%1.370.791 
193R4542EDBA/2JR4542E.CEL5.739.9561.00%37.40%1.50%1.230.814Y
194R2771E.3FVB/NJR2771E.3.CEL17055.30%43.20%1.50%1.690.833 
195R2772E.3FVB/NJR2772E.3.CEL17655.20%43.40%1.40%2.131.023 
196R2636E.1KK/HlJR2636E.1.CEL2.619358.90%39.50%1.50%1.390.761Y
197R2637E.1KK/HlJR2637E.1.CEL2.1910359.40%39.00%1.50%1.30.791Y
198R0999E.1LG/JR0999E.1.CEL2.458259.40%39.10%1.50%1.380.791Y
199R1004E.1LG/JR1004E.1.CEL2.449258.70%39.80%1.50%1.380.791Y
200R4543ELP/JR4543E.CEL6.5741.9960.30%38.20%1.50%1.280.754Y
201R4544ELP/JR4544E.CEL4.5639.962.40%36.10%1.50%1.230.774Y
202R2858E.3MOLF/EiJR2858E.3.CEL16453.80%44.70%1.50%1.590.953 
203R2919.3MOLF/EiJR2919.3.CEL16452.40%46.00%1.60%2.151.073 
204R1688E.1NOD/LtJR1688E.1.CEL2.669858.60%39.90%1.50%1.260.81Y
205R2566E-2.1NOD/LtJR2566E-2.1.CEL3.036959.80%38.80%1.50%1.380.751Y
206R4545ENZB/BlNJR4545E.CEL4.2343.4862.10%36.40%1.50%1.330.764Y
207R4546ENZB/BlNJR4546E.CEL6.2744.2259.40%39.10%1.50%1.170.824Y
208R2535E.1NZO/HlLtJR2535E.1.CEL1.898660.40%38.20%1.40%1.410.851 
209R2550E.1NZO/HlLtJR2550E.1.CEL1.798760.70%37.80%1.50%1.520.821 
210R2817E.3NZW/LacJR2817E.3.CEL15950.90%47.60%1.50%3.591.483 
211R2810ENZW/LacJR2810E.CEL       3 
212R2810E.3NZW/LacJR2810E.3.CEL17457.00%41.70%1.40%2.151.034Y
213R4547EPANCEVO/EiJR4547E.CEL5.2751.3457.20%41.10%1.70%1.70.834 
214R4548EPANCEVO/EiJR4548E.CEL10.5437.3950.30%48.00%1.70%1.681.094 
215R2635E.1PWD/PhJR2635E.1.CEL3.728054.20%44.10%1.70%1.530.851 
216R2634E.1PWD/PhJR2634E.1.CEL3.299055.90%42.50%1.60%1.570.811 
217R2544E.1PWK/PhJR2544E.1.CEL2.210854.90%43.50%1.70%1.360.821 
218R2549E.1PWK/PhJR2549E.1.CEL2.288457.30%41.20%1.50%1.570.831 
219R4550ESJL/JR4550E.CEL5.3540.4462.30%36.20%1.40%1.240.794 
220R2368E.1WSB/EiJR2368E.1.CEL2.578659.50%39.10%1.40%1.290.741Y
221R2547E.1WSB/EiJR2547E.1.CEL2.149058.20%40.10%1.60%1.320.771Y
+
-- cgit v1.2.3