From e34e7da50fc0ff5ed41e8bdaf2b1d41c9e9cf534 Mon Sep 17 00:00:00 2001 From: Bonface Date: Thu, 15 Feb 2024 06:09:54 -0600 Subject: Update dataset RTF Files. --- general/datasets/EYE_M2_1105_P/processing.rtf | 11 ----------- 1 file changed, 11 deletions(-) delete mode 100644 general/datasets/EYE_M2_1105_P/processing.rtf (limited to 'general/datasets/EYE_M2_1105_P/processing.rtf') diff --git a/general/datasets/EYE_M2_1105_P/processing.rtf b/general/datasets/EYE_M2_1105_P/processing.rtf deleted file mode 100644 index ff25e0d..0000000 --- a/general/datasets/EYE_M2_1105_P/processing.rtf +++ /dev/null @@ -1,11 +0,0 @@ -
Probe (cell) level data from the CEL file: These CEL values produced by GCOS are 75% quantiles from a set of 91 pixel values per cell. - --- cgit v1.2.3-
-- Step 1: We added an offset of 1.0 unit to each cell signal to ensure that all values could be logged without generating negative values. We then computed the log base 2 of each cell.
-- Step 2: We performed a quantile normalization of the log base 2 values for the total set of arrays using the same initial steps used by the RMA transform.
-- Step 3: We computed the Z scores for each cell value.
-- Step 4: We multiplied all Z scores by 2.
-- Step 5: We added 8 to the value of all Z scores. The consequence of this simple set of transformations is to produce a set of Z scores that have a mean of 8, a variance of 4, and a standard deviation of 2. The advantage of this modified Z score is that a two-fold difference in expression level corresponds approximately to a 1 unit difference.
-- Step 7: Finally, when appropriate, we computed the arithmetic mean of the values for the set of microarrays for each strain. Technical replicates were averaged before computing the mean for independent biological samples.
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