From b2feda451ccfbeaed02dce9088d6dd228cf15861 Mon Sep 17 00:00:00 2001 From: Bonface Date: Tue, 13 Feb 2024 23:52:26 -0600 Subject: Update dataset RTF Files. --- general/datasets/EPFL_LISPBXDHeHFD0114/tissue.rtf | 1 - 1 file changed, 1 deletion(-) delete mode 100644 general/datasets/EPFL_LISPBXDHeHFD0114/tissue.rtf (limited to 'general/datasets/EPFL_LISPBXDHeHFD0114/tissue.rtf') diff --git a/general/datasets/EPFL_LISPBXDHeHFD0114/tissue.rtf b/general/datasets/EPFL_LISPBXDHeHFD0114/tissue.rtf deleted file mode 100644 index eebcb20..0000000 --- a/general/datasets/EPFL_LISPBXDHeHFD0114/tissue.rtf +++ /dev/null @@ -1 +0,0 @@ -

Hearts were later shattered in liquid nitrogen, usually broken into 2-4 pieces, and around half of the sample (at random) was taken for preparation. All ~5 animals per cohort had their RNA prepared, and then were pooled evenly (by µg of RNA) into a single RNA sample for each cohort, thus differences due to different segments of the heart being taken should be mitigated, although not eliminated, and researchers examining extremely specific transcriptional regulation in the heart should keep this in mind. The pooled RNA samples were then purified using RNEasy, then sent out for array analysis. All RIN values were > 8.0.

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