From d029d5d7f8ead1f1de8d318045004a4a6f68f5fb Mon Sep 17 00:00:00 2001 From: Bonface Date: Fri, 9 Feb 2024 09:41:28 -0600 Subject: Update dataset RTF Files. --- general/datasets/EPFLBXDprotCDRPN0513/cases.rtf | 1 + 1 file changed, 1 insertion(+) create mode 100644 general/datasets/EPFLBXDprotCDRPN0513/cases.rtf (limited to 'general/datasets/EPFLBXDprotCDRPN0513/cases.rtf') diff --git a/general/datasets/EPFLBXDprotCDRPN0513/cases.rtf b/general/datasets/EPFLBXDprotCDRPN0513/cases.rtf new file mode 100644 index 0000000..ed4ced8 --- /dev/null +++ b/general/datasets/EPFLBXDprotCDRPN0513/cases.rtf @@ -0,0 +1 @@ +

40 strains of the BXD family (BXD43 – BXD103) and both parental strains (C57BL/6 and DBA/2) were born and raised at the EPFL in Switzerland prior to inclusion in this study. For each strain, 10 male animals were born and then separated evenly into two cohorts at 8 weeks of age: 5 animals per strain on a chow diet (6% kcal/fat, 20% protein, 74% carbohydrate) and 5 animals per strain on high fat diet (60% kcal/fat, 20% protein, 20% carbohydrate). Animals adjusted to the diet for 8 weeks, and then an intensive phenotyping metabolic phenotyping protocol was followed from 16 to 24 weeks of age (respiration, cold tolerance, oral glucose response, VO2max exercise, voluntary exercise, basal activity). Animals were communally housed until the last 5 weeks of the experiment, when the animals could rest. Animals were fasted overnight prior to sacrifice, which occurred between 9am and 11am after isoflurane anesthesia and perfusion. The gall bladder was removed, and then the livers were immediately frozen in liquid nitrogen.

-- cgit v1.2.3