From d029d5d7f8ead1f1de8d318045004a4a6f68f5fb Mon Sep 17 00:00:00 2001 From: Bonface Date: Fri, 9 Feb 2024 09:41:28 -0600 Subject: Update dataset RTF Files. --- general/datasets/EL_BXDCDHFDScWAT_0216/tissue.rtf | 11 +++++++++++ 1 file changed, 11 insertions(+) create mode 100644 general/datasets/EL_BXDCDHFDScWAT_0216/tissue.rtf (limited to 'general/datasets/EL_BXDCDHFDScWAT_0216/tissue.rtf') diff --git a/general/datasets/EL_BXDCDHFDScWAT_0216/tissue.rtf b/general/datasets/EL_BXDCDHFDScWAT_0216/tissue.rtf new file mode 100644 index 0000000..e595c55 --- /dev/null +++ b/general/datasets/EL_BXDCDHFDScWAT_0216/tissue.rtf @@ -0,0 +1,11 @@ +
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Subcuteaneous WAT was later shattered in liquid nitrogen, and around 100 mg was taken for preparation. All ~5 animals per cohort had their RNA prepared, and then were pooled evenly (by µg of RNA) into a single RNA sample for each cohort. The pooled RNA samples were then purified using RNEasy, then sent out for array analysis. All RIN values were > 8.0. RNA was prepared in the summer of 2013, while the RNEasy cleanup occurred in the winter of 2015; unlike the other tissues run as a part of this study, these RNA samples spent a significant time in the -80° freezers. However, the samples only underwent one freeze—thaw cycle.

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